Response of Hybrid Cymbidium (Orchidaceae) Protocorm-Like Bodies to 26 Plant Growth Regulators

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Abstract

Teixeira da Silva J.A., 2014: Response of hybrid Cymbidium (Orchidaceae) protocorm-like bodies to 26 plant growth regulators [Cymbidium (Orchidaceae) hibrido į protokormą panašių kūnelių reakcija į 26 augalų augimo reguliatorius]. - Bot. Lith., 20(1): 3-13.

Plant growth regulators (PGRs) are one of the most effective means of controlling plant organogenesis in vitro. Hybrid orchid production relies on effective protocols to maximize clonal shoot output. This is best achieved when protocormlike bodies (PLBs) are propagated. In a bid to deepen orchidologists’ understanding of basic responses of Cymbidium to PGRs, this study aimed to establish the organogenic response of hybrid Cymbidium Twilight Moon ‘Day Light’ half-PLBs or PLB thin cell layers (TCLs) to a single application of PGRs (6 auxins; 7 cytokinins; 3 alternative PGRs), 3 herbicides or 7 growth inhibitors/retardants at 4 concentrations (1, 2, 4 or 8 mg·1-1) as well as a control (0 mg·1-1), both in the light and in the dark. The control (PGR-containing TC medium) performed best, but all auxins and growth inhibitors and retardants were toxic to neo-PLB formation, resulting in 100% death. A synthetic auxin (BSAA), a cytokinin (4-CPPU) and two herbicides (dicamba and picloram) were equally toxic. No auxins, TIBA, GA3 or SA induced any organogenic response. 1 or 2 mg·1-1 2,4-D or 1 mg·1-1 TDZ induced embryogenic callus, but 2-8 mg·1-1 2,4-D resulted in abnormal shoots. TDZ induced direct multiple shoots. Only five remaining cytokinins (Ads, BA, Kin, ZR, 2iP) could form neo-PLBs, but always significantly less than the controls, independent of the explant used (half- PLBs or tTCLs) and light conditions (light vs darkness). These five cytokinins could be useful for neo-PLB induction of other Cymbidium hybrids. A new concept, the average cumulative value or ACV, is introduced

Santrauka

Augalų augimo reguliatoriai (AAR) yra viena iš veiksmingiausių augalų organogenezės in vitro valdymo priemonių. Hibridinių orchidėjų dauginimas vykdomas efektyviais metodais, besiremiančiais klonuotų ūglių išeigos didinimu. Tai geriausiai pasiekiama, kai yra dauginami į protokormus panašūs kūneliai (PPK). Siekiant pagilinti orchidologistų žinias apie pagrindines Cymbidium reakcijas į augimo reguliatorius, šio tyrimo tikslas buvo nustatyti Cymbidium hibrido Twilight Moon ‘Day Light’ organogenezinį atsaką, plonus ląstelių sluoksnius (PLS) iš PPK ar pusiau PPK paveikus augimo reguliatoriais (9 auksinais; 7 citokininais; 3 alternatyviais AAR) arba 5 koncentracijų (0, 1, 2, 4 arba 8 mg/l) 7 augimo inhibitoriais / retardantais tiek šviesoje, tiek ir tamsoje. Augimas buvo geriausias kontrolinėje terpėje (Tekseiros Cymbidium TC terpė su AAR), tačiau visi auksinai, augimo inhibitoriai ir retardantai buvo toksiški naujų PKK formavimuisi ir sukėlė 100 % žūtį. Sintetinis auksinas (BSAA), citokininas (4-CPPU) ir du herbicidai (dikamba ir pikloramas) buvo vienodai toksiški. Auksinai, TIBR, GR3 ar SR nesukėlė jokio organogenezinio atsakymo. Retardantas 2,4-D (nuo 1 iki 2 mg/l) arba 1 mg/l TDZ sukėlė kaliaus embriogenezę, o paveikus 2-8 mg/l 2,4-D atsirado nenormalių ūglių. TDZ sukėlė tiesius daugybinius ūglius. Tik likę 5 citokininai (Ads, BA, Kinetinas, ZR, 2iP) sugebėjo suformuoti neo-PPK, bet visada mažesnį skaičių nei kontrolinėje terpėje, tai nepriklausė nuo panaudoto eksplanto (pusės PPK ar PLS) ir apšvietimo sąlygų (šviesos ar tamsos). Šie 5 citokininai gali būti vertingi kitų Cymbidium hibridų neo-PPK indukcijai. Taip pat aptariama nauja vidutinių verčių (VV) įvertinimo koncepcija.

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