Effect of Breed and Age on Histopathological Changes in Pig M. Semimembranosus

Dorota Wojtysiak 1 , Katarzyna Połtowicz 2 ,  and Władysław Migdał 3
  • 1 Department of Reproduction and Animal Anatomy, Agricultural University of Krakow, Al. Mickiewicza 24/28, 30-059 Kraków, Poland
  • 2 Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland
  • 3 Department of Animal Products Technology, Agricultural University of Krakow, Balicka 122, 31-149 Kraków, Poland

Effect of Breed and Age on Histopathological Changes in Pig M. Semimembranosus

The aim of the study was to determine the type and extent of histopathological changes in m. semimembranosus of Polish Landrace (PL), Polish Large White (PLW), Duroc, Pietrain, and Puławska pigs at 60, 90, 120, 150, 180 and 210 days of age. Changes in fibre size (atrophy, hypertrophy - giant fibres), changes in fibre shape (angular fibres), degenerative lesions (necrosis with phagocytosis) and connective tissue hypertrophy were evaluated. The presence of giant fibres was the only histopathological change observed in all age groups of PL, PLW, Duroc and Pietrain pigs, with the percentage of pigs with this type of pathology and the frequency of giant, atrophic and angular fibres increasing significantly with age. In Puławska pigs, giant fibres were only found in the oldest pigs aged 210 days. In these animals, giant fibres as well as atrophic fibres (at 180 and 210 days of age) and angular fibres (at 120, 150, 180 and 210 days of age) occurred in the smallest number of animals and were least extensive. Meanwhile, Pietrain pigs were characterized by a greater number of animals, a significantly greater proportion of giant fibres in all analysed age groups, and a greater proportion of atrophic fibres at 180 and 210 days of age compared to the other pig breeds under analysis. For connective tissue hypertrophy and necrosis with phagocytosis, the changes were not extensive. It is concluded that both the advancing age of the animals and selection of the pigs for increased leanness significantly increases the incidence of histopathological changes in muscle tissue, which may directly translate into pork quality.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Bergmann V. (1979). Changes of cardiac and skeletal muscle in pigs following transport stress. Exp. Pathol., 17: 243-248.

  • Bogucka J., Walasik K., Elminowska-Wenda G., Kruczyńska M. (2006). Pathological changes in the structure of semimembranosus muscle from pigs included in the Stamboek and Torhyb breeding programmes. Anim. Sci. Pap. Rep., Suppl., 2, 24: 45-52.

  • Brym P., Kamiński S., Rusc A., Wójcik E. (2002). Allele frequency in ryanodine receptor (RYR1) locus in boars of different breeds. Ann. Anim. Sci., Suppl., 2: 33-35.

  • Dubowitz V., Sewery C. A. (2007). Muscle Biopsy: A Practical Approach. 3rd edition, Saunders Elsevier.

  • Fazarinc G., Čandek-Potokar M., Uršič M., Vrecl M., Pogačnik A. (2002). Giant muscle fibres in pigs with different RYR 1 genotype. Anat. Histol. Embryol., 31: 367-371.

  • Fiedler I., Ender K., Wicke M., Maak S., von Lergenken G., Meyer W. (1999). Structural and functional characteristics of muscle fibres in pigs with different malignant hyperthermia susceptibility (MHS) and different meat quality. Meat Sci., 53: 9-15.

  • Hausmanowa-Petrusewicz I. (1993). Muscle diseases (in Polish). PZWL, Warszawa.

  • Kłosowska D., Kłosowski B., Nowak B. (1995). Histopathological changes in longissimus muscle of Pietrain pigs and its crosses. Proceedings of the 2nd Dummerstorf Muscle Workshop on Muscle Growth and Meat Quality, Rostock, 17-19 May 1995, pp. 84-91.

  • Paciello O., Papparella S. (2009). Histochemical and immunohistochemical approach to comparative neuromuscular diseases. Folia Histochem. Cytobiol., 47: 143-152.

  • Pospiech E., Borzuta K., Łyczyński A., Plókarz W. (1998). Meat defects and their economic importance. Pol. J. Food Nutr. Sci., 7/48, 4: 7-20.

  • Rahelič S., Pauc S. (1980). Fibre types in longissimus dorsi from wild and highly selected pig breeds. Meat Sci., 50, p. 431.

  • Schmitt O., Dumont B. L. (1981). Detection of giant fibres and interpretation of their presence in pig muscle. In Proceedings of the Symposium Agricultural Food Research Society, As, Norway, pp. 53-58.

  • Schubert-Schoppmeyer A., Fiedler I., Nürnberg G., Jonas L., Ender K., Maak S., Rehfeldt C. (2008). Simulation of giant fibre development in biopsy samples from pig longissimus muscle. Meat Sci., 80: 1297-1303.

  • Sobczak M., Lachowicz K., Żochowska-Kujawska J. (2010). The influence of giant fibres on utility for production of massaged products of porcine muscle longissimus dorsi. Meat Sci., 84: 638-644.

  • Solomon M. B., Eastridge J. S. (1987). Occurrence of giant fibres in muscles from wild pigs native to the United Sates. Meat Sci., 20: 75-81.

  • Solomon M. B., West R. L. (1985). Profile of fibre types in muscles from wild pigs native to the United States. Meat Sci. 13: 247-254.

  • Sośnicki A. (1987). Histopathological observation of stress myopathy in M. longissimus in the pig and relationships with meat quality, fattening and slaughter traits. J. Anim. Sci., 65: 584-596.

  • Thuilliez C., Dorso L., Howroyd P., Gould S., Chanut F., Burnett R. (2009). Histopathological lesions following intramuscular administration of saline in laboratory rodents and rabbits. Exp. Toxicol. Pathol., 61: 13-21.

  • Velotto S., Varricchio E., DiPrisco M. R., Stasi T., Crasto A. (2007). Skeletal myocyte types and vascularity in the Black Sicilian pig. Acta Vet. Brno., 76: 163-170.

  • Walasik K., Kłosowska D., Grześkowiak E. (2000). Pathological changes in musculus longissimus lumborum of hybrid pigs with Hampshire genes (in Polish). Rocz. Nauk. Zoot., Suppl., 6: 233-237.

  • Wegner J., Fiedler I., Kłosowska D., Kłosowski B., Ziegan B. (1993). Veranderungen der Muskelfasertypenverteilung im M. longissimus dorsi von Ebern während des Wachstums dargestellt mit verschiedenen histochemischen Methoden. Anat. Histol. Embryol., 22: 355-359.

  • Wimmers K., Fiedler I., Hardge T., Murani E., Schellander K., Ponsuksili S. (2006). QTL for microstructural and biophysical muscle properties and body composition in pigs. BMC Genetics, 7, p. 15.

  • Wojtysiak D., Migdał W. (2006). Effect of body weight on histopathological changes in m. longissimus lumborum of fatteners. Anim. Sci., Suppl., 2: 451-455.


Journal + Issues