Methods for Cyanotoxins detection

Open access

Abstract

Koreivienė J., Belous O., 2012: Methods for cyanotoxins detection [Cianotoksinų nustatymo metodai]. - Bot. Lith., 18(1): 58-65. Global occurrence and concern about microcystin contamination, the potential consequences of exposure to cyanobacterial toxins in recreational and drinking waters promoted the development of numerous methods to detect the toxin and their producers as well as identification and quantification of toxins. In current study we overview numerous methods that have been developed for the cyanotoxin analysis. We discuss advantages and shortages of their applications to solve different questions.

AlLayl K. J., Poo G. K., Codd G. A., 1988: Isolation and purification of peptide and alkaloid toxins from Anabaena flos-aquae using high-performance thin-layer chromatography. - Journal of Microbiological Methods, 7(6): 251-257.

An J., Carmichael W. W., 1994: Use of a colorimetric protein phosphatase inhibition assay and enzyme linked immunosorbent assay for the study of microcystins and nodularins. - Toxicon, 32(12): 1495-1507.

Bouaícha N., Maa touk I., Vincent G., Levy Y., 2002: A colorimetric and fluorimetric microplate assay for the detection of microcystin-LR in drinking water without preconcentration. - Food and Chemical Toxicology, 40(11): 1677-1683.

Dörr F. A., Pinto E., Soares R. M., Feliciano de Oliveirae Azevedo S. M., 2010: Microcystins in South American aquatic ecosystems: occurrence, toxicity and toxicological assays. - Toxicon, 56: 1247-1256.

Fastner J., Erhard M., van Døhren H., 2001: Determination of oligopeptide diversity within a natural population of Microcystis spp. (Cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization-time of flight mass spectrometry. - Applied and Environmental Microbiology, 67(11): 5069-5076.

Figueiredo D. R., Azeiteiro U. M., Esteves S. M., Gonçalves F. J. M., Pereira M. J., 2004: Microcystin-producing blooms - a serious global public health issue. - Ecotoxicology and Environmental Safety, 59: 151-163.

Hedman C. J., Krick W. R., Karner Perkins D. A., Harrahy E. A., Sonzogni W. C., 2008: New measurements of cyanobacterial toxins in natural waters using High Perfomance Liquid Chromatography coupled with Mass Spectrometry. - Journal of Environmental Quality, 37: 1817-1824.

Heinze R., Fastner J., Neumann U., Chorus I., 2001: Testing cyanobacterial toxicity with primary rat hepatocyte and cell-line assays. - In: Chorus I. (ed.), Cyanotoxins - Occurrence, Causes, Consequences: 317-324. - Springer Publishers Heidelberg.

Heresztyn T., Nicholson B. C., 2001: Determination of cyanobacterial hepatotoxins directly in water using a protein phosphatase inhibition assay. - Water Research, 35(13): 3049-3056.

Hisem D., Hrouzek P., Tomek P., Tomšicková J., Zapomelová E., Skácelová K., Lukešová A., Kopeck ý J., 2011: Cyanobacterial cytotoxicity versus toxicity to brine shrimp Artemia salina. - Toxicon, 57: 76-83.

Joung S. H., Oh H. M., Ko S. R., Ahn C. Y., 2011: Correlations between environmental factors and toxic and non-toxic Microcystis dynamics during bloom in Daechung Reservoir, Korea. - Harmful Algae, 10: 188-193.

Kaya K., Sano T., 1998: A photodetoxification mechanism of the cyanobacterial hepatotoxin microcystin-LR by ultraviolet irradiation. - Chemical Research in Toxicology, 11(3): 159-163.

Koskenniemi K., Lyra C., Raja niemi-Wack lin P., Jokela J., Sivonen K., 2007: Quantitative realtime PCR detection of toxic Nodularia cyanobacteria in the Baltic Sea. - Applied and Environmental Microbiology, 73: 2173-2179.

Kováts N., Ács A., Paulovits G., Vaa s G., 2011: Response of Lemna minor clones to Microcystis toxicity. - Applied ecology and environmental research, (1): 17-26.

Kurmayer R., Kutzenberger T., 2003: Application of real-time PCR for quantification of microcys tin genotypes in a population of the toxic cyanobacterium Microcystis sp. - Applied and Environmental Microbiology, 69: 6723-6730.

Kurmayer R., Christiansen G., 2009: The genetic basis of toxin production in cyanobacteria. - Freshwater Reviews, 2: 31-50.

Lambert T. W., Boland M. P., Holmes C. F. B., Hrudey S. E., 1994: Quantization of the microcystin hepatotoxins in water at environmentally relevant concentrations with the protein phosphatase bioassay. - Environmental Science & Technology, 28: 753-755.

Lawton L. A., Edwards C., 2008: Conventional laboratory methods for cyanotoxins. - Advances in Experimental Medicine and Biology, 619: 513-537.

Lawton L. A., Chambers H., Edwards C., Nwaopara A. A., Healy M., 2010: Rapid detection of microcystins in cells and water. - Toxicon, 55: 973-978.

Metcalf J. S., Bell S. G., Codd G. A., 2000: Production of novel polyclonal antibodies against the cyanobacterial toxin microcystin-LR and their application for the detection and quantification of microcystins and nodularin. - Water Research, 34(10): 2761-2769.

Metcalf J. S., Bell S. G., Codd G. A., 2001: Colorimetric immunoprotein phosphatase inhibition assay for specific detection of microcystins and nodularins of cyanobacteria. - Applied and Environmental Microbiology, 67(2): 904-909.

Metcalf J. S., Beattie K. A., Ressler J., Gerbersdorf S., Pflugmacher S., Codd G. A., 2002: Cross-reactivity and performance assessment of four microcystin immunoassays with detoxication products of the cyanobacterial toxin, microcystin-LR. - Journal of Water Supply Research and Technology-aqua, 51(3): 145-151.

Msagati T. A. M., Siame B. A., Shushu D. D., 2006: Evaluation of methods for the isolation, detection and quantification of cyanobacterial hepatotoxins. - Aquatic Toxicology, 78: 382-397.

Nicholson B. C., Burch M. D., 2001: Evaluation of analytical methods for detection quantification of cyanotoxins in relation to Australian drinking water guidelines. - Australia.

Oehrle S. A., Southwell B., Westrick J., 2010: Detection of various freshwater cyanobacterial toxins using ultra-performance liquid chromatography tandem mass spectrometry. - Toxicon, 55: 965-972.

Ojanperä I., Pelander A., Vuori E., Himberg K., Waris M., Niinivaara K., 1995: Detection of cyanobacterial hepatotoxins by TLC. - Journal of Planar Chromatography, 8: 69-72.

Ouellette A. J., Wilhelm S. A., 2003: Toxic cyanobacteria: the evolving molecular toolbox. - Front. Ecol. Environ., 1: 359-366.

Pan H., Song L., Liu Y., Borner T., 2002: Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environmental samples. - Archives of Microbiology, 178(6): 421-427.

Pelander A., Ojanperä I., Sivonen K., Himberg K., Waris M., Niinivaara K., Vouri E., 1996: Screening for cyanobacterial toxins in bloom and strain samples by thin-layer chromatography. - Water Research, 30: 1464-1470.

Pelander A., Ojanperä I., Vouri E., 1998: Analysis of cyanobacterial hepatotoxins by overpressured layer chromatography. - Journal of Planar Chromatography, 11: 365-369.

Poon G. K., Priestley I. M., Hunt S. M., Faw ell J. K., Codd G. A., 1987: Purification procedure for peptide toxins from the cyanobacterium Microcystis aeruginosa involving high-performance thinlayer chromatography. - Journal of Chromatography, 387: 551-555.

Poon K. F., Lam M. H., Lam P. K., Wong B. S., 2001: Determination of microcystins in cyanobacterial blooms by solid-phase microextractionhigh-performance liquid chromatography. - Environmental Toxicology and Chemistry, 20(8): 1648-1655.

Rinta-Kanto J. M., Ouelette A. J. A., Boyer G. L., Twiss M. R., Bridgeman T. B., Wilhelm S . W., 2005: Quantification of toxic Microcystis spp. during the 2003 and 2004 blooms in Western Lake Erie using quantitative real-time PCR. - Environmental Science and Technology, 39: 4198-4205.

Rudi K., Skulberg O. M., Larsen F., Jakobsen K., 1998: Quantification of toxic cyanobacteria in water by use of competitive PCR followed by sequence-specific labelling of oligonucleotide probes. - Applied and Environmental Microbiology, 66: 176-179.

Sano T., Nohara K., Shiraishi F., Kaya K., 1992: A method for micro-determination of total microcystin content in water blooms of cyanobacteria (blue-green algae). - International Journal of Environmental Analytical Chemistry, 49: 163-170.

Scorzetti G., Brand L. E., Hitchcock G. L., Rein K. S ., Sinigalliano C. D., Fell J. W., 2009: Multiple simultaneous detection of Harmful Algal Blooms (HABs) through a high throughput bead array technology, with potential use in phytoplankton community analysis. - Harmful Algae, 8: 196-211.

Sivonen K., 2008: Emerging high throughput analyses of cyanobacterial toxins and toxic cyanobacteria. Cyanobacterial Harmful Algal Blooms: state of the science and research needs. Chapter 24. - Advances in Experimental Medicine & Biology, 619: 547-566.

Spoof L., Karlsson K., Meriluoto J., 2001: Highperformance liquid chromatographic separation of microcystins and nodularin, cyanobacterial peptide toxins on C18 and amide C16 sorbents. - Journal of Chromatography A, 909(2): 225-236.

Spoof L., Neffling M. R., Meriluoto J., 2010: Fast separation of microcystins and nodularins on narrow-bore reversed-phase columns coupled to a conventional HPLC system. - Toxicon, 55: 954-964.

Tillett D., Parker D. L., Neilan B. A., 2001: Detection of toxigenicity by a probe for the microcystin synthetase a gene (mcyA) of the cyanobacterial genus microcystis: comparison of toxicities with 16S rRNA and phycocyanin operon (Phycocyanin Intergenic Spacer) phylogenies. - Appl. Environ. Microbiol., 67(6): 2810-2818.

Triantis T., Tsimeli K., Kaloudis T., Thanassoulias N ., Lytras E., Hiskia A., 2010: Development of an integrated laboratory system for the monitoring of cyanotoxins in surface and drinking waters. - Toxicon, 55: 979-989.

Tsutsumi T., Nagata S., Hasegawa A., Ueno Y., 2000: Immunoaffinity column as a clean-up tool for determination of trace amounts of microcystins in tap water. - Food and Chemical Toxicology, 38(7): 593-597.

Vaitomaa J., Rantala A., Halinen K., Rouhiainen L ., Tallberg P., Mokelke L., Sivonen K. 2003: Quantitative real-time PCR for determination of microcystin synthetase E copy numbers for Microcystis and Anabaena in Lakes. - Applied and Environmental Microbiology, 69: 7289-7297.

Vasas G., Gáspár A., Páger C., Surányi G., Máthé C., Hamvas M. M., Borbely G., 2004: Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis. - Electrophoresis, 25(1): 108-115.

Welker M., Fastner J., Erhard M., van Døhren H., 2002: Applications of MALDI-TOF MS analysis in cyanotoxin research. - Environmental Toxicology, 17(4): 367-374.

Wirsing B., Flury T., Wiedner C., Neumann U., Weck esser J., 1999: Estimation of the microcystin content in cyanobacterial field samples from German lakes using the colorimetric proteinphosphatase inhibition assay and RP-HPLC. - Environmental Toxicology, 14(1): 23-29.

Xu L. H., Lam P. K. S., Chen J. P., Xu J. M., Wong B. S . F., Zhang Y. Y., Wu R. S. S., Harada K. I ., 2000: Use of protein phosphatase inhibition assay to detect microcystins in Donghu Lake and a fish pond in China. - Chemosphere, 41: 53-58.

Yen H. K., Lin T. F., Liao P. C., 2011: Simultaneous detection of nine cyanotoxins in drinking water using dual solid-phase extraction and liquid chromatography-mass spectrometry. - Toxicon, 58(2): 209-218.

Yu F. Y., Liu B. H., Chou H. N., Chu F. S., 2002: Development of a sensitive ELISA for the determination of microcystins in algae. - Journal of Agricultural and Food Chemistry, 50(15): 4176-4182.

Zeck A., Weller M. G., Bursill D., Niessner R., 2001: Generic microcystin immunoassay based on monoclonal antibodies against Adda. - Analyst, 126(11): 2002-2007.

Zegura B., Sedmak B., Filipic M., 2003: Microcystin-LR induces oxidative DNA damage in human hepatoma cell line HepG2. - Toxicon, 41(1): 41-48.

Botanica

The Journal of Nature Research Centre

Journal Information

CiteScore 2017: 0.50

SCImago Journal Rank (SJR) 2017: 0.186
Source Normalized Impact per Paper (SNIP) 2017: 0.130

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 77 77 12
PDF Downloads 14 14 2