Modern analytical techniques in the assessment of the authenticity of Serbian honey / Moderne analitičke tehnike u procjeni izvornosti meda iz Srbije

Food authenticity in a broader sense means fulfilling chemical and physical criteria prescribed by the proposed legislation. In the case of honey authenticity, two aspects are of major concern: the manufacturing process and the labelling of final products in terms of their geographical and botanical origin. A reliable assessment of honey authenticity has been a longterm preoccupation of chemists-analysts and it usually involves the use of several criteria and chemical markers, as well as a combination of analytical and statistical (chemometric) methods. This paper provides an overview of different criteria and modern methods for the assessment of honey authenticity in the case of a statistically significant number of authentic honey samples of several botanical types from various regions of Serbia.

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  • 1. Merriam Webster An Encyclopaedia Britannica Company [displayed 1 December 2015]. Available at www.merriamwebster.com

  • 2. Carcea M. Food authenticity - legal aspects and analytical solutions. In: 7th Food Engineering Congress “Chamber of Food Engineers”; 24-26 November 2011; Ankara, Turkey.

  • 3. Esslinger S, Riedl J, Fauhl-Hassek C. Potential and limitations of non-targeted fingerprinting for authentication of food in official control. Food Res Int 2014;60:189-204. doi: 10.1016/j.foodres.2013.10.015

  • 4. Reid LM, O’Donnell CP, Downey G. Recent technological advances for the determination of food authenticity. Trends Food Sci Technol 2006;17:344-53. doi: 10.1016/j. tifs.2006.01.006

  • 5. Primrose S, Woolfe M, Rollinson S. Food forensics: methods for determining the authenticity of foodstuff. Trends Food Sci Technol 2010;21:582-90. doi: 10.1016/j.tifs.2010.09.006

  • 6. Oliveri P, Downey G. Multivariate class modeling for the verification of food-authenticity claims. Trends Anal Chem 2012;35:74-86. doi: 10.1016/j.trac.2012.02.005

  • 7. Tzouros NE, Arvanitoyannis IS. Agricultural produces: synopsis of employed quality control methods for the authentication of foods and for the classification of foods according to their variety of geographical origin. Crit Rev Food Sci Nutr 2001;41:287-319. doi: 10.1080/20014 091091823

  • 8. Vlachos A, Arvanitoyannis IS. A review of rice authentication/ adulteration methods and results. Crit Rev Food Sci Nutr 2008;48:553-98. doi: 10.1080/10408390701558175

  • 9. Arvanitoyannis IS, Vlachos A. Maize authentication: quality control methods and multivariate analysis (chemometrics).Crit Rev Food Sci Nutr 2009;49:501-37. doi: 10.1080/10408390802068140

  • 10. Da-Wen Sun, editor. Modern Techniques for Food Authentication. Burlington (MA): Academic Press; 2008.

  • 11. Cubero-Leon E, Peñalver R, Maquet A. Review on metabolomics for food authentication. Food Res Int 2014;60:95-107. doi: 10.1016/j.foodres.2013.11.041

  • 12. European Economic Community. EEC Council directive of 20 December 2001 relating to honey. Off J Eur Commun 2002;110:47-50.

  • 13. Codex Alimentarius Commission. Revised Codex Standard for Honey, CODEX STAN 12-1981, Rev. 1 (1987), Rev. 2 (2001).

  • 14. Belitz H-D, Grosch W, Schieberle P. Food Chemistry. 4th ed.Berlin Heidelberg: Springer-Verlag; 2009.

  • 15. Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM.Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chem 2009; 114:1438-43.doi:10.1016/j.foodchem.2008.11.028

  • 16. Kaškonienė V, Veskutonis PR. Floral markers in honey of various botanical and geographic origins: a review. Compr Rev Food Sci Food Saf 2010; 9:620-34.doi:10.1111/j.1541-4337.2010.00130.x

  • 17. Alvarez-Suarez JM, Tulipani S, Romandini S, Bertoli E, Battino M. Contribution of honey in nutrition and human health: a review. Mediterr J Nutr Metab 2010;3:15-23. doi: 10.1007/s12349-009-0051-6

  • 18. Giorgi A, Madeo M, Baumgartner J, Lozzia GC. The relationships between phenolic content, pollen diversity, physicochemical information and radical scavenging activity in honey. Molecules 2011;16:336-47. doi: 10.3390/ molecules16010336

  • 19. Castro-Várquez LM, Díaz-Maroto MC, De Tores C, Pérez- Coello MS. Effects of geographical origins on the chemical and sensory characteristics of chestnut honeys. Food Res Int 2010;43:2335-40. doi: 10.1016/j.foodres.2010.07.007

  • 20. Economou G, Panagopoulos G, Tarantilis P, Kalivas D, Kotoulas V, Travlos IS, Polysio M, Karamanos A. Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus L. and Satureja thymbra L. populations from the Greek island Ikaria. Ind Crops Prod 2011;33:236-41. doi: 10.1016/j. indcrop.2010.10.021

  • 21. Zábrodská B, Vorlová L. Adulteration of honey and available methods for detection - a review. Acta Vet Brno 2014;83:S85-S102. doi: 10.2754/avb201483S10S85

  • 22. Bogdanov S, Martin P. Honey authenticity: a review. Mitt Geb Lebensmittelunters Hyg 2002;93:232-54.

  • 23. Arvanitoyannis IS, Chalhoub C, Gotsiou P, Lydakis- Simantiris N, Kefalas P. Novel quality control methods in conjunction with chemometrics (multivariate analysis) for detecting honey authenticity. Crit Rev Food Sci Nutr 2005;45:193-203. doi: 10.1080/10408690590956369

  • 24. Louveaux J, Maurizio A, Vorwohl G. Methods of melissopalynology. Bee World 1978;59:139-57. doi: 10.1080/0005772X.1978.11097714

  • 25. Von der Ohe W, Persano Odo L, Piana ML, Morlot M, Martin P. Harmonized methods of melissopalynology. Apidologie 2004;35(Suppl 1):S18-S25. doi: 10.1051/apido:2004050

  • 26. Anklam E. A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chem 1998;63:549-62. doi: 10.1016/S0308-8146(98)00057-0

  • 27. Piana M, Persano Oddo L, Bentabol A, Bruneau E, Bogdanov S, Guyot Declerck C. Sensory analysis applied to honey: state of the art. Apidologie 2004;35(Suppl 1):S26-S37. doi: 10.1051/apido:2004048

  • 28. Sabatini A, Bortolotti L, Marcazzan GL. [Conoscere il miele, in Italian]. 5th ed. Bologna: CRA-API, Istituto Nazionale di Apicoltura; 2009.

  • 29. Anupama D, Bhat KK, Sapna VK. Sensory and physicochemical properties of commercial samples of honey. Food Res Int 2003;36:183-91. doi: 10.1016/S0963-9969(02)00135-7

  • 30. Lušić D, Koprivnjak O, Ćurić D, Sabatini A, Conte L. Volatile profile of Croatian lime tree (Tilia sp.), fir honeydew (Abies alba) and sage (Salvia officinalis) honey. Food Technol Biotechnol 2007;45:156-65.

  • 31. Persano Oddo L, Piazza M, Sabatini A, Accorti M.Characterization of unifloral honeys. Apidologie 1995;26:453-65. doi: 10.1051/apido:19950602

  • 32. Mandić M, Primorac L, Kenjerić D, Bubalo D, Perl A, Flanjak I. Characterization of oak mistletoe and common thistle honeys by physicochemical, sensory and melissopalinology parameters. Deut Lebensm-Rundsch 2006;102:245-49.

  • 33. Persano Oddo L, Piana L, Bogdanov S, Bentabol A, Gotsiou P, Kerkvliet J, Martin P, Morlot M, Ortiz Valbuena A, Ruoff K, von der Ohe K. Botanical species giving unifloral honey in Europe. Apidologie 2004;35(Suppl 1):S82-S93. doi: 10.1051/apido:2004045

  • 34. Persano Oddo L, Piro R, Bruneau E, Guyot-Declerck C, Ivanov T, Piškulova J, Flamini C, Lheritier J, Morlot M, von der Ohe W, von der Ohe K, Gotsiou P, Karabournioti S, Kefalas P, Passaloglou-Katrali M, Thrasyvoulou A, Tsigouri A, Marcazzan G, Piazza M, Sabatini AG, Kerkvliet J, Godinho J, Bentabol A, Ortiz Valbuena A, Bogdanov S, Ruoff K. Main European unifloral honeys: descriptive sheets.Apidologie 2004;35(Suppl 1):S38-S81. doi: 10.1051/ apido:2004049.

  • 35. Piasenzotto L, Gracco L, Conte L, Bogdanov S. Application of solid phase microextraction to evaluate traces of thymol in honey. Apidologie 2002;33:545-52. doi: 10.1051/ apido:2002038

  • 36. Persano Oddo L, Sabatini A, Accorti M, Colombo R, Marcazzan G, Piana L, Piazza M, Pulcini P. [I mieli uniflorali Italiani. Nuove schede di caratterizzazione, in Italian]. Roma: Ministero delle Politiche Agricole e Forestali, Istituto Sperimentale per la Zoologia Agraria; 2000.

  • 37. Bogdanov S, Ruoff K, Persano Oddo L. Physico-chemical methods for the characterization of unifloral honeys: a review. Apidologie 2004;35(Suppl 1):S4-S17. doi: 10.1051/apido:2004047

  • 38. Šarić G, Matković D, Hruškar M, Vahčić N. Characterisation and classification of Croatian honey by physicochemical parameters. Food Technol Biotechnol 2008;46:355-67.

  • 39. Ouchemoukh S, Schweitzer P, Bachir-Bey M, Djoudad-Kadji H, Louaileche H. HPLC sugar profiles of Algerian honeys.Food Chem 2010 ; 121 : 561 - 8.doi: 10.1016/j.foodchem.2009.12.047

  • 40. Chudzinska M, Baralkiewicz D. Estimation of honey authenticity by multielements characteristics using inductively coupled plasma-mass spectrometry (ICP-MS) combined with chemometrics. Food Chem Toxicol 2010;48:284-90. doi: 10.1016/j.fct.2009.10.011

  • 41. Bernal JL, Nozal MJ, Toribio L, Diego JC, Ruiz A. A comparative study of several HPLC methods for determining free amino acid profiles in honey. J Sep Sci 2005;28:1039-47. doi: 10.1002/jssc.200500008.

  • 42. Cajka T, Hajslova J, Pudil F, Riddellova K. Traceability of honey origin based on volatiles pattern processing by artificial neural networks. J Chromatogr A 2009;1216:1458-62. doi: 10.1016/j.chroma.2008.12.066

  • 43. Jerković I, Tuberoso CIG, Marijanović Z, Jelić M, Kasuma A. Headspace, volatile and semi-volatile patterns of Paliurus spina-christi unifloral honey as markers of botanical origin. Food Chem 2009;112:239-45. doi: 10.1016/j. foodchem.2008.05.080

  • 44. Kenjerić D, Mandić ML, Primorac Lj, Bubalo D, Perl A. Flavonoid profile of Robinia honeys produced in Croatia. Food Chem 2007;102:683-90. doi: 10.1016/j.foodchem.2006.05.055

  • 45. Kropf U, Korošec M, Bertoncelj J, Ogrinc N, Nečemer M, Kump P, Golob T. Determination of the geographical origin of Slovenian black locust, lime and chestnut honey. Food Chem 2010;121:839-46. doi: 10.1016/j.foodchem.2009.12.094

  • 46. Elflein L, Raezke KP. Improved detection of honey adulteration by measuring differences between 13C/12C stable carbon isotope ratios of protein and sugar compounds with a combination of elemental analyzer - isotope ratio mass spectrometry and liquid chromatography - isotope ratio mass spectrometry (δ13C-EA/LC-IRMS) Apidologie 2008;39:574-87. doi: 10.1051/apido:2008042

  • 47. Bertoncelj J, Golob T, Kropf U, Korošec M. Characterisation of Slovenian honeys on the basis of sensory and physicochemical analysis with a chemometric approach. Int J Food Sci Technol 2011 ; 4 6 : 1 6 6 1 - 7 1 . d o i : 10.1111/j.1365-2621.2011.02664.x

  • 48. Varmuza K, Filzmaser P. Introduction to Multivariate Statistical Analysis in Chemometrics. Boca Raton: CRC Press, Taylor & Francis Group; 2008.

  • 49. Stevanović V, Tan K, Iatrou G. Distribution of the endemic Balkan flora on serpentine I. - obligate serpentine endemics Plant Syst Evol 2003;242:149-70. doi: 10.1007/s00606-003-0044-8

  • 50. Mačukanović-Jocić M. Biologija medonosnog bilja sa atlasom apiflore Srbije [The biology of melliferous plants with an atlas of Serbian apiflora, in Serbian]. Beograd: Poljoprivredni fakultet Univerziteta; 2008.

  • 51. The Statistical Office of the Republic of Serbia [displayed 3December 2015]. Available at http://www.webrzs.stat.gov.rs

  • 52. The Serbian Federation of Beekeeping Organizations [displayed 3 December 2015]. Available at http://www.spos.info

  • 53. Agriculture in the European Union, Statistical and economic information, 2011 [displayed 3 December 2015]. Available at http://ec.europa.eu/agriculture/statistics/agricultural/2011/pdf/full-report_en.pdf

  • 54. Lazarević KB, Andrić F, Trifković J, Tešić Ž, Milojković- Opsenica D. Characterisation of Serbian unifloral honeys according to their physicochemical parameters. Food Chem 2012;132:2060-4. doi: 10.1016/j.foodchem.2011.12.048

  • 55. Bogdanov S, Martin P, Lüllmann C. Harmonized methods of the European Honey Commission. Apidologie 1997;28(extra issue):1-59.

  • 56. Thrasyvoulou A, Manikis J. Some physicochemical and microscopic characteristic of Greek unifloral honeys.Apidologie 1995;26:441-52. doi: 10.1051/apido:19950601

  • 57. Devillers J, Morlot M, Pham-Delègue MH, Doré JC.Classification of monofloral honeys based on their quality control data. Food Chem 2004;86:305-12. doi: 10.1016/j. foodchem.2003.09.029

  • 58. Tucak Z, Periškić M, Škrivanko M, Konjarević A. The influence of the botanic origin of honey plants on the quality of honey. Agriculture 2007;13:234-6.

  • 59. Conti ME, Stripeikis J, Campanella L, Cucina D, Tudino M B. Characterization of Italian honeys (Marche Region) on the basis of their mineral content and some typical quality parameters. Chem Cent J 2007;1:14. doi: 10.1186/1752-153X-1-14

  • 60. Rebane R, Herodes K. Evaluation of the botanical origin of Estonian uni and polyfloral honeys by amino acid content.J Agric Food Chem 2008;56:10716-20. doi: 10.1021/ jf8018968

  • 61. Cometto PM, Faye PF, Naranjo RDDP, Rubio MA, Aldao MA J. Comparison of free amino acids profile in honey from three Argentinian regions. J Agric Food Chem 2003;51:5079-87. doi: 10.1021/jf021081g

  • 62. Iglesias MT, de Lorenzo C, del Carmen Polo M, MartínȦlvarez PJ, Pueyo E. Usefulness of amino acid composition to discriminate between honeydew and floral honeys.Application to honeys from a small geographic area. J Agric Food Chem 2004;52:84-9. doi: 10.1021/jf030454q

  • 63. Senyuva HZ, Gilbert J, Silici S, Charlton A, Dal C, Gürel N, Cimen D. Profiling Turkish honeys to determine authenticity using physical and chemical characteristics. J Agric Food Chem 2009;57:3911-9. doi: 10.1021/jf900039s

  • 64. Cotte JF, Casabianca H, Giroud B, Albert M, Lheritier J, Grenier-Loustalot MF. Characterization of honey amino acid profiles using high-pressure liquid chromatography to control authenticity. Anal Bioanal Chem 2004;378:1342-50. Doi: 10.1007/s00216-003-2430-z

  • 65. Hermosín I, Chicón RM, Cabezudo MD. Free amino acid composition and botanical origin of honey. Food Chem 2003;83:263-8. doi: 10.1016/S0308-8146(03)00089-X

  • 66. Perez RA, Iglesias M, Pueyo E, Gonzalez M, de Lorenzo C.Amino acid composition and antioxidant capacity of Spanish honeys. J Agric Food Chem 2007;55:360-5. doi: 10.1021/ jf062055b

  • 67. Kečkeš J, Trifković J, Andrić F, Jovetić M, Tešić Ž, Milojković-Opsenica D. Amino acids profile of Serbian unifloral honeys. J Sci Food Agric 2013;93:3368-76. doi: 10.1002/jsfa.6187

  • 68. Kečkeš S.,Gašić U, Ćirković Veličković T, Natić M, Milojković-Opsenica D, Tešić Ž. The determination of phenolic profiles of Serbian unifloral honeys using ultrahigh- performance liquid chromatography/high resolution accurate mass spectrometry. Food Chem 2013;138:32-40. doi: 10.1016/j.foodchem.2012.10.025

  • 69. Viuda-Martos M, Ruiz-Navajas Y, Fernández-López J, Pérez- Alvarez JA. Functional properties of honey, propolis, and royal jelly. J Food Sci 2008;73:117-24. doi: 10.1111/j.1750-3841.2008.00966.x

  • 70. Truchado P, Ferreres F, Bortolotti L, Sabatini AG, Tomas- Barberan FA. Nectar flavonol rhamnosides are floral markers of acacia (Robinia pseudoacacia) honey. J Agric Food Chem 2008;56:8815-24. doi: 10.1021/jf801625t

  • 71. Truchado P, Tourn E, Gallez LM, Moreno DA, Ferreres F, Tomás-Barberán FA. Identification of botanical biomarkers in Argentinean Diplotaxis honeys: flavonoids and glucosinolates. J Agric Food Chem 2010;58:12678-85. doi: 10.1021/jf103589c

  • 72. de Rijke E, Out P, Niessen WMA, Ariese F, Gooijer C, Brinkman UATh. Analytical separation and detection methods for flavonoids. J Chromatogr A 2006;1112:31-63. doi: 10.1016/j.chroma.2006.01.019

  • 73. Makarov A, Scigelova M. Coupling liquid chromatography to Orbitrap mass spectrometry. J Chromatogr A 2010;1217:3938-45. doi: 10.1016/j.chroma.2010.02.022

  • 74. Perez DP, Sanchez MT, Cano G, Garrido A. Authentication of green asparagus varieties by near-infrared reflectance spectroscopy. J Food Sci 2001;66:323-7. doi: 10.1111/j.1365-2621.2001.tb11340.x

  • 75. Cocchi M, Durante C, Foca G, Marchetti A, Tassi L, Ulrici A. Durum wheat adulteration detection by NIR spectroscopy multivariate calibration. Talanta 2006;68:1505-11. doi: 10.1016/j.talanta.2005.08.005

  • 76. Davies AM, Radovic B, Fearn T, Anklam E. A preliminary study on the characterisation of honey by near infrared spectroscopy. J Near Infrared Spectrosc 2002;10:121-35. doi: 10.1255/jnirs.329

  • 77. Ruoff K, Iglesias MT, Luginbühl W, Bosset JO, Bogdanov S, Amado R. Quantitative analysis of physical and chemical measurands in honey by mid-infrared spectrometry. Eur Food Res Technol 2006;223:22-9. doi: 10.1007/s00217-005-0085-z

  • 78. Gallardo-Velazques T, Osorio-Revilla G, Zuniga-de Loa M, Rivera-Espinoza Y. Application of FTIR-HATR spectroscopy and multivariate analysis to the quantification of adulterants in Mexican honeys. Food Res Int 2009;42:313-8. doi: 10.1016/j.foodres.2008.11.010

  • 79. Lenhardt L, Zeković I, Dramićanin T, Tešić Ž, Milojković- Opsenica D, Dramićanin MD. Authentication of the botanical origin of unifloral honey by infrared spectroscopy coupled with support vector machine algorithm. Phys Scr 2014;T162:014042. doi: 10.1088/0031-8949/2014/ T162/014042

  • 80. Lazarević KB, Trifković JĐ, Andrić FLj, Tešić ŽLj, Anđelković IB, Radović DI, Nedić NM, Milojković- Opsenica DM. Quality parameters and pattern recognition methods as a tool in tracing regional origin of multifloral honey. J Serb Chem Soc 2013;78:1875-92. doi: 10.2298/ JSC130701099L

  • 81. Gašić U, Kečkeš S, Dabić D, Trifković J, Milojković- Opsenica D, Natić M, Tešić Ž. Phenolic profile and antioxidant activity of Serbian polyfloral honeys. Food Chem 2014;145:599-607. doi:10.1016/j.foodchem.2013.08.088

  • 82. Tomas-Barberan FA, Martos I, Ferreres F, Radovic BS, Anklam E. HPLC flavonoid profiles as markers for the botanical origin of European unifloral honeys. J Sci Food Agric 2001;81:485-96. doi: 10.1002/jsfa.836

  • 83. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.

  • 84. Li H, Wang X, Li P, Li Y, Wang H. Comparative study of antioxidant activity of grape (Vitis vinifera) seed powder assessed by different methods. J Food Drug Anal 2008;16:67-73.

  • 85. Bertoncelj J, Doberšek U, Jamnik M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem 2007;105:822-8. doi: 10.1016/j.foodchem.2007.01.060

  • 86. Dobre B, Gadei G, Patrascu L, Elisei AM, Segal R. The antioxidant activity of selected Romanian honeys. Ann University Dunarea de Jos of Galati Fasc VI Food Technol 2010;34:67-73.

  • 87. Piljac-Žegarac J, Stipčević T, Belščak A. Antioxidant properties and phenolic content of different floral origin honeys. J ApiProduct ApiMedical Sci 2009;1:43-50. doi: 10.3896/IBRA.4.01.2.04

  • 88. Radović DI, Lazarević KB, Trifković JĐ, Andrić FLj, Tešić ŽLj, Anđelković IB, Nedić NM, Stanimirović Z, Stevanović J, Ćurčić BPM, Milojković-Opsenica DM. GIS technology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia. Arch Biol Sci Belgrade 2014;66:935-46. doi: 10.2298/ABS1402935R

  • 89. Gašić U, Šikoparija B, Tosti T, Trifković J, Milojković- Opsenica D, Natić M, Tešić Ž. Phytochemical fingerprints of lime honey collected in Serbia. J AOAC Int 2014;97:1259-67. doi: 10.5740/jaoacint.SGEGasic

  • 90. Truchado P, Ferreres F, Tomas-Barberan FA. Liquid chromatography-tandem mass spectrometry reveals the widespread occurrence of flavonoid glycosides in honey, and their potential as floral origin markers. J Chromatogr A 2009;1216:7241-8. doi: 10.1016/j.chroma.2009.07.057

  • 91. Association of Analytical Communities (AOAC) official methods of analysis method 998.12: C-4 plant sugars in honey, internal standard stable carbon isotope ratio method. AOAC Int. Gaithersburg MD (USA). 1999, Chap. 44, p. 27-30.

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