Analysis of trichome morphology and density in Salvia nemorosa L. (Lamiaceae) of Iran

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Salvia nemorosa L. is an aromatic herb distributed widely in various regions of Iran, and is used in traditional and modern medicine. In the present study, leaf trichome densities and also type were investigated in six populations of the species, because the effective substances of this species exist in essential oil of glandular trichomes. For this, the mature intact leaves of each population were fixed in FAA solution, and then transverse hand sections were double-stained and studied using light microscopy. Leaf indumentum in all of the populations was dense pilose, with the exception of Tehran population that had loose pilose. Thirteen kinds of trichomes were identified, the main of which were peltate and capitate. PCA-biplot showed that each of these populations had prominent trichome trait(s). Furthermore, significant positive/negative correlations were found between some trichome types with main ecological factors of habitat. The studied populations clustered separately in the UPGMA tree, moreover, PCA and also PCO plots produced similar results. Our findings confirmed that ecological parameters of a habitat have strong effects on trichome morphology and density.

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  • Albayrak S. Aksoy A. 2013: Essential oil composition and in vitro antioxidant and antimicrobial activities of Thymus cappadocicus Boiss. – Journal of Food Processing and Preservation 37(5): 605–614.

  • Ascensão L. Marques N. Pais M.S. 1995: Glandu lar trichomes on vegetative and reproductive organs of Leonotis leonurus (Lamiaceae). – Annals of Botany 75: 619–626.

  • Ascensão L. Mota L. De Castro M.M. 1999: Glandular trichomes of the leaves and flowers of Plectranthus ornatus: morphology distribution and histochemistry. – Annals of Botany 84: 434 –447.

  • Askary M. Talebi S.M. Amini F. Dousti Balout Bangan A. 2016: Effect of NaCl and iron oxide nanoparticles on Mentha piperita essential oil composition. – Environmental and Experimental Biology 14: 27–32.

  • Bahadori M.B. Asghari B. Dinparast L. Zengin G. Sarikurkcu C. Abbas-Mohammadi M. Bahadori S. 2017: Salvia nemorosa L.: A novel source of bioactive agents with functional connections. – LWT Food Science and Technology 75: 42–50.

  • Bahadori M.B. Valizadeh H. Farimani M.M. 2016: Chemical composition and antimicrobial activity of the volatile oil of Salvia santolinifolia Boiss. From Southeast of Iran. – Pharmaceutical Sciences 22(1): 42–48.

  • Bercu R. Negrean G. Broască L. 2012: Leaf anatomical study of taxons Salvia nemorosa subsp. tesquicolaSalvia nutans and Salvia × sobrogensis from Dobrudja. – Botanica Serbica 36: 103–109.

  • Cano-Santana Z. Oyama K. 1992: Variation in leaf trichomes and nutrients of Wigandia urens (Hydrophyllaceae) and its implications for herbivory. – Oecologia 92: 405–409.

  • Coisin M. Burzo I. Ştefan M. Rosenhech E. Zamfirache M.M. 2012: Chemical composition and antibacterial activity of essential oils of three Salvia species widespread in eastern Romania. – Scientific Annals of Alexandru Ioan Cuza University of Iasi. New Series Section 2. Vegetal Biology 58(1): 51–58.

  • Ehleringer P. 1984: Ecology and ecophysíology of leaf pubescence in North American desert plants. – In: Rodriguez E. Healy P.L. Mehta L. (eds) Biology and chemistry of plant trichomes: 113–132. – New York.

  • Ehleringer J.R. Bjorkman O. Mooney H.A. 1976: Leaf pubescence: effects on absorbance and photosynthesis in a desert shrub. – Science 192: 376–377.

  • Eiji S. Salmaki Y. 2016: Evolution of trichomes and its systematic significance in Salvia (Mentheae; Nepetoideae; Lamiaceae). – Botanical Journal of the Linnean Society 180(2): 241–257.

  • Esau K. 1965: Plant Anatomy. – New York.

  • Farimani M.M. Bahadori M.B. Koulaei S.A. Salehi P. Nejad S. Reza E.H. Hamburger K. M. 2015: New ursane triterpenoids from Salvia urmiensis Bunge: Absolute configuration and antiproliferative activity. – Fitoterapia 106: 1–6.

  • Feijó E.V.R.S. Oliveira R.A. Costa L.C.B. 2014: Light affects Varronia curassavica essential oil yield by increasing trichomes frequency. – Revista Brasileira de Farmacognosia 24(5): 516–523.

  • Fu Q.S. Yang R.C. Wang H.S. Zhao B. Zhou C. L. Ren S.X. Guo Y.D. 2013: Leaf morphological and ultrastructural performance of eggplant (Solanum melongena L.) in response to water stress. – Photosynthetica 51: 109–114.

  • Glover B.J. 2000: Differentiation in plant epidermal cells. – Journal of Experimental Botany 51: 497–505.

  • Gonzales W.L. Negrittoa M.A. Suarez L.H. Gianoli E. 2008: Induction of glandular and nonglandular trichomes by damage in leaves of Madia sativa under contrasting water regimes. – Acta Oecologica 33: 128–132.

  • Hayat M.Q. Ashraf M. Khan M.A. Yasmin G. Shaheen N. Jabeen S. 2009: Diversity of foliar trichomes and their systematic implications in the genus Artemisia (Asteraceae). – International Journal of Agriculture and Biology 11: 542–546.

  • Jamzad Z. 2012: Lamiaceae. – In: Assadi M. Maassoumi A. Mozaffarian V. (eds) Flora of Iran 76: 564–670 Tehran.

  • Karousou R. Bosabalidis A.M. Kokkini S. 1992: Sideritis syriaca ssp. syriaca: Glandular trichome structure and development in relation to systematics. – Nordic Journal of Botany 12: 31–37.

  • Kelsey R.G. Reynolds G.W. Rodriguez E. 1984: The chemistry of biologically active constituents secreted and stored in plant glandular trichomes. – In: Rodriguez E. Healey P.L. Mehta I. (eds) Biology and Chemistry of Plant Trichomes. – New York.

  • Lemberkovics M. E. Marczal G. 2003: Study of plant anatomical characteristics and essential oil composition of Hungarian Salvia species. – Acta Horticulture 597: 143–148.

  • Maleci L.B. Servettaz O. 1991: Morphology and distribution of trichomes in Italian species of Teucrium sect. Chamaedrys (Labiatae) Da taxonomical evaluation. – Plant Systematics and Evolution 174: 83–91.

  • Meshkatalsadat M.H. Norani M. 2015: Chemical composition of the essential oil of Salvia nemorosa using gas chromatography mass spectroscopy. – Journal of Novel Applied Sciences 4(2): 140–142.

  • Naydenova G.K. Georgiev G.I. 2013: Physiological function of non-glandular trichomes in red clover (Trifolium pratense L.). – Journal of Agricultural Sciences 58(3): 217–222.

  • Nikolic M. Glamoclija J. Ferreira I.C.F.R. Calhelha R.C. Fernandes A. Markovic T. Markovi D. Giweli A. Sokovic M. 2014: Chemical composition antimicrobial antioxidant and antitumor activity of Thymus serpyllum L. Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. – Industrial Crops and Products 52: 183–190.

  • Peter J. Shanower T. 1998: Plant glandular trichomes chemical factories with many potential uses. – Resonance 3(3): 41–45.

  • Pérez-Estrada L.B. Cano-Santana Z. Oyama K. 2000: Variation in leaf trichomes of Wigandia urens: environmental factors and physiological consequences. – Tree Physiology 20: 629–632.

  • Podani J. 2000: Introduction to the Exploration of Multivariate Biological Data. – Leiden.

  • Serrato-Valenti B. A. Cornara L. Ciarallo G. 1997: Structural and histochemical investigation of the glandular trichomes of Salvia aurea L. leaves and chemical analysis of the essential oil. – Annals of Botany 79: 329–336.

  • Skelton R.P. Midgley J.J. Nyaga J.M. Johnson S. D. Cramer M. D. 2012: Is leaf pubescence of Cape Proteaceae a xeromorphic or radiation protective trait? – Australian Journal of Botany 60: 104–113.

  • Sletvold N. Ågren J. 2012: Variation in tolerance to drought among Scandinavian populations of Arabidopsis lyrata. – Evolutionary Ecology 26: 559–77.

  • Seyedi Z. Salmaki Y. 2015: Trichome morphology and its significance in the systematics of Phlomoides (Lamiaceae; Lamioideae; Phlomideae). – Flora 213: 40–48.

  • Talebi S.M. Rezakhanlou A. Salahi Isfahani G. 2012: Trichomes plasticity in Ziziphora tenuior L. (Labiatae) in Iran: An ecological review. – Annals of Biological Research 3(1): 668–672.

  • Talebi S.M. Shayestehfar A.R. 2014: Infraspecific trichomes variations in Acinos graveolens (M.B.) Link. – Annals of Biological Sciences 2(2): 51–57.

  • Telepova M.N. Budantzev A.L. Shavarda A.L. 1992: Étude comparative de la sécreétion des terpénes par les éléments glandulaires foliaireschez différentes espéices du genre Dracocephalum L. (Labiatae). – Bulletin de la Société Botanique de France 139: 247–264.

  • Vogelmann T.C. 1993: Plant-tissue optics. – Annual Review of Plant Physiology and Plant Molecular Biology 44: 231–251.

  • Werker E. Ravid U. Putievsky E. 1985a: Structure of glandular hairs and identification of the main components of their secreted material in some species of the Labiatae. – Israel Journal of Botany 34: 31–45.

  • Werker E. Ravid U. Putievsky E. 1985b: Glandular hairs and their secretion in the vegetative and reproductive organs of Salvia sclarea and Salvia dominica. – Israel Journal of Botany 34: 239–252.

  • Zengin G. Sarikurkcu C. Aktumsek A. Ceylon R. 2014: Sideritis galatica Bornm.: A source of multifunctional agents for the management of oxidative damage Alzheimer ’s and diabetes mellitus. – Journal of Functional Foods 11: 538–547.

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