Soil enzyme activities are “sensors” of soil organic matter (SOM) decomposition since they integrate information about microbial status and physico-chemical condition of soils. We measured dehydrogenase enzyme activity in a deciduous temperate oak forest in Hungary under litter manipulation treatments. The Síkfőkút Detritus Input and Removal Treatments (DIRT) Project includes treatments with doubling of leaf litter and woody debris inputs as well as removal of leaf litter and trenching to prevent root inputs. We hypothesized that increased detrital inputs increase labile carbon substrates to soils and would increase enzyme activities particularly that of dehydrogenase, which has been used as an indicator of soil microbial activity. We also hypothesized that enzyme activities would decrease with detritus removal plots and decrease labile carbon inputs to soil. After ten years of treatments, litter removal had a stronger effect on soil dehydrogenase activity than did litter additions. These results showed that in this forest ecosystem the changed litter production affected soil microbial activity: reduced litter production decreased the soil dehydrogenase activity; increased litter production had no significant effect on the enzyme activity.
ANTAL, E. - BERKI, I. - JUSTYÁK, J. - KISS, GY. - TARR, K. - VIG, P. (1997): A síkfőkúti erdőtársulás hő- és vízháztartási viszonyainak vizsgálata az erdőpusztulás és az éghajlatváltozás tükrében, Debrecen. pp 83. (in Hungarian).
AON, M. A. - COLANER, A. C. (2001): Temporal and spatial evolution of enzymatic activities and physico-chemical properties in an agricultural soil. Applied Soil Ecology 18: 255-270.
BARUAH, M. - MISHRA, R. R. (1984): Dehydrogenase and urease activities in rice field soils. Soil Biology and Biochemistry 16: 423-424.
BAUM, C. - LEINWEBER, P. - SCHLICHTING, A. (2003): Effects of chemical conditions in re-wetted peats temporal variation in microbial biomass and acid phosphatase activity within the growing season. Applied Soil Ecology 22: 167-174.
BŁOŃSKA, E. - LASOTA, J. - JANUSZEK, K. (2013): Variability of enzymatic activity in forest Cambisols and Brunic Arenosols of Polish lowland areas. Soil Science Annual 64: 54-59.
BONANOMIA, G. - D’ASCOLIC, R. - ANTIGNANIA, V. - CAPODILUPOA, M. - COZZOLINOA, L. - MARZAIOLIC, R. - PUOPOLOA, G. - RUTIGLIANOC, F. A. - SCELZAB, R. - SCOTTI, R. - RAOB, M.
A. - ZOINAA, A. (2011): Assessing soil quality under intensive cultivation and tree orchards in Southern Italy. Applied Soil Ecology 47: 184-194.
BRANT, J. B. - MYROLD, D. D. - SULZMAN, E. W. (2006b): Root controls on soil microbial community structure in forest soils. Oecologia 148: 650-659.
BRANT, J. B. - SULZMAN, E. W. - MYROLD, D. D. (2006a): Microbial community utilization of added carbon substrates in response to long-term carbon input manipulation. Soil Biology and Biochemistry 38: 2219-2232.
CHENDRAYAN, K. - AHLYA, T. K. - SETHUNATHAN, N. (1979): Dehydrogenase and invertase activities of flooded soils. Soil Biology and Biochemistry 12: 217-273.
CHODAK, M. - NIKLINSKA, M. (2010): Effect of texture and tree species on microbial properties of mine soils. Applied Soil Ecology 46: 268-275.
COLPAERT, J. V. - VAN LAERE, A. (2006): A comparison of the extracellular enzyme activities of two ectomycorrhizal and a leaf-saprotrophic basidiomycete colonizing beech leaf litter. New Phytologist 134: 133-141.
COURTY, P. E. - POUYSEGUR, R. - BUÉE, M. - GARBAYE, J. (2006): Laccase and phosphatase activities of the dominant ectomycorrhizal types in a lowland oak forest. Soil Biology and Biochemistry 38: 1219-1222.
DKHAR, M. S. - MISHRA, R. R. (1983): Dehydrogenase and urease activities of maize (Zea mays L.) filed crops. Plant and Soil 70: 327-333.
FEKETE, I. - VARGA, CS. - KOTROCZÓ, ZS. - KRAKOMPERGER, ZS. - TÓTH, J. A. (2007): The effect of temperature and moisture on enzyme activity in Síkfőkút Site. Cereal Research Communication 35: 381-385.
FEKETE, I. - VARGA, CS. - KOTROCZÓ, ZS. - TÓTH, J. A. - VÁRBIRÓ, G. (2011): The relation between various detritus inputs and soil enzyme activities in a Central European deciduous forest.
Geoderma 167-168: 15-21.
GÖRRES, J. H. - DICHIARO, M. J. - LYONS, J. B. - AMADOR, J. A. (1998): Spatial and temporal patterns of soil biological activity in a forest and an old field. Soil Biology and Biochemistry 30: 219-230.
JAKUCS, P (ed.) (1985): Ecology of an Oak Forest in Hungary I. Akadémiai Kiadó, Budapest.
KANDELER, E. (1996): Nitrate. In: Schinner, F. - Öhlinger, R. - Kandeler, E. - Margesin, R. (ed).
Methods in soil biology. Springer. Berlin, Heidelberg, New York. pp. 408-410.
KOTROCZÓ, ZS. - FEKETE, I. - TÓTH, J. A. - TÓTHMÉRÉSZ, B. (2008): Effect of leaf- and root-litter manipulation for carbon-dioxide efflux in forest soil. Cereal Research Communication 36: 663-666.
KOTROCZÓ, ZS. - VERES, ZS. - FEKETE, I. - PAPP, M. - TÓTH, J. A. (2012): Effects of Climate Change on Litter Production in a Quercetum petraeae-cerris Forest in Hungary. Acta Silvatica et Ligniaria Hungarica 8: 31-38.
KOVÁCS-LÁNG, E. - HERODEK, S. - TÓTH, J. A. (2000): Long Term Ecological Research in Hungary.
In: The International Long Term Ecological Research Network. Perspectives from Participating Networks. Compiled by the US LTER Network Office Albuquerque New Mexico. 38-40.
KUMAR, S. - CHAUDHURI, S. - MAITI, S. K. (2013): Soil Dehydrogenase Enzyme Activity in Natural and Mine Soil - A Review. Middle-East Journal of Scientific Research 13: 898-906.
LADD, J. N. (1985): Soil enzymes. In: Soil Organic Matter and Biological Activity. Nijhoff, Dordecht MAKOI, J. H. J. R. - NDAKIDEMI, P. A. (2008): Selected soil enzymes: Examples of their potential roles in the ecosystem. African Journal of Biotechnology 3: 181-191.
MAMATHA, G. - JAYANTHI, S. - BAGYARAJ, D. J - SURESH, C. K. (2001): Microbial and enzymatic analysis from sandal root zone soil growing in red sandy loam. Indian Journal of Microbiology 41: 219-221.
MCCARTHY, G. W. - SIDDARAMAPPA, R. - REIGHT, R. J. - CODDLING, E. E. - GAO, G. (1994): Evaluation of coal combustion by products as soil liming materials: their influence on soil pH and enzyme activities. Biology and Fertility of Soils 17: 167-172.
MCLAREN, A. D. (1975): Soil as a system of humus and clay immobilised enzymes. Chemica Scripta 8: 97-99.
NADELHOFFER, K. - BOONE, R. - BOWDEN, R. - CANARY, J. - KAYE, J. - MICKS, P. - RICCA, A. - MCDOWELL, W. - AITKENHEAD, J. (2004): The DIRT experiment. In: Foster, D. R. - Aber, D. J. (ed) Forests in Time. Yale Univ. Press, Michigan.
NANNIPIERI, P. - KANDELER, E. - RUGGIERO, P. (2002): Enzyme activities and microbiological and biochemical processes in soil. In: Burns, R. G. and Dick, R. P. (ed.) Enzymes in the Environment: Activity, Ecology, and Applications. Marcel Dekker, New York. pp. 1-33.
SINSABAUGH, R. L. - MOORHEAD, D. L. (1994): Resource allocation to extracellular enzyme production: a model for nitrogen and phosphorus control of litter decomposition. Soil Biology and Biochemistry 26: 1305-1311.
SINSABAUGH, R. L. - LAUBER, C. L. - WEINTRAUB, M. N. - AHMED, B. - ALLISON, S. D. - CHELSEA, C. - CONTOSTA, A. R. - CUSACK, D. - FREY, S. - GALLO, M. E. - GARTNER, T. B. - HOBBIE, S. E. - HOLLAND, K. - KEELER, B. L. - POWERS, J. S. - STURSOVA, M. - TAKACS-VESBACH, C. - WALDROP, M. P - WALLENSTEIN, M. D. - ZAK, D. R. - ZEGLIN, L. H. (2008): Stoichiometry of soil enzyme activity at global scale. Ecology Letters 11: 1252-1264.
SMITH, S. E. - READ, D. (2008): Mycorrhizal Symbiosis, 3rd edn. Academic Press is an imprint of Elsevier, New York, London, pp. 365.
SULZMAN, E. W. - BRANT, J. B. - BOWDEN, R. D. - LAJTHA, K. (2005): Contribution of aboveground litter, belowground litter, and rhizosphere respiration to total soil CO2 efflux in an old growth coniferous forest. Biogeochemistry 73: 231-256.
TAYLOR, J. P. - WILSON, B. - MILLS, M. S. - BURNS, R. G. (2002): Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biology and Biochemistry 34: 387-401.
THALMANN, A. (1968a): Zur Methodik der Bestimmung der Dehydrogenaseaktivitӓt im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtschaftliche Forschung 21: 249-258.
THALMANN, A. (1968b): Dehydrogenase activity. In: Alef K. and Nannipieri P. (ed) (1995): Methods in Applied Soil Microbiology and Biochemistry. Academic Press Ltd. pp. 228-230. TÓTH, J. A. - LAJTHA, K. - KOTROCZÓ, ZS. - KRAKOMPERGER, ZS. - CALDWEL, B. - BOWDEN, R. D. - PAPP, M. (2007): The effect of climate change on soil organic matter decomposition. Acta Silvatica et Ligniaria Hungarica 3: 75-85.
TÓTH, J. A. - NAGY, P. T. - KRAKOMPERGER, ZS. - VERES, ZS. - KOTROCZÓ, ZS. - KINCSES, S. - FEKETE, I. - PAPP, M. - LAJTHA, K. (2011): Effect of Litter Fall on Soil Nutrient Content and pH, and its Consequences in View of Climate Change (Síkfőkút DIRT Project). Acta Silvatica et Ligniaria Hungarica 7: 75-86.
TREVORS, J. T - MAYFIELD, C. I. - INNISS, W. E. (1982): Measurement of electron transport system (ETS) activity in soil. Microbial Ecology 8: 163-168.
VARGA, CS. - FEKETE, I. - KOTROCZÓ, ZS. - KRAKOMPERGER, ZS. - VINCZE, GY. (2008): Effect of litter amount on soil organic matter (SOM) turnover in Síkfőkút site. Cereal Research Communications 36: 547-550.
WOLIŃSKA, A. - STĘPNIEWSKA, Z. (2012): Dehydrogenase Activity in the Soil Environment, Dehydrogenases. Prof. Rosa Angela Canuto (ed). ISBN: 978-953-307-019-3. InTech. DOI: 10.5772/48294.
XIANG, S. - R. - DOYLE, A. - HOLDEN, P. A. - SCHIMEL, J. P. (2008): Drying and rewetting effects on C and N mineralization and microbial activity in surface and subsurface California grassland soils. Soil Biology and Biochemistry 40: 2281-2289.