Morphometric Characterization of Small Cell Lymphocytic Lymphoma

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Abstract

The morphometry in histopathology is used to characterize cell populations belonging to different tissues and to identify differences in their parameters with prognostic implications. To achieve morphometric examination were selected 6 of 24 cases identified as small cell lymphocytic lymphoma. For each case analysis was done on five fields, for each field measuring the parameters of 20 cells. The studied parameters were for cytoplasm: cytoplasmic area, maximum and minimum cytoplasmic diameter, cytoplasmic perimeter; for nucleus were measured: nuclear area, minimum and maximum nuclear diameter, nuclear perimeter, nuclear contour index, nuclear ellipticity index, nuclear irregularity index. Also the nucleocytoplasmic ratio was calculated in all studied cases. Small cell lymphocytic lymphoma is characterized in morphometric terms having a small cytoplasmic area (average 29.206) and also a small nuclear area (mean 28.939) having a nucleo-cytoplasmic ratio appearance suggestive for adult lymphocyte. A nuclear contour index small value (3.946), ellipticity index value also small (3.521) and small nuclear irregularity index (3.965). Standard deviations, in any of the studied morphometric categories, is around or below 1 suggesting monomorphic cell appearance. These morphometric and microscopic features characterized mainly by a small population of adult lymphocytes, monomorphic, with rounded hipercromic nuclei, dense chromatin, support the framing into indolent lymphoma group in terms of clinical outcome.

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  • 1. Aschie M. (2001). Limfoame maligne gastrointestinale entitati anatomo-clinice si morfologice (1st edition). Constanta Romania: Ed. Muntenia&Leda

  • 2. Ioachim L. H. & Medeiros L.J. (2009). Ioachim’s Lymph Node Pathology (4th Edition). Phyladelphia USA: Ed.Lppincott- Williams&Wilkins

  • 3. Muller-Hermelink H.K. Catovsky D. & Montserrat E. et al. (2001). Chronic lymphocytic leukaemia/small lymphocytic lymphoma. In Jaffe E.S. Harris N.L. Stein H. et al. eds. World Health Organization classification of tumours. Pathology and genetics of tumours of haematopoietic and lymphoid tissues (127-130). Lyon France: IARC Press

  • 4. Gupta D. Lim M.S. & Medeiros L.J. et al. (2000). Small lymphocytic lymphoma with perifollicular marginal zone or interfollicular distribution. Mod Pathol. (11) 1161-1166. PMID:11106071

  • 5. Van Der Valk P. Mosch A. Kurver P.J. & Meijer C.J.L.M. (1983). Morphometric characterization of 52 B cell non-Hodgkin’s lymphomas. JClin Pathol (36) 289-297. 28 october 1982. DOI: 10.1136

  • 6. Swerdlow S.H. Pelstring R.J. & Collins R.D. (1990). Morphometric Analysis of Follicular Center Cell Lymphomas. Am Journal of Pathology. vol 134 (no 4) 953-963. 25 may 1990. PMCID: PMC1877560

  • 7. Tosi P. Leoncini L. Spina D. & Del Vecchio M.T. (1984). Morphometric Nuclear Analysis of Lymphoid Cells in Center Cell Lymphomas and in Reactive Germinal Centers. Am Journal Pathol vol 117 12-17. PMID:6385724 PMCID:PMC1900559

  • 8. Pelstring R.J. & Swerdlow S.H. (1987). Improved nuclear contour indices for lymphoid morphometry. Anal Quant Cytol Histol. 9(6) 469-474. PMID: 3325072

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