Effects of species-specific probiotic addition to milk replacer on calf health and performance during the first month of life

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The aim of this study was to evaluate the effects of the administration of a species-specific probiotic (Lactobacillus animalis SB310, Lactobacillus paracasei subsp. paracasei SB137 and Bacillus coagulans SB117 in a 30:35:35 ratio, respectively; 1.8 × 1010 CFU/g of powder) on gut microbial balance, immune response and growth performance of Holstein female calves during the first month of life. Twenty-two calves were divided into two experimental groups from 2 to 28 days of life: control (C), fed with milk replacer and concentrate as a basal diet, and treatment (T), fed C diet plus 1 g/calf/day of probiotic powder for the first month of age. Faecal and blood samples were individually collected and analysed weekly. Individual faecal score was recorded daily and general health score was calculated at the end of the trial. Cell-mediated immune response was evaluated by skin test at 7 and 28 days of life. Milk replacer and concentrate intake were recorded daily, while body weight and biometrical parameters were recorded at 2, 8, 14, 21 and 28 days of life, thus average daily gain and feed conversion rate were calculated. During the first week of treatment, lower blood eosinophil percentage (0.05% vs. 0.22%; P≤0.01) was found in T group, while basophils were higher in T than C group at the end of the trial (0.21% vs. 0.16%; P≤0.05). Higher faecal lactic acid bacteria (LAB)/E. coli ratio on day 28 of life (3.73 log CFU/g vs. 2.02 log CFU/g; P≤0.05) and lower incidence of diarrhoea were found in the treated group (63.30% vs. 70.71%; P=0.05). Body weight (48.92 kg vs. 46.92 kg; P≤0.05), total concentrate intake (14.77 kg vs. 12.56 kg on dry matter basis; P≤0.05), and heart girth (81.16 cm vs. 78.49 cm; P≤0.05) were significantly higher in T group. The administration of the probiotic during the first month of life improved gut microbiota and increased the growth performance and some biometric parameters of calves.


  • Abada E.A. (2008). Isolation and characterization of an antimicrobial compound from Bacillus coagulans. Anim. Cells Syst., 12: 41-46.

  • Abe F.N., Ishibashi X., Shimamura S. (1995). Effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglets. J. Dairy Sci., 78: 2838-2846.

  • Abu- Tarboush H.M., Al - Saiady M.Y., El - Din A.H.K. (1996). Evaluation of diet containing Lactobacilli on performance, fecal coliform, and Lactobacilli of young dairy calves. Anim. Feed Sci. Tech., 57: 39-49.

  • Agazzi A., Cattaneo D., Dell ’ Orto V., Moroni P., Bonizzi L., Pasotto D., Savoi-ni G. (2004). Effect of administration of fish oil on aspects of cell-mediated immune response in periparturient dairy goats. Small Ruminant Res., 55: 77-83.

  • Agazzi A., Cigalino G., Mancin G., Savoini G., Dell ’ Orto V. (2007). Effects of dietary humates on growth and an aspect of cell-mediated immune response in newborn kids. Small Ruminant Res., 72: 242-245.

  • Al - Saiady M.Y. (2010). Effect of probiotic bacteria on immunoglobulin Gconcentration and other blood components of newborn calves. J. Anim. Vet. Adv., 9: 604-609.

  • Bakhshi N., Ghorbani G.R., Rahmani H.R., Samie A. (2006). Effect of probiotic and milk feeding frequency on performance of dairy Holstein calves. Int. J. Dairy Sci., 1, 2: 113-119.

  • Bakr H.A., Said E.M., El - Tawab A., Hassan M.S. (2009). The impact of probiotic (Biovet) on some clinical, hematological and biochemical parameters in buffalo-calves. Vet. Med. J., 19: 1-10.

  • Chaucheyras- Durand F., Durand H. (2010). Probiotics in animal nutrition and health. Beneficial Microbes. Wageningen Academy Publishers, 1, 1: 3-9.

  • Corcionivoschi N., Drinceanu D., Pop I., Stack M., Stef D., Julean L. (2010). The effect of probiotics on animal health. Anim. Sci. Biotechnol., 43: 35-41.

  • Davis C.L., Drackley J.K. (2002). Desarrollo, nutricionymanejo del ternero joven. Intermedica, Buenos Aires.

  • Endo A., Futagawa - Endo Y., Dicks L.M.T. (2010). Diversity of Lactobacillus and Bifidobacterium in feces of herbivores, omnivores and carnivores. Anaerobe, 16: 590-596.

  • Fleige S., Prei Binger H.H.D., Meyer Pfaffl M.W. (2008). The immunomodulatory effect of lactulose on Enterococcus faecium fed preruminant calves. J. Anim. Sci., 87: 1731-1738.

  • Frizzo L.S., Soto L.P., Zbrun M.V., Bertozzi E., Sequeira G., Armesto R.R., Ros-mini M.R. (2010). Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder. Anim. Feed Sci. Tech., 157: 159-167.

  • Frizzo L.S., Soto L.P., Bertozzi E., Zbrun M.V., Signorini M.L., Sequeira G., Ro -driguez R., Armesto R.R., Rosmini M.R. (2011 a). Intestinal populations of Lactobacilli and Coliforms after in vivo Salmonella dublin challenge and their relationship with microbial translocation in calves supplemented with lactic acid bacteria and lactose. Anim. Feed Sci. Tech., 170: 12-20.

  • Frizzo L.S., Zbrun M.V., Soto L.P., Signorini M.L. (2011 b). Effects of probiotics on growth performance in young calves: Ameta-analysis of randomized controlled trials. Anim. Feed Sci. Tech., 169: 147-156.

  • Gaggìa F., Mattarelli P., Biavati B. (2010). Probiotics and prebiotics in animal feeding for safe food production. Int. J. Food Microbiol., 141: S15-S28.

  • Hasunuma T.K., Kawashima H., Nakayama H., Murakami T., Kanagawa H., Ishii T., Akiyama K., Yasuda K., Terada F., Kushibiki S. (2011). Effect of cellooligosaccharide or synbiotic feeding on growth performance, fecal condition and hormone concentrations in Holstein calves. Anim. Sci. J., 82: 543-548.

  • Hoffman P.C. (1997). Optimum body size of Holstein replacement heifers. J. Anim. Sci., 75: 836-845.

  • Huang J.M., La Ragione R.M., Nunez A., Cutting S.M. (2008). Immunostimulatory activity of Bacillus spores. FEMS Immunol. Med. Mic., 53: 195-203.

  • Hyronimus B., Le Marrec C., Urdaci M.C. (1998). Coagulin,abacteriocin-like inhibitory substance produced by Bacillus coagulans I4. J. Appl. Microbiol., 85: 42-50.

  • Kawakami S.I., Yamada Y., Nakanishi N., Cai Y. (2010). Feeding of lactic acid bacteria and yeast on growth and diarrhea of Holstein calves. J. Anim. Vet. Adv., 9: 1112-1114.

  • Kim M.K., Lee H.G., Park J.A., Kang S.K., Choi Y.J. (2011). Effect of feeding direct-fed microbial as an alternative to antibiotics for the prophylaxis of calf diarrhea in Holstein calves. Asian. Austral. J. Anim., 23: 643-649.

  • Lacetera N., Bernabucci U., Ronchi B., Nardone A. (1999). The effects of injectable sodium selenite on immune function and milk production in Sardinian sheep receiving adequate dietary selenium. Vet. Res., 30: 363-370.

  • Lalles J.P., Bosi P., Smidt H., Stokes C.R. (2007). Nutritional management of gut health in pigs around weaning. Proc. Nutr. Soc., 66: 260-268.

  • Le Jeune J.T., Wetzel A.N. (2007). Preharvest control of Escherichia coli O157 in cattle. J. Anim. Sci., 85: 73-80.

  • Le Marrec C., Hyronimus B., Bressollier P., Verneuil B., Urdaci M.C. (2000). Biochemical and genetic characterization of coagulin,anew antilisterial bacteriocin in the pediocin family of bacteriocins, produced by Bacillus coagulans I4. Appl. Environ. Microbiol., 66: 5213-5220.

  • Lucas A.S., Swecker W.S., Lindsay D.S., Scaglia G., Elvinger F.C., Zajac A.M. (2007). The effect of weaning method on coccidial infections in beef calves. Vet. Parasitol., 145: 228-233.

  • Masucci F., Rosa G.D., Grasso F., Napolitano F., Esposito G., Francia A.D. (2011). Performance and immune response of buffalo calves supplemented with probiotic. Livest. Sci., 137: 24-30.

  • Matsumoto D., Takagi M., Hasunuma H., Fushimi Y., Ohtani M., Sato T., Oka-moto K., Shahada F., Tanaka T., Deguchi E. (2009). Effects of oral administration of difructose anhydride IIIon selected health and blood parameters of group-housed Japanese Black calves during the preweaning period. Asian-Aust. J. Anim., 22: 1640-1647.

  • Mead G.C., Impey C.S. (1986). Current progress in reducing Salmonella colonization of poultry by ‘competitive exclusion’. J. Appl. Bacteriol. Symp. (Suppl. 61): 67-75.

  • Mohri M., Sharifi K., Eidi S. (2007). Hematology and serum biochemistry of Holstein milk calves: age related changes and comparison with blood composition in adults. Res. Vet. Sci., 83: 30-39.

  • Morrison S.J., Dawson S., Carson A.F. (2010). The effects of mannan oligosaccharide and Streptococcus faecium addition to milk replacer on calf health and performance. Livest. Sci., 131: 292-296.

  • Nagashima K., Yasokawa D., Abe K., Nakagawa R., Kitamura T., Miura T., Koga -wa S. (2010). Effect ofa Lactobacillus species on incidence of diarrhea in calves and change of the microflora associated with growth. Biosci. Microflora., 29, 2: 97-110.

  • Riddell J.B., Gallegos A.J., Harmon D.L., Mc Leod K.R. (2010). Addition ofa Bacillus based probiotic to the diet of preruminant calves: influence on growth, health, and blood parameters. Int. J. Appl. Res. Vet. M., 8: 78-85.

  • Ripamonti B., Agazzi A., Baldi A., Balzaretti C., Bersani C., Pirani S., Rebu -cci R., Savoini G., Stella S., Stenico A., Domeneghini C. (2009). Administration of Bacillus coagulans in calves: recovery from fecal samples and evaluation of functional aspects of spores. Vet. Res. Commun., 33: 991-1001.

  • Ripamonti B., Stella S. (2009). Probiotici sporigeni per l’alimentazione animale. Large Animal Review, 15: 7-12.

  • Ripamonti B., Agazzi A., Bersani C., De Dea P., Pecorini C., Pirani S., Rebu -cci R., Savoini G., Stella S., Stenico A., Tirloni E., Domeneghini C. (2011). Screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts. Anaerobe., 17: 97-105.

  • Ripamonti B., Tirloni E., Stella S., Bersani C., Agazzi A., Maroccolo S., Savoi-ni G. (2013). Effects ofaspecies-specific probiotic formulation on multiresistant Escherichia coli isolates from the gut of veal calves. Czech J. Anim. Sci., 5: 201-207.

  • Roodposhti P.M., Dabiri N. (2012). Effects of probiotic and prebiotic on average daily gain, fecal shedding of Escherichia coli, and immune system status in newborn female calves. Asian. Austral. J. Anim., 25, 9: 1255-1261.

  • Salim H.M., Kang H.K., Akter N., Kim D.W., Kim J.H., Kim M.J., Na J.C., Jong H.B., Choi H.C., Suh O.S., Kim W.K. (2013). Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. Poultry Sci., 92: 2084-2090.

  • Savoini G., Agazzi A., Invernizzi G., Cattaneo D., Pinotti L., Baldi A. (2010). Polyunsaturated fatty acids and choline in dairy goats nutrition: Production and health benefits. Small Ruminant Res., 88: 135-144.

  • Signorini M.L., Soto L.P., Zbrun M.V., Sequeira G.J., Rosmini M.R., Frizzo L.S. (2012). Impact of probiotic administration on the health and fecal microbiota of young calves:ameta-analysis of randomized controlled trials of lactic acid bacteria. Res. Vet. Sci., 93: 250-258.

  • Soto L.P., Frizzo L.S., Bertozzi E., Avataneo E., Sequeira G.J., Rosmini M.R. (2010). Molecular microbial analysis of Lactobacillus strains isolated from the gut of calves for potential probiotic use. Vet. Med. Intern., pp. 1-8.

  • Stavric S., Gleeson T.M., Blanchfield B. (1991). Efficacy of undefined and defined bacterial treatment in competitive exclusion of Salmonella from chicks. In: Colonization Control of Human Bacteria Enteropathogens in Poultry. L. C. Blankenship, ed. Academic Press Inc., New York, NY, pp. 323-330.

  • Stella A.V., Paratte R., Valnegri L., Cigalino G., Soncini G., Chevaux E., Dell ’ Orto V., Savoini G. (2007). Effect of administration of live Saccharomyces cerevisiae on milk production, milk composition, blood metabolites and fecal flora in early lactating dairy goats. Small Ruminant Res., 67: 7-13.

  • Sun P., Wang J.Q., Zhang H.T. (2010). Effects of Bacillus subtilis natto on performance and immune function of preweaning calves. J. Dairy Sci., 93: 5851-5855.

  • Sun P., Wang J.Q., Zhang H.T. (2011). Effects of supplementation of Bacillus subtilis natto Na and N1 strains on rumen development in dairy calves. Anim. Feed Sci. Tech., 164: 154-160 Suzuki T., Yamasato K. (1994). Phylogeny of spore-forming lactic acid bacteria based on 16Sr RNAgene sequences. FEMS Microbiol. Lett., 115: 13-18.

  • Timmerman H.M., Koning C.J.M., Mulder L., Rombouts F.M., Beynen A.C. (2004). Monostrain, multistrain and multispecies probiotic -acomparison of functionality and efficacy. Int. J. Food Microbiol., 96: 219-233.

  • Timmerman H.M., Mulder L., Everts H., Van Espen D.C., Vander Wal E., Klaas-sen G., Rouwers S.M.G., Hartemink R., Rombouts F.M., Beynen A.C. (2005). Health and growth of veal calves fed milk replacers with or without probiotics. J. Dairy Sci., 88: 2154-2165.

  • Tsuruta T., Inoue R., Tsukahara T., Matsubara N., Hamasaki M., Ushida K. (2009). Acell preparation of Enterococcus fecalis strain EC-12 stimulates the luminal immunoglobulin Asecretion in juvenile calves. Anim. Sci. J., 80: 206-211.

  • Zaaijer D., Noordhuizen J.P.T.M. (2003). Anovel scoring system for monitoring the relationship between nutritional efficiency and fertility in dairy cows. Irish Vet. J., 56: 145-151.

Annals of Animal Science

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