Non-Invasive and Minimum Invasive Concepts in Prevention, Cariology and Endodontics – from Research and Science to Practice


The minimum invasive dental concept is based on practical experience of more than 30 years negative trends from the use of resin materials in distal teeth and early metal-ceramic “aesthetic” crowns and bridges. The scientific dental research includes all aspects of the conservative dentistry treatment which are scientifically and clinically proved with most up to date methods and tests: anthropometric, epidemiological, biochemical, immunohistochemical, electron microscopy, polarized light microscopy, light induced fluorescence, microradiography, microbiology, genetic tests, etc. Experiments in vitro, animal tests, clinical research in the last 34 years are focused in the field of prevention of dental caries, treatment of precavitated precarious lesions with nanotechnologies, preparation of hard dental tissues with most conservative techniques, adhesive restoration methods with amalgam and resins, backup in endodontics in root canals preparation methods and in pins and posts fixations. The conclusions can be focused on basic principles and refocused in the 21st century like: Dental caries is not only predictable and controlled disease but is also a polyetiological disease on a community and population levels and uni- or bi-etiological on an individual level. The minimum invasive dental concept in cariology and endodontics leads to prevention of early extractions, early prosthetic medical procedures and to lifetime dental health. This plays a key role in prevention of metabolic disorders and gastrointestinal diseases.

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  • 8. Boteva E. Clinical studies on the effects of nutrition on the DE- and Re-mineralization of the mineralized dental tissues. Lecture. International Conference of Prophylaxis and Biomaterials. Poznan, 9-10. 06.1997.

  • 9. Boteva E. Proteinmediated fluoridation and enamel demineralization. Lecture. International Conference on Surface and Colloid Science. 6-12.07.1997, Sofia.

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  • 23. Boteva E. Late results of two steps application of dentin bondingagent in the biological treatment of the pulp. Abstr. In English. Problems of Dentistry, 29, 2003, 1, 3-6.

  • 24. Boteva Е, D. Iovchev. The sizes of pulp chambers of molars with severe root curvatures in vitro comparative study. Acta Medica Bulgarica, 39, 2012, 1, 92-96.

  • 25. Boteva Е, D.I ovchev Efficiancy of working length detection and irrigation during preparation of curved root canals. Acta Medica Bulgarica, 39, 2012, 1, 97-103.

  • 26. Boteva Е, D. Iovchev Efficiancy of working length detection and irrigation during preparation of curved root canals. Journal of Bass, 2012, 1, 1-4.

  • 27. Boteva E. In vitro comparison of dentin bonding agent and cementum under composite material in cavities class I and II. Abstr. in English Annual Proceedings of IMAB, 8, 2002, 1, 64-67.

  • 28. Boteva Е. M. Мarinova, D. Karayasheva Аn in vitro study of root caries in front teeth. Acta Medica Bulgarica, 39, 2012, 2. 68-71.

  • 29. Boteva Е, K. Peycheva. Detection of Dental Hypoplasia Ability of Fuorescence methods. Acta Medica Bulgarica, 40, 2013, 1, 61-64.

  • 30. Boteva E, Karayasheva, M. Marinova Frequency of root caries in teeth with sound crowns. Car. Res. 47, 2013, 5, 439, Abstr. 62.

  • 31. Boteva E, K. Peycheva, D. Karayasheva, D. Pashkouleva. A study on fracture resistance of class IV cavities, treated with pins. Acta Medica Bulgarica, 41, 2014, 2, 43-48.

  • 32. Boteva E., K. Peycheva, D. Karayasheva, D. Pashkouleva. А study on fracture resistance of class II cavities, treated with pins. Int J Science Res, 4, 3, 2015, 1722-1724

  • 33. Boteva E., K. Peycheva, D. Karayasheva. Frequency of iatrogenic changes caust from overhang restorations. Acta Medica Bulgarica, 42, 2015, 2, 30-35.

  • 34. Boteva E. Minimum invasive dental concept in prevention and conservative treatment from research and science to practice. IJSR, 5, 2016, 11, 1325-1328.

  • 35. Karayasheva, M. Marinova, Boteva E. Salivary flow rate, buffer capacity, microbial counts,and caries activity of young healthy subjects. Caries Research, 44, 2010, 3, 193, Abstr. 56.

  • 36. Karayasheva D., M. Marinova-Takorova, E. Boteva. A study on salivary clearance in dental caries prediction. Arch. of the Balkan Medical Union, vol.49, 2014, 3, 319-323.

  • 37. Karayasheva D., M. Glushkova, T. Kadiyska, E. Boteva. Association study of the role of Glut 2 receptor in dental caries susceptibility, dietary habits and body mass index-BMI. International Journal of Science and Research, 5, 2016, 3, 83-86.

  • 38. Karayasheva D., M. Glushkova, E. Boteva, V. Mitev, T. Kadiyska Association study for the role of Matrix metalloproteinases 2 and 3 gene polymorphisms in dental caries susceptibility. Arch Oral Biology, 68, 2016, 9-12.

  • 39. Karayasheva D., M. Glushkova, T. Kadiyska, E. Boteva The role of matrix metalloproteinases (MMP2 and MMP3) gene polymorphisms in dental caries susceptibility. Caries Research 50, 2016, 27, Abstr. 59.

  • 40. Marinova-Takorova M, D. Karayasheva, E. Boteva. Evaluation of microleakage at the interface between cavity walls and giomer and silorane based resin-in vitro study. Scripta Scientifica Medicinae Dentalis, 1, 2015, 1, 38-42.

  • 41. Мarinova M., E. Boteva, K. Kalinov The role of unstimulatedsaliva as a risk factor for the development of root caries. Caries Research 49, 2015, p. 315, Abstr. 40.

  • 42. Мarinova M., E. Boteva, K. Kalinov A study on the connection between root caries and periodontal disease. Comptes rendus, 69, 2016, 9, 1239-1244.

  • 43. Peycheva K., E. Boteva. Methods for diagnosing dental fluorosis: Quantitative Laser fluorescence and Light-Indused fluorescence. Acta Medica Bulgarica, 40, 2013, 1, 53-60.

  • 44. Peycheva K., E. Boteva. А Clinical study on dental fluorosis with Light Indused Fluorescence. IJSR, International Journal of Science and Research, 4, 3/2015, 1239-1241.

  • 45. Peycheva K., E. Boteva. А comparison of different methods for fissure caries detection. Acta Medica Bulgarica, 43, 2016, 1, 30-38.

  • 46. Peycheva K., D. Karayasheva, E. Boteva. Diagnostic abilities of light induced fluorescence LIF with SoproLife device. Caries Research 50, 2016, 61, Abstr. 137.

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  • 48. Rugg-Gunn A., E. Boteva. Fluoride and Calcium Retention in the Mouth after Rinsing with F-milk or F-water. Stoma 2000, 3, .223-226.

  • 49. Rugg-Gunn A., E. Boteva. Retention of Fluoride and Calcium in the Mouth after Rinsing with Fluoridated Milk or Fluoridated Water. Caries Research, 31, 1997, 4, 302, Abstr. 65.

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  • 51. Rugg-Gunn A., E. Boteva. Fluoride Retention in Enamel Slabs after Rinsing or Drinking F-milk or F-water, IADR/CED Sept. 1997, Madrid, Journal of Dental Research 1998, 77 May, 5, Abstr 357, 1251, 105.


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