The KHV free carp were experimentally infected with CyHV-3 suspension by immersion at temperatures of 18ºC±1 and 25ºC±1. Skin, brain, gills, liver, spleen, kidneys, and leukocytes were investigated for the presence of KHV DNA, and sera for the presence of antibodies against the virus. Similar investigations were performed with non-infected carp, designed as control groups. The results of KHV DNA detection in the infected carp kept at 25ºC ±1 were compared with the results of the virus isolation onto common carp brain cells using leukocyte co-cultivation method and results of specific antibody detection. The appearance of specific antibodies at various time intervals after detecting the clinical symptoms of KHVD in naturally infected carp was additionally studied. At 18°C water temperature, KHV DNA was not detected up to the 6th d post infection (dpi). Then it was found in samples from skin, gill, liver, spleen, and kidneys and much later from the brain. At 25°C water temperature, KHV DNA was already detected on the 3rd dpi in samples from skin and gills, and from the 5th dpi it was found in all examined internal organs, except the brain where it was found at 6th dpi. At 25°C, isolations of CyHV-3 have succeeded between the 7th and 11th dpi. The first specific antibodies were found no sooner than on the 21st dpi. The serological examination performed in naturally infected carp in 15 carp pond farms showed that first specific antiviral antibodies can be detected 2 weeks after the appearance of clinical symptoms of KHVD.
If the inline PDF is not rendering correctly, you can download the PDF file here.
1. Antychowicz J. Reichert M. Matras M.: Występowanie infekcji herpeswirusa karpia na świecie - zwalczanie tej choroby. Ochrona zdrowia ryb - aktualne problemy. Editor IRS Olsztyn Poland 2004.
2. Antychowicz J. Reichert M. Matras M. Bergmann S.M. Haenen O.: Epidemiology pathogenicity and molecular biology of koi herpesvirus isolated in Poland. Bull Vet Inst Pulawy 2005 49 367-373.
3. Bergmann S. M. Schütze H. Fischer U. Fichtner D. Riechardt M. Meyer K. Schrudde D. Kempter J.: Detection of koi herpes virus (KHV) genome in apparently healthy fish. Bull Eur Assoc Fish Pathol 2009 29 145-152
4. Eide K.E. Miller-Morgan T. Heide J.R. Kent M.L. Bildfell R. Lapatra S. Watson G. Jin L.: Investigation of koi herpesvirus (KHV) latency in koi. J Virol. 2011 10 4954-4962.
5. Gilad O. Yun S. Andree K.B. Adkison M.A. Zlotkin A. Bercovier H. Eldar A. Hedrick R.P.: Initial characteristics of koi herpesvirus and development a polymerase chain reaction assay to detect the virus in Cyprinus carpio koi. Dis Aquat Organ 2002 48 101-108.
6. Haenen O.L.M. Way K. Bergmann S.M. Ariel E.: The emergence of koi herpesvirus and its significance to European aquaculture. Bull Eur Assoc Fish Pathol 2004 24 293-307.
8. Ronen A. Perelberg A. Abramovitz J. Hutoran M. Tinman S. Bejerano I. Steinitz M. Kotler M.: Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio. Vaccine 2003 21 4625-4743.
9. St. Hilarie S. Beevers N. Way K. Le Duff R.M. Martin P. Joiner C.: Reactivation of koi carp herpesvirus infections in common carp Cyprinus carpio. Dis Aquat Organisms 2005 67 15-23.
10. St. Hilarie S. Beevers N. Joiner C. Wood G. Dunn P. Way K.: Development of a serological tool for koi herpesvirus surveillance. Proceedings of EAFP 13th International Conference. Grado Italy 2007.