Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets

Open access


The aim of the study was to investigate gender-specific effects of a maternal treatment with dexamethasone (DEX) at multiple maximum therapeutic doses during the last 25 d of pregnancy on the bone tissue metabolism in neonatal piglets. BMD and BMC were measured. The geometry, histomorphometry, and mechanical properties of bones were determined. The assessment of selected hormones, cytokines, and amino acids was performed in 35-day-old piglets. DEX administered prenatally inhibited neonatal bone development, as well as bone mineral density, and geometrical and mechanical properties of bones. Neonatal GC-induced bone loss was linked with disturbed somatotropic axis function and decreased serum concentration of GH in male piglets and decreased concentration of IGF-1 in females. Prenatal DEX treatment led to an increase in the concentration of insulin and enhanced activity of bone specific alkaline phosphatase in piglets of both genders.

1. Céleste C., Ionescu M., Poole A.R., Laverty S.: Repeated intraarticular injections of triamcinolone acetonide alter cartilage matrix metabolism measured by biomarkers in synovial fluid. J Orthopc Res 2005, 23, 602-610.

2. Bertram C.E., Hanson M.A.: Prenatal programming of postnatal endocrine responses by glucocorticoids. Reproduction 2002, 124, 459-467.

3. Bloom S.L., Sheffield J.S., McIntire D.D., Leveno K.J.: Antenatal dexamethasone and decreased birth weight. Obstet Gynecol 2001, 97, 485-490.

4. Eastell R, Lambert H.: Diet and healthy bones. Calcif Tissue Int 2002, 70, 400-404.

5. Ekman S., Heinegård D.: Imunohistochemical localization of matrix proteins in the femoral joint cartilage of growing commercial pigs. Vet Pathol 1992, 29, 514-520.

6. Engle M.J., Kemnitz J.W., Rao T.J., Perelman R.H., Farrell P.M.: Effects of maternal dexamethasone therapy on fetal lung development in the rhesus monkey. Am J Perinatol 1996, 13, 399-407.

7. Ferretti J.L., Capozza R.F., Mondelo N., Zanchetta J.R.: Interrelationships between densitometric, geometric andmechanical properties of rat femora: inferences concerning mechanical regulation of bone modelling. J Bone Min Res 1993, 8, 395-1399.

8. Fowden A.L., Giussani D.A., Forhead A.J.: Endocrine and metabolic programming during intrauterine development. Early Human Develop 2005, 81, 723-734.

9. Henson F.M.D., Davies M.E., Jeffcott L.B.: Equine dyschondroplasia (Osteochondrosis) - histological findings and type VI collagen localization. Vet J 1997, 154, 53-62.

10. Holemans K., Aerts L., Van Assche F.A.: Fetal growth restriction and consequences for the offspring in animal models. J Soc Gynecol Invest 2003, 10, 392-399.

11. Holemans K., Aertis L., Van Assche A.: Fetal growth and long-term consequences in animal models of growth retardation. Eur J Obstet Gynecol 1998, 81, 149-156.

12. Huang K-C., Lue B-H., Yen R-F., Shen C.G., Ho S-R., Tai T-Y., Yang W-S.: Plasma adiponectin levels and metabolic factors in nondiabetic adolescents. Obesity Res 2004, 12, 119-124.

13. Jobe A.H., Wada N., Berry L.M., Ikegami M., Ervin M.: Single and repetitive maternal glucocorticoids exposures reduce fetal growth in sheep. Am J Obstet Gynecol 1998, 178, 880-885.

14. Katta J., Jin Z., Ingham E., Fisher J.: Biotribiology of articular cartilage - a review of the recent advances. Med Eng Physiol 2008, 30, 1349-1363.

15. Klemcke H.G., McGuire W.J., Christenson R.K.: Cortisol concentration in early porcine embryos. Life Sci 1999, 64, 1307-1312.

16. Laml T., Preyer O., Szulz-Lobmeyr I., Ruecklinger E., Hartmann B.W., Wagenbichler P.: Umbilical venous leptin concentration and gender in newborns. J Soc Gynecol Inves 2001, 8, 94-97.

17. Maccari S., Darnaudery M., Morley-Fletcher S., Zuena A.R., Cinque C., Van Reeth O.: Prenatal stress and longterm consequences: implications of glucocorticoids hormones. Neurosci Biobehav Rev 2003, 27, 119-127.

18. Marshall S., Garvey W.T., Traxinger R.R.: New insights into the metabolic regulation of insulin action and insulin resistance: role of glucose and amino acids. Exp Biol J 1991, 5, 3031-3036.

19. Miller S.C., Bowman B.M., Jee W.S.: Available animal models of osteopenia - small and large. Bone 1995, 17, 117S-123S.

20. Nagano M., Ozawa H., Suzuki H.: Prenatal dexamethasone exposure affects anxietylike behaviour and neuroendocrine systems in an age-dependent manner. Neurosci Res 2008, 60, 364-371.

21. Nathanielsz P.W., Thornburg K.L.: Fetal programming: from gene to functional systems -an overview. J Physiol 2003, 547, 3-4.

22. Newnham J.P., Moss T.J.: Antenatal glucocorticoids and growth: single versus multiple doses in animal and human studies. Semin Neonatol 2001, 6, 285-292.

23. O`Regan D., Welberg L.L.A.M., Holmes M.C., Seckl J.R.: Glucocorticoid programming of pituitary-adrenal function: mechanisms and physiological consequences. Semin Neonatol 2001, 6, 319-329.

24. Silha J.V., Krsek M., Skrha J.V., Sucharda P., Nyomba B.L., Murphy L.J.: Plasma resistin, adiponectin and leptin levels in lean and obese subjects: correlations with insulin resistance. Eur J Endocrinol 2003, 149, 331-335.

25. Simmons R.A., Templeton L.J., Gertz S.J.: Intrauterine growth retardation leads to the development of type 2 diabetes in the rat. Diabetes 2001, 50, 2279-2286.

26. Seckl J.R.: Physiologic programming of the fetus. Clin Perinatol 1998, 25, 939-964.

27. Seckl J.R.: Glucocorticoid programming of the fetus; adult phenotypes and molecular mechanisms. Mol Cell Endocrinol 2001, 185, 61-71.

28. Sève B., Ponter A.A.: Nutrient-hormone signals regulating muscle protein turnover in pigs. Proc Nutr Soc 1997, 56, 565-580.

29. Smink J.J., Buchholz I.M., Hamers N., van Tilburg C.M., Christis C., Sakkers R.J., de Meer K., van Buul- Offers S.C., Koedam J.A.: Short-term glucocorticoid treatment of piglets causes changes in growth plate morphology and angiogenesis. OsteoArtr Cart 2003, 11, 864-871.

30. Smith N.H., Ozanne S.E.: Intrauterine origins of metabolic disease. Rev Gynaecol Perinatal Pract 2006, 6, 211-217.

31. Śliwa E., Dobrowolski P.: Perinatal programing of skeletal system. J Pre-Clin Clin Res 2007, 1, 111-117.

32. Śliwa E., Dobrowolski P., Piersiak T.: Bone development of suckling piglets after prenatal, neonatal or perinatal treatment with dexamethasone. J Anim Physiol Anim Nutr 2010, 94, 293-306.

33. Śliwa E., Radzki R.P., Puzio I.: Osteochondrosis and tibial dyschondroplasy in chickens, pigs and foals. Medycyna Wet 1996, 52, 156-159.

34. Śliwa E., Tatara M.R., Nowakowski H., Pierzynowski S.G., Studziński T.: Effect of maternal dexamethsone and α-ketoglutarate administration on skeletal development during the last three weeks of prenatal life in pigs. J Matern Fetal Neonatal Med 2006, 19, 489-493.

35. Śliwa E., Kowalik S., Tatara M.R., Studziński T.: Effect of maternal administration of dexamethasone during last weeks of pregnancy on foetal skeletal system in pigs. Bull Vet Inst Pulawy 2004, 48, 449-452.

36. Weiler H., Wang Z., Atkinson S.: Lean body mass and protein and energy utilization for growth are lower in dexamethasone-treated piglets. Biol Neonate 1997, 71, 53-59.

37. Weiler H.A., Kovacs H., Murdock C., Adolphe J., Fitzpatrick-Wong S.: Leptin predicts bone and fat mass after accounting for the effects of diet and glucocorticoid treatment in piglets. Exp Biol Med 2002, 227, 639-644.

38. Yokota T., Oritani K., Takahashi I., Ishikawa J., Matsuyama A., Ouchi N., Kihara S., Funahashi T., Tenner A.J., Tomiyama Y., Matsuzawa Y.: Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages. Blood 2000, 96, 1723-1732.

Bulletin of the Veterinary Institute in Pulawy

The Journal of National Veterinary Research Institute in Pulawy

Journal Information

IMPACT FACTOR 2017: 0.811
5-year IMPACT FACTOR: 0.973

CiteScore 2017: 0.68

SCImago Journal Rank (SJR) 2017: 0.287
Source Normalized Impact per Paper (SNIP) 2017: 0.484

Cited By


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 204 187 15
PDF Downloads 63 56 4