The Potential of New Streptomyces Isolates as Biocontrol Agents Against Fusarium Spp

Mihaela Ursan 1 , Oana Alina Boiu-Sicuia 1 , Cătălina Voaides 1 , Vasilica Stan 1 , Corina Bubueanu 2 , and Călina Petruţa Cornea 1
  • 1 University of Agronomical Sciences and Veterinary Medicine Bucharest, Faculty of Biotechnologies, 59 Mărăști Blvd,, Bucharest, Romania
  • 2 National Institute of Chemical-Pharmaceutical Research and Development,112 Vitan St., District 3,, Bucharest, Romania

Abstract

The excessive use of synthetic pesticides for plant pathogens control could cause possible harmful side-effects to humans and animals, environmental pollution, residual toxicity, affects soil characteristics or induce the development of fungal resistance. Alternative ways for fungal contamination control involve natural products, based on microorganisms, many of them being already available for use. The selection and characterization of new biological agents useful for plant pathogens control are permanent goals for plant protection researches. In the last decade, several studies revealed that Streptomyces species are promising biocontrol agents against a wide range of phytopathogenic fungi, including Fusarium spp., one of the most important wheat pathogens. In our study, 60 strains of Streptomyces spp. were isolated from soil or compost and evaluated for in vitro antifungal abilities by dual confrontation method. At least 30% of the isolates presented inhibitory activity against F. culmorum and F. graminearum. The bacterial strains were also tested for their ability to produce various bioactive compounds, possible involved in fungal inhibition. The capacity of some of the bacterial isolates to produce cellulases, amylases, lipases and volatile metabolites was proved. In addition, a new screening method for chitinases production was developed, based on the use of a combination of Congo red and Lugol solutions, which allowed the detection of chitinases in several Streptomyces spp. strains. Moreover, the reduction of the level of some Fusarium mycotoxins was detected by HPTLC analysis. As a conclusion, antagonistic interactions between Streptomyces isolates and fusaria could involve antibiosis, competition and parasitism and suggested that at least some of the selected isolates could be used in obtaining biological control products.

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