Novel Factors Affecting Shoot Culture of Chrysanthemum (Dendranthema × Grandiflora)

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Teixeira da Silva J.A., 2014: Novel factors affecting shoot culture of chrysanthemum (Dendranthema × grandiflora) [Alternatyvių standiklių, skystų terpės priedų, CO2 sodrinimo ir kitų faktorių įtaka chrizantemų (Dendranthema × grandiflora (Ramat.) Kitamura) ūglių kultūrų auginimui]. - Bot. Lith., 20(1): 27-40.

Chrysanthemum (Dendranthema × grandiflora (Ramat.) Kitamura) continues to be one of the most important ornamental plants in the world. Although the tissue culture of chrysanthemum has been widely explored, several unexplored topics remain, and, in developing countries, there is always the constant search for reducing the cost of raising tissue cultured plants. In this study, by focusing on a leading market cultivar in Japan, ‘Shuhouno- chikara’, alternatives to agar (as the gelling agent) and sucrose (as the carbon source) for chrysanthemum tissue culture were sought. Both Gellan gum and agar resulted in greater shoot and root production than all other gelling agents tested, including Bacto agar, phytagel, oatmeal agar, potato dextrose agar, barley starch and corn starch. All of the alternative liquid-based medium additives tested (low and full fat milk, Coca-cola ®, coffee, Japanese green, Oolong and Darjeeling teas) negatively impacted plant growth, stunted roots and decreased chlorophyll content (SPAD value) of leaves. There was no difference between plants grown on medium with refined sucrose or table sugar, although poor growth was observed when stevia (Stevia rebaudiana) extract was used. Photoautotrophic micropropagation increased significantly the shoot mass relative to control plants, even when the density of plants was doubled. Aeration improved plantlet growth. The tetrazolium test was a simple, but effective essay to see the intensity and strength of root growth in different basal media. MDH activity decreased in the root+shoot extract of plants grown on most alternative media, but remained high on TCSGM (Teixeira’s chrysanthemum shoot growth medium), Gellan gum, aerated and CO2-enriched cultures. A similar trend was observed for deaminating GDH, while an opposite trend was observed for aminating GDH activity. These experiments indicate that tissue culture research for chrysanthemum still provides a rich field for exploration with interesting and valuable results

Anissi J., El Hassouni M., Ouardaoui A., Sendide K., 2014: A comparative study of the antioxidant scavenging activity of green tea, black tea and coffee extracts: a kinetic approach. - Food Chemistry, 150: 438-447.

Babb ar S.B., Jain N., 1998: ‘Isubgol’ as an alternative gelling agent in plant tissue culture. - Plant Cell Reports, 17(4): 318-322.

Babb ar S.B., Jain R., Walia N., 2005: Guar gum as a gelling agent for plant tissue culture media. - In Vitro Cellular and Developmental Biology - Plant, 41(3): 258-261.

Beruto M., Curir P., 2006: Effects of agar and gel characteristics on micropropagation: Ranunculus asiaticus, a case study. - In: Teixeira da Silva J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology. Advances and Topical Issues, 2: 277-284. - Isleworth. bhattacharya p., dey s., bhattacharya b.c., 1994: Use of low-cost gelling agents and support matrices for industrial scale plant tissue culture. - Plant Cell, Tissue and Organ Culture, 37(1): 15-23.

Bonga J.M., Aderkas P.V., 1992: In Vitro Culture of Trees. - Dordrecht.

Calle F., Martin M., Sabater B., 1986: Cytoplasmic and mitochondrial localization of the glutamate dehydrogenase induced by senescence in barley (Hordeum vulgare). - Physiologia Plantarum, 66(3): 451-456.

Cameron S.I., 2008: Plant tissue culture gelling agents and supports: history, development and function. - In: Teixeira da Silva J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology. Advances and Topical Issues, 5: 171-190. - Isleworth.

Casanova E., Moysset L., Trillas M.I., 2008: Effect of agar concentration and vessel closure on the organogenesis and hyperhydricity of adventitious carnation shoots. - Biologia Plantarum, 52(1): 1-8.

Chauvin J-E., Marhadour S., Cohat J., Le Nard M., 1999: Effects of gelling agents on in vitro regeneration and kanamycin efficiency as a selective agent in plant transformation procedures. - Plant Cell, Tissue and Organ Culture, 58(3): 213-217.

Coste S., Baraloto C., Leroy C., Marcon E., Enaud a., Richardson A.D., Rogg y J-C., Schimann H., Uddling J., Hérault B., 2010: Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana. - Annals of Forest Science 67(6): 607.

Dobránszki J., Magyar-Tábori K., Tomb ácz E., 2011: Comparison of the rheological and diffusion properties of some gelling agents and blends and their effects on shoot multiplication. - Plant Biotechnology Reports, 5(4): 345-352.

Food And Water Watch, 2010: Consolidation and price manipulation in the dairy industry. http:// Competition-web.pdf [accessed 14.03. 2013].

George E.F., 1996: Plant Propagation by Tissue Culture. Part 2. In Practice. - Edington.

Gour V.S., Kant T., 2011: Efficacy of low cost gelling agents and carbon source alternatives during in vitro rooting of Balanites aegyptiaca. - Tree and Forestry Science and Biotechnology, 5 (Special Issue 1): 58-60.

Gulati A., Jaiwal P.K., 1996: Effect of NaCl on nitrate reductase, glutamate dehydrogenase and glutamate in Vigna radiata calli. - Biologia Plantarum, 38(2): 177-183.

Hahn E., Bae J., Lee J.H., Beom Y., 1998: Growth and leaf-surface characteristics of chrysanthemum plantlets between hydroponic and microponic system. - Journal of the Korean Society for Horticultural Science, 39(6): 838-842.

Hahn E-J., Paek K-Y., 2005: Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems. - Plant Cell, Tissue and Organ Culture, 81(3): 301-306.

Hare P.D., Cress W.A., van Staden J., 1998: Dissecting the roles of osmolyte accumulation during stress. - Plant Cell & Environment, 21(6): 535-553.

Henderson W.E., Kinnersley A.M., 1988: Corn starch as an alternative gelling agent for plant tissue culture. - Plant Cell, Tissue and Organ Culture, 15(1): 17-22.

Ichihashi S., Hiraiwa H., 1996: Effect of solidifier, coconut water, and carbohydrate source on growth of embryogenic callus in Phalaenopsis and allied genera. - Journal of the Orchid Society of India, 10: 81-88.

Ishii Y., Takamura T., Goi M., Tanaka M., 1998: Callus induction and somatic embryogenesis of Phalaenopsis. - Plant Cell Reports, 17: 446-450.

Ivanova M., van Staden J., 2011: Influence of gelling agent and cytokinins on the control of hyperhidricity in Aloe polyphylla. - Plant Cell, Tissue and Organ Culture, 104(1): 13-21.

Jain R., Babb ar S.B., 2005: Guar gum and isubgol as cost-effective alternative gelling agents for in vitro multiplication of an orchid, Dendrobium chrysotoxum. - Current Science, 88(2): 292-295.

Jain r., Babb ar S.B., 2006: Xanthan gum: an economical substitute of agar for in vitro multiplication of an orchid, Dendrobium chrysotoxum Lindl. - Current Science, 91(1): 27-28.

Jain a., Kantia A., Kothari S.L., 2001: De novo differentiation of shoot buds from leaf callus of Dianthus caryophyllus L. and control of hyperhydricity. - Scientia Horticulturae, 87(4): 319-326.

Jorge I.C., Mangolin C.A., Machado M.F., 1997: Malate dehydrogenase isoenzymes in long term callus culture of Cereus peruvianus (Cactaceae) exposed to sugar and temperature stress. - Biochemical Genetics, 35(5-6): 155-164.

Joshi p., Trivedi R., Purohit S.D., 2009: Micropropagation of Wrightia tomentosa: effect of gelling agents, carbon source and vessel type. - Indian Journal of Biotechnology, 8(1): 115-120. Kadoka M., Niimi Y., 2003: Effects of cytokinin types and their concentration on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. - Plant Cell, Tissue and Organ Culture, 72(3): 261-265.

Korir M.W., Wachira F.N., Wanyoko J.K., Ngure R .M., Khalid R., 2014: The fortification of tea with sweeteners and milk and its effect on in vitro antioxidant potential of tea product and glutathione levels in an animal model. - Food Chemistry, 145: 145-153.

Kumar R.G., Shah K., Dubey R.S., 2000: Salinity induced behavioural changes in malate dehydrogenase and glutamate dehydrogenase activities in rice seedlings of differing salt tolerance. - Plant Science, 156: 23-34.

Kurzbaum E., Kirzhner F., Armon R., 2010: A simple method for dehydrogenase activity visualization of intact plant roots grown in soilless culture using tetrazolium violet. - Plant Root, 4: 12-16.

Lauriere C., Daussant J., 1983: Identification of the ammonia-dependent isoenzyme of glutamate dehydrogenase as the form induced by senescence or darkness stress in the first leaf of wheat. - Physiologia Plantarum, 58(1): 89-92.

Maliro F.A.M., Lameck G., 2004: Potential of cassava flour as a gelling agent in media for plant tissue cultures. - African Journal of Biotechnology, 3(4): 244-247.

Mattioni C., Gabb rielli R., Vongronsveld J., Clijsters H., 1997: Nickel and cadmium toxicity and enzymatic activity in Ni tolerant and non-tolerant populations of Silene italica Pers. - Journal of Plant Physiology, 150(1-2): 173-177.

Meireles M.A.A., Wang G-M., Hao Z-B., Shima K., Teixeira da Silva J.A., 2006: Stevia (Stevia rebaudiana Bertoni): futuristic view of the sweeter side of life. - In: teixeira da silva j.a. (ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, 4: 416-425. - Isleworth.

Mitra A., Bhattacharya P.S., Dey S., Sawarkar S .K., Bhattacharya B.C., 1998: Photoautotrophic in vitro culture of chrysanthemum under CO2 enrichment. - Biotechnology Techniques, 12(4): 335-337.

Modi P., Sinha A., Kothari S.L., 2009: Reduction of hyperhydricity in micropropagated French marigold (Tagetes patula L.) plants by modified medium parameters. - Floriculture and Ornamental Biotechnology, 3: 40-45.

Morris E.R., Nishinari K., Rinaudo M., 2012: Gelation of gellan - a review. - Food Hydrocolloids, 28 (2): 373-411.

Murashige T., Skoog F., 1962: A revised medium for rapid growth and bioassay with tobacco tissue cultures. - Physiologia Plantarum, 15(3): 473-497.

Norikane A., Takamura T., Morokuma M., Tanaka M., 2010: In vitro growth and single-leaf photosynthetic response of Cymbidium plantlets to super- elevated CO2 under cold cathode fluorescent lamps. - Plant Cell Reports, 29(3): 273-283.

Norikane A., Teixeira da Silva J.A., Tanaka M., 2013: Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps (CCFLs) with super-elevated CO2 enrichment. - AoB Plants, 5: plt044.

Prakash S., 1993: Production of ginger and turmeric through tissue culture methods and investigations into making tissue culture propagation less expensive. - PhD thesis, Bangalore University, Bangalore.

Prakash S., Hoque M.I., Brinks T., 2004: Culture media and containers. - In: Low cost options for tissue culture technology in developing countries, FAO/IAEA Division of Nuclear Techniques in Food and Agriculture: 29-40. - Vienna.

Purohit S.D., Teixeira da Silva J.A., Habibi N., 2011: Current approaches for cheaper and better micropropagation technologies. - International Journal of Plant Developmental Biology, 5: 1-36.

Sadunishvili T., Kvesitadze E., Betsiashvili M., Kuprava N., Zaalishvil G., Kvesitadze G., 2009: Influence of hydrocarbons on plant cell ultrastructure and main metabolic enzymes. - World Academy of Science, Engineering and Technology, 57: 271-276.

Sánchez-rodríguez J., Martínez-Carrasco R., Pérez P., 1977: Photosynthetic electron transport and carbon-reduction cycle enzyme activities under long term drought stress in Casuarina equisetifolia forest. - Photosynthetic Research, 52(3): 255-262.

Scholten H.J., Pierik R.L.M., 1998: Agar as a gelling agent: chemical and physical analysis. - Plant Cell Reports, 17(3): 230-235. Shinoyama H., Anderson N., Furuta H., Mochizuki A ., Nomura Y., Singh RP., Datta S.K., Wang B-C., Teixeira da Silva J.A., 2006: Chrysanthemum biotechnology. - In: Teixeira da Silva J.A. (ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues: 140-163. - Isleworth.

Sorvari S., 1986: The effect of starch gelatinized nutrient media in barley anther cultures. - Annales Academiae Scientiarum Fennicae, 25: 127-133.

Tanaka M., 1992: Effect of different films used for film culture vessels on plantlet development of Phalaenopsis and Cymbidium. - Acta Horticulturae, 319: 225-230.

Teixeira da Silva J.A., 2003: Chrysanthemum: advances in tissue culture, postharvest technology, genetics and transgenic biotechnology. - Biotechnology Advances, 21(8): 715-766.

Teixeira da Silva J.A., 2004a: Ornamental chrysanthemums: improvement by biotechnology. - Plant Cell, Tissue and Organ Culture, 79(1): 1-18.

Teixeira da Silva J.A., 2004b: The effect of carbon source on the in vitro organogenesis of chrysanthemum thin cell layers. - Bragantia, 63(2): 165-177.

Teixeira da Silva J.A., 2005: Effective and comprehensive chrysanthemum (Dendranthema × grandiflora) regeneration and transformation protocols. - Biotechnology, 4(2): 94-107.

Teixeira da Silva J.A., 2014: Organogenesis from chrysanthemum (Dendranthema × grandiflora (Ramat.) Kitamura) petals (disc and ray florets) induced by plant growth regulators. - Asia-Pacific Journal of Molecular Biology and Biotechnology [in press].

Teixeira da Silva J.A., Giang D.T.T., Tanaka M., 2006: Novel photoautotrophic micropropagation of Spathiphyllum. - Photosynthetica, 44(1): 53-61.

Teixeira da Silva J.A., Tanaka M., 2009: Impact of gelling agent and alternative medium additives on hybrid Cymbidium protocorm-like body and callus formation. - Floriculture and Ornamental Biotechnology, 3: 56-58.

Teixeira da Silva J.A., Shinoyama H., Aida R., Matsushita Y., Raj S.K., Chen F., 2013: Chrysanthemum biotechnology: Quo vadis? - Critical Reviews in Plant Sciences, 32(1): 21-52.

Teixeira da Silva J.A., Tran Thanh Van k., Biondi S ., Nhut D.T., Altamura M.M., 2007a: Thin cell layers: developmental building blocks in ornamental biotechnology. - Floriculture and Ornamental Biotechnology, 1(1): 1-13.

Teixeira da Silva J.A., Giang D.T.T., Chan M-T., Sanjaya, Norikane A., Chai m-l., Chico-Ruíz J., Penna S., Granstrøm T., Tanaka M., 2007b: The influence of different carbon sources, photohetero-, photoautoand photomixotrophic conditions on protocorm-like body organogenesis and callus formation in thin cell layer culture of hybrid Cymbidium (Orchidaceae). - Orchid Science and Biotechnology, 1(1-2): 15-23.

Tsay H-S., Lee C-Y., Agrawal D.C., Basker S., 2006: Influence of ventilation closure, gelling agent and explant type on shoot bud proliferation and hyperhydricity in Scrophularia yoshimurae - a medicinal plant. - In Vitro Cellular and Developmental Biology - Plant, 42(5): 445-449.

Turner S.R., Singha S., 1990: Vitrification of crabapple, pear and gum on gellan gum-solidified culture medium. - Horticultural Science, 25(12): 1648-1650.

Van P.T., Teixeira da Silva J.A., Tanaka M., 2012a: How does choice of substrate and culture conditions affect the growth and development of Cymbidium cv. Green Planet ‘Energy Star’ protocorm-like bodies? - The European Journal of Horticultural Science, 77(5): 219-225.

Van P.T., Teixeira da Silva J.A., Ham L.H., Tanaka M., 2012b: Effects of permanent magnetic fields on in vitro growth of Cymbidium and Spathiphyllum shoots. - In Vitro Cellular and Developmental Biology - Plant, 48(2): 225-232.

Wang H-Y., He S-L., Tanaka M., Pham T.V., Teixeira da Silva J.A., 2012: Effect of IBA concentration, carbon source, substrate, and light source on root induction ability of tree peony (Paeonia suffruticosa Andr.) plantlets in vitro. - The European Journal of Horticultural Science, 77(3): 122-128.

Wojciechowski C.L., Kantrowitz E.R., 2002: Altering of the metal specificity of Escherichia coli alkaline phosphatase. - The Journal of Biological Chemistry, 277 (52): 50476-50481.

Yamaya T., Oaks A., 1987: Synthesis of glutamate by mitochondria - an anaplerotic function for glutamate dehydrogenase. - Physiologia Plantarum, 70(4): 749-756.

Zimm erman R.H., Bhardwaj S.V., Fordham I.M., 1995: Use of starch-gelled medium for tissue culture of some fruit crops. - Plant Cell, Tissue and Organ Culture, 43(3): 207-213.


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