Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

Open access

Abstract

The effects of the carbon and nitrogen substrates on the growth of Bacillus sp. SG113 strain were studied. The use of organic nitrogen sources (peptone, beef extract, yeast extract, casein) leads to rapid cellular growth and the best results for the Bacillus strain were obtained with casein hydrolysate. From the inorganic nitrogen sources studied, the (NH4) 2SO4 proved to be the best nitrogen source. Casein hydrolysate and (NH4) 2SO4 stimulated the invertase synthesis. In the presence of Jerusalem artichoke, onion and garlic extracts as carbon sources the strain synthesized from 6 to 10 times more inulinase.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1]. Gupta A.K.; Kaur N. Fructan storing plants - a potential source of high fructose syrups. J. Sci. Ind. Res. 1997 56(8) 447-452.

  • [2]. Hendry G. Evolutionary origins and natural functions of fructans: a climatological biogeographic and mechanistic appraisal. New Phytol. 1993 123(1) 3-14.

  • [3]. Vandamme E.J.; Derycke D.G. Microbial inulinases: Fermentation process properties and applications. Adv. Appl. Microbiol. 1983 29 139-176.

  • [4]. Bajpai P.; Margartis A. Production of high fructose syrups from Jerusalem artichoke tubers using Kluyveromyces marxianus immobilized in agar gel. J. Gen. Appl. Microbiol. 1985 31 305-311.

  • [5]. Pandey A.; Soccol C.R.; Selvakumar P.; Soccol V.T.; Krieger N.; Fontana J.D. Recent developments in microbial inulinases its production properties and industrial applications. Appl. Biochem. Biotechnol. 1999 81(1) 35-52.

  • [6]. Neagu-Bonciu C.; Bahrim G. Inulinases - a versatile tool for biotechnology. Innov. Romanian Food Biotechnol. 2011 9 1-11.

  • [7]. Kaur N.; Kaur M.; Gupta A.K.; Singh R. Properties of β-fructosidases (invertases and inulinases) of Fusarium oxysporum grown on an aqueous extract of Cichorium intybus roots. J. Chem. Technol. Biotechnol. 1992 53 279-284.

  • [8]. Wei B.; Hara M.; Yamauchi R.; Ueno Y.; Kato K. Fructooligosaccharids in the tubers of Jerusalem artichoke and Yacon. Res. Bull. Faculty Agri. Gifu Uni. 1991 56 133-138.

  • [9]. Chi Z.; Zhang T.; Liu G.; Xue L. Inulinase-expressing microorganisms and applications of inulinases. Appl. Microbiol. Biotechnol. 2009 82(2) 211-220.

  • [10]. Kango N.; Jain S.C. Production and properties of microbial inulinases: recent advances. Food Biotechnol. 2011 25(3) 165-212.

  • [11]. Jain S.C.; Jain P.C.; Kango N. Production of inulinase from Kluyveromyces marxianus using Dahlia tuber extract. Brazilian J. Microbiol. 2011 43(1) 62-69.

  • [12]. Zittan L. Enzymatic hydrolysis of Inulin-An alternative way to fructose production. Starch-Stärke 1981 33(11) 373-377.

  • [13]. Sangeethaa P.T.; Ramesha M.N.; Prapulla S.G. Recent trends in the microbial production analysis and application of Fructooligosaccharides. Trends Food Sci. Technol. 2005 16 442-457.

  • [14]. Skowronek M.; Fiedurek J. Inulinase biosynthesis using immobilized mycelium of Asperg illus niger. Enzyme Microb. Technol. 2006 38 162-167.

  • [15]. Uzunova K.; Vassileva A.; Kambourova M.; Ivanova V.; Spasova D.; Mandeva R.; Derekova A.; Tonkova A. Production and properties of a bacterial thermostable exo-inulinase. Zeitschrift für Naturforschung C 2001 56(11-12) 1022-1028.

  • [16]. Zherebtsov N.A.; Shelamova S.A.; Abramova I.N. Biosynthesis of inulinases by Bacillus bacteria. Prikladnaya Biokhimiya i Mikrobiologiya 2002 38(6) 634-638 (in Russian).

  • [17]. Sharma A.D.; Kainth S.; Gill P.K. Inulinase production using garlic (Allium sativum) powder as a potential substrate in Streptomyces sp. J. Food Eng. 2006 77(3) 486-491.

  • [18]. Singh R.S.; Sooch B.S.; Puri M. Optimization of medium and process parameters for the production of inulinase from a newly Isolated Kluyveromyces marxianus YS-1. Biores. Technol. 2007 98 2518-2525.

  • [19]. Yuan W.; Bai F. Optimization of medium and process parameters for the production of inulinase from Kluyveromyces marxianus Y1. J. Biotechnol. 2008 136S S317-S318.

  • [20]. Singh P.; Gill P.K. Production of inulinases: recent advances. Food Technol. Biotechnol. 2006 44 (2) 151-162.

  • [21]. Gavrailov S.; Ivanova V. Isolation and characteristics of a thermophilic Bacillus strain producer of inulinase. J. BioSci. Biotechnol. 2014 SE/ONLINE 83-94.

  • [22]. Miller G.L Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 1959 3 1426-1428.

Search
Journal information
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 277 83 3
PDF Downloads 125 56 3