Characterization of Three-Dimensional Printed Composite Scaffolds Prepared with Different Fabrication Methods

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An optimal method for composites preparation as an input to rapid prototyping fabrication of scaffolds with potential application in osteochondral tissue engineering is still needed. Scaffolds in tissue engineering applications play a role of constructs providing appropriate mechanical support with defined porosity to assist regeneration of tissue. The aim of the presented study was to analyze the influence of composite fabrication methods on scaffolds mechanical properties. The evaluation was performed on polycaprolactone (PCL) with 5 wt% beta-tricalcium phosphate (TCP) scaffolds fabricated using fused deposition modeling (FDM). Three different methods of PCL-TCP composite preparation: solution casting, particles milling, extrusion and injection were used to provide material for scaffold fabrication. The obtained scaffolds were investigated by means of scanning electron microscope, x-ray micro computed tomography, thermal gravimetric analysis and static material testing machine. All of the scaffolds had the same geometry (cylinder, 4×6 mm) and fiber orientation (0/60/120°). There were some differences in the TCP distribution and formation of the ceramic agglomerates in the scaffolds. They depended on fabrication method. The use of composites prepared by solution casting method resulted in scaffolds with the best combination of compressive strength (5.7±0.2 MPa) and porosity (48.5±2.7 %), both within the range of trabecular bone.

[1] R.Tadeusziewicz, P. Augustyniak, Basic Biomedical Engineering 2, AGH , Kraków 2009.

[2] W. Swieszkowski, Ho Saey Tuanb Barnabas, K.J. Kurzydlowski, D.W. Hutmacher, Repair and regeneration of osteochondral defects in the articular joints, Biomolecular Engineering 24, 5, 489-495 (2007).

[3] R. Langer, J. Vacanti, Tissue engineering, Science 260, 920-926(1993).

[4] C.W. Patrick, A.G. Mikos, L.V. Mcintire, Frontiers in Tissue Engineering, New York, Pergamon 1998.

[5] D.W. Hutmacher, Scaffolds in tissue engineering bone and cartilage Biomaterilas 21, 2529-43 (2000).

[6] S.J. Lee, S.H. Oh, J. Liu, S. Soker, A. Atala, J.J. Yoo, The use of thermal treatments to enhance the mechanical properties of electrospun poly(É-caprolactone) scaffolds, Biomaterials 29, 10, 1422-1430 (2008).

[7] P. Hariraksapitak, O. Suwantong, P. Pavasant, P. Supaphol, Effectual drug-releasing porous scaffolds from 1,6-diisocyanatohexane-extended poly(1,4-butylene succinate) for bone tissue regeneration, Polymer 49, 11, 2678-2685 (2008).

[8] C. Hu, C. Tercero, S. Ikeda, M. Nakajima, H. Tajima, Y. Shen, T. Fukuda, A. Fumihito, Biodegradable porous sheetlike scaffolds for soft-tissue engineering using a combined particulate leaching of salt particles and magnetic sugar particles, Journal of Bioscience and Bioengineering 116, 1, 126-131 (2013).

[9] S.A. Poursamara, J. Hatamic, A.N. Lehnerb, C.L. Silvac, F.C. Ferreirac, A.P.M Antunesa, Gelatin porous scaffolds fabricated using a modified gas foaming technique: Characterisation and cytotoxicity assessment, Materials Science and Engineering: C 48, 63-70 (2015).

[10] M.T. Arafat, C.X.F. Lam, A.K. Ekaputra, S.Y. Wong, X. Li, I. Gibson, Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering, Acta Biomaterialia 7, 2, 809-820 (2011).

[11] D.W. Hutmacher, M. Sittinger, M. V. Risbud Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems, Trends Biotechnol 22, 354-362 (2004).

[12] S. J. Hollister Porous scaffold design for tissue engineering, Nat Mater 4, 518-524 (2005).

[13] F. Wang, L. Shor, A. Darling, S. Khalil, W. Sun, S. Guceri et al. Precision extruding deposition and characterization of cellular poly-Îľ-caprolactone tissue scaffolds, Rapid Prototyping J 10, 42-49 (2004).

[14] L. Shor, S. Guceri, X. Wen, M. Gandhi, W. Sun Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblastâscaffold interactions in vitro, Biomaterials 28, 5291-5297 (2007).

[15] M.J. Mondrinos, R. Dembzynski, L. Lu, V. K. C. Byrapogu, D.M. Wootton, P.I. Lelkes, J. Zhou, Porogen-based solid freeform fabrication of polycaprolactoneâcalcium phosphate scaffolds for tissue engineering, Biomaterials 27, 25, 4399-4408 (2006).

[16] C.E. Misch, Z. Qu, M. W. Bidez, Mechanical properties of trabecular bone in the human mandible: implications for dental implant treatment planning and surgical placement, J Oral Maxillofac Surg 57 (6), 700-706. (1999).

Archives of Metallurgy and Materials

The Journal of Institute of Metallurgy and Materials Science and Commitee on Metallurgy of Polish Academy of Sciences

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IMPACT FACTOR 2016: 0.571
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CiteScore 2016: 0.85

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Source Normalized Impact per Paper (SNIP) 2016: 0.740

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