14. Effect of Dietary Supplementation of Garlic (Allium Sativum) and Turmeric (Curcuma Longa) on Growth Performance, Carcass Traits, Blood Profile and Oxidative Status in Growing Rabbits

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This study was performed to investigate the effects of dietary supplementation with garlic and turmeric powder as growth promoter agents on performance, carcass traits, serum biochemistry, and antioxidant enzyme activities of growing rabbits. A total of 112 New Zealand White rabbits (NZW) at 5 weeks of age were randomly assigned to seven treatments with four replicates. The dietary treatments consisted of 7 groups as follows; the basal diet as control, phytogenic additives groups were supplemented with 2, 4, and 6 g/kg garlic or turmeric powder added to the basal diet. There were no linear and quadratic differences (P<0.05) in growth performance after garlic or turmeric supplementation at all studied ages. Compared with the control group, supplementation of diets with garlic or turmeric linearly and quadratically elevated immunity biomarkers such as total protein (TP), albumin (AL) and immunoglobulin (IgG) levels and decreased (linearly and quadratically, P<0.05) aspartate aminotransferase (AST), alanine aminotransferase (ALT ), triglyceride (TG) and total cholesterol (TC) levels in rabbit serum. However, TP, AL, globulin (GL), IgG and IgM levels were linearly and quadratically enhanced with increasing turmeric levels versus the control diet. Hepatic superoxide dismutase (SOD), catalase (CAT ) and glutathione peroxidase GSH-Px activities as well as reduced glutathione (GSH) concentrations were linearly and quadratically (P<0.05) improved in garlic or turmeric additives fed groups. While MDA concentration was statistically (linearly, P= 0.022) reduced in comparison with the control group. It could be concluded that garlic or turmeric supplementation (2, 4 and 6 g/kg) did not linearly and quadratically affect growth performance but improved the immunity responses and lowered the lipid profile in blood and lipid peroxidation in liver and increased hepatic antioxidant activity in treated rabbits.

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  • Abdel-Wareth A.A.A. Hammad S. Ahmed H. (2014). Effects of Khaya senegalensis leaves on performance carcass traits haematological and biochemical parameters in rabbits. EXCLI J. 13: 502-512.

  • Acamovic T. Brooker J.D. (2005). Biochemistry of plant secondary metabolites and their effects in animals. P. Nutr. Soc. 64: 403-412.

  • Adibmoradi M. Navidshad B. Seifdavati J. Royan M. (2006). Effect of dietary garlic meal on histological structure of small intestine in broiler chickens. J. Poultry Sci. 43: 378-383.

  • Aebi H. (1984). Catalase in vitro. Method. Enzymol. 105: 121-126.

  • Ahmed S.A. Sedki A.A. Mohamed I.A. (2002). Response of growing rabbits to diets containing black seed garlic or onion as natural feed additives. Egypt. J. Rabbit Sci. 12: 69-83.

  • Alagawany M.M. Farag M.R. Dhama K. (2015). Nutritional and biological effects of turmeric (Curcuma longa) supplementation on performance serum biochemical parameters and oxidative status of broiler chicks exposed to endosulfan in the diets. Asian J. Anim. Vet. Adv. 10: 86-96.

  • Al-Sultan S.I. Gameel A.A. (2004). Histopathological changes in the livers of broiler chicken supplemented with turmeric (Curcuma longa). Int. J. Poultry Sci. 3: 333-336.

  • Amagase H. Petesch B.L. Matsuura Kasuga S. Itakura Y. (2001). Intake of garlic and its bioactive components. J. Nut. 131: 955-962.

  • Amin D. Gustafson S.K. Weinacht J.M. Cornell S.A. Neuenschwander K. Kosmider B. Scotese A.C. Regan J.R. Perrone M.H. (1993). RG12561 (dalvastatin): a novel synthetic inhibitor of HMG-Co Areductase and cholesterol-lowering agent. Pharmacology 46: 13-22.

  • Ankri S. Mirelman D. (1999). Antimicrobial properties of allicin from garlic. Microbes Infect. 1: 125-129.

  • Ao X. Yoo J.S. Lee J.H. Jang H.D. Wang J.P. Zhou T.X. Kim I.H. (2010). Effects offermented garlic powder on production performance egg quality blood profiles and fatty acids composition of egg yolk in laying hens. Asian-Aust. J. Anim. Sci. 23: 786-791.

  • AOAC (1990). Official Methods of Analysis 15th ed. Association of Official Analytical Chemists AOAC Washington D.C.

  • Asai A. Nakagawa K. Miyazawa T. (1999). Antioxidative effects of turmeric rosemary and capsicum extracts on membrane phospholipids peroxidation and liver lipid metabolism in mice. Biosci. Biotech. Bioch. 63: 2118-2122.

  • Ashour E.A. Alagawany M. Reda F.M. Abd El-Hack M.E. (2014). Effect of supplementation of Yucca schidigera to growing rabbits diets on growth performance carcass characteristics serum biochemistry and liver oxidative status. Asian J. Anim. Vet. Adv. 9: 732-742.

  • Balasubramanyam M. Koteswari A.S. Kumar R.F. Monickaraj S.U. Mahes- wari J. Mohan V. (2003). Curcumin-induced inhibition of cellular reactive oxygen species generation: novel therapeutic implications. J. Biosci. 28: 715-721.

  • Basavaraj M. Nagabhushana V. Prakash N. Mallikarjunappa S. Appanna- var M.M. Prashanth W. (2010). Effect of dietary supplementation of Pulvis Curcuma Longa on the voluntary feed intake nutrient digestibility and growth performance of broiler rabbits under summer stress. Vet. World 3: 369-372.

  • Basavaraj M. Nagabhushana V. Prakash N. Appannavar M.M. Wagmare P. Mallikarjunappa S. (2011). Effect of dietary supplementation of Curcuma longa on the biochemical profile and meat characteristics of broiler rabbits under summer stress. Vet. World 4: 15-18.

  • Benzie I.F.F. Wachtel- Galor S. (2011). Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition.

  • Beutler E. Duron O. Kelly B.M. (1963). Improved method for the determination of blood glutathione. J. Lab. Clin. Med. 61: 882-890.

  • Blasco A. Ouhayoun J. Masoero G. (1993). Harmonization of criteria and terminology in rabbit meat research. World Rabbit Sci. 1: 3-10.

  • Chowdhury S.R. Chowdhury S.D. Smith T.K. (2002). Effects of dietary garlic on cholesterol metabolism in laying hens. Poultry Sci. 81: 1856-1862.

  • Dhama K. Latheef S.K. Mani S. Abdul Samad H. Karthik K. Tiwari R. Khan R.U.Alagawany M. Mayada R.F. Alam G.M. Laudadio V. Tufarelli V. (2015). Multiple beneficial applications and modes of action of herbs in poultry health and production - A Review. Int. J. Pharmacol. 11: 152-176.

  • Farag M.R. Alagawany M.M. Dhama K. (2014). Antidotal effect of turmeric (Curcuma longa) against endosulfan-induced cytogenotoxicity and immunotoxicity in broiler chicks. Int. J. Pharmacol. 10: 429-439.

  • Földešiová M. Baláži A. ChrastinováĽ. Chrenek P. (2015). The effect of Curcuma longa dried powder in the diet on weight gain of rabbit does. Slovak J. Anim. Sci. 48: 43-48.

  • Frojdo S. Cozzone D. Vidal H. Pirola L. (2007). Resveratrol isaclass IAphosphoinositide 3-kinase inhibitor. Biochem. J. 406: 511-528.

  • Gbenga E.O. Oluwatoyin E.A. Adebowale N.F. Ayodeji V.A. (2009). Response of broiler chickens in terms of performance and meat quality to garlic (Allium sativum) supplementation. Afr. J. Agr. Res. 4: 511-517.

  • Hafeman D.G. Sunde R.A. Hoekstra W.G. (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rats. J. Nutr. 104: 580-587.

  • Hussein S.N. (2013). Effect of turmeric (Curcuma longa) powder on growth performance carcass traits meat quality and serum biochemical parameters in broilers. J. Adv. Biomed. Pathobiol. Res. 3: 25-32.

  • Jensen C. Engberg R. Jakobsen K. Skibsted L.H. Bertelsen G. (1997). Influence of the oxidative quality of dietary oil on broiler meat storage stability. Meat Sci. 47: 211-222.

  • Kim Y.J. Jin S.K. Yang H.S. (2009). Effect of dietary garlic bulb and husk on the physicochemical properties of chicken meat. Poultry Sci. 88: 398-405.

  • Konjufca V.H. Pesti G.M. Bakalli R.I. (1997). Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poultry Sci. 76: 1264-1271.

  • Lin C.C. Wu S.J. Chang C.H. (2003). Antioxidant activity of Cinnamomum cassia. Phytother. Res. 17: 726-730.

  • Mesa M.D. Ramírez- Tortosa M.C. Aguilera C.M. Gil A. (2000). Nutritional and pharmacological effects of Curcuma longa L. extracts. Recent. Res. Devel. Nut. 3 p. 157.

  • Nouzarian R. Tabeidian S.A. Toghyani M. Ghalamkari G. Toghyani M. (2011). Effect of turmeric powder on performance carcass traits humoral immune responses and serum metabolites in broiler chickens. J. Anim. Feed Sci. 20: 389-400.

  • NRC (1977). Nutrients Requirements of Domestic Animals. Nutrients Requirements of Rabbits. 2nd Edition. National Research Council National Academy of Science. Washington DC. USA.

  • Onu P.N. Aja P.M. (2011). Growth performance and haematological indices of weaned rabbits fed garlic (Allium sativum) and ginger (Zingiber officinale) supplemented diets. Int. J. Food Agr. Vet. Sci. 1: 51-59.

  • Ortserga D.D. Andyar A.C. Anthony T.I. (2008). Growth performance of growing rabbits fed graded levels of garlic (Allium sativum). Proc. 33rd Annual Conference of the Nigerian Society for Animal Production Nigeria pp. 189-191.

  • Ozaki M. Funchinoue S. Teraoda S. Ota K. (1995). The in vivo cytoprotection of ascorbic acid against ischemia/reoxygenation injury of fat liver. Arch. Biochem. Biophys. 318: 439-445.

  • Peiretti P.G. Masoero G. Meineri G. (2011). Effects of replacing palm oil with maize oil and Curcuma longa supplementation on the performance carcass characteristics meat quality and fatty acid profile of the perirenal fat and muscle of growing rabbits. Animal 5: 795-801.

  • Quiles J.L. Dolores M.M. Ramírez- Tortosa C.L. Aguilera C.M. Battino M. GilÁ. Ramírez- Tortosa M.C. (2002). Curcuma longa extract supplementation reduces oxidative stress and attenuates aortic fatty streak development in rabbits. Arterioscler. Thromb. 22: 1225-1231.

  • Qureshi A.A. Din Z.Z. Abuirmeileh N. Burger W.C. Ahmad Y. Elson C.E. (1983). Suppression of avian hepatic lipid metabolism by solvent extracts of garlic: impact on serum lipids. J. Nutr. 113: 1746-1755.

  • Raeesi M. Hoeini-Aliabad S.A. Roofchaee A. Zare S.A. Pirali S. (2010). Effect of periodically use of garlic (Allium sativum) powder on performance and carcass characteristics in broiler chickens. World Acad. Sci. Engin. Technol. 68: 1213-1219.

  • Ramirez-Tortosa M.C. Mesa M.D. Aguilera M.C. Quiles J.L. Baro L. Ramirez- - Tortosa Sa C.L. Martinez-Victoria E. Gil A. (1999). Oral administration ofaturmeric extract inhibits LDLoxidation and has hypocholesterolemic effect in rabbits with experimental atherosclerosis. Arteriosclerosis 147: 371-378.

  • Reddy A.C. Lokesh B.R. (1994). Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol. Cell. Biochem. 137: 1-8.

  • Sher A. Fakhar -ul -Mahmood M. Shah S.N.H. Murtaza S.B.G. (2012). Effect of garlic extract on blood glucose level and lipid profile in normal and alloxan diabetic rabbits. Adv. Clin. Exp. Med. 21: 705-711.

  • Shetty S. Thomas B. Shetty V. Bhandary R. Shetty R.M. (2013). An in-vitro evaluation of the efficacy of garlic extract as an antimicrobial agent on periodontal pathogens: Amicrobiological study. AYU 34: 445-451.

  • Soni K.B. Lahiri M. Chackradeo P. Bhide S.V. Kuttan R. (1997). Protective effect of food additives on aflatoxin-induced mutagenicity and hepatocarcinogenicity. Cancer Lett. 115: 129-133.

  • Sterling S.J. Eagling R.D. (2001). Agronomic and allicin yield of Australian grown garlic (Allium sativum). Acta Hort. 55: 63-73.

  • Tapiero H. Townsend D.M. Tew K.D. (2004). Organosulfur compounds from alliaceae in the prevention of human pathologies. Biomed Pharmacother. 58: 183-193.

  • Toghyani M. Toghyani M. Gheisari A.A. Ghalamkari G. Eghbalsaeid S. (2011). Evaluation of cinnamon and garlic as antibiotic growth promoter substitutions on performance immune responses serum biochemical and haematological parameters in broiler chicks. Livest. Sci. 138: 167-173.

  • Wang J.P. Yoo J.S. Jang H.D. Lee J.H. Cho J.H. Kim I.H. (2011). Effect of dietary fermented garlic by Weissella koreensis powder on growth performance blood characteristics and immune response of pigs challenged with Escherichia coli lipopolysaccharide. J. Anim. Sci. 89: 2123-2131.

  • Wientarsih I. Chakeredza S.ter Meulen U. (2002). Influence of curcuma (Curcuma xanthorrhiza Roxb) on lipid metabolism in rabbits. J. Sci. Food Agr. 82: 1875-1880.

  • Winterbourn C.C. Hawkins R.E. Brain M. Carrell R. (1975). The estimation of red cell superoxide dismutase activity. J. Lab. Clin. Med. 85: 337-341.

  • Yalçin S. Onbasilar E.E. Reisli Z. Yalçin S. (2006). Effect of garlic powder on the performance egg traits and blood parameters of laying hens. J. Sci. Food Agr. 86: 1336-1339.

  • Yan L. Meng Q.W. Ao X. Zhou T.X. Yoo J.S. Kim H.J. Kim I.H. (2011). Effects of fermented garlic powder supplementation on growth performance blood characteristics and meat quality in finishing pigs fed low-nutrient-density diets. Livest. Sci. 137: 255-259.

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