The purpose of this study was to evaluate the growth performance and intestinal microflora population of broilers fed diets with aged brown rice (ABR) that had been stored for 3 years. The ABR was the material chosen to analyse the grain quality, which included the physical properties as well as nutrient analysis to evaluate its applicability in broiler diet. The germination rate, pH value, whiteness value of grains and fatty acid content of aged rice are significantly reduced during storage (P<0.05), while the nutritional value and content of essential amino acids do not differ from those of fresh brown rice (P>0.05). A total of five hundred 1-day-old broilers (Ross 308) were evenly divided by gender and randomly allocated into 5 groups (with 4 replicates of 25 birds), including: 1) control (corn-soybean meal), 2) 25% of corn replaced with 25% of ABR (ABR 25), 3) 50% of corn replaced with 50% of ABR (ABR 50), 4) 75% of corn replaced with 75% of ABR (ABR 75), 5) 100% of ABR replaced with yellow corn (ABR 100), respectively, in order to evaluate growth performance as well as intestinal microflora population of broilers fed ABR. The diets were formulated to contain similar levels of the main nutrient requirements of broilers. The results indicated that the replacement of ABR for corn in feed had no significant impact on the growth traits of broilers (P>0.05). The pH value of ileal digesta decreased as the replacement of ABR increased at the age of 21 days (P<0.05). The volatile fatty acids of ileal digesta of the ABR 75 and ABR 100 groups were significantly lower than those of the control group at the age of 21 days (P<0.05), but not caecum. There was no difference with lactic acid bacteria and coliform counts in ileum and caecum, respectively, of broilers among the groups during the entire feeding period (P>0.05). In conclusion, this study found that ABR may replace 100% of yellow corn and did not influence growth performance of broilers, thus providing an alternative for the use of stored rice in broiler diets.
AOAC (1998). Official methods of analysis (15th ed.). Association of Official Analytical Chemists. Washington. DC.
Chen T.F., Chen C.L. (2003). Analysing the freshness of intact rice grains by colour determination of peroxidase activity. J. Sci. Food Agric., 83: 1214-1218.
Cherrington C.A., Hinton M., Chopra I. (1990). Effect of short-chain organic acids on macromolecular synthesis in Escherichia coli. J. Appl. Bacteriol., 68: 69-74
CummingsJ.H., Macfarlane G.T. (1991). A review: the control Czech A., Pastuszak J., Kusior G. (2014). Effects of increased content of animal protein in feed mixtures based on extruded rice on rearing performance and blood parameters of piglets. Ann. Anim. Sci., 14: 117-126.
González - Alvarado J.M., Jiménez - Moreno E., Valencia D.G., LázaroR., Mateos G.G. (2008). Effects of fiber source and heat processing of the cereal on the development and pH of the gastrointestinal tract of broilers fed diets based on corn or rice. Poultry Sci., 87: 1779-1795.
Jadhao S.B., Chandramoni A.S., Tiwari C.M., Khan M.Y. (1999). Efficiency of utilisation of energy from maize- and broken rice based diets in old White Leghorn and Rhode Island Red laying hens. Br. Poultry Sci., 40: 275-283.
Lee T.T., Chang C.C., Juang R.S., Chen R.B, Yang S.Y., Chu L.W., Wang S . R., Wang C.S., Tseng T.H., Chen L.J., Yu B. (2010). Porcine lactoferrin expression in transgenic rice and its effects as a feed additive on early weaned piglets. J. Agric. Food Chem., 58: 5166-5173.
Lee T.T., HuangY.F., Chiang C.C., ChungT.K., ChiouP.W.S., Yu B. (2011). Starch characteristics and their influences on in vitro and pig prececal starch digestion. J. Agric. Food Chem., 59: 7353-7359.
Leeson S., Namkung H., Antongiovanni M., L e e E.H. (2005). Effect of butyric acid on the performance and carcass yield of broiler chickens. Poultry Sci. 84: 1418-1422.
LiD.F., Zhang D.F., Piao X.S., Han I.K., YangC.J., Li J.B., Lee J.H. (2002). Effects of replacing maize with Chinese brown rice on growth performance and apparent fecal digestibility of nutrients in weanling pigs. Asian-Aust. J. Anim. Sci., 15: 1191-1197.
Li J., Li D., Yin Y.L., Piao X.S., He J.H., Chen G.P., ShuJ.C. (2004). Performance, nutrient digestibility and intestinal disaccharidase activity of weaner/grower pigs given diets containing extruded Chinese stored brown rice with exogenous enzyme supplements. Anim. Sci., 79: 429-438.
Lii L.J., Wang C.Y., L u r H.S. (1999). A novel means of analyzing the soluble acidity of rice grains. Crop Sci., 39: 1160-1164.
Macfarlane G.T., Gibson G.R. (1995). Microbiological aspects of short chain fatty acid production in the large bowel. In: Physiological and Clinical Aspects of Short Chain Fatty Acid Metabolism. Cambridge University Press, Cambridge, pp. 119-132.
Mateos G.G., Lopez E., Latorre M.A., Vicente B., LazaroR.P. (2007). The effect of inclusion of oat hulls in piglet diets based on raw or cooked rice and maize. Anim. Feed Sci. Technol., 135: 100-112.
OlivaresM., Díaz- Popero M.P., Gómez N., Sierra S., Lara - Villoslada F., MartínR., Rodríguez J.M., Xaus J. (2006). Dietary deprivation of fermented foods causes a fall in innate immune response: Lactic acid bacteria can counteract the immunological effect of this deprivation. J. Dairy Res., 22: 1-7.
Parker D.S. (1976). The measurement of production rates of volatile fatty acids in the caecum of the conscious rabbit. Br. J. Nutr., 36: 61-70.
Piao X.S., Li D., Han I.K., Chen Y., Lee J.H., Wang D.Y., LiJ.B., Zhang D.F. (2002). Evaluation of Chinese brown rice as an alternative energy source. Asian-Aust. J. Anim. Sci., 15: 89-93.
Snel J.H., Harmsen J.M., ven de Wielen P.W.J.J., Williams B.A. (2002). Dietary strategies to influence the gastrointestinal microflora of young animals, and its potential to improve intestinal health. In: Nutrition and health of the gastrointestinal tract, Blok M.C. (ed.). Wageningen Academic Publishers, Wageningen, Netherlands, pp. 37-69.
Tamaki M., Tashiro T., Ishikawa M., Ebata M. (1993). Physico-ecological studies on quality formation of rice kernel: IV. Effect of storage on eating quality of rice. Jpn. J. Crop Sci., 62: 540-546.
Thompson J.L., Hinton M. (1997). Antibacterial activity of formic and propionic acids in the diet of hens on salmonellas in the crop. Br. Poultry Sci., 38: 59-63.
Van Immerseel F., De Buck J., Meulemans G., Pasmans F., Velge P., Bottreau E., Haesebrouck F., Ducatelle R. (2003). Invasion of Salmonella enteritidis in avian intestinal epithelial cells in vitro is influenced by short-chain fatty acids. Inter. J. Food Micro., 85: 237-248.
Vicente B., Valencia D.G., Pérez - Serrano M., Lázaro R., Mateos G.G. (2008). The effects of feeding rice in substitution of corn and the degree of starch gelatinization of rice on the digestibility of dietary components and productive performance of young pigs. J. Anim. Sci., 86: 119-126.
Zhang D.F., Li D.F., Piao X.S., Han I.K., Yang C.J., Dai J.G., Li J.B. (2002). Effects of replacing maize with brown rice or brown rice with enzyme on growth performance and nutrient digestibility in growing pigs. Asian-Aust. J. Anim. Sci., 15: 1334-1340