Screening of Paenibacillus Larvae Spores in Apiaries from Eastern Poland. Nationwide Survey. Part I

Krystyna Pohorecka 1 , Marta Skubida 1 , Andrzej Bober 1  and Dagmara Zdańska 1
  • 1 Department of Honey Bee Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Abstract

Screening of the prevalence of Paenibacillus larvae spores in honey bee colonies in apiaries from 162 districts, belonging to nine provinces was carried out during 2009-2011. The honey samples were examined by the use of a culture method. Based on the number of CFUs grown on Columbia sheep blood agar medium, the level of infection and probability of American foulbrood outbreak was estimated. Altogether, 6,510 pooled honey samples from 32,550 bee colonies located in 2,294 apiaries were collected. P. larvae was identified in 45% of the surveyed apiaries. The widest distribution of P. larvae was found in the Małopolskie province. Culture-positive honey samples were obtained for 71% of the apiaries and in a half of them, the level of spores was high. In the Warmińsko-Mazurskie province, the presence of the bacterium was detected in 58% of the apiaries. In the remaining provinces, from 26% to 47% of the apiaries were contaminated with P. larvae spores

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  • 1. Antúnez K., D’Alessandro B., Piccini C., Corbella E., Zunino P.: Paenibacillus larvae larvae spores in honey samples from Uruguay: a nationwide survey. J Invertebr Pathol, 2004, 86, 56-58.

  • 2. Genersch E., Ashiralieva A., Fries I.: Strain- and genotype-specific differences in virulence of Paenibacillus larvae subsp. larvae, a bacterial pathogen causing American foulbrood disease in honeybees. Appl Environ Microbiol 2005, 71, 7551-7555.

  • 3. Genersch E., Fosgren E., Pentikäinen J., Ashiralieva A., Rauch S., Kilwinski J., Fries I.: Reclassification of Paenibacillus larvae subsp. pulvifaciens and Paenibacillus larvae subsp. larvae as Paenibacillus larvae without subspecies differentiation. Int J Syst Evol Microbiol 2006, 56, 501-511.

  • 4. Gochnauer T.A., Corner J.: Detection and identification of Bacillus larvae in commercial sample of bee-collected pollen. J Apic Res 1974, 13, 265-267.

  • 5. Goodwin R.M., Perry J.H., Haine H.M.: A study on the presence of Bacillus larvae spores carried by adult honeybees to identify colonies with clinical symptoms of American foulbrood disease. J Apic Res 1996, 35, 118-120.

  • 6. de Graff D.C., Vandekerchove D., Dobbelaere W., Peeters J.E., Jacobs F.J.: Influence of the proximity of American foulbrood cases and apicultural management on the prevalence of Paenibacillus larvae spores in Belgian honey. Apidologie 2001, 32, 587-599.

  • 7. Hansen H.: Methods for determining the presence of the foulbrood bacterium Bacillus larvae in honey. Danish J Plant Soil Sci 1984, 88, 325-328.

  • 8. Hansen H., Brodsgaard C.J.: American foulbrood: a review of its biology, diagnosis and control. Bee World 1999, 80, 5-23.

  • 9. Hornitzky M.A.Z., Clark S.: Culture of Bacillus larvae from bulk honey samples for the detection of American foulbrood. J Apic Res 1991, 30, 13-16.

  • 10. Lindström A., Fries I.: Sampling of adult bees for detection of American foulbrood (Paenibacillus larvae subsp. larvae) spores in honey bee (Apis mellifera). J Apic Res 2005, 44, 82-86.

  • 11. Lipiński Z., Szubstarski J., Szubstarska D., Kasztelewicz J.: The contamination of winter stores and early spring honey with spores of Paenibacillus larvae larvae in Polish apiaries of the Małopolska - province. Pol J Vet Sci 2007, 10, 71-74.

  • 12. Nordström S., Forsgren E., Fries I.: Comparative diagnosis of American foulbrood using samples of adult honey bees and honey. J Apic Sci 2002, 46, 5-13.

  • 13. von der Ohe W.: Control of American foulbrood by using alternatively eradication method and artificial swarmas. Apiacta 2003, 38, 137-139.

  • 14. von der Ohe W., Dustman J.H.: Efficient prophylactic measures against AFB by bacteriological analysis of honey for spore contamination. Am Bee J 1997, 137, 171-191.

  • 15. von der Ohe W., Schütze K., Lienau F.W.: Arealuntersuchungen auf Bacillus larvae-Sporen im Honig als Prophylaktikum. Apidologie 1996, 27, 277-279.

  • 16. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.2.2. OIE, Paris, 2010.

  • 17. Otten Ch., Otto A.: Epidemiology and control of the American foulbrood in Germany. Apiacta 2005, 40, 16-22.

  • 18. Pernal S.F., Melathopoulos A.P.: Monitoring for American foulbrood spores from honey and bee samples in Canada. Apiacta 2006, 41, 99-109.

  • 19. Pohorecka K., Bober A.: Occurrence of Paenibacillus larvae spores in honey samples from domestic apiaries. J Apicult Sci 2008, 52, 105-111.

  • 20. Ritter W.: Early detection of American Foulbrood by honey and wax analysis. Apiacta 2003, 38, 125-130.

  • 21. Semkiw P., Skubida P.: Evaluation of the economical aspects of Polish beekeeping. J Apic Sci 2010, 54, 5-15.

  • 22. Shimanuki H., Knox D.A.: Improved method for the detection of Bacillus larvae spores in honey. Am Bee J 1988, 128, 353-354.

  • 23. Shimanuki H., Knox D.A.: Diagnosis of honey bee diseases. USDA Handbook No. AH 690, Washington, 2000, p. 61.

  • 24. Steinkraus K.H., Morse R.A.: American foulbrood incidence in some US and Canadian honeys. Apidologie 1992, 23, 497-501.

  • 25. Titera D., Haklova M.: Detection method of Paenibacillus larvae larvae from beehive winter debris. Apiacta 2003, 38, 131-133.

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