Evaluation of the Nutritional Value of Yellow (Lupinus Luteus) and Blue Lupine (Lupinus Angustifolius) Cultivars as Protein Sources in Rats

Wiesław Sobotka 1 , Maria Stanek 1 ,  and Jacek Bogusz 1
  • 1 Department of Animal Nutrition and Fodder Science, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland


The aim of this study was to determine the nutritional value of protein from the seeds of yellow lupine (Lupinus luteus) and blue lupine (Lupinus angustifolius) cultivars, contained in diets fed to rats. The experimental diets were based on the seeds of three yellow lupine (Mister, Markiz, Taper) or three blue lupine (Sonet, Boruta, Elf) cultivars as the only or main protein source. The nutritional value of the diets was determined based on their chemical composition and alkaloid and oligosaccharide concentrations. Lupine seeds were fed to male Wistar rats with initial body weight of approx. 112 g. Alkaloid concentrations in yellow lupine and blue lupine seeds were 0.07-0.09 g kg-1 DM and 0.26-0.39 g kg-1 DM, respectively. Oligosaccharide concentrations in yellow and blue lupine seeds were 108.0-108.9 and 65.4-67.6 g kg-1 DM, respectively. The inclusion of lupine seeds in rat diets increased fecal and urinary nitrogen losses, and decreased nitrogen retention by approx. 10%. The diets based on yellow or blue lupine seeds were characterized by lower nitrogen digestibility and lower biological value of protein than the control diet containing casein.

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  • AOAC (2003). Official methods of analysis of the Association of Official Analytical Chemists. 17th Ed., AOAC, Arlington, Virginia.

  • Benevenga N.J., Gahl M.J., Blemings K.P. (1993). Role of protein synthesis in amino acid catabolism. J. Nutr., 123: 332-336.

  • Campbell B.I., La Bounty P.M., Roberts M. (2004). The ergogenic potential of arginine. J. Int. Soc. Sports Nutr., 1: 35-38.

  • Champ M.M.J. (2002). Non-nutrient bioactive substances of pulses. Brit. J. Nutr., 88: 307-319.

  • Chew P.G., Casey A.J., Johnson S.K. (2003). Protein quality and physico-functionality of Australian sweet lupin (Lupinus angustifolius cv. Gungurru) protein concentrates prepared by isoelectric precipitation or ultrafiltration. Food Chem., 83: 575-583.

  • De Cortes-Sánchez M., Altares P., Pedrosa M.M., Burbano C., Cuadrado C., Goyoaga C., Muzquiz M., Jiménez- Martínez C., Dávila-Ortiz G. (2005). Alkaloid variation during germination in different lupin species. Food Chem., 90: 347-355.

  • Dijkstra D.S., Linnemann A.R.,van Boekel T.A. (2003). Towards sustainable production of protein-rich foods: appraisal of eight crops for Western Europe. Part II: Analysis of the technological aspects of the production chain. Critical Reviews. Food Sci. Nutr., 43: 481-506.

  • Dixon R.M., Hosking B.J. (1992). Nutritional value of grain legumes for ruminants. Nutr. Res. Rev., 5: 19-43.

  • Eggum B.O., Tomes G., Beames R.M., Datta F.U. (1993). Protein and energy evaluation with rats of seed from 11 lupin cultivars. Anim. Feed Sci. Tech., 43: 109-119.

  • Flis M., Sobotka W., Zduńczyk Z. (1998). Replacement of soyabean meal by white lupine cv. Bardo seeds and the effectiveness ofβ-glucanase and xylanase in growing-finishing pig diets. J. Anim. Feed Sci., 7: 301-312.

  • Fooks L.J., Gibson G.R. (2002). Probiotics as modulators of the gut flora. Brit. J. Nutr., 88: 39-49.

  • Gdala J., Buraczewska L. (1996). Chemical composition and carbohydrate content of seeds from several lupin species. J. Anim. Feed Sci., 5: 403-416.

  • Guillon F., Champ M.M.J. (2002). Carbohydrate fractions of legumes: uses in human nutrition and potential for health. Brit. J. Nutr., 88: 293-306.

  • Gulewicz P., Ciesiołka D., Frias J., Vidal- Valverede C., Frejnagel S., Tro- janowska K., Gulewicz K. (2000). Simple method of isolation and purification of α-galactosides from legumes. J. Agr. Food Chem., 48: 3120-3123.

  • Gulewicz P., Martinez-Villaluenga C., Frias J., Ciesiołka D., Gulewicz K., Vi - dal -Valverde C. (2008). Effect of germination on the protein fraction composition of different lupin seeds. Food Chem., 107: 830-844.

  • Hanczakowska E., Świątkiewicz M. (2014). Legume seeds and rapeseed press cake as replacers of soybean meal in feed for fattening pigs. Ann. Anim. Sci., 14: 921-934.

  • Jezierny D., Mosenthin R., Bauer E. (2010). The use of grain legumes asaprotein source in pig nutrition. Anim. Feed Sci. Tech., 5: 111-128.

  • Juśkiewicz J., Godycka-Kłos I., Matusevicius P., Zduńczyk Z., Juśkiewicz. M. (2006). Influence of pea and lupin oligosaccharides on caecal short-chain fatty acids production and nitrogen excretion patterns in rats. Pol. J. Food Nutr. Sci., 15/56: 77-82.

  • Krawczyk M., Mikulski D., Przywitowski M., Jankowski J. (2015). The effect of dietary yellow lupine (L. luteus cv. Baryt) on growth performance, carcass characteristics, meat quality and selected serum parameters of turkeys. J. Anim. Feed Sci., 24: 61-70.

  • Lehmann H., Bergner H. (1968). Vergleich der Kot- und Harn-N-Trennung in 2 verschiedenen Ratten-Stoffwechselkäfigsystemen. Arch. Tierernahr., 18: 114-120.

  • Lubowicki R., Petkov K., Kotlarz A., Jaskowska I. (2000). Evaluation of protein quality in seeds of some legume varieties. Ann. Anim. Sci., 27: 247-260.

  • Maknickiene Z., Asakaviciute R., Baksiene E., Razukas A. (2013). Alkaloid content variations in Lupinus luteus L. and Lupinus angustifolius L. Arch. Biol. Sci. Belgrade, 65: 107-112.

  • Martinez-Villaluenga C., Frias J., Vidal- Valverde C. (2006). Functional lupin seeds (Lupinus albus L. and Lupinus luteus L.) after extraction ofα-galactosides. Food Chem., 98: 291-299.

  • Mitchell H.H., Block R.J. (1946). Some relationships between the amino acid content of proteins and their nutritive value for the rat. J. Biol. Chem., 163: 599-607.

  • Molving L., Tabe L.M., Eggum B.O., Moore A.E., Craig S., Spencer D., Hig- gins T.J.V. (1997). Enhanced methionine levels and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressingasunflower seed albumin gene. Proc. Natl. Acad. Sci. USA, 94: 8393-8398.

  • Mori M., Gotoh T. (2000). Regulation of nitric oxide production by arginine metabolic enzymes. Biochem. Biophys. Res. Commun., 275: 715-719.

  • National Research Council (1995). Nutrient Requirements of Laboratory Animals, 4th ed. National Academy of Sciences, Washington, DC.

  • Oser B.L. (1959). An integrated essential amino acid index for predicting the biological value of proteins. In: Amino Acid Nutrition. Academic Press, New York, pp. 295-311.

  • Papoti V., Makri E., Papalamprou E., Drakos A., Mandalon D., Doxastakis G.,Kiosseoglou V. (2005). Utilization of lupin seed protein isolates for the production of lupin biscuits and lupin pasta. Proc. Final Conference of Healthy-Profood. Milan, pp. 77-88.

  • Rubio L.A., Grant G. Scislowski , P.W., Brown D., Bardocz S., Pusztai A. (1995). The utilization of lupin (Lupinus angustifolius) and faba bean globulins by rats is poorer than of soybean globulins or lactalbumin but the nutritional value of lupin seed meal is lower only than that of lactalbumin. J. Nutr., 125: 2145-2155.

  • Schumacher H., Paulsen H. M., Gau A. E, Link W., Jürgens H. U., Sass O., Diet erich R. (2011). Seed protein amino acid composition of important local grain legumes Lupinus angustifolius L., Lupinus luteus L., Pisum sativum L. and Vicia faba L. Plant Breeding, 130: 156-164.

  • Smulikowska S., Konieczka P., Czerwinski J., Mieczkowska A., Jankowiak J. (2014). Feeding broiler chickens with practical diets containing lupin seeds (L. angustifolius or L. luteus): effect of incorporation level and mannanase supplementation on growth performance, digesta viscosity, microbial fermentation and gut morphology. J. Anim. Feed Sci., 23: 64-72.

  • Sobotka W., Stanek M., Bogusz J., Matusevicius P. (2013). The effect of oligosaccharides and alkaloids contained in yellow and blue lupine seeds on feed intake, body weight and fermentation processes in the cecum of rats. Vet. Med. Zoot., 63: 63-70.

  • Stanek M., Bogusz J., Sobotka W., Bieniaszewski T. (2012). Nutritional value of blue lupine seeds (in Polish). Fragm. Agron., 29: 160-166.

  • Sudzinová J., Chrenková M., Čereš ňáková Z., Mlyneková Z., Tomíková A. (2009). Effect of lupine and field pea on growth parameters and nitrogen balance in rats. Slovak J. Anim. Sci., 42: 107-110.

  • Sujak A., Kotlarz A., Strobel W. (2006). Composition and nutritional evaluation of several lupin seeds. Food Chem., 98: 711-719.

  • Tong B.C., Barbul A. (2004). Cellular and physiological effects of arginine. Mini Rev. Med. Chem., 4: 823-832.

  • Topping D.L., Clifton P.M. (2001). Short chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev., 81: 1031-1064.

  • Van Soest P.J. (1973). Collaborative study of acid detergent fiber and lignin. J. Assoc. Offic. Anal. Chem., 56: 781-784.

  • Van Soest P.J., Wine R.H. (1967). Use of detergents in the analysis of fibrous feed. IV. Determination of plant cell wall constituents. J. Assoc. Offic. Anal. Chem., 50: 50-55.

  • Younes H., Garleb K., Behr S., Remesy C., Demigne C. (1995). Fermentable fibers or oligosaccharides reduce urinary nitrogen excretion by increasing urea disposal in the rat cecum. J. Nutr., 125: 1010-1016.

  • Wäsche A., Müller K., Knauf U. (2001). New processing of lupin protein isolates and functional properties. Nahrung/Food., 45: 393-395.

  • Wink M., Meissner C., Witte L. (1995). Patterns of quinolizidine alkaloids in 56 species of the genus lupinus. Phytochem., 38: 139-153.

  • Wróblewska M., Zduńczyk Z., Juśkiewicz J., Gulewicz K. (2004). Influence of oligosaccharide extracts from pea and lupin seeds on cæcal fermentation and nitrogen excretion patterns in rats. Wild and cultivated lupins from the Tropics to the Poles. Proc. 10th International Lupin Conference, Laugarvatn, Iceland, 19-24.06.2002, pp. 347-348.

  • Wu G., Morris S.M. (1998). Arginine metabolism: nitric oxide and beyond. Biochem. J., 336: 1-17.

  • Zduńczyk Z., Juśkiewicz J., Frejnagel S., Gulewicz K. (1998). Influence of alkaloids and oligosaccharides from white lupin seeds on utilization of diets by rats and absorption of nutrients in the small intestine. Anim. Feed Sci. Tech., 72: 143-154.

  • Zduńczyk Z., Jankowski J., Mikulski D., Mikulska M., Lamparski G., Slomins- ki B.A., Juśkiewicz J. (2014). Growth performance, gastrointestinal function and meat quality in growing-finishing turkeys fed diets with different levels of yellow lupine (L. luteus) seeds. Arch. Anim. Nutr., 68: 211-226.


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