Objective. Amflora potato, event EH92-527-1 was genetically modified to produce only the amylopectin component from starch composition. The presence of the transgenic potato in the food on Romanian market should be verified although the Amflora potato uses for industrial purposes and animal feed. The aim of this study was to detect the presence of the Amflora potato in the food or feed products.
Methods. For this purpose, five samples of potato tubers and four samples of chips were analyzed. DNA isolation was performed with E.Z.N.A. ® MicroElute Genomic DNA kit (Omega Bio-Tek, USA). For identification a potential presence of the recombinant DNA in the food samples it was used GMOIdent RT Event EH92-527-1 Potato kit (Eurofins GeneScan, Germany), that is a real-time PCR kit for qualitative event-specific detection of AmfloraTM EH92-527-1 potato.
Results. Amflora transgenic potato was not detected in any of analyzed samples.
Conclusions. The results of real-time PCR method confirm the absence of Amflora event EH92-527-1 in all potato tubers and chips analyzed samples.
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1. Regulation (EC) No. 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Official Journal of the European Union L268 1–23.
2. Regulation (EC) No. 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC. Official Journal of the European Union L268 24–28.
3. Kuiper HA Davies HV – The Safe Food Risk Analysis Framework suitable for GMOs? A case study. Food Control. 2010; 21:1662-1676.
4. VIB (Vlaams Instituut voor Biotechnologie – Flemish Institute for Biothenology) – Scientific background report Amflora potato. https://www.vib.be/en/news/Documents/VIB%20Background%20report%20Amflora.pdf.
5. Tilocca MG Serratrice G Oggiano MA et al – Monitoring the presence of genetically modified potato EH92-527-1 (BSP-25271-9) in commercial processed food. Ital J Food Safety. 2014; 3(1): 57-59.
6. Abdallah N – Amflora great expectations for GM crops in Europe. GM Crops. 2010; 1:3:109-112.
7. European Commission (EU) 2010/135/EU 2010a. Commission Decision concerning the placing on the market in accordance with Directive 2001/18/EC of the European Parliament and of the Council of a potato product (Solanum tuberosum L. line EH92-527-1) genetically modified for enhanced content of the amylopectin component of starch. In: Official Journal of the European Union L53 04/03/2010.
8. European Union Reference Laboratory for GM Food and Feed EU Database of Reference Methods for GMO Analysis. http://gmo-crl.jrc.ec.europa.eu/gmomethods/entry?db=gmometh&id=qt-eve-st-001&rq=id%3aQT-eve-ST*
9. Center for Environmental Risk Assessment GM Crop Database. http://cera-gmc.org/GmCropDatabaseEvent/EH92-527-1
10. Opinion of the Scientific Panel on Genetically Modified Organisms on a request from the Commission related to the notification (Reference C/SE/96/3501) for the placing on the market of genetically modified potato EH92-527-1 with altered starch composition for cultivation and production of starch under Part C of Directive 2001/18/EC from BASF Plant Science. The EFSA Journal. 2006; 323: 1-20. http://onlinelibrary.wiley.com/doi/10.2903/j.efsa.2006.323/epdf
11. European Commission (EU) 2010/136/EU 2010b. Commission Decision authorizing the placing on the market of feed produced from genetically modified potato EH92-527-1 (BPS-25271-9) and the adventitious and technically unavoidable presence of the potato in food and other feed products under Regulation (EC) No. 1829/2003 of the European Parliament and of the Council. In: Official Journal of the European Union L53 04/03/2010.
14. Event-specific method for the quantification of amylopectin potato event EH92-527-1 using real-time PCR Protocol EH92-527-1 CRLVL09/05VP Validation Report EH92-527-1 potato CRLVL09/05VR Community Reference Laboratory for GM Food and Feed. EH92-527-Validated_method corrected version 1.pdf
15. Fraiture M-A Herman P Taverniers I De Loose M Deforce D Roosens NH – An innovative and integrated approach based on DNA walking to identify unauthorized GMOs. Food Chem. 2014; 147: 60-69.
16. Holst-Jensen A Bertheau Y de Loose M et al – Detecting un-authorized genetically modified organisms (GMOs) and derived materials. otechnology Advances. 2012; 30: 1318-1335.