Enzyme-Assisted Extraction of Polyphenols From Rose (Rosa Damascena Mill.) Petals

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Abstract

: The efficiency of enzyme-assisted extraction for the recovery of polyphenols from rose (Rosa damascena Mill.) petals was evaluated performing a simplex centroid experimental design for mixture with three components (pectinolytic, cellulolytic and hemicellulolytic preparation). The ternary enzyme combinations leaded to the highest contents of total polyphenols, reaching 43% higher average value as compared to the control (without enzymatic treatment) sample. Enzymatic treatments also enhanced (9−25%) the extractability of total anthocyanins. The results obtained demonstrate that enzyme-assisted extraction improves the recovery of polyphenolic antioxidants from rose petals, especially using ternary enzyme combinations, comprising pectinolytic, cellulolytic and hemicellulolytic preparation.

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  • 1. Anesini C. & Perez C. (1993). Screening of plants used in Argentine folk medicine for antimicrobial activity. J. Ethnopharmacol. 39 119−128. DOI: 10.1016/0378-8741(93)90027-3.

  • 2. Benzie I.F.F. & Strain J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry 239(1) 70−76. DOI: 10.1006/abio.1996.0292.

  • 3. Brand-Williams W. Cuvelier M.E. & Berset C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28(1) 25−30. DOI: 10.1016/S0023-6438(95)80008-5.

  • 4. Cho E.J. Yokozawa T. Rhyu D.Y. Kim S.C. Shibahara N. & Park J.C. (2003). Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 11-diphenyl-2-picrylhydrazyl radical Phytomedicine 10(6-7) 544−551. DOI: 10.1078/094471103322331520.

  • 5. Choi E.M. & Hwang J.K. (2003). Investigations of anti-inflammatory and antinociceptiveactivities of Piper cubeba Physalis angulata and Rosa hybrida. J. Ethnopharmacol. 89(1) 171−175. DOI: 10.1016/S0378-8741(03)00280-0.

  • 6. Dinkova R. Shikov V. Mihalev K. Velchev Z. Dinkov H. & Mollov P. (2012). Changes in the total anthocyanins and polyphenols during processing of wild berries into freshly pressed juices. Journal of EcoAgriTourism 8(1) 254−259.

  • 7. Dixit N. Tripathi C. & Upadhyay R. (1976). The antifungal substance of rose flowers (Rosa indica). Econ. Bot. 30 371−374. DOI:10.1007/BF02904658.

  • 8. Dobrev G. & Zhekova B. (2012). Biosynthesis purification and characterization of endodlucanase from xylanase produsing strain Aspergilus niger B03. Brazilian Journal of Microbiology 43(1) 70−77. DOI: 10.1590/S1517-83822012000100008.

  • 9. Dobrev G. Pishtiyski I. Stanchev V. & Mircheva R. (2007) Optimization of nutrient medium containing agricultural wastes for xylanase production by Aspergillus niger B03 using optimal composite design. Bioresource Technology 98(14) 2671−2678. DOI:10.1016/j.biortech.2006.09.022.

  • 10. Fenglin H. Ruili L. Bao H. & Liang M. (2004). Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia 75(1) 14−23. DOI: 10.1016/j.fitote.2003.07.003.

  • 11. Ginova A. Mihalev K. & Kondakova V. (2013). Antioxidant capacity of petals and leaves from different rose (Rosa damascena Mill.) plantations in Bulgaria. International Journal of Pure Applied Bioscience 1 38−43.

  • 12. Giusti M.M. & Wrolstad R.E. (2001). Characterization and measurement of anthocyanins by UV-visible spectroscopy. In R.E. Wrolstad (Ed.) Current Protocols in Food Analytical Chemistry (pp. F1.2.1−F1.2.13) New York: Wiley.

  • 13. Kaverzneva E.D. (1971). Assay for determination of proteolytic activity of complex enzymes and proteinases. Prikladnaja biochimia i Mikrobiologia 7 225−228 (in Rusian).

  • 14. Magalhães L.M. Segundo M.A. Reis S. & Lima J.L.F.C. (2008). Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta 613(1) 1−19. DOI: 10.1016/j.aca.2008.02.047

  • 15. Mahmood N. Piacente S. Pizza C. Burke A. Khan A.I. & Hay A.J. (1996). The anti-HIVactivity and mechanisms of action of pure compounds isolated from Rosa damascena. Biochem. Biophys. Res. Comm. 229(1) 73−79. DOI: 10.1006/bbrc.1996.1759.

  • 16. Miller G.L. (1959). Use of dinitrosalicyclic acid reagent for determination of reducing sugar. J. Anal. Chem. 31(3) 426−428. DOI: 10.1021/ac60147a030.

  • 17. Miron T.L. Herrero M. & Ibáñez E. (2013). Enrichment of antioxidant compounds from lemon balm (Melissa officinalis) by pressurized liquid extraction and enzyme-assisted extraction. Journal of Chromatography A 1288(3) 1−9. DOI: 10.1016/j.chroma.2013.02.075.

  • 18. Ng T.B. He J.S. Niu S.M. Zhao L. Pi Z.F. Shao W.& Liu F. (2004). A gallic acid derivative and polysaccharides with antioxidative activity from rose (Rosa rugosa) flowers. J. Pharm. Pharmacol. 56 537−545. DOI: 10.1211/0022357022944.

  • 19. Perez C. & Anesini C. (1994). In vitro antibacterial activity of Argentine folk medicinal plants against Salmonella typhi. J. Ethnopharmacol. 44(1) 41−46. DOI: 10.1016/0378-8741(94)90097-3.

  • 20. Pinelo-Jiménez M. & Meyer A.S. (2008). Enzyme-assisted extraction of antioxidants: Release of phenols from vegetal matrixes. Electronic Journal of Environmental Agricultural and Food Chemistry 7(8) 3217−3220.

  • 21. Ponpium P. Ratanakhanokchai K. & Kyu K. (2000). Isolation and properties of a cellulosome-type multienzyme complex of the thermophilic Bacteroides sp. strain P-1. Enzyme Microbial. Technol. 26(5-6) 459−465. DOI: 10.1016/S0141-0229(99)00195-7.

  • 22. Singleton V.L. & Rossi J.A. Jr. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16 144−158.

  • 23. VanderJagt T.J. Ghattas R. VanderJagt D.J. Crossey M. & Glew R.H. (2002). Comparison of the total antioxidant content of 30 widely used medicinal plants of New Mexico. Life Sci. 70(9) 1035−1040 DOI: 10.1016/S0024-3205(01)01481-3.

  • 24. Vinokur Y. Rodov V. Reznick N. Goldman G. Horev B. Umiel N. & Friedman H. (2006). Rose petal tea as an antioxidant-rich beverage: cultivar effects J. Food Sci. 71 S42−S47. DOI: 10.1111/j.1365-2621.2006.tb12404.x.

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