Quantifying Bisphenol from Food Packaging and Assessment of its Cytotoxic Potential

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Bisphenol A (BPA) is a compound that is released from PET packaging during storage bottled water and can have a harmful effect on human health. This study aimed to investigate the cytotoxic potential of BPA at the Caco-2 cell line. Bottled water samples coming from two companies were analyzed starting from 1 month to 12 months of storage Quantification of BPA in water stored in PET containers was carried out by a spectrometric method. Calibration was performed for BPA concentrations ranging between 0.01 and 0.09 mg/L. Caco-2 cell cultures were treated for 24 hours with different BPA concentrations (from 0.1 mM to 1 mM). Viability and cell cytotoxicity analysis was performed by MTT assay. BPA concentration in one water sample ranged from 0.09 to 0.92 mg/L while for second water sample BPA concentration ranged from 0.03 to 0.73 mg/L. After 10 months from packing, BPA concentration began to exceed the 0.6 mg/L limit stated by Regulation (EU) no. 10/2011. Cell viability decreased in a dose dependent manner and reached the minimum of 3.8% for the 1 mM BPA concentration.

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  • [1] Huang Y. Wong C. Zheng J. Bouwman H. Barra R. Wahlstrom B. Neretin L. Hong M. (2012) Bisphenol A (BPA) in China: A review of sources environmental levels and potential human impacts. Environ. Int. 42 91-99.

  • [2] Kang J. H. Kondo F. Katayama Y. (2006) Human exposure to bisphenol A. Toxicology 226 79-89.

  • [3] Michalowicz J. (2014) Bisphenol A - Sources toxicity and biotransformation. Environmental Toxicology and Pharmacology 37 738-758.

  • [4] Rochester J. (2013) Bisphenol A and human health: A review of the literature Reproductive Toxicology 42 132-155.

  • [5] Hunt P. A. Koehler K. E. Susiarjo M. Hodges C.A. Ilagan A. Voigt R.C. et al. (2003) Bisphenol A exposure causes meiotic aneuploidy in the female mouse. Curr Biol. 13(7) 546-553.

  • [6] Mustieles V. (2015) Bisphenol A: Human exposure and neurobehavior NeuroToxicology 49 174-184.

  • [7] Calafat A. M. Ye X. Silva M. J. Kuklenyik Z. Needham L. L. (2006) Human exposure assessment to environmental chemicals using biomonitoring. Int. J. Androl. 29(1) 166-171.

  • [8] Hiroshi I Kazue M Masamichi D Takayuki M Fumio Y (2003) Bisphenol A-induced apoptosis of cultured rat Sertoli cells Reproductive Toxicology 17 (4). 457-464.

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