Biochemical Investigations of Different Mushroom Species for Their Biotechnological Potential

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Among bioactive constituents occurring in mushrooms, phenolic compounds focus attention due to their antioxidant activity. Also, a special attention is given to mushrooms secreting extra cellular enzymes such as lignin peroxidases (LiP), manganese peroxidase (MnP) and laccase (Lac), enzymes which can be used in biodegradation processes. The aim of the present study was to investigate ten mushroom species for total phenolic compounds, lignocellulolytic enzymes and for their synthetic dyes decolourisation potential. For this purpose, 70% ethanol extracts of ten dried mushrooms were analysed using spectrophotometric methods. The results revealed that total phenolic compounds in the extracts were the highest in A. campestris, P. ostreatus var. Florida and T. versicolor. Laccase activity showed high values in extracts from P. ostreatus var. Florida, A. campestris, L. edodes, and G. applanatum. Lignin peroxidases (LiP) activity showed high values in extracts from A. campestris, F. velutipes, P. ostreatus var. Florida and T. versicolor, whereas manganese peroxidase (MnP) activity was highest in extracts from P. ostreatus var. Florida, A. campestris and G. applanatum. It was found that some of the fungal extracts showed high activities in decolorizing of synthetic dyes.

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  • Barbosa A.M. Dekker R.F.H. St. Hardy G.E 1996. Veratryl alcohol as an inducer of laccase by an ascomycete Botryosphaeria sp. when screened on the polymeric dye Poly R-478. Letters in Applied Microbiology 23(2): 93-96.

  • Boonsong S. Klaypradit W. Wilgggaipun P. 2016. Antioxidant activities of extracts from five edible mushrooms using different extractants. Agriculture and Natural Resources 50(2): 89-97.

  • Bubueanu C. Grigore A. Șerban E. Popa G. Cornea C.P 2017. HPTLC identification of bioactive compounds and antioxidant activity of Pleurotus ostreatus and Lentinus edodes extracts. Scientific Bulletin. Series F. Biotechnologies Vol. XXI ISSN 2285-1364 343-348.

  • Croft K.C. 1999. Antioxidant effects of plant phenolic compounds. In T. K. Basu N. J. Temple M. L. Garg (Eds.) Antioxidants in Human Health CABI Publishing Wallingford Oxon New York 109-121.

  • Iordache O. Cornea C. P. Popa G. Diguţă C. Dumitrescu I. Vărzaru E. Rodino S. Ionescu I. Matei A. 2016. Evaluation of decolourization abilities of some textile dyes by fungal isolates. Revista Industria textilă ISSN 1222- 5347 vol. 67(3) 181-188.

  • Kagan I.A. Flythe M.D 2014. Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds. J. Vis. Exp. (85) e51411 1-8.

  • Jaouani A. Guillén F. Penninckx M.J. Martínez A.T. Martínez M.J. 2005. Role of Pycnoporus coccineus laccase in the degradation of aromatic compounds in olive oil mill wastewater. Enzyme Microb. Technol. 36(4): 478-486.

  • Jhadav A. Vamsi K.K. Khairnar Y. Boraste A. Gupta N. Trivedi S. Patil P. Gupta G. Gupta M. Mujapara A.K. Joshi B. Mishra d. 2009. Optimization of production and partial purification of laccase by Phanerochaete chrysosporium using submerged fermentation. International Journal of Microbiology Research 1(2): 9-12.

  • Kalra K. Chauhan R. Shavez M. Sachdeva S. 2013. Isolation of laccase producing Trichoderma spp. and effect of pH and temperature on its activity. Int. J. Chem. Environ. Technol. 5(5): 2229- 2235.

  • Khatua S. Paul S. Acharya K. 2013. Mushroom as the potential source of new generation of antioxidant: a review. Res J Pharm Technol 6(5): 496-505.

  • Lim S-H. Lee Y-H. Kang H-W. 2013. Efficient recovery of lignocellulolytic enzymes of spend mushroom compost from oyster mushrooms Pleurotus spp. and potential use in dye decolorization. Mycobiology 41(4): 214-220.

  • Mabrouk A.M. Kheiralla Z.H. Hamed E.R. Youssry A.A. Abd El Aty A.A. 2010. Screening of some marinederived fungal isolates for lignin degrading enzymes (LDEs) production. Agriculture and Biology Journal of North America 1(4): 591-599.

  • Maciel M. J. M. Castro e Silva A. Ribeiro H. C. T 2010. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: a review. Electronic Journal of Biotechnology 13(6): 14-15.

  • Michniewicz A. Ledakowicz S. Ullrich R. Hofrichter M. 2008. Kinetics of the enzymatic decolorization of textile dyes by laccase from Cerrena unicolor Dyes and Pigments 77(2): 295-302.

  • Nagai M. Sato T. Watanabe H. Saito K. Kawata M. Enei H. 2002. Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes and decolorization of chemically different dyes. Appl. Environ. Microbiol. 60(3): 327-335.

  • Nigam P. S. 2013. Microbial enzymes with special characteristics for biotechnological applications. Biomolecules 3(3):597-611.

  • Palonen H. Viikari L. 2004. Role of oxidative enzymatic treatments on enzymatic hydrolysis of softwood. Biotechnol. Bioeng. 86(5): 550-557.

  • Patel Y. Naraian R. Singh V. K. 2012. Medicinal properties of Pleurotus species (oyster mushroom): a review. World Journal of Fungal and Plant Biology 3(1): 1-12.

  • Popa G. Nicolcioiu M. B. Cornea C.P. 2013 In vitro evaluation of crude oil degradation potential of some Pleurotus ostreatus isolates. Scientific Bulletin. Series F. Biotechnologies Vol. XVII ISSN 2285-1364 143-146.

  • Popa G. Cornea C.P. Luta G. Gherghina E. Israel Roming F. Bubueanu C. Toma R. 2016. Antioxidant and antimicrobial properties of Laetiporus sulphureus (Bull.) Murrill. AgroLife Scientific Journal - Volume 5 (1): 168 -173.

  • Singleton V. L. Orthofer R. Lamuela-Raventos R. M. Lester P. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth. Enzymol. 299: 152-178.

  • Takamiya M. Magan N. Warner P.J. 2008. Impact assessment of bisphenol A on lignin-modifying enzymes by basidiomycete Trametes versicolor. Journal of Hazardous Materials 154(1-3): 33-37.

  • Teixeira R.S. Pereira P.M. Ferreira-Leitao V. 2010. Extraction and Application of Laccases from Shimeji Mushrooms (Pleurotus ostreatus) Residues in Decolourisation of Reactive Dyes and a Comparative Study Using Commercial Laccase from Aspergillus oryzae. Enzyme Research 2010: 1-8.

  • Wani B. A. Bodha R. H. and Wani A. H. 2010. Nutritional and medicinal importance of mushrooms. Journal of Medicinal Plants Research 4(24): 2598-2604.

  • Wasser S.P. 2014. Medicinal mushroom science: Current perspectives advances evidences and challenges. Biomed. Journal 37(6):345-56.

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