Occurrence of Genes Encoding Virulence Factors in Bordetella Bronchiseptica Strains Isolated from Infected and Healthy Pigs

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Abstract

The objective of the study was to determine genotypic profiles of Bordetella bronchiseptica (Bbr) strains, based on the occurrence of genes encoding virulence factors, such as flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. 209 tested Bbr strains were obtained from Polish swine herds with different health status (with progressive atrophic rhinitis - PAR, suspected for PAR, and unknown). In total, seven different Bbr genotypes were determined. In 39.2% of Bbr isolates all three genes were present. In 41.1% of the isolates only two genes were detected. The most common genotype dnt+bfrZ-fla+ was present in 60 (28.5%) Bbr strains, 65% of them were obtained from farms with PAR. Twenty five (12%) Bbr isolates were identified as dnt-bfrZ+fla+ genotype and, as above, they were more frequently isolated from clinical cases of disease (84%). Among 31 (14.8%) strains only fla gene was evident, and in nine (4.3%) only dnt gene was present. There were no Bbr strains with bfrZ gene only. These results confirm the heterogenicity among Bbr strains.

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  • 1. Bemis D.A. Fenwick B.: Bordetella. In: Pathogenesis ofbacterial infections in animals. edited by Gyles C.L. Prescott J.F. Songer J.G. Thoen C.O. Blackwell Publishing USA 2010 pp. 259-272.

  • 2. Boschwitz J.S. van der Heide H.G.J. Mooi F.R. Relman D.A.: Bordetella bronchiseptica expresses the fimbrial structural subunit gene fimA. J Clin Microbiol 1997 179 7882-7885.

  • 3. Brickman T.J. Anderson M.T. Armstrong S.K.: Bordetella iron transport and virulence. Biometals 2007 20 303-322.

  • 4. Brockmeier S.L. Register K.B.: Expression of the dermonecrotic toxin by Bordetella bronchiseptica is not necessary for predisposing to infection with toxigenic Pasteurella multocida. Vet Microbiol 2007 125 284-289.

  • 5. Forde C.B. Shi X. Li J. Roberts M.: Bordetellabronchiseptica-mediated cytotoxicity to macrophages is dependent on bvg-regulated factors including pertactin. Infect Immun 1999 67 5972-5978.

  • 6. Hozbor D. Fouque F. Guiso N.: Detection of Bordetellabronchiseptica by polymerase chain reaction. Res Microbiol 1999 150 333-341.

  • 7. Irie Y. Yukl M. H.: In vivo colonization profile study of Bordetella bronchiseptica in the nasal cavity. FEMS Microbiol Lett 2007 275 191-198.

  • 8. Jong-de M.F.: Progressive and nonprogressive atrophic rhinitis. In: Straw B.E. Zimmerman J.J. Allaire S.D. Taylor D.J.: Diseases of Swine. Blackwell Publishing Ames USA 2006 pp. 577-602.

  • 9. Jungnitz H. West N.P. Walker M.J. Chhatwal G.S. Guzmán C.A.: A second two-component regulatory system of Bordetella bronchiseptica required for bacterial resistance to oxidative stress production of acid phosphatase and in vivo persistence. Infect Immun 1998 66 4640-4650.

  • 10. Kume K. Nakai T. Samejima Y. Sugimoto C.: Properties of dermonecrotic toxin prepared from sonic extracts of Bordetella bronchiseptica. Infect Immun 1986 52 370-377.

  • 11. Mattoo S. Miller J.F. Cotter P.A.: Role of Bordetellabronchiseptica fimbriae in tracheal colonization and development of a humoral immune response. Infect Immun 2000 68 2024-2033.

  • 12. Murphy E.R. Sacco R.E. Dickenson A. Metzger D.J. Hu Y. Orndorff P.E. Connell T.D.: BhuR a virulenceassociated outer membrane protein of Bordetella avium is required for the acquisition of iron from heme and hemoprotein. Infect Immun 2002 70 5390-5403.

  • 13. Nicholson M.L. Beall B.: Disruption of tonB in Bordetella bronchiseptica and Bordetella pertussis prevents utilization of ferric siderophores haemin and haemoglobin as iron sources. Microbiol 1999 145 2453-2461.

  • 14. OIE Terrestrial Manual 2008 Atrophic rhinitis of swine Paris 2008 pp. 1083-1091.

  • 15. Parkhill J. Sebaihia M. Preston A. Murphy L.D. Thompson N. Harris D.E. Holden M.T.G. Churcher C.M. Bentley S.D. Mungall K.L. Cerdeño-Tárraga A.M. Temple L. James K. Harris B. Quail M.A. Achtman M. Atkin R. Baker S. Basham D. Bason N. Cherevach I. Chillingworth T. Coolins M. Cronin A. Davis P. Doggett J. Felthwell T. Goble A. Hamlin N. Hauser H. Holroyd S. Jagels K. Leather S. Moule S. Norberczak H. O’Neil S. Ormond D. Price C. Rabbinowitsch E. Rutter S. Sanders M. Saunders D. Seeger K. Sharp S. Simmonds M. Skelton J. Squares R. Squares S. Stevens K. Unwin L. Whitehead S. Barrell B.G. Maskell D.: Comparative analysis of genome sequences of Bordetella pertussis Bordetellaparapertussis and Bordetella bronchiseptica. Nature Gen 2003 35 1-9.

  • 16. Parton R.: Bordetella. In: Topley & Wilson’s Microbiology & Microbial infections. Vol. 2. Bacteriology London 2005 pp. 1786-1817.

  • 17. Passerini de Rossi B.N. Friedman L.E. Belzoni C.B. Savino S. Arico B. Rappuoli R. Masignani V. Franco M.A.: Vir90 a virulence-activated gene coding for a Bordetella pertussis iron-regulated outer membrane protein. Res Microbiol 2003 154 443-450.

  • 18. Pradel E. Locht C.: Expression of the putative siderophore receptor gene bfrZ is controlled by the extracytoplasmic-function sigma factor BupI in Bordetella bronchiseptica. J Bacteriol 2001 183 2910-2917.

  • 19. Pullinger G.D. Adams T.E. Mullan T.I. Garrod T.I. Lax A.J.: Cloning expression and molecular characterization of the dermonecrotic toxin gene of Bordetella spp. Infect Immun 1996 64 4163-4171.

  • 20. Register K.B. Ackerman M.R.: A highly adherent phenotype associated with virulent Bvg1-phase swine isolates of Bordetella bronchiseptica grown under modulating conditions. Infect Immun 1997 65 5295-5300.

  • 21. Savelkoul P.H. de Kerf D.P.G. Willems R.J. Mooi F.R. van der Zeijst B.A. M. Gaastra W.: Characterization of the fim2 and fim3 fimbrial subunit genes of Bordetellabronchiseptica: roles of Fim2 and Fim3 fimbriae and flagella in adhesion. Infect Immun 1996 64 5098-5105.

  • 22. Schalk I.J. Yue W.W. Buchanan S.K.: Recognition of iron-free siderophores by TonB-dependent iron transporters. Mol Microbiol 2004 54 14-22.

  • 23. Stępniewska K. Markowska-Daniel I.: Evaluation of PCR test for detection of dermonecrotoxin of Bordetellabronchiseptica. Bull Vet Inst Pulawy 2010 54 495-499.

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