Sonication (Ultrasound) Affects In Vitro Growth of Hybrid Cymbidium

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Abstract

The use of ultrasound or sonication has been shown to stimulate growth and development of several plant species. No study exists on the impact of sound on Cymbidium growth in vitro. Using sonication at 60 Hz for several time periods, the response on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined, as was the response on in vitro plant organogenesis. Sonication for 5 or 10 min stimulated neo-PLB formation significantly more than plant growth regulator (PGR)-free TC medium without sonication (negative control) and more than 1, 20 or 45 min sonication, but significantly less than control TC medium containing PGRs NAA and Kin (positive control) after 60 days in culture. Sonication, when applied to PLBs, did not influence most plantlet-related growth parameters. Flow cytometric analyses registered an increase in endoreduplication in sonicated PLB tissues. Sonication at 60 Hz has a PLB-promoting effect, but is not as effective as PGRs.

Chen B., Huang J., Wang J., Huang L., 2008: Ultrasound effects on the antioxidative defense systems of Porphyridium cruentum. - Colloids and Surfaces B: Biointerfaces, 61: 88-92.

Hossain M.M., Kant R., van P.T., Winarto B., Zeng S-J., Teixeira Da Silva J.A., 2013: The application of biotechnology to orchids. - Critical Reviews in Plant Sciences, 32(2): 69-139.

Liu Y-Y., Yoshikoshi A., Wang B-c., Sakanishi A., 2003 a: Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nippon-bare callus. - Colloids and Surfaces B: Biointerfaces, 27: 287-293.

Liu Y-Y., Takatsuki H., Yoshikoshi A., Wang B-C., Sakanishi A., 2003b: Effects of ultrasound on the growth and vacuolar H+-ATPase activity of Aloe arborescens callus cells. - Colloids and Surfaces B: Biointerfaces, 32: 105-116.

Mason T.J., 2007: Developments in ultrasound -non-medical. - Progress in Biophysics & Molecular Biology, 93: 166-175.

Mishiba K., Mii K., 2000: Polysomaty analysis in diploid and tetraploid Portulaca grandiflora. -Plant Science, 156: 213-219.

Nyborg W.L., 1982: Ultrasonic microstreaming and related phenomena. - The British Journal of Cancer Supplement, 5: 156-160.

Rokhina E.v., Lens P., virkutyte J., 2009: Low-frequency ultrasound in biotechnology: state of the art. - Trends in Biotechnology, 27: 298-306.

Shao H., Li B., Wang B., Tang K., Liang Y., 2008: A study of differentially expressed gene screening of Chrysanthemum plants under sound stress. -Comptes Rendus Biologies, 331: 329-333.

Sharma S., Shahzad A., Teixeira da Silva J.A., 2013: Synseed technology - a complete synthesis. - Biotechnology Advances, 31(2): 186-207.

Teixeira da Silva J.A., 2005: Simple multiplication and effective genetic transformation (4 methods) of in vitro-grown tobacco by stem thin cell layers. - Plant Science, 169(6): 1046-1058.

Teixeira da Silva J.A., 2012a: Production of synseed for hybrid Cymbidium using protocorm-like bodies. - Journal of Fruit and Ornamental Plant Research, 20(2): 135-146.

Teixeira da Silva J.A., 2012b: New basal media for protocorm-like body and callus induction of hybrid Cymbidium. - Journal of Fruit and Ornamental Plant Research, 20(2): 127-133.

Teixeira da Silva J.A., 2013 a: Orchids: advances in tissue culture, genetics, phytochemistry and transgenic biotechnology. - Floriculture and Ornamental Biotechnology, 7(1): 1-52.

Teixeira da Silva J.A., 2013b: The role of thin cell layers in regeneration and transformation in orchids. - Plant Cell, Tissue and Organ Culture, 113(2): 149-161.

Teixeira da Silva J.A., Fukai S., 2003: Gene introduction method affects the shoot regeneration of in vitro and greenhouse-grown chrysanthemum (Dendranthema x grandiflora (Ramat.) Kita-mura). - African Journal of Biotechnology, 2(5): 114-123.

Teixeira da Silva J.A., Tanaka M., 2006: Embryo-genic callus, PLB and TCL paths to regeneration in hybrid Cymbidium (Orchidaceae). - Journal of Plant Growth Regulation, 25(3): 203-210

Teixeira da Silva J.A., DobrAnszki J., 2013: How timing of sampling can affect the outcome of the quantitative assessment of plant organogenesis. -Scientia Horticulturae, 159: 59-66.

Teixeira da Silva J.A., Dobranszki J., 2014: Sonica-tion and ultrasound: impact on plant growth and development. - Plant Cell, Tissue and Organ Culture, 117: 131-143.

Teixeira da Silva J.A., Yam T., Fukai S., Nayak N., Tanaka M., 2005: Establishment of optimum nutrient media for in vitro propagation of Cymbidium Sw. (Orchidaceae) using protocorm-like body segments. - Propagation of Ornamental Plants, 5(3): 129-136.

Teixeira da Silva J.A., Chan M-T., Sanjaya, Chai M-L., Tanaka M., 2006a: Priming abiotic factors for optimal hybrid Cymbidium (Orchida-ceae) PLB and callus induction, plantlet formation, and their subsequent cytogenetic stability analysis. - Scientia Horticulturae, 109(4): 368378.

Teixeira da Silva J.A., Singh N., Tanaka M., 2006b: Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plantlets. - Plant Cell, Tissue and Organ Culture, 84(2): 135-144.

Teixeira da Silva J.A., Norikane A., Tanaka M., 2007: Cymbidium: successful in vitro growth and subsequent acclimatization. - Acta Horticulturae, 748: 207-214.

Teixeira da Silva J.A., DobrAnszki J., Ross S., 2013: Phloroglucinol in plant tissue culture. - In Vitro Cellular and Developmental Biology - Plant, 49(1): 1-16.

Trick H.N., Finer J.J., 1997: SAAT: sonication-as-sisted Agrobacterium-mediated transformation. -Transgenic Research, 6: 329-336.

vacin E., Went F.W., 1949: Some pH changes in nutrient solutions. - Botanical Gazette, 110: 605-613.

Wang B., Shao J., Li B., Lian J., Duan C., 2004: Soundwave stimulation triggers the content change of the endogenous hormone of the Chrysanthemum mature callus. - Colloids and Surfaces B: Biointerfaces, 37: 107-112.

Wang B., zhao H., Wang x., Duan z., Wang D., Sakanishi A., 2002: Influence of sound stimulation on plasma membrane H+-ATPase activity. - Colloids and Surfaces B: Biointerfaces, 25: 183-188.

Wang B.C., Yoshikoshi A., Sakanishi A., 1998: Carrot cell growth in a stimulated ultrasonic environment. - Colloids and Surfaces B: Biointerfaces, 12: 89-95.

Wang B.C., Zhou J., Wang Y.C., Zhu L.C., Teixeira da silva J.A., 2006: Physical stress and plant growth. - In: Teixeira da silva J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, 2: 68-85. - Isleworth.

Wang X., Wang B., Jia y., Duan C., sakanishi A., 2003: Effect of sound wave on the synthesis of nucleic acid and protein in chrysanthemum. - Colloids and Surfaces B: Biointerfaces, 29: 99-102.

Wang Y., Luo J.P., Wu H.Q., Jin H., 2009: Conversion of protocorm-like bodies of Dendrobium huoshanense to shoots: the role of polyamines in relation to the ratio of total cytokinins and indole-3-acetic acid. - Journal of Plant Physiology, 166: 2013-2022.

Wei M., Yang C-Y., Wei S-H., 2012: Enhancement of the differentiation of protocorm-like bodies of Dendrobium officinale to shoots by ultrasound treatment. - Journal of Plant Physiology, 169(8): 770-774.

Zheng Q., Zheng Y., Wang G., Guo W., Zhang Z., 2011: Sonication-assisted Agrobacterium-medi-ated transformation of chalcone synthase (CHS) gene to spring Dendrobium cultivar 'Sanya'. -African Journal of Biotechnology, 10(56): 11832-11838.

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CiteScore 2017: 0.50

SCImago Journal Rank (SJR) 2017: 0.186
Source Normalized Impact per Paper (SNIP) 2017: 0.130

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