Objective: The aim of the study was to determine the content of polyphenols and flavonoids from sixteen selected medicinal plants from the spontaneous Romanian flora and fifteen tinctures obtained with propylene glycol.
Methods: The polyphenols were determined by the Folin-Ciocalteu method while the flavonoids by using a colorimetric method from the 10th edition of the Romanian Pharmacopoeia. The antioxidant activities of the most common nine medicinal plants and fifteen tinctures were determined by DPPH and ABTS methods.
Results: The results highlighted that the phenolic compounds and flavonoids have contributed to their antioxidant activities and the medicinal plants and tinctures included in the study are rich sources of natural antioxidants.
Conclusions: There are a wide variety of extraction methods for the determination of phenolics and flavonoids. The study confirms a correlation between phenolic and flavonoid contents obtained by using the DPPH and ABTS tests.
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1. Piluzza G, Viridis S, Serralutzu F et al.- Uses of plants, animal and mineral substances in Mediterranean ethno-veterinary practices for the care of small ruminants. Journal of Ethnopharmacology. 2015, 168: 87-99
2. Chun OK, Kim DO, Smith N, Schroeder D, Han JT, Lee ChY – Daily consumption of phenolic and total antioxidant capacity from fruit and vegetables in the American diet. Journal of the Science of Food and Agriculture. 2005, 85: 1715-1724
3. Jablonska-Rys E, Zalewska-Korona M, Kalbarczyk J – Antioxidant capacity, ascorbic acid and phenolics content in wild edible fruits. Journal of Fruit and Ornamental Plant Research. 2009, 17, 2: 115-120
4. Guimaraeas R et al – Characterisation of phenolic compunds in wild fruits from Northeastern Portugal. Food Chemistry. 2013, 141: 3721-3730
5. Waterhouse A.: Determination of total phenolics. in Current Protocols in Food Analytical Chemistry. R.E. Wiley, 2001
6. Balogh E.: Antioxidáns kapacitás meghatározása és ennek kialakításában szerepet játszó vegyületek vizsgálata bogyós gyümölcsök esetében. Corvinus University. PhD thesis. Budapest, 2010
7. da Silva L A, Pezzini BR, Soares L – Spectrophotometric determination of the total flavonoid content in Ocimum basilicum L. (Lamiaceae) leaves. Pharmacognosy Magazine. 2015, 11, 41: 96-101
8. Singleton VL, Rossi JA – Colorimetry of total phenolics with phosphomolybdic-phospgotungstic acid reagents. American Journal of Enology and Viticulture. 1965, 16, 3: 144-158
9. He Z, Lan M, Lu D, Zhao H, Yuan H – Antioxidant activity of 50 traditional chinese medicinal materials varies with total phenolics. Chinese Medicine. 2013, 4: 148-152
10. Odeh I, Al-Rimawi F, Abbadi J, Obeyat L, Qabbajeh M, Hroub A – Effect of harvesting date and variety of date palm on antioxidant capacity, phenolic and flavonoid content of date palm (Phoenix dactylifera). Journal of Food and Nutrition Research. 2014, 2, 8: 499-505
11. ***Farmacopeea Română, Xth Edition. București. 1993, 335
12. Brand – Williams W, Cuvelier ME, Berset C – Use of a method to evaluate antioxidant activity. Lebensm-Wiss U.-Technol. 1995, 28: 25-30
13. Emad A, Shalaby & Sanaa M, Shanab M – Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian Journal of Geo-Marine Sciences. 2013, 42, 5: 556-564
14. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M & Rice-Evans C - Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999, 26, 9-10: 1231-1237
15. CH Pearson, DJ Rath: A hydraulic press for extracting fluids from plant tissue sample. Industrial crops and products, 2009, 29: 634-637
16. Tunalier Z, Kosar M, Küpeli E, Calis I, Baser KHC: Antioxidant, anti-inflammatory, anti-nociceptive activities and composition of Lythrum salicaria L. extracts, Journal of Ethopharmacology, 2007, 110: 539-547
17. Lee YI, Thiruvengadam M, Chung I-M, Nagella P: Polyphenol composition and antioxidant activity from the vegetable plant Artemisia absinthium L., Australian Journal of Crop Science, 2013, 7, 12: 1921-1926
18. Tomczyk M, Pleszczyńska M, Wiater A: Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity, Molecules, 2010, 15: 4639-4651