Green-extraction of essential oil of the species Ruta chalepensis L.: gas chromatography-mass spectroscopy-infra red analysis and response surface methodology optimization

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The objective of the present study was the optimization of the parameters affecting the hydrodistillation of Ruta chalepensis L. essential oil using response surface design type Box-Behnken. After an appropriate choice of three parameters, 15 experiments were performed leading to a mathematical second-degree model relating the response function (yield of essential oil) to parameters and allowing a good control of the extraction process. The realization of the experiments and data analysis was carried out by response surface methodology (RSM). A deduced second-order polynomial expression was used to determine the optimal conditions necessary to obtain a better essential oil yield. These optimized operating conditions were: a granulometry of 2 mm, a condensation-water flow rate of 3.4 mL/min and an extraction time of 204 min. Analysis of variance (ANOVA) indicates that the generated second-order polynomial model was highly significant with R2=0.9589 and P<0.006. The gas chromatography-mass spectrometry analysis of essential oil extracted from the Ruta chalepensis L. aerial parts revealed the presence of 2-undecanone, 2-nonanone and 2-decanone as major components.

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  • [1] Bourrain J.L. « Allergies aux huiles essentielles : aspects pratiques » Revue française d’allergologie201353 30-32.

  • [2] Babar A.; Naser A.A.; Saiba S.; Aftab A.; Shah A.K.; Firoz A. “Essential oils used in aromatherapy: A systemic review” Asian Pac J Trop Biomed. 20155(8) 601–61.

  • [3] Jacqueline S. « Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA) » 2009 Université de La Réunion. Colloque GP3A-Tananarive.

  • [4] Benbelaid F.; Khadir A.; Abdoune M.A.; Bendahou M.; Muselli A.; Costa J. “Antimicrobial activity of some essential oils against oral multidrug resistant Enterococcus faecalis in both planktonic and biofilm state” Asian Pacific Journal of Tropical Biomedicine20144(6) 463-472.

  • [5] Nashwa T.; Hassan H.M.; Sameh M.M.A.; Radwan I.A.; Hammouda O.; El-Gendy A.O. “Comparative chemical and antimicrobial study of nine essential oils obtained from medicinal plants growing in Egypt” Journal of basic and applied sciences20143 149-156.

  • [6] Glisic S.; Jasna I.; Mihajlo R.; Dejan S. “Extraction of sage (Salvia officinalis L.) by supercritical CO2: Kinetic data chemical composition and selectivity of diterpenes” J. of Supercritical Fluids201052 62–70.

  • [7] Pavlić B.; Vidović S.; Vladić J.; Radosavljević R.; Cindrić M.; Zeković Z. “Subcritical water extraction of sage (Salvia officinalis L.) by products Process optimization by response surface methodology” Journal of Supercritical Fluids2016116 36–45.

  • [8] Mu’azu K.; Mohammed-Dabo I.A.; Waziri S.M. “Development of Mathematical Model for the Prediction of Essential Oil Extraction from Eucalyptus Citriodora Leave” Journal of Basic and Applied Scientific Research20122(3) 2298-2306.

  • [9] Aleksic V.; Knezevic P. “Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L.” Microbiological Research2014169 240–254.

  • [10] Galadima M.S.; Ahmed A.S.; Olawale A.S.; Bugaje I.M. “Optimization of Steam Distillation of Essential Oil of Eucalyptus tereticornis by Response Surface Methodology” Nigerian Journal of Basic and Applied Science201220(4) 368-372.

  • [11] Mastura A.M.; Najwa M.; Khatimah M. “Supercritical Fluid Extraction of Citronella Oil from Cymbopogon nardus and its Optimization” Business Engineering and Industrial Applications Colloquium20139 73-78.

  • [12] Akalın M.K.; Tekin K.; Akyüz M.; Karagöz S. “Sage oil extraction and optimization by response surface methodology” Industrial Crops and Products201576 829–835.

  • [13] Timung R.; Barik C.R.; Purohit S.; Vaibhav V.G. “Composition and anti-bacteial activity analysis of citronella oil obtained by hydrodistillation: Process optimization study” Industrial Crops and Products201694 178–188.

  • [14] Afsaneh M.; Mina A. “Application of response surface methodology: design of experiments and optimization: a mini review” Indian Journal of Fundamental and Applied Life Sciences20144 2434-2439.

  • [15] Daniel G.; Verônica M. “The use and importance of design of experiments (DOE) in process modelling in food science and technology” Mathematical and Statistical Methods in Food Science and Technology First Edition. 2014.

  • [16] Vijaya C.S.V.; Maravajhala V. “Response surface methodology during optimization studies - an overview” Journal of Scientific Research in Pharmacy 20165(9) 124-129.

  • [17] Quezel P.; Santa S. « Nouvelle flore de l’Algérie et des régions désertiques méridionales » Tome II-Ed. CNRS Paris. 1963.

  • [18] Merghache S. Hamza M. & Tabti B. Etude physicochimique de l’huile essentielle de Ruta chalepensis L. de Tlemcen Algérie. Afrique science. 2009 5(1): 67-81.

  • [19] Liu S.; Yang F.; Zhang C.; Ji H.; Hong H.; Deng C. “Optimization of process parameters for supercritical carbon dioxide extraction of Passiflora seed oil by response surface methodology” Journal of Supercritical Fluids200948 9–14.

  • [20] Fadil M.; Farah A.; Ihssane B.; Haloui T.; Rachiq S. “Optimization of parameters influencing the hydrodistillation of Rosmarinus officinalis L. by response surface methodology” J. Mater. Environ. Sci. 20156(8) 2346-2357.

  • [21] Hancco V.; Poilâne C.; Chen J. « In 17èmes Journées Nationales sur les Composites Poitiers Futuroscope » France. 2011.

  • [22] Chopra S.; Gayathri V.P.; Sanjay K.M. “Release modulating hydrophilic matrix systems of losartan potassium: Optimization of formulation using statistical experimental design” European Journal of Pharmaceutics and Biopharmaceutics200766 73–82.

  • [23] Wijngaard H.H.; Brunton N. “The optimisation of solid–liquid extraction of antioxidants from apple pomace by response surface methodology” Journal of Food Engineering201096 134–140.

  • [24] Bendriss H. « Valorisation des extrais de plantes aromatiques et médicinales de : “Ruta chalepensis et Marrubium Vulgare” Mémoire de magister. Université Hassiba Ben Bouali Chlef (Algérie). 2003.

  • [25] Attou A. « Contribution à l’étude phytochimique et activités biologiques des extraits de la plante Ruta chalepensis (Fidjel) de la région d’Ain Témouchent » Mémoire de magister. Université Abou Bekr Belkaid Tlemcen (Algérie). 2011.

  • [26] Majdoub O.; Dhen N.; Souguir S.; Haouas D.; Baouandi M.; Laarif A.; Chaieb I. « Chemical composition of Ruta chalepensis essential oils and their insecticidal activity against Tribolium castaneum » Tunisian Journal of Plant Protection20149(1) 83-90.

  • [27] Baser K.H.C.; Özek T.; Beis S.H. “Constituents of the Essential Oil of Ruta chalepensis L. from Turkey” J. Essent. Oil Res.19968 413-414.

  • [28] Inigo R.P.A.; Be Viana M. E. L.; Catalan C.A.N.; De Iglesias D.I.A. “Essential oil of Ruta chalepensis L.(Rutaceae)” Essenze Deriv. Agrum. 198151 349-351.

  • [29] Rustaiyan A.; Khossravi M.; Sultani-Lotfabadi F.; Yari M.; Masoudi S.; Monfared A. “Constituents of the essential oil of Ruta chalepensis L. from Iran” J. Essent. Oil Res. 200214 378-379.

  • [30] Bagchi G.D.; Dwivedi P.D.; Mandal S.; Naqvi A.A. Kumar S. “Essential oil constituents of Ruta chalepensis L. plants grown in India” Indian Perfumer200347 39-41.

  • [31] Verzera V.; Mondello L.; Ragusa S.; Dugo G. “Essential oil from leaves oftypical Mediterranean plants. Note II Ruta chalepensis L” Essenze derivati agrumari200070 207–210.

  • [32] Baghlaf A.O.; El-Beihand S.K.A.; El-Tawil B.A.H. “Constituents of local plants. Part 75. study of volatile oil of Saudi Ruta chalepensis L. Juniperus procera Hochst. ex Endle. and Euphorbia heliofscopia L” Herba Hung. 198322 39-42.

  • [33] Pintore G.; Usai M.; Bradesi P.; Juliano C.; Boatto G.; Tomi F.; Chessa M.; Cerri R.; Casanova J. “Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica” Flavour and Fragrance Journal200217 15-19.

  • [34] Bourkhiss M.; Hnach M.; Bourkhiss B.; Ouhssine M.; Chaouch A.; Satrani B. « Effet de séchage sur la teneur et la composition chimique des huiles essentielles de Tetraclinis articulata (Vahl) Masters » Grosolutions200920(1) 44-48.

  • [35] Suzana F.A.; Leonardo L.B.; Joelma A.M.; Roberto F.V.; Pedro H.F.; Renê O.C.; José R.P.; Maria T.F. “Chemical variability of the essential oils from fruits of Pterodon emarginatus in the Brazilian Cerrado” Brazilian Journal of Pharmacognosy 201323(2) 224-229.

  • [36] Bampouli A.; Kyriakopoulou K.; Papaefstathiou G.; Louli V.; Krokida M.; Magoulas K. “Comparison of different extraction methods of Pistacia lentiscus var. chia leaves: Yield antioxidant activity and essential oil chemical composition” Journal of Applied Research on Medicinal and Aromatic Plants20141 81–91.

  • [37] Atailia I.; Djahoudi A. « Composition chimique et activité antibactérienne de l’huile essentielle de géranium rosat (Pelargonium graveolens L’Hér.) cultivé en Algérie » Phytothérapie201513 156–162

  • [38] Santos D.L.; Heleno D.F.; Leonardo L.B.; José R.P.; Leonice M.F.; Pierre A.S.; Pedro H.F.; Stone S.; Tatiana S.F. “Chemical composition of essential oils of leaves flowers and fruits of Hortia oreadica” Revista Brasileira de Farmacognosia 201626 23–28.

  • [39] Guan X.; Yao H. “Optimization of Viscozyme L-assisted extraction of oat bran protein using response surface methodology” Food Chemistry2008106 345–351.

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