The aim of this study was to investigate the effect of seven different levels (0, 25, 50, 100, 200, 400, and 600 mg/kg) of a phytogenic feed additive containing a mixture of essential oils from thyme, black cumin, fennel, anise and rosemary on performance, eggshell quality, bone biomechanical properties and bone mineralization in laying hens. This study consisted of a total of 112, 21-week-old Super Nick laying hens, which were randomly distributed into seven experimental groups. During the 12-week experimental period, each experimental group of four replicates of four birds each was fed with seven treatment diets. Egg weight and egg mass were positively linearly affected by essential oil mixture supplementation. Also, eggshell thickness was increased quadratically by essential oil mixture supplementation. The biomechanical properties and tibia mineral content were adversely affected by essential oil mixture supplementation at the level of 600 and 400 mg/ kg, respectively. These results demonstrated that dietary supplementation with a low or medium concentration of essential oils improved bone parameters, while at high levels were adversely affected in laying hens.
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Akbarian A., Golian A., Kermanshahi H., De Smet S., Michiels J. (2015). Antioxidant enzyme activities, plasma hormone levels and serum metabolites of finishing broiler chickens reared under high ambient temperature and fed lemon and orange peel extracts and Curcuma xanthorrhiza essential oil. J. Anim. Physiol. Anim. Nutr., 99: 150-162.
Ali M.N., Hassan M.S., Abd El- Ghany F.A. (2007). Effect of strain, type of natural antioxidant and sulphate ion on productive, physiological and hatching performance of native laying hens. Int. J. Poult. Sci., 6: 539-554.
Amad A.A., Manner K., Wendler K.R., Neumann K., Zentek J. (2011). Effects ofaphytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poultry Sci., 90: 2811-2816.
Armstrong T.A., Flowers W.L., Spears J.W., Nielsen F.H. (2002). Long-term effects of boron supplementation on reproductive characteristics and bone mechanical properties in gilts. J. Anim. Sci., 80: 154-161.
ASAE (2001). ASAE Standards S459: Shear and Three Point Bending Test of Animal Bone. American Society of Agricultural Engineers, St. Joseph, USA.
Botsoglou N.A., Florou - Paneri P., Botsoglou E., Dotas V., Giannenas I., Koi- dis A., Mitrakos P. (2005). The effect of feeding rosemary, oregano, saffron and α-tocopheryl acetate on performance and oxidative stability of eggs. S. African J. Anim. Sci., 35: 143-151.
Bozkurt M., Alçiçek A., Çabuk M., Küçükyilmaz K., Çatli A.U. (2009). Effect of an herbal essential oil mixture on growth, laying traits, and egg hatching characteristics of broiler breeders. Poultry Sci., 88: 2368-2374.
Bozkurt M., Tokuşoğlu O., Küçükyilmaz K., Akşit H., Çabuk M., Çatli A.U., Seyrek K., Çinar M. (2012 a). Effects of dietary mannan oligosaccharide and herbal essential oil blend supplementation on performance and oxidative stability of eggs and liver in laying hens. Ital. J. Anim. Sci., 11: 223-229.
Bozkurt M., Küçükyilmaz K., Pamukcu M., Çabuk M., Alçiçek A., Çatli A.U. (2012 b). Long-term effects of dietary supplementation with an essential oil mixture on the growth and laying performance of two layer strains. Ital. J. Anim. Sci., 11: 23-28.
Bölükbaşi S.C., Erhan M.K., Kaynar O. (2008). The effect of feeding thyme, sage and rosemary on laying hen performance, cholesterol and some proteins ratio of egg yolk and Escherichia coli count in feces. Arch. Geflügel., 72: 231-237.
Cao P.H., Li F.D., Li F.Y., Ru Y.J., Peron A., Schulze H., Bento H. (2010). Effect of essential oils and feed enzymes on performance and nutrient utilization in broilers fedacorn/soy-based diet. Int. J. Poult. Sci., 9: 749-755.
Capkovicova A., Makova Z., Piesova E., Alves A., Faix S., Faixova Z. (2014). Evaluation of the effects of Salvia officinalis essential oil on plasma biochemistry, gut mucus and quantity of acidic and neutral mucins in the chicken gut. Acta Vet., 64: 138-148.
Cross D.E., Mc Devitt R., Hillman K., Acamovic T. (2007). The effects of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. Brit. Poultry Sci., 48: 496-506.
Çabuk M., Bozkurt M., Alçiçek A., Çatli A.U., Başer K.H.C. (2006). Effect ofadietary essential oil mixture on performance of laying hens in the summer season. S. African J. Anim. Sci., 36: 215-221.
Çabuk M., Eratak S., Alçiçek A., Bozkurt M. (2014). Effects of herbal essential oil mixture asadietary supplement on egg production in quail. Sci. World J., 2014: 1-4.
Denli M., Okan F., Uluocak A.N. (2004). Effect of dietary supplementation of herb essential oils on the growth performance, carcass and intestinal characteristics of quail (Coturnix coturnix japonica). S. African J. Anim. Sci., 34: 174-179.
Hernandez F., Madrid J., Garcia V., Orengo J., Megias M.D. (2004). Influence of two plant extracts on broilers performance, digestibility, and digestive organ size. Poultry Sci., 83: 169-174.
Holder D.P., Bradford M.V. (1979). Relationship of specific gravity of chicken eggs to number of cracked eggs and percent shell. Poultry Sci., 58: 250-251.
Jang I.S., Ko Y.H., Kang S.Y., Lee C.Y. (2007). Effect of commercial essential oils on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Anim. Feed Sci. Technol., 134: 304-315.
Jiang X.R., Awati A., Agazzi A., Vitari F., Ferrari A., Bento H., Bontempo V. (2015). Effects ofablend of essential oils and an enzyme combination on nutrient digestibility, ileum histology and expression of inflammatory mediators in weaned piglets. Animal, 9: 417-426.
Kaya A., Kaya H., Macit M., Çelebi S., Esenbu ğa N., Y örük M.A., Karaoğlu M. (2013). Effects of dietary inclusion of plant extract mixture and copper into layer diets on egg yield and quality, yolk cholesterol and fatty acid composition. J. Fac. Vet. Med., Kafkas Uni., 19: 673-679.
Krause E.L., Ternes W. (1999). Bioavailability of the antioxidative thyme compounds thymol and D-cymene-2,3-diol in eggs. Eur. Food Res. Technol., 209: 140-144.
Lim H.S., Paik I.K. (2003). Effect of supplementary mineral methionine chelates (Zn, Ca and Mn) on performance and eggshell quality of laying hens. Asia. Aust. J. Anim. Sci., 16: 1804-1805.
Lokaewmanee K., Yamauchi K.E., Komori T., Saito K. (2014). Eggshell quality, eggshell structure and small intestinal histology in laying hens fed dietary Pantoea-6® and plant extracts. Ital. J. Anim. Sci., 13: 332-339.
Maenner K., Vahjen W., Simon O. (2011). Studies on the effects of essential-oil-based feed additives on performance, ileal nutrient digestibility, and selected bacterial groups in the gastrointestinal tract of piglets. J. Anim. Sci., 89: 2106-2112.
Mountzouris K.C., Paraskevas V., Tsirtsikos P., Palamidi I., Steiner T., Schatzmayr G., Fegeros K. (2011). Assessment ofaphytogenic feed additive effect on broiler growth performance, nutrient digestibility and caecal microflora composition. Anim. Feed Sci. Technol., 168: 223-231.
Mühlbauer R.C., Lozano A., Palacio S., Reinli A., Felix R. (2003). Common herbs, essential oils, and monoterpenes potently modulate bone metabolism. Bone, 32: 372-380.
Olgun O., Yildiz A.Ö. (2014). Effect of dietary supplementation of essential oils mixture on performance, eggshell quality, hatchability, and mineral excretion in quail breeders. Environ. Sci. Poll. Res., 21: 13434-13439.
Sabbieti M.G., Agas D., Maggi F., Vittori S., Marchetti L. (2011). Molecular mediators involved in Ferulago campestris essential oil effects on osteoblast metabolism. J. Cell. Biochem., 112: 3742-3754.
Skujins S. (1998). Handbook for ICP-AES (Varian-Vista). Ashort guide to Vista Series ICP-AES Operation, version 1.0. Varian Int. AG, Zug, Switzerland.
Super Nick (2012). H&N International. Super Nick white egg layer management guide. Cuxhaven, Germany.
Świątkiewicz S., Koreleski J. (2008). The effect of zinc and manganese source in the diet for laying hens on eggshell and bones quality. Vet. Med., 53: 555-563.
Świątkiewicz S., Arczewska- Włosek A., Józefiak D. (2014). Bones quality indices in laying hens fed diets withahigh level of DDGSand supplemented with selected feed additives. Czech J. Anim. Sci., 59: 61-68.
Taranu I., Marin D.E., Untea A., Janczyk P., Motiu M., Criste R.D. (2012). Effect of dietary natural supplements on immune response and mineral bioavailability in piglets after weaning. Czech J. Anim. Sci., 57: 332-343.
Torki M., Akbari M., Kaviani K. (2015). Single and combined effects of zinc and cinnamon essential oil in diet on productive performance, egg quality traits, and blood parameters of laying hens reared under cold stress condition. Inter. J. Biomet., 59: 1169-1177.
Vali N., Shahin H., Vatankhah M. (2013). Determination of the effects of Cinnamomum zeylanicum Blume and Thymus vulgaris on performance and egg quality of Japanese quail (Coturnix japonica). Res. Op. Anim. Vet. Sci., 3: 280-284.
Wilson J.H., Ruszler P.L. (1996). Effects of dietary boron supplementation on laying hens. Brit. Poultry Sci., 37: 723-729.
Zhou Z.L., Deng Y.F., Tao Q.S., Hu Y.F., Hou J.F. (2009). Effects of Gushukang,a Chinese herbal medicine, on bone characteristics and osteoporosis in laying hens. Poultry Sci., 88: 2342-2345.