Antibacterial Activity of Bees Gut Lactobacilli against Paenibacillus Larvae In Vitro

Open access


The aim of this study was to evaluate antimicrobial activity of bees gastrointestinal Lactobacillus spp. of against Paenibacillus larvae. Content of the intestinal tract was cultured for isolation of Lactobacillus spp. Gut homogenates were plated on de Man, Rogosa and Sharpe agar (MRS, Oxoid) plates and incubated for 48-72h at 30°C anaerobically. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains Lactobacillus gasseri, L. amylovorus, L. kunkeei, L. fructivorans, Paenibacillus larvae were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus supernatant against two strains of Paenibacillus larvae. The best antimicrobial activity of Lactobacillus against Paenibacillus larvae from gut was found in L. gasseri supernatant. Lesser degree of antimicrobial activity against P. larvae was found in L. kunkeei supernatant. The strongest antibacterial activity against P. larvae CCM 4438 was found in L. gasseri and L. amylovorus and the least antibacterial activity was found in L. fructivorans.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Alvarado I. Margotta J.W. Aoki M.M. Flores F. Agudelo F. Michel G. Elekonich M.M. & Abel- Santos E. (2017). Inhibitory effect of indole analogs against Paenibacillus larvae the causal agent of American foulbrood disease. Journal of Insect Science 17(5):104 1-8

  • 2. Genersch E. (2010). American Foulbrood in honeybees and its causative agent Paenibacillus larvae. Journal of Invertebrate Pathology 103 S10-S19

  • 3. Forsgren E. Stevanovic J. & Fries I. 2008. Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. Veterinary Microbiology 129 342-349

  • 4. Lindström A. Korpela S. & Fries I. (2008). The distribution of Paenibacillus larvae spores in adult bees and honey and larval mortality following the addition of American Foulbrood diseased brood or sporecontaminated honey in honey bee (Apis mellifera) colonies. Journal of Invertebrate Pathology 99 82-86

  • 5. Yue D. Nordhoff M. Wieler L. H. & Genersch E. (2008). Fluorescence in situ hybridization (FISH) analysis of the interactions between honeybee larvae and Paenibacillus larvae the causative agent of American foulbrood of honeybees (Apis mellifera). Environmental Microbiology 10 1612-1620

  • 6. Evans J.D. & Lopez D.L. (2004). Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). Journal of Economic Entomology 97 752-756.

  • 7. Forsgren E. Olofsson T.C. V́asquez A. & Fries I. (2010). Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae. Apidologie 41 99-108

  • 8. Wu M. Sugimura Y. Iwata K. Iwata K. Takaya N. Takamatsu D. Kobayashi M. Taylor D. Kimura K. & Yoshiyama M. (2014). Inhibitory Effect of Gut Bacteria from the Japanese Honey Bee Apis cerana japonica Against Melissococcus plutonius the Causal Agent of European Foulbrood Disease. Journal of

  • Insect Science. 14 129

  • 9. Klūga A. Terentjeva M. Kántor A. Kluz M. Puchalski C. & Kačániová M. (2017). Antibacterial Activity of Melissa officinalis L. Mentha piperita L. Origanum vulgare L. and Malva mauritiana against Bacterial Microflora Isolated from Fish. Advanced Research in Life Sciences 1 75- 80

  • 10. Engel P. & Moran N.A. (2013). The gut microbiota of insects diversity in structure and function. FEMS Microbiology Reviews 37 699-735

  • 11. Engel P. & Moran N.A. (2013). Functional and evolutionary insights into the simple yet specific gut microbiota of the honey bee from metagenomic analysis. Gut Microbes 4 60-65

  • 12. Yoshiyama M. Wu M. Sugimura Y. Takaya N. Kimoto-Nira H. & Suzuki C. (2013). Inhibition of Paenibacillus larvae by lactic acid bacteria isolated from fermented materials Journal of Invertebrate Pathology 112 62-67

  • 13. Jaouani I. Abbassi M.S. Alessandria V. Bouraoui J. Ben Salem R. Kilani H. Mansouri R. Messadi L. & Cocolin L. (2014). High inhibition of Paenibacillus larvae and Listeria monocytogenes by Enterococcus isolated from different sources in Tunisia and identification of their bacteriocin genes. Letters of

  • Applied Microbiology 59 17-25

  • 14. Al-Ghamdi A. Khalid A.K. Ansari M.J. Almasaudi S.B. & Al-Kahtani S. (2017). Effect of gut bacterial isolates from Apis mellifera jemenitica on Paenibacillus larvae infected bee larvae. Saudi Journal of Biological Sciences in press

Journal information
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 483 317 15
PDF Downloads 249 182 7