DNA Fingerprinting of Sex in Jojoba (Simmondsia chinensis) Grown under the Semi-arid Conditions of Sudan

Abstract

Jojoba cultivation and production face the challenge of establishing ways to identify the sex at early stage of plant growth. The present study was carried out to identify sex of jojoba at the seedling stage under Sudan condition. Two DNA markers, ISSR (UBC807) and RAPD (OPG-5), were used for sex identification of jojoba genotypes: two known male and females genotypes and four unknown genotypes. ISSR marker, UBC807 was successfully amplified a unique male-specific band at 1200 bp, while RAPD marker, OPG-5 could not amplify a unique band within jojoba sex. The result clearly indicates that ISSR-UBC807 marker can be used for sex identification of jojoba at seedlings stage, a finding that could make the commercial cultivation and production of jojoba possible in Sudan.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Modise D. M., In: van der Vossen, H.A.M. and Mkamilo, G.S. (2007). (Eds). PROTA 14: Vegetable oils/Oléagineux. PROTA, Wageningen, Netherlands.

  • 2. Osman H. E. and Abohassan, A. A. (2013). Introducing Jojoba in the Arabian Desert: 1. Agronomic Performance of Nine Jojoba Clones Selected in Makkah Area in Northern and Western Saudi Arabia. International Journal of Theoretical and Applied Sciences, 5(1), 37-46.

  • 3. Benzioni A. (1997). Jojoba [Online] https://hort.purdue.edu/newcrop/CropFactSheets/jojoba.html (browsed on March 17, 2016).

  • 4. Reddy M. P.and Chikara J. (2010). Biotechnology advances in jojoba (Simmondsia chinensis). In: Ramawat KG (ed) Desert plants. Berlin, Springer.

  • 5. Aly M. and Basarir (2012). Biotechnology Approaches and Economic Analysis of Jojoba Natural Products. Biotechnological Production of Plant Secondary Metabolites, 159 – 175.

  • 6. Aftab F., Akram S. and Iqbal J. (2008). Estimation of fixed oils from various explants and in vitro callus cultures of jojoba (Simmondsia chinensis). Pakistan Journal of Botany, 40(4), 1467-1471.

  • 7. El Bassam N. (2010). Handbook of bioenergy crops: a complete reference to species, development and applications. Earthscan, London, UK

  • 8.Wagdy M., Taha S. (2012). Primary Assessment of the Biological Activity of Jojoba Hull Extracts. Life Science Journal, 9(2), 244-253.

  • 9. Al-Qizwini H., Ekbal A. K., Mhaidat N. M. and Maraqa A. (2014). Antioxidant and antimicrobial activities of jordanian Simmondsia chinensis (link) ck schneid. Eur Sci Journal, 10, 229 – 41. http://dx.doi.org/10.19044/esj.2014.v10n27p%25p

  • 10. Mishra A., Mishra A. and Chattopadhyay P. (2011). Herbal Cosmeceuticals for Photoprotection from Ultraviolet B Radiation: A Review. Tropical Journal of Pharmaceutical Research, 10(3), 351-360. http://dx.doi.org/10.4314/tjpr.v10i3.7

  • 11. Abdel-Mageed W., Bayoumi S., Radwan A., Salem-Bekhit M., Abd-Alrahman S. and Basudan, O. (2014). Simmondsia chinensis: A rich source of bioactive flavonoids and lignans. Industrial Crops and Products, 60, 99-103. http://dx.doi.org/10.1016%2Fj.indcrop.2014.06.007

  • 12. Van Wyk B.E. and Wink M. (2004). Medicinal plants of the world. Australian Journal of Medical Herbalism, 16(1), 36-36.

  • 13. Osman M. (1983). A comprehensive follow-up report on: the introduction of jojoba in the Sudan, 13.

  • 14. Agrawal V., Sharma K., Gupta S., Kumar R. and Prasad M. (2007). Identification of sex in Simmondsia chinensis (Jojoba) using RAPD markers. Plant Biotechnology Reports, 1(4), 207-210. https://doi.org/10.1007/s11816-007-0031-6

  • 15. Anita G. and Goyat S. (2015). Marker assisted sex differentiation in dioecious plants. Journal of Pharmacy Research, 9(8), 531-549

  • 16. Sharma K., Agrawal V., Gupta S., Kumar R. and Prasad M. (2008). ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotechnology Reports, 2(4), 239-243. https://doi.org/10.1007/s11816-008-0070-7

  • 17. Ince A., Karaca M., and Onus A. (2010). A reliable gender diagnostic PCR assay for jojoba (Simmondsia chinensis (Link) Schneider). Genetic Resources and Crop Evolution, 57(5), 773-779. https://doi.org/10.1007/s10722-009-9516-1

  • 18. Heikrujam M., Sharma K., Kumar J. and Agrawal V. (2014). Validation of male sex-specific UBC-8071200ISSR marker and its conversion into sequence tagged sites marker in Jojoba: a high precision oil yielding dioecious shrub. Plant Breeding, 133(5), 666-671. https://doi.org/10.1111/pbr.12199

  • 19. Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A. and Allard, R.W.L. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences, 81(24), 8014-8018. https://doi.org/10.1073/pnas.81.24.8014

  • 20. Mohei El Din L., Mohasseb H.A.A., Al-Khateeb A.A., Al-Khateeb S.A., Chowdhury K., El-Shemy H.A. (2017). Identification and sequencing of Date-SRY Gene: A novel tool for sex determination of date palm (Phoenix dactylifera L.). Saudi Journal of Biological Sciences, in press https://doi.org/10.1016/j.sjbs.2017.08.002

  • 21. Urasaki N., Tokumoto M., Tarora K., Ban Y., Kayano T., Tanaka H. (2002). A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.). Theoretical and applied genetics,104(2-3), 281-285. https://doi.org/10.1007/s001220100693

OPEN ACCESS

Journal + Issues

Search