Evaluation of lysozyme, complement C3, and total protein in different developmental stages of Caspian kutum (Rutilus frisii kutum K.)

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Abstract

In this study, non–specific immune parameters in fertilized eggs, eyed embryos, larvae 10, 25, 50, 60, and 70 days post hatch (DPH), and female broodstock of Caspian kutum, Rutilus frisii kutum (Kamensky), were evaluated. The lysozyme activity, complement C3, and total protein levels were measured with the turbidimetric, immunoturbidimetric, and Bradford methods, respectively. The results showed that lysozyme levels decreased from levels noted in the fertilized eggs until the larvae were 10 days old. Subsequently, significant increases in lysozyme levels were observed until 70 DPH. An increasing trend of complement component C3 was noted from the levels in fertilized eggs to 10 DPH, following which it decreased significantly. Total protein levels differed significantly in early developmental stages of Caspian kutum. The higher values of complement component C3 than of lysozyme in the early life stages could be indicative of the former’s more fundamental role.

References

  • Alexander J.B., Ingram G.A. 1992 – Noncellular nonspecific defence mechanisms of fish – Ann. Rev. Fish. Dis. 2: 249-279.

  • Andrades J.A., Nimini M.E., Becerra J., Eisenstein R., Davis M., Sorgente N. 1996 – Complement proteins are present in developing endochondral bone and may mediate cartilage cell death and vascularization – Exp. Cell. Res. 227: 208-213.

  • Bani A., Vayghan H.A. 2011 – Temporal variations in haematological and biochemical indices of the Caspian kutum, Rutilus frisii kutum – Ichthyol. Res. 58: 126-133.

  • Bradford M.M. 1976 – A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding – Anal. Biochem. 72: 248-254.

  • Brown L.L., Cox W.T., Levine R.P. 1997 – Evidence that the causal agent of bacterial cold water disease Flavobacterium psychrophilum is transmitted within salmonid eggs – Dis. Aquat. Org. 29: 213-218.

  • Cecchini S., Terova G., Caricato G., Saroglia M. 2000 – Lysosome activity in embryos and larvae of sea bass (Dicentrarchus labrax L.), spawned by broodstocks fed with vitamin C enriched diets – Bull. Eur. Assoc. Fish. Pathol. 20: 120-124.

  • Chettri J.K., Raida M.K., Kania P.W., Buchmann K. 2012 – Differential immune response of rainbow trout (Oncorhynchus mykiss) at early developmental stages (larvae and fry) against the bacterial pathogen Yersinia ruckeri – Dev. Comp. Immunol. 36: 463-474.

  • Ellingsen T., Strand C., Monsen E., Břgwald J., Dalmo R.A. 2005 – The ontogeny of complement component C3 in the spotted wolfish (Anarhichas minor Olafsen) – Fish. Shellfish Immunol. 18: 351-358.

  • Ellis A.E. 1999 – Immunity to bacteria in fish – Fish. Shellfish Immunol. 9: 291-308.

  • Engstad R.E., Robertsen B., Frivold E. 1992 – Yeast glucan induces increase in lysozyme and complement–mediated haemolytic activity in Atlantic salmon blood – Fish. Shellfish Immunol. 2:287-297.

  • Gharache M.H., Paighambary S.Y., Golpour A. 2013 – Hematological and biochemical indices of kutum Rutilus frisii kutum, associated with capture methods – Comp. Clin. Path. 4: 979-982.

  • Heidari B., Shabanipour N., Savari A., Yavari V., Hosseini N. 2010 – The oocyte development of Kutum, Rutilus frisii kutum, Kamensky with special emphasis on the zona radiata structure – Anim. Reprod. 6: 465-472.

  • Hermannsdottir R., Johannsdottir J., Smaradottir H., Sigurgisladottir S., Gudmundsdottir B.K., Bjornsdottir R. 2009 – Analysis of effects induced by a pollock protein hydrolysate on early development, innate immunity and the bacterial community structure of first feeding of Atlantic halibut (Hippoglossus hippoglossus L.) larvae – Fish. Shellfish Immunol. 27: 595-602.

  • Holčik J. 1995 – New data on the ecology of kutum, Rutilus frisii (Nordmann, 1840) from the Caspian Sea – Ecol. Freshw. Fish. 4: 175-179.

  • Huttenhuis H.B.T., Grou C.P.O., Taverne-Thiele A.J., Taverne N., Rombout J.H.W.M. 2006 – Carp (Cyprinus carpio L.) innate immune factors are present before hatching – Fish. Shellfish Immunol. 20: 586-596.

  • Jafari M., Kamarudin M.S., Saad C.R., Arshad A., Oryan S., Bahmani M. 2009 – Development of morphology in hatchery–reared Rutilus frisii kutum larvae – Eur. J. Sci. Res. 38: 296-305.

  • Jafari M., Kamarudin M.S., Saad C.R., Arshad A., Oryan S., Guilani M.H.T. 2010 – Embryonic Development of Caspian kutum, Rutilus frisii kutum – J. World. Aquacult. Soc. 41: 378-390.

  • Kudo S. 1991 – Further investigation on enzyme activities and antifungal action of fertilization envelope extract from fish eggs – Zool. Sci. 8: 1079.

  • Kudo S. 1992 – Enzymatic basis for protection of fish embryos by the fertilization envelope – Experientia, 48: 277-281.

  • Lambris J., Lao Z., Pang J., Alsenz J. 1993 – Third component of trout complement, cDNA cloning and conservation of functional sites – J. Immunol. 151: 6123-6134.

  • Lange S., Bambir S., Dodds A.W., Magnadóttir B. 2004a – An immunohistochemical study on complement component C3 in juvenile Atlantic halibut (Hippoglossus hippoglossus L.) – Dev. Comp. Immunol. 28: 593-601.

  • Lange S., Bambir S., Dodds A.W., Magnadóttir B. 2004b – The ontogeny of complement component C3 in Atlantic cod (Gadus morhua L.)–an immunohistochemical study – Fish. Shellfish Immunol. 16: 359-367.

  • Løvoll M., Johnsen H., Boshra H., Bøgwald J., Sunyer J.O., Dalmo R.A. 2007 – The ontogeny and extrahepatic expression of complement factor C3 in Atlantic salmon (Salmo salar) – Fish. Shellfish Immunol. 23: 542-552.

  • Magnadóttir B., Lange S., Gudmundsdottir S., Bøgwald J., Dalmo R.A. 2005 – Ontogeny of humoral immune parameters in fish – Fish. Shellfish Immunol. 19: 429-439.

  • Mauri I., Romero A., Acerete L., MacKenzie S., Roher N., Callol A., Cano A., Alvarez M.C., Tort L. 2011 – Changes in complement responses in Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges – Fish. Shellfish Immunol. 30: 182-188.

  • Mulero I., García–Ayala A., Meseguer J., Mulero V. 2007 – Maternal transfer of immunity and ontogeny of autologous immunocompetence of fish: A minireview – Aquaculture, 268: 244-250.

  • Mulero I., Sepulcre M.P., Fuentes I., García–Alcazar A., Meseguer J., García–Ayala A., Mulero V. 2008 – Vaccination of larvae of the bony fish gilthead seabream reveals a lack of correlation between lymphocyte development and adaptive immunocompetence – Mol. Immunol. 45: 2981-2989.

  • Nakao M., Miura C., Itoh S., Nakahara M., Okumura K., Mutsuro J., Yano T. 2004 – A complement C3 fragment equivalent to mammalian C3d from the common carp (Cyprinus carpio): generation in serum after activation of the alternative pathway and detection of its receptor on the lymphocyte surface – Fish. Shellfish Immunol. 16: 139-149.

  • Rooney I.A., Oglesby T.J., Atkinson J.P. 1993 – Complement in human reproduction: Activation and control – Immunol. Res. 12: 276-294.

  • Takemura A. 1993 – Changes in an immunoglobulin M (IgM)–like protein during larval stages in tilapia, Oreochromis mossambicus – Aquaculture, 115: 233-241.

  • Takemura A. 1996 – Immunohistochemical localization of lysozyme in the prelarvae of tilapia, Oreochromis mossambicus – Fish. Shellfish Immunol. 6:75-77.

  • Takemura A., Takano K. 1995 – Lysozyme in the ovary of tilapia (Oreochromis mossambicus): its purification and some biological properties – Fish. Physiol. Biochem. 14: 415-442.

  • Uribe C., Folch H., Enriquez R., Moran G. 2011 – Innate and adaptive immunity in teleost fish: a review – Vet. Med. 56: 486-503.

  • Wang Z.P., Zhang S.C., Wang G.F., An Y. 2008 – Complement activity in the egg cytosol of zebrafish Danio rerio: evidence for the defense role of maternal complement components – Plos One, 3: e1463.

  • Wang Z., Zhang S., Tong Z., Li L., Wang G. 2009 – Maternal transfer and protective role of the alternative complement components in zebrafish Danio rerio – Plos One, 4: e4498.

  • Wang Z., Zhang S. 2010 – The role of lysozyme and complement in the antibacterial activity of zebrafish (Danio rerio) egg cytosol – Fish. Shellfish Immunol. 29: 773-777.

  • Yousif A., Albright L., Evelyn T. 1991 – Occurrence of lysozyme in the eggs of coho salmon Oncorhynchus kisutch – Dis. Aquat. Organ. 10: 45-49.

  • Yousif A., Albright L., Evelyn T. 1994 – In vitro evidence for the antibacterial role of lysozyme in salmonid eggs – Dis. Aquat. Organ. 19: 15-19.

  • Zapata A.G., Torroba M., Varas A., Jimenez A. 1990 – Immunity in fish larvae – Dev. Biol. Stand. 90: 22-32.

  • Zapata A., Diez B., Cejalvo T., Gutiérrez–de Frías C., Cortés A. 2006 – Ontogeny of the immune system of fish – Fish. Shellfish Immunol. 20: 126-136.

Archives of Polish Fisheries

The Journal of Stanislaw Sakowicz Inland Fisheries Institute

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CiteScore 2016: 0.47

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