15. Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens

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The purpose of this study was to compare the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on the bone development in growing male Ross 308 chickens, assessed on the basis of mechanical, geometric, and histomorphometric parameters of limb bone, and bone zinc content, as well as hormones of somatotropic axis. A total of 80 one-day-old male Ross broiler chickens were randomly allocated to 2 groups of 40 chickens each. The control group was fed with a corn-soybean meal basal diet providing the recommended zinc amount (100 mg×kg-1) from zinc oxide, and the experimental group was supplemented with glycinate chelate providing 25% of the total requirement of the microelement recommended for Ross 308 broiler chicks. The mechanical and histomorphometric parameters and geometry of tibia were determined as well as the serum concentration of growth hormone, IGF-1, osteocalcin and leptin. The serum concentration of Zn, Cu, Ca, Mg, Fe, P and zinc bone content were determined. The results showed that birds fed with the diet supplemented with organic zinc in the amount of 25% of the recommended amount did not exhibit weight and general growth disorders and had an unchanged concentration of growth hormone, leptin, and IGF-1. The serum concentration of Zn, Cu, Ca, Mg, Fe, P did not differ between groups. The contents of zinc detected in bones in the controls and the group supplemented with the organic source did not differ as well. Although tibial mechanics and geometry remained unchanged, histomorphometry revealed a disproportionately large osteoporotic bone. The changes in tibial trabecular bone as a result of the diet supplemented with glycinate chelate only in 25% of the total requirement of the microelement recommended for Ross 308 broiler chicks seems to be insufficient for tibia development.

Andersen O. (2004). Chemical and biological considerations in the treatment of metal intoxications by chelating agents. Mini Rev. Med. Chem., 4: 11-21.

Aviagen(2013). Ross 308 Broiler Nutrition specification. http://www.avigen.com/Ross-308/

Aviagen(2014). Ross 308 Broiler Nutrition specification. Aviagen Incorporated Publishing, Huntsville, AL, USA.

Cao J., Henry P.R., Davis S.R., Cousins R.J., Miles R.D., Littell R.C., Ammer- man C.B. (2002). Relative bioavailability of organic zinc sources based on tissue zinc and metallothionein in chicks fed conventional dietary zinc concentrations. Anim. Feed Sci. Technol., 101: 161-170.

Cook M.E. (2000). Skeletal deformities and the cause: introduction. Poultry Sci., 79: 982-984.

El-Husseiny O.M, Hashish S.M., Ali R.A., Arafa S.A., Abd El-Samee L.D., Ole- my A.A. (2012). Effects of feeding organic zinc, manganese and copper on broiler growth, carcass characteristics, bone quality and mineral content in bone, liver and excreta. Int. J. Poultry Sci., 11: 368-377.

Ferretti J.L., Capozza R.F., Mondelo N., Zanchetta J.R. (1993). Interrelationships between densitometric, geometric and mechanical properties of rat femora: inferences concerning mechanical regulation of bone modelling. J. Bone Min. Res., 8: 1395-1399.

Kwiecień M. (2012). The effect of level and form of Cu and Fe in feed on performance and some metabolic indices of broiler chickens (in Polish). Rozpr. Nauk., UP Lublin, 359 pp.

Kwiecień M., Winiarska- Mieczan A., Zawiślak K., Sroka Sz. (2014). Effect of copper glycinate chelate on biomechanical, morphometric and chemical properties of chicken femur. Ann. Anim. Sci.,14: 127-139.

Marchetti M., Ashmead H. De W., Tossani N., Marchetti S., Ashmead S.D. (2000). Comparison of the rates of vitamin degradation when mixed with metal sulphates or metal amino acid chelates. J. Food Compos. Anal., 13: 875-884.

Mocetti P., Ballanti P., Zalzal S., Silvestrini G., Bonucci E., Nanci A. (2000). Ahistomorphometric, structural and immunocytochemical study of the effects of diet-induced hypocalcemia on bone in growing rats. J. Histochem. Cytochem., 48: 1059-1077.

National Research Council (1994). Nutrient Requirements of Poultry. 9th rev. ed. Natl. Acad. Press, Washington, DC.

Parfitt A.M., Drezner M.K., Glorieux F.H., Kanis J.A., Malluche H., Meunier P.J., Ott S.M., Recker R.R. (1987). Bone histomorphometry: standardization of nomenclature, symbols and units. J. Bone Min. Res., 2: 595-610.

Park S.Y., Birkhold S.G., Kubena L.F, Nisbet D.J., Ricke S.C. (2002). Review on the role of dietary zinc in poultry nutrition, immunity, and reproduction. Biol. Trace Element Res., 101: 147-163.

Paz A., Mendes A.A., Martins M.R.F.B., Fernandes B.C.S., Almeida I.C.L., Mil- bradt E.L., Balog A., Komiyama C.M. (2009). Follow-up of the development of femoral degeneration lesions in broilers. Int. J. Morphol., 27: 571-575.

Paz I.C.L.A., Garcia R.G., Bernardi R.,de Oliveira Seno L.,de Alencar Nääs I., Caldara F.R. (2013). Locomotor problems in broilers reared on new and re-used litter. Italian J. Anim. Sci., 12: 275-279.

Sahraei M., Janmmohamdi H., Taghizadeh A., Cheraghi S. (2012). Effect of different zinc sources on tibia bone morphology and ash content of broiler chickens. Advan. Biol. Res., 6: 128-132.

Seedor G.J., Quartuccio H.A., Thompson D.D. (1991). The bisphosphonate alendronate (MK-217) inhibits bone loss due to ovariectomy in rats. J. Bone Min. Res., 6: 339-346.

Skřivan M., Skřivanová V., Marounek M. (2005). Effects of dietary zinc, iron and copper in layer feed on distribution of these elements in eggs, liver, excreta, soil and herbage. Poultry Sci., 84: 1570-1575.

Srinivasan S., Keilin S.A., Judex S., Bray C., Zernicke R.F., Gross T.S. (2000). Aging- induced osteopenia in avian cortical bone. Bone, 26: 361-365.

Starcher B.C., Hill C.H., Madaras J.G. (1980). Effect of zinc deficiency on bone collagenase and collagen turnover. J. Nutr., 110: 2095-2102.

Śliwa E., Radzki R.P., Puzio I. (1996). Osteochondrosis and tibial dyschondroplasia in chickens, pigs and foals. Med. Weter., 52: 156-159.

Świątkiewicz S., Koreleski J., Zhong D.Q. (2001). The bioavailability of zinc from inorganic and organic sources in broiler chickens as affected by addition of phytase. J. Anim. Feed Sci., 10: 317-328.

Tatara M.R., Brodzki A., Krupski W., Śliwa E., Silmanowicz P., Majcher P., Pier - zynowski S.G., Studziński T. (2005). Effect of alpha-ketoglutarate (AKG) on bone homeostasis and plasma amino acids in turkeys. Poultry Sci., 84: 1604-1609.

Tomaszewska E., Dobrowolski P., Siwicki A.K. (2012 a). Maternal treatment with dexamethasone at minimal therapeutic doses inhibits neonatal bone development inagender-dependent manner. Livest. Sci., 146: 175-182.

Tomaszewska E., Dobrowolski P., Wydrych J. (2012 b). Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone. J. Physiol. Pharmacol., 63: 547-554.

Tomaszewska E., Dobrowolski P., Kwiecień M., Burmańczuk N., Badzian B., Szymańczyk S., Kurlak P. (2014). Alterations of liver histomorphology in relation to copper supplementation in inorganic and organic form in growing rats. Bull. Vet. Inst. Pulawy, 58: 479-486.

Vieira S.L. (2008). Chelated minerals for poultry. Rev. Bras. Ciênc. Avíc., 10: 73-79.

Wang X., Fosmire G.J., Gay C.V., Leach Jr R.M. (2002). Short-term zinc deficiency inhibits chondrocyte proliferation and induces cell apoptosis in the epiphyseal growth plate of young chickens. J. Nutr., 132: 665-673.

Webster A.B. (2004). Welfare implications of avian osteoporosis. Poultry Sci., 83: 184-92.

Whitehead C.C. (2004). Overview of bone biology in the egg-laying hen. Poultry Sci., 83: 193-199.

Ziaie H., Seedor J.G., Quarruccio H.A., Thompson D.D. (2001). The bisphosphonate alendronate (MK-217) inhibits bone loss due to ovariectomy in rats. J. Bone Min. Res., 6: 339-346.

Ziaie H., Bashtani M., Karimi M., Torshizi A., Naeeimipour H., Farhangfar H., Zeinali A. (2011). Effect of antibiotic and its alternatives on morphometric characteristics, mineral content and bone strength of tibia in Ross broiler chickens. Global Vet., 7: 315-322.

Annals of Animal Science

The Journal of National Research Institute of Animal Production

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