The Presence of Iron and Iucc Virulence-Associated Genes in Romanian Apec Isolates

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Abstract

Colibacillosis in poultry is relating with the colonisation with so called Avian Pathogen E. coli (APEC) strains. It is already known that usually in the APEC isolates are present at least 13 virulence-associated genes. We selected 12 non-repetitive E. coli isolates from different Romanian poultry outbreaks. Isolates have been evaluated for the presence of the virulence-associated genes, iroN and iucC. The DNA extraction was made using QIAamp cador Pathogen Mini Kit (Qiagen). The amplification protocol was: a cycle of denaturation at 94°C for 5 min followed by 35 cycles of 94°C for 30 s, 63°C for 45 s and 72°C for 105 s, and a cycle of 72°C for 7 min. Both virulence-associated genes were identified in 83.33% (11/12) isolates. In two APEC isolates, only one gene was identified, iroN or iucC, respectively. According to these preliminary results it could be assumed that iroN and iucC genes are independently expressing their virulence.

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  • Awwad E. K. Adwan M. Farraj T. Essawi I. Rumi A. Manasra S. Baraitareanu M. R. Gurau and D. Danes. 2015. Cell Envelope Virulence Genes among Field Strains of Brucella melitensis Isolated in West Bank Part of Palestine. Agriculture and Agricultural Science Procedia. 6: 281-286.

  • Akram N. H. Mojtaba A. Azam A. Masoud and H. Najmeh 2017. ʺDistribution of pathogenicity island markers and virulence factors in new phylogenetic groups of uropathogenic Escherichia coli isolatesʺ. Folia Microbiologica 63(3):335-343 accessed february 2018 doi:

    • Crossref
    • Export Citation
  • Barnes H.J. J.R. Glisson A.M. Fadly L.R. McDougald and D. Swayne 2003. Colibacillosis. In Saif Y.M. Barnes H.J. Glisson J. R. Fadly A. M. McDougald L.R. Swayne D. E. (Eds.) Diseases of Poultry 11th edn. Ames Iowa State University Press 631-652.

  • Baumler A.J. T.L. Norris T. Lasco W. Voigt R. Reissbrodt W. Rabsch and F. Heffron 1998. IroN a novel outer membrane siderophore receptor characteristic of Salmonella enterica. Journal of Bacteriology. 180: 1446-1453.

  • Chakraborty A. V. Saralaya P. Adhikari S. Shenoy S. Baliga and A. Hegde 2015. Characterization of Escherichia coli phylogenetic groups associated with extraintestinal infections in South Indian population Ann Med Health Sci Res. 5(4): 241-246.

  • Delicato E.R. B.G.de Brito L.C.J. Gaziri and M.C. Vidotto 2003. Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis. Veterinary Microbiology. 94: 97-103.

  • Dho-Moulin M. and J.M. Fairbrother 1999. Avian pathogenic Escherichia coli (APEC).Veterinary Research. 30: 299-316.

  • Dou X. J. Gong X. Han M. Xu H. Shen D. Zhang L. Zhuang J. Liu and J. Zou 2016. Characterization of avian pathogenic Escherichia coli isolated in eastern China Gene Gene. 576 : 244-248.

  • Dozois C.M. F. Daigle and R. Curtiss 2003. Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain. Proceedings of the National Academy of Sciences U. S. A..100: 247-252.

  • Ewers C. Li G. H. Wilking S. Kiessling K. Alt E.M. Anta`o C. Laturnus I. Diehl S. Glodde T. Homeier U. Bo ¨hnke H. Steinru ¨ck H.C. Philipp L.H. Wieler 2007. Avian pathogenic uropathogenic and newborn meningitis-causing Escherichia coli: How closely related are they? International Journal of Medical Microbiology.297: 163-176.

  • Johnson T.J. Y. Wannemuehler C. Doetkott S.J. Johnson S.C. Rosenberger and L.K. Nolan. 2008. Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool. Journal of Clinical Microbiology 46: 3987-3996.

  • Li G. C. Ewers C. Laturnus I. Diehl K. Alt J. Dai E.M. Anta ˜o K. Schnetz and L.H. Wieler 2008. Characterization of ayjjQ mutant of avian pathogenic Escherichia coli (APEC). Microbiology. 154: 1082-1093.

  • Lixiang Z. G. Song H. Haixia X. Xiaojing Z. Xiaoping Y. Weixia G. Qingqing and L. Xiufan 2009. Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model. Microbiology. 155: 1634-1644.

  • QIAamp cador Pathogen Mini Kit manufacturer’s instructions https://www.qiagen.com/us/shop/sample-technologies/dna/genomic-dna/qiaamp-cadorpathogen-mini-kit/#productdetails

  • Rouquet G. G. Porcheron C. Barra M. Re ´pe ´rant N.K. Chanteloup C. Schouler and P. Gilot 2009. A metabolic operon in extraintestinal pathogenic Escherichia coli promotes fitness under stressful conditions and invasion of eukaryotic cells. Journal of Bacteriology. 191: 4427-4440.

  • Skyberg J.A. S.M. Horne C.W. Giddings R.E. Wooley P.S. Gibbs and L.K. Nolan. 2003. Characterizing avian Escherichia coli isolates with multiplex polymerase chain reaction. Avian Disease. 47 : 1441-1447.

  • Tudorache M. I. Custura I. Van A. Marmandiu P. Anton. 2016. Influence of rearing technology on body weight of young broiler breeders. AgroLife Scientific Journal. 5: 160-165

  • Vandekerchove D. F. Vandemaele C. Adriaensen M. Zaleska J.P. Hernalsteens L. De Baets P. Butaye F. Van Immerseel P. Wattiau H. Laevens J. Mast B. Goddeeris and F. Pasmans 2005. Virulenceassociated traits in avian Escherichia coli: comparison between isolates from colibacillosisaffected and clinically healthy layer flocks. Veterinary Microbiology.108: 75- 87.

  • W. A. van der Westhuizen and R. R. Bragg. 2012. Multiplex polymerase chain reaction for screening avian pathogenic Escherichia coli for virulence genes. Avian Pathology. 41: 33-40.

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