10. The Effect of Chemically-Synthesized Silver Nanoparticles on Performance and the Histology and Microbiological Profile of the Jejunum in Chickens

Katarzyna Ognik 1 , Iwona Sembratowicz 1 , Ewelina Cholewińska 1 , Łukasz Wlazło 2 , Bożena Nowakowicz-Dębek 2 , Radosław Szlązak 3 , and Krzysztof Tutaj 4
  • 1 Department of Biochemistry and Toxicology, Faculty of Biology and Animal Breeding, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
  • 2 Department of Animal Hygiene and Environment, Faculty of Biology and Animal Breeding, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
  • 3 Department of Metrology and Modelling of Agrophysical Processes, Bohdan Dobrzański Institute of Agrophysics of the Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, Poland
  • 4 Chair of Soil Science, Environmental Chemistry and Hydrology, Faculty of Biology and Agriculture, University of Rzeszów, Zelwerowicza 8B, 35-601 Rzeszów, Poland


The aim of the study was to analyse how per os application of hydrocolloids of silver nanoparticles (22 nm) and lipid-coated nanosilver hydrocolloids (5 nm) affect the microbiological status and morphology of the jejunum of broiler chickens and their growth performance. The experiment was conducted on 60 chickens. The first group was the control. The chickens in group II received a silver nanoparticle hydrocolloid (Ag-nano) at a dose of 5 mg/kg b.w./day. The chickens in group III received a lipid-coated nanosilver hydrocolloid (AgL-nano) at a dose of 5 mg/kg b.w./day. Samples of digesta were taken from the jejunum during dissection and the total numbers of fungi, aerobic bacteria and bacteria of the coli group were determined in the samples. Samples of the jejunum were also collected during dissection to determine the length of the villi and depth of the crypts. The silver nanoparticles had no effect on growth performance or the histological picture of the jejunum. An increase was noted in the total number of aerobic mesophilic bacteria and a decrease in the number of coli group bacteria, which are facultative anaerobes, which indicates that the nanosilver had a selective effect on the microflora of the digestive tract in the chickens.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Ahmadi F., Rahimi F. (2011). The effect of different levels of nano silver on performance and retention of silver in edible tissues of broilers. World Appl. Sci., 12: 1-4.

  • Ahmadi J., Mehrdad I., Mahdi C. (2009). Pathological study of intestine and liver in broiler chickens after treatment with different levels of silver nanoparticles. World Appl. Sci. J., 7: 28-32.

  • Ahmadi F., Khah M.M., Javid S., Zarneshan A., Akradi L., Salehifar P. (2013). The effect of dietary silver nanoparticles on performance, immune organs, and lipid serum of broiler chickens during starter period. Inter. J. Biosci., 3: 95-100.

  • Batzri S., Korn E.D. (1973). Single bilayer liposomes prepared without sonication. Biochim. Biophys. Acta, 298: 1015-1019.

  • Braydich-Stolle L., Hussain S., Schlager J.J., Hofmann M.C. (2005). In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol. Sci., 88: 412-419.

  • Choi O., Hu Z. (2008). Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria. Environ. Sci. Technol., 42: 4583-4588.

  • Czaczyk K., Wojciechowska K. (2003). Creating bacterial biofilms - the essence of phenomena and mechanisms of interaction (in Polish). Biotechnologia, 3: 180-192.

  • Egger S., Lehmann R.P., Height M.J., Loessner M.J., Schuppler M. (2009). Antimicrobial properties ofanovel silver-silica nanocomposite material. Appl. Environ. Microbiol., 75: 2973-2976.

  • Euribrid B.V. (1994). Technical Information for Hybro Broilers, Euribrid Poultry Breeding Farm, Boxmeer (The Netherlands), pp. 22.

  • Fondevila M., Herrer R., Casallas M.C., Abecia L., Ducha J.J. (2009). Silver nanoparticles as potential antimicrobial additive for weaned pigs. Anim. Feed Sci. Technol., 150: 259-269.

  • Furowicz A., Boroń - Kaczmarska A., Ferlas M., Czarnomysy-Furowicz A., Po - bucewicz A. (2010). Bacterial biofilm as well as other microbial elements and mechanisms of survival in extreme conditions. Med. Weter., 66: 444-448.

  • Gajbhiye M., Kesharwani J., Ingle A., Gade A., Rai M. (2009). Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomedicine, 5: 382-386.

  • Hussain S.M., Hess K.L., Gearhart J.M., Geiss K.T., Schlager J.J. (2005). In vitro toxicity of nanoparticles in BRL 3Arat liver cells. Toxicol. In Vitro, 19: 975-983.

  • Hussain S.M., Javorina A.K., Schrand A.M., Duhart H.M., Ali S.F., Schlager J.J. (2006). The interaction of manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicol. Sci., 92: 456-463.

  • Local Ethics Commission, Second (2014), University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Resolution No 30/2014, 27. 05.2004.

  • Małaczewska J. (2010). The cytotoxicity of silver nanoparticles. Med. Weter., 66: 833-838.

  • Małaczewska J. (2014). Effect of noble metal nanoparticles on the immune system of animals. Med. Weter., 70: 204-208.

  • Nel A., Xia T., Madler L., Li N. (2006). Toxic potential of materials at the nanolevel. Science, 311: 622-627.

  • Nutrient Requirements for Poultry: (2005). Fourth edition revised and enlarged. Collective work (A. Rutkowski - co-editor), PAN IFi ZZ Jabłonna Poland.

  • Oliveira M.M., Ugarte D., Zanchet D., Zarbin A.J. (2005). Influence of synthetic parameters on the size, structure, and stability of dodecanethiol-stabilized silver nanoparticles. J. Colloid Interf. Sci., 292: 2429-2435.

  • Pineda L., Sawosz E., Lauridsen C., Engberg R.M., Elnif J., Hotowy A., Sa - wosz F., Chwalibóg A. (2012 a). Influence of in ovo injection and subsequent provision of silver nanoparticles on growth performance, microbial profile, and immune status of broiler chickens. Open Acc. Anim. Physiol., 4: 1-8.

  • Pineda L.M., Chwalibog A., Sawosz E., Lauridsen C., Engberg RM., Elnif J., Ho - towy A., Sawosz F., Ali A., Gao Y., Moghaddam H.S. (2012 b). Effect of silver nanoparticles on growth performance, metabolism and microbial profile of broiler chickens. Arch. Anim. Nutr., 66: 416-429.

  • Prabhu S., Poulose E.K. (2012). Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int. Nano. Letters, 2: 1-10.

  • Pyatenko A., Yamaguchi M., Suzuki M. (2007). Synthesis of spherical silver nanoparticles with controllable sizes in aqueous solutions. J. Phys. Chem. C, 111: 7910-7917.

  • Savolainen K., Alenius H., Norppa H., Pylkkänen L., Tuomi T., Kasper G. (2010). Risk assessment of engineered nanomaterials and nanotechnologies -areview. Toxicology, 269: 92-104.

  • Sawosz E., Binek M., Grodzik M., Zieliska M., Sysa P., Szmidt M., Niemiec T., Chwalibog A. (2007). Influence of hydrocolloidal silver nanoparticles on gastrointestinal microflora and morphology of enterocytes of quails. Arch. Anim. Nutr., 61: 444-451.


Journal + Issues