Physiological responses of peanut (Arachis hypogaea L.) cultivars to water deficit stress: status of oxidative stress and antioxidant enzyme activities

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From a field experiment, the changes in oxidative stress and antioxidant enzyme activities were studied in six Spanish peanut cultivars subjected to 25−30 days of water deficit stress at two different stages: pegging and pod development stages. Imposition of water deficit stress significantly reduced relative water content, membrane stability and total carotenoid content in all the cultivars, whereas total chlorophyll content increased at pegging stage but decreased at pod developmental stage. Chlorophyll a/b ratio increased under water deficit stress in most of the cultivars suggesting a greater damage to chlorophyll b rather than an increase in chlorophyll a content. Oxidative stress measured in terms of H2O2, superoxide radical content and lipid peroxidation increased under water deficit stress, especially in susceptible cultivars such as DRG 1, AK 159 and ICGV 86031. Relationship among different physiological parameters showed that the level of oxidative stress, in terms of production of reactive oxygen species, was negatively correlated with activities of different antioxidant enzymes such as superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and glutathione reductase. In conclusion, the study shows that water deficit stress at pod development stage proved to be more detrimental than at pegging stage. The higher activities of antioxidant enzymes in the tolerant cultivars like ICGS 44 and TAG 24 were responsible for protection of oxidative damage and thus provide better tolerance to water deficit stress.


  • AEBI, H., 1984: Catalase in vitro. Methods in Enzymology 105, 121-126.

  • AGARWAL, S., SAIRAM, R. K., SRIVASTAVA, G. C., MEENA, R. C., 2005: Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biologia Plantarum 49, 541-550.

  • ARJENAKI, F. G., JABBARI, R., MORSHEDI, A., 2012: Evaluation of drought stress on relative water content, chlorophyll content and mineral elements of wheat (Triticum aestivum L.) varieties. International Journal of Agriculture and Crop Sciences 4, 726-729.

  • ARNON, D. I., 1949: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology 24, 1-15.

  • ARUNYANARK, A., JOGLOY, S., AKKASAENG, C., VORASOOT, N., KESMALA, T., RAO, R. C. N., WRIGHT, G. C., PATANOTHAI, A., 2008: Chlorophyll stability is an indicator of drought tolerance in peanut. Journal of Agronomy and Crop Science 194, 113-125.

  • ASHRAF, M., 2010: Inducing drought tolerance in plants. Biotechnological Advances 28, 169-183.

  • BAJJI, M., LUTTS, S., KINET, J. M., 2001: Water defi cit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Science 160, 669-681.

  • BLACK, C. A., 1965: Methods of soil analysis: Part I physical and mineralogical properties. American Society of Agronomy, Madison, Wisconsin, USA.

  • BOOTE, K. J., 1982: Growth stages of peanut (Arachis hypogaea L.). Peanut Science 9, 35−40.

  • BOOTE, K. J., KETRING, D. L., 1990: Peanut. In: STEWART B. A., NIELSON D. R. (eds.), Irrigation of agricultural crops, 114-137. ASA-CSSA-SSSA, Madison.

  • BRADFORD, M., 1976: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72, 248-254.

  • CASTILLO, F. I., PENEL, I., GREPPIN, H., 1984: Peroxidase release induced by ozone in Sedum album leaves. Plant Physiology 74, 846-851.

  • CELIKKOL-AKCAY, U., ERCAN, O., KAVAS, M., YILDIZ, L., YILMAZ, C., OKTEM, H. A., YUCEL, M., 2010: Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings. Plant Growth Regulation 61, 21-28.

  • CHAITANYA, K. S. K., NAITHANI, S. C., 1994: Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn. New Phytologist 126, 623-627.

  • CHALLINOR, A. J., SLINGO, J. M., WHEELER, T. R., et al., 2003: Toward a combined seasonal weather and crop productivity forecasting system: determination of the working spatial scale. Journal of Applied Meteorology 42, 175-192.

  • CHAVES, M. M., FLEXAS, J., PINHEIRO, C., 2009: Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany 103, 551-560.

  • DHINDSA, R. S., PLUMB-DHINDSA, P., THORPE, T. A., 1981: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany 32, 93-101.

  • DHRUVE, J. J., VAKHARIA, D. N., SHUKLA, Y. M., 2009: Role of benzyladenine on oxidative enzyme system in groundnut. Indian Journal of Agricultural Biochemistry 22, 98-101.

  • ERICE, G., LOUAHLIA, S., IRIGOYEN, J. J., SANCHEZ-DIAZ, M., AVICE, J. C., 2010: Biomass partitioning, morphology and water status of four alfalfa genotypes submitted to progressive drought and subsequent recovery. Journal of Plant Physiology 167, 114-120.

  • FINI, A., GUIDI, L., FERRINI, F., BRUNETTI, C., FERDINANDOA, M. D., BIRICOLTI, S., POLLASTRI, S., CALAMAI, L., TATTINI, M., 2012: Drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in Fraxinus ornus leaves: An excess light stress affair? Journal of Plant Physiology 169, 929-939.

  • GOMEZ, K. A., GOMEZ, A. A., 1984: Statistical procedures for agriculture research. Willey, New York.

  • HEATH, R. L., PACKER, L., 1968: Photoperoxidation in isolated chloroplast. I. Kinetics and stoi chiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189-198.

  • HERNANDEZ, J. A., FERRER, M. A., JIMENEZ, A., BARCELO, A. R., SEVILLA, F., 2001: Antioxidant systems and O2-/H2O2 production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins. Plant Physiology 127, 817-831. HISCOX, J. D., ISRAELSTAM, G. F., 1979: A method for extraction of chloroplast from leaf tissue without maceration. Canadian Journal of Botany 57, 1332-1334.

  • HOREMANS, N., FOYER, C. H., POTTERS, G., ASARD, H., 2000: Ascorbate function and associated transport systems in plants. Plant Physiology and Biochemistry 38, 531-540.

  • JUBANY-MARI, T., MUNNE-BOSCH, S., ALEGRE, L., 2010: Redox regulation of water stress responses in field-grown plants. Role of hydrogen peroxide and ascorbate. Plant Physiology and Biochemistry 48, 351-358.

  • KHOLOVA, J., SAIRAM, R. K., MEENA, R. C., SRIVASTAVA, G. C. 2009: Response of maize genotypes to salinity stress in relation to osmolytes and metal-ions contents, oxidative stress and antioxidant enzymes activity. Biologia Plantarum 53, 249-256.

  • KNOX, J. P., DODGE, A. D., 1985: Singlet oxygen and plants. Phytochemistry 24, 889-896.

  • LESSANI, H., MOJTAHEDI, M., 2002: Introduction to plant physiology (Translation). 6th Ed, 726. Tehran University Press, Iran.

  • LICHTENTHALER, H. K., WELLBURN, A. R., 1985: Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biochemical Society Transactions 11, 591−592.

  • MAFAKHERI, A., SIOSEMARDEH, A., BAHRAMNEJAD, B., STRUIK, P. C., SOHRABI, Y., 2010: Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian Journal of Crop Science 4, 580-585.

  • MENSAH, J. K., OBADONI, B. O., EROUTOR, P. G., ONOME-IRIEGUNA, F., 2006: Simulated flooding and drought effects on germination, growth and yield parameters of sesame (Sesamum indicum L.). African Journal of Biotechnology 5, 1249-1253.

  • MITTLER, R., 2002: Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7, 405-410.

  • NAGESWARA RAO, R. C., TALWAR, H. S., WRIGHT, G. C., 2001: Rapid assessment of specific leaf area and leaf nitrogen in peanut (Arachis hypogaea L.) using chlorophyll meter. Journal of Agronomy and Crop Science 189, 175-182.

  • NAKANO, Y., ASADA, K., 1981: Spinach chloroplasts scavenge hydrogen peroxide on illumination. Plant and Cell Physiology 21, 1295-1307.

  • NAUTIYAL, P. C., RAVINDRA, V., RATHNAKUMAR, A. L., AJAY, B. C., ZALA, P. V., 2012: Genetic variations in photosynthetic rate, pod yield and yield components in Spanish peanut cultivars during three cropping seasons. Field Crops Research 125, 83-91.

  • NOCTOR, G., FOYER, C., 1998: Ascorbate and glutathione: keeping active oxygen under control. Annual Reviews of Plant Physiology and Molecular Biology 49, 249-279.

  • NOCTOR, G., GOMEZ, L., VANACKER, H., FOYER, C. H., 2002: Interaction between biosynthesis, compartmentation and transport in the control of glutathione homeostasis and signalling. Journal of Experimental Botany 53, 1283-1308.

  • PATTANGUAL, W., MADORE, M., 1999: Water deficit effects on raffinose family oligosaccharide metabolism in Coleus. Plant Physiology 121, 998-993.

  • PRASAD, T. K., ANDERSON, M. D., STEWART, C. R., 1995: Localization and characterization of peroxidases in the mitochondria of chilling acclimated maize seedlings. Plant Physiology 108, 1597-1605.

  • RAO M. V., PALIYATH, G., ORMROD, D. P., MURR, D. P., WATKINS, C. B., 1997: Influence of salicylic acid on H2O2 production, oxidative stress and H2O2 metabolizing enzymes. Plant Physiology 115, 137-149. SAIRAM, R. K., SAXENA, D. C., 2005: Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. Journal of Agronomy and Crop Science 184, 55-61.

  • SAIRAM, R. K., SRIVASTAVA, G. C., SAXENA, D. C., 2000: Increased antioxidant activity under elevated temperature: a mechanism of heat stress tolerance in wheat genotypes. Biologia Plantarum 43, 245-251.

  • SAIRAM, R. K., DESHMUKH, P. S., SHUKLA, D. S., 1997: Tolerance of drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy and Crop Science 178, 171-177.

  • SANTOS, I., ALMEIDA, J. M., 2011: Responses of plants at molecular, biochemical and ultrastructural levels as influenced by UV-B radiation. Advances in Plant Physiology 12, 1-30.

  • SCHWANZ, P., POLLE, A., 2001: Differential stress responses of antioxidative systems to drought in pedunculate oak (Quercus robur) and maritime pine (Pinus pineaster) grown under high CO2 concentrations. Journal of Experimental Botany 52, 133-143.

  • SHAO, H. B., CHU L. Y., LU, Z. H., KANG, C. M., 2008: Main antioxidants and redox signalling in higher plant cells. International Journal of Biological Sciences 44, 12-18.

  • SHARADA, P., NAIK, G. R., 2011: Physiological and biochemical responses of groundnut genotypes to drought stress. World Journal of Science and Technology 1, 60-66.

  • SHARIFI, P., AMIRNIA, R., MAJIDI, E., HADI, H., ROUSTAII, M., NAKHODA, B., ALIPOOR, H. M., MORADI, F., 2012: Relationship between drought stress and some antioxidant enzymes with cell membrane and chlorophyll stability in wheat lines. African Journal of Microbiology Research 6, 617-623.

  • SHARMA, P., DUBEY, R. S., 2005: Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation 46, 209-221.

  • SINGH, A. L., NAKAR, R. N, GOSWAMI, N., KALARIYA, K. A., CHAKRABORTY, K., SINGH, M., 2013: Water deficit stress and its management in groundnuts. Advances in Plant Physiology, 14, 370−465.

  • SMITH, I. K., VIERHELLER, T. L. I., THORNE, C. A., 1988: Assay of glutathione reductase in crude tissue homogenates using 5, 5’-dithio bis (2-nitrobenzoic acid). Analytical Biochemistry 175, 408-413.

  • TAIZ, L., ZEIGER, E., 2006: Plant physiology, 4th ed. Sinauer Associates, Sunderland, Massachusetts.

  • WANG, F. Z., WANG, Q. B., KWON, S. Y., KWAK, S. S., SU, W. A., 2005: Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. Journal of Plant Physiology 162, 465-472.

  • WEATHERLEY, P. E., 1950: Studies in water relations of cotton plants. I. The field measurement of water deficit in leaves. New Phytologist 49, 81-87.

  • YOUNG, A. J., 1991: The photo-protective role of carotenoids in higher plants. Physiologia Plantarum 83, 702-708.

  • ZHU, J. K., 2002: Salt and drought stress signal transduction in plants. Annual Reviews of Plant Biology 53, 247-273.

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