3D tubular scaffolds made from Poly-(Ɛ-caprolactone) (PCL)/chitosan (CS) nanofibres are very promising candidate as vascular grafts in the field of tissue engineering. In this work, the fabrication of PCL/CS-blended nanofibrous tubes with small diameters by electrospinning from separate PCL and CS solutions is studied. The influence of different CS solutions (CS/polyethylene glycol (PEO)/glacial acetic acid (AcOH), CS/trifluoroacetic acid (TFA), CS/ AcOH) on fibre formation and producibility of nanofibrous tubes is investigated. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) is used to verify the presence of CS in the blended samples. Tensile testing and pore size measurements are done to underline the good prerequisites of the fabricated blended PCL/ CS nanofibrous tubes as potential scaffolds for vascular grafts. Tubes fabricated from the combination of PCL and CS dissolved in AcOH possesses properties, which are favourable for future cell culture studies.
If the inline PDF is not rendering correctly, you can download the PDF file here.
 Sales KM, Salacinski HJ, Alobaid N, Mikhail M, Balakrishnan V and Am Seifalian. Advancing vascular tissue engineering: the role of stem cell technology. TRENDS IN BIOTECHNOLOGY 2005; 23: 461-467.
Torikai K, Ichikawa H, Hirakawa K, et al. A self-renewing, tissue-engineered vascular graft for arterial reconstruction. JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY 2008; 136: 37-U56.
Hasan A, Memic A, Annabi N, et al. Electrospun scaffolds for tissue engineering of vascular grafts. ACTA BIOMATERIALIA 2014; 10: 11-25.
Li WJ, Laurencin CT, Caterson EJ, Tuan RS and Ko FK. Electrospun nanofibrous structure: A novel scaffold for tissue engineering. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH 2002; 60: 613-621.
Yoshimoto H, Shin YM, Terai H and Vacanti JP. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. BIOMATERIALS 2003; 24: 2077-2082.
Shin M, Yoshimoto H and Vacanti JP. In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun nanofibrous scaffold. TISSUE ENGINEERING 2004; 10: 33-41.
Pillai, C. K. S., Paul W and Sharma CP. Chitin and chitosan polymers: Chemistry, solubility and fiber formation. PROGRESS IN POLYMER SCIENCE 2009; 34: 641-678.
Geng XY, Kwon OH and Jang JH. Electrospinning of chitosan dissolved in concentrated acetic acid solution. BIOMATERIALS 2005; 26: 5427-5432.
Cheng T, Hund R, Aibibu D, Horakova J and Cherif C. Pure chitosan and chitosan/chitosan lactate blended nanofibres made by single step electrospinning. AUTEX RESEARCH JOURNAL 2013; 13: 128-133.
Ohkawa K, Di Cha, Kim H, Nishida A and Yamamoto H. Electrospinning of chitosan. MACROMOLECULAR RAPID COMMUNICATIONS 2004; 25: 1600-1605.
Spasova M, Manolova N, Paneva D and Rashkov I. Preparation of chitosan-containing nanofibres by electrospinning of chitosan/poly(ethylene oxide) blend solutions. E-POLYMERS 2004.
Shalumon KT, Anulekha KH, Girish CM, Prasanth R, Nair SV and Jayakumar R. Single step electrospinning of chitosan/poly(caprolactone) nanofibers using formic acid/ acetone solvent mixture. CARBOHYDRATE POLYMERS 2010; 80: 413-419.
Van der Schueren, Lien, Steyaert I, Schoenmaker B de and Clerck K de. Polycaprolactone/chitosan blend nanofibres electrospun from an acetic acid/formic acid solvent system. CARBOHYDRATE POLYMERS 2012; 88: 1221-1226.
Chen H, Huang J, Yu J, Liu S and Gu P. Electrospun chitosan-graft-poly (epsilon-caprolactone)/poly (epsiloncaprolactone) cationic nanofibrous mats as potential scaffolds for skin tissue engineering. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2011; 48: 13-19.
Cooper A, Jana S, Bhattarai N and Zhang M. Aligned chitosan-based nanofibers for enhanced myogenesis. JOURNAL OF MATERIALS CHEMISTRY 2010; 20: 8904- 8911.
Roozbahani F, Sultana N, Ismail AF and Nouparvar H. Effects of Chitosan Alkali Pretreatment on the Preparation of Electrospun PCL/Chitosan Blend Nanofibrous Scaffolds for Tissue Engineering Application. JOURNAL OF NANOMATERIALS 2013.
Jana S and Zhang M. Fabrication of 3D aligned nanofibrous tubes by direct electrospinning. JOURNAL OF MATERIALS CHEMISTRY B 2013; 1: 2575-2581.
Du F, Wang H, Zhao W, et al. Gradient nanofibrous chitosan/ poly epsilon-caprolactone scaffolds as extracellular microenvironments for vascular tissue engineering. BIOMATERIALS 2012; 33: 762-770.
Yao Y, Wang J, Cui Y, et al. Effect of sustained heparin release from PCL/chitosan hybrid small-diameter vascular grafts on anti-thrombogenic property and endothelialization. ACTA BIOMATERIALIA 2014; 10: 2739-2749.
Huang C, Chen R, Ke Q, Morsi Y, Zhang K and Mo X. Electrospun collagen-chitosan-TPU nanofibrous scaffolds for tissue engineered tubular grafts. COLLOIDS AND SURFACES B-BIOINTERFACES 2011; 82: 307-315.
Holzapfel GA, Sommer G, Gasser CT and Regitnig P. Determination of layer-specific mechanical properties of human coronary arteries with nonatherosclerotic intimal thickening and related constitutive modeling. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 2005; 289: H2048-H2058.