Stimulated Growth of Green Algae Haematococcus Pluvialis Under Different Environmental Conditions

Open access


Haematococcus pluvialis is a genus of green algae that keeps the high interest of the scientists for decades due to its capacity to synthesize astaxanthin. Among many others, astaxanthin is one of the most demanded natural anti-oxidant and the product of the future. It has the widest usage as source of nutrient rich food and health promoting compounds. Astaxanthin has got various applications in the nutraceuticals as immune system enhancer and anti-cancer agent, in the cosmetics as anti-aging component, as additive to food, as well as in the aquaculture industries. As a key requirement for the research in this field is to find out the better conditions and media to stimulate the H.pluvialis higher growth rate to reach in the field of microalgae economically sustainable cultivation and processing. The effect of WC versus BG-11 media to enhance growth rate of Haematococcus pluvialis has been examined. This study employed a parallel testing of stimulating effect of extra vitamins versus phosphorus. Combination of mentioned parameters with gentle shacking have been determined as multiple effectors that contribute to green algae cultures to grow to high density. Importantly, this study demonstrates that extra phosphorus contribute to growth rate of H.pluvilais positively, meanwhile addition of extra vitamins in combination with extra phosphorus impact both positively and negatively to promote cells division. Shacking influences the culture growth as either stimulator or repressor.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Cifuentes AS Gonzalez MA Vargas S Hoeneisen M Gonzalez N (2003) Optimization of biomass total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada USA) under laboratory conditions. Biol Res 36:343-357.

  • Esra I Fazilet VS Meltem CD (2007) Effect of different culture media and light intensities on growth of Haematococcus pluvialis. Int J Nat Eng Sci 1(3):05-09.

  • Fábregas J Otero A Maseda A Domínguez A (2000) Two-stage cultures for the production of astaxanthin from Haematococcus pluvialis. J Biotechnol 89(1):65-71.

  • Hillebrand H Dürselen CD Kirschtel D Pollingher U Zohary T (1999) Biovolume calculation for pelagic and benthic microalgae. J Phycol 35:403-424.

  • Holtin K et al. (2009) Determination of astaxanthin and astaxanthin esters in the microalgae Haematococcus pluvialis by LC-(APCI)MS and characterization of predominant carotenoid isomers by NMR spectroscopy. Anal Bioanal Che 395(6):1613-1622.

  • Kobayashi M Kakizono T Nagai S (1991) Astaxanthin production by a green alga Haematococcus pluvialis accompanied with morphological changes in acetate media. J Ferment Bioeng 71:335-339.

  • Li L et al. (2013) Effect of vitamin-B12 and vitamin-H on the growth and astaxanthin content of Haematococcus pluvialis CH-1. Advan J of Food Scien and Technol 5(9):1139-1142.

  • Lorenz RT Cysewski GR (2000) Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Trends in Biotech 18(4):160-167.

  • Lund JWG Kipling C Le Cren ED (1958) The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting. Hydrobiol 11:143-170.

  • Martín JF Gudiña E Barredo JL (2008) Conversion of β-carotene into astaxanthin: Two separate enzymes or a bifunctional hydroxylase-ketolase protein? Microbial Cell Factories 7:3.

  • Radmer RJ (1996) Algal diversity and commercial algal products. Bioscience 46:263-270.

  • Sandmann G (1994) Carotenoid biosynthesis in microorganisms and plants. Eur. J. Biochem. 223:7-24.

  • Margalith PZ (1999) Production of ketocarotenoids by microalgae. Appl. Microbiol. Biotechnol 51(4):431-438.

  • Shah MMR Liang Y Cheng JJ Daroch M (2016) Astaxanthin-Producing Green Microalga Haematococcus pluvialis: From Single Cell to High Value Commercial Products. Front Plant Sci 7:531.

  • Sipaúba-Tavares LH Berchielli-Morais FA Scardoeli-Truzzi B (2015) Growth of Haematococcus pluvialis Flotow in alternative media. Braz J Biol 75(4):796-803.

  • Sipaúba-Tavares LH Millan RN Berchielli-Morais FA (2013) Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow. Braz J Biol 73(3):585-591.

  • Skjanes K Rebours C Lindblad P (2013) Potential for green microalgae to produce hydrogen pharmaceuticals and other high value products in a combined process. Crit Rev Biotechnol 33:172-215.

  • Torzillo G Goksan T Faraloni C Kopecky J Masojidek J (2003) Interplay between photochemical activities and pigment composition in an outdoor culture of Haematococcus pluvialis during the shift from the green to red stage. J Appl Phycol 15:127-136.

  • Tripathi U Sarada R Ravishankar G (2002) Effect of culture conditions on growth of green alga - Haematococcus pluvialis and astaxanthin production. Acta Physiol Plant 24(3):323-329.

  • Ytrestoyl T Bjerkeng B (2007) Intraperitoneal and dietary administration of astaxanthin in rainbow trout (Oncorhynchus mykiss) − plasma uptake and tissue distribution of geometrical E/Z isomers. Comp Biochem Physiol B: Biochem Mol Biol 147:250-259.

Journal information
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 401 252 16
PDF Downloads 152 102 9