In vitro cytotoxicity evaluation of different pulp capping materials: a comparative study

1. European Society of Endodontology. Quality guidelines for endodontic treatment: consensus report of the European Society of Endodontology. Int Endod J 2006;39:921-30. doi: 10.1111/j.1365-2591.2006.01180.x10.1111/j.1365-2591.2006.01180.x17180780Search in Google Scholar

2. Dominguez MS, Witherspoon DE, Gutmann JL, Opperman LA. Histological and scanning electron microscopy assessment of various vital pulp-therapy materials. J Endod 2003;29:324-33. PMID: 1277500410.1097/00004770-200305000-0000312775004Search in Google Scholar

3. Camilleri J, Pitt Ford TR. Mineral trioxide aggregate: a review of the constituents and biological properties of the mater ial . I n t Endod J 2006;39:747-54. doi: 10.1111/j.1365-2591.2006.01135.x10.1111/j.1365-2591.2006.01135.x16948659Search in Google Scholar

4. Desai S, Chandler N. Calcium hydroxide-based root canal sealers: a review. J Endodod 2009;39:415-22. doi: 10.1016/j. joen.2008.11.026Search in Google Scholar

5. Mohammadi Z, Dummer PMH. Properties and applications of calcium hydroxide in endodontics and dental traumatology. Int Endod J 2011 ; 40 : 697 - 730 . doi : 10.1111/j.1365-2591.2011.01886.x10.1111/j.1365-2591.2011.01886.x21535021Search in Google Scholar

6. Duda S, Dammaschke T. [Maßnahmen zur Vitalerhaltung der Pulpa. Gibt es Alternativen zum Kalziumhydroxid bei der direkten Überkappung, in German] Quintessenz 2008;59:1327-34.Search in Google Scholar

7. Goldberg M, Six N, Decup F, Lasfargues JJ, Salih E, Tompkins K, Veis A. Bioactive molecules and the future of pulp therapy. Am J Dent 2003;16:66-76. PMID: 12744417Search in Google Scholar

8. Almushayt A, Narayanan K, Zaki AE, George A. Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts. Gene Ther 2006;13:611-20. PMID: 1631994610.1038/sj.gt.330268716319946Search in Google Scholar

9. Takita T, Hayashi M, Takeichi O, Ogiso B, Suzuki N, Otsuka K, Ito K. Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells. Int Endod J 2006;39:415-22. PMID: 1664064210.1111/j.1365-2591.2006.01097.x16640642Search in Google Scholar

10. Gandolfi MG, Shah SN, Feng R, Prati C, Akintoye SO. Biomimetic calcium-silicate cements support differentiation of human orofacial bone marrow stromal cells. J Endodod 2011;37:1102-8. doi: 10.1016/j.joen.2011.05.00910.1016/j.joen.2011.05.009316383521763902Search in Google Scholar

11. Okiji T, Yoshiba K. Reparative dentinogenesis induced by mineral trioxide aggregate: a review from the biological and physicochemical points of view. Int J Dent 2009;2009:464280. doi: 10.1155/2009/46428010.1155/2009/464280283731420339574Search in Google Scholar

12. Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review part ii: leakage and biocompatibility investigations. J Endod 2013;36:190-202. doi: 10.1016/j.joen.2009.09.01010.1016/j.joen.2009.09.01020113774Search in Google Scholar

13. Moghaddame-Jafari S, Mantellini MG, Botero TM, McDonald NJ, Nör JE. Effect of ProRoot MTA on pulp cell apoptosis and proliferation in vitro. J Endod 2005;31:387-91. Doi: 10.1097/01.don.0000145423.89539.d710.1097/01.don.0000145423.89539.d715851935Search in Google Scholar

14. Hauman CHJ, Love RM. Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 2. Root canal-filling materials. Int Endod J 2003;36:147-60. PMID: 1265714010.1046/j.1365-2591.2003.00637.xSearch in Google Scholar

15. Sawicki L, Pameijer CH, Emerich K, Adamowicz-Klepalska B. Histological evaluation of mineral trioxide aggregate and calcium hydroxide in direct pulp capping of human immature permanent teeth. Am J Dent 2008;21:262-6. PMID: 18795524Search in Google Scholar

16. Asgary S, Shahabi S, Jafarzadeh T, Amini S, Kheirieh S. The properties of a new endodontic material. J Endod 2008;34:990-3. doi: 10.1016/j.joen.2008.05.00610.1016/j.joen.2008.05.006Search in Google Scholar

17. Gandolfi MG, Pagani S, Perut F, Ciapetti G, Baldini N, Mongiorgi R, Prati C. Innovative silicate-based cements for endodontics: a study of osteoblast-like cell response. J Biomed Mat Res A 2008;87:477-86. doi: 10.1002/ jbm.a.3179510.1002/jbm.a.31795Search in Google Scholar

18. Gomes-Filho JE, Rodrigues G, Watanabe S, Estrada Bernabé PF, Lodi CS, Gomes AC, Faria MD, Domingos Dos Santos A, Silos Moraes JC. Evaluation of the tissue reaction to fast endodontic cement (CER) and Angelus MTA. J Endod 2009;35:1377-80. doi: 10.1016/j.joen.2009.06.01010.1016/j.joen.2009.06.010Search in Google Scholar

19. Johnson BR. Considerations in the selection of a rootend filling material. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;87:398-404. PMID: 1022562010.1016/S1079-2104(99)70237-4Search in Google Scholar

20. Camilleri J. Characterization of hydration products of mineral trioxide aggregate. Int Endod J 2008;41:408-17. doi: 10.1111/j.1365-2591.2007.01370.x10.1111/j.1365-2591.2007.01370.x18298574Search in Google Scholar

21. Camilleri J. Characterization and hydration kinetics of tricalcium silicate cement for use as a dental biomaterial. Dent Mater 2011;27:836-44. doi: 10.1016/j. dental.2011.04.010Search in Google Scholar

22. Tay FR, Pashley DH, Rueggeberg FA, Loushine RJ, Weller RN. Calcium phosphate phase transformation produced by the interaction of the Portland cement component of white mineral trioxide aggregate with a phosphate-containing fluid. J Endod 2007;33:1347-51. doi: 10.1016/j.joen.2007.07.00810.1016/j.joen.2007.07.00817963961Search in Google Scholar

23. Taddei P, Inti A, Gandolfi MG, Possi PML, Prati C. Ageing of calcium silicate cements for endodontic use in simulated body fluids: a micro-Raman study. J Raman Spectrosc 2009;40:1858-66. doi: 10.1002/jrs.233310.1002/jrs.2333Search in Google Scholar

24. Han L, Okiji T, Okawa S. Morphological and chemical analysis of different precipitates on mineral trioxide aggregate immersed in different fluids. Dent Mat J 2010;29:512-7. doi: 10.4012/dmj.2009-13310.4012/dmj.2009-13320823620Search in Google Scholar

25. Babich H, Sinensky MC. Indirect cytotoxicity of dental materials: a study with Transwell inserts and the neutral red uptake assay. Altern Lab Anim 2001;29:9-13. PMID: 1117857110.1177/02611929010290010111178571Search in Google Scholar

26. Bogović A, Nižetić J, Galić N, Želježić D, Micek V, Mladinić M. The effects of hyaluronic acid, calcium hydroxide, and dentin adhesive on rat odontoblasts and fibroblasts. Arh Hig Rada Toksikol 2011;62:155-61. doi: 10.2478/10004-1254-62-2011-207610.2478/10004-1254-62-2011-2076Search in Google Scholar

27. Oran EO, Maden M, Senguuven B. Odontoblast-like cell numbers and reparative dentine thickness after direct pulp capping with platelet-rich plasma and enamel matrix derivative: a histomorphometric evaluation. Int Endod J 2012;45:317-25. doi: 10.1111/j.1365-2591.2011.01977.x10.1111/j.1365-2591.2011.01977.xSearch in Google Scholar

28. Maqbool F, Bahadar H, Abdollahi M. Exposure to mercury from dental amalgams: a threat to society. Arh Hig Rada Toksikol 2014;65:339-40. doi: 10.2478/10004-1254-65-2014-254310.2478/10004-1254-65-2014-2543Search in Google Scholar

29. Valey JW, Simonian PT, Conrad EU. Carcinogenicity and metallic implants. Am J Orthop 1995;24:319-24. PMID: 7788311Search in Google Scholar

30. Moodley D, Grobler SR, Olivler A. Cytotoxicity of a dentine bonding agent on four different cell-lines. SADJ 2005;60:234-6. PMID: 16119020Search in Google Scholar

31. Dianat O, Azadnia S, Mozayeni MA. Toxicity of calcium hydroxide nanoparticles on murine fibroblast cell line. Iran Endod J 2015;10:49-54. PMID: 25598810Search in Google Scholar

32. Lessa FC, Aranha AM, Nogueira I, Giro EM, Hebling J, Costa CA. Toxicity of chlorhexidine on odontoblast-like cells J Appl Oral Sci 2010;18:50-8. doi: 10.1590/S1678-77572010000100010 10.1590/S1678-77572010000100010Search in Google Scholar

33. Costa CAS, Vaerten MA, Edwards CA, Hanks CT. Cytotoxic effects of current dental adhesive systems on immortalized odontoblast cell line MDPC-23. Dent Mater 1999;15:434-41. doi: 10.1016/S0109-5641(99)00071-810.1016/S0109-5641(99)00071-8Search in Google Scholar

34. Ceci M, Beltrami R, Chiesa M, Colombo M, Poggio C. Biological and chemical-physical properties of root-end filling materials: A comparative study. J Conserv Dent 2015;18:94-9. doi: 10.4103/0972-0707.15305810.4103/0972-0707.153058Search in Google Scholar

35. Cavalcanti BN, Rode SM, Marques MM. Cytotoxicity of substances leached or dissolved from pulp capping materials. Int Endod J 2005;38:505-9. PMID: 1601176710.1111/j.1365-2591.2005.00967.xSearch in Google Scholar

36. Holland R, Souza V, Mello W, Nery MJ, Bernabé PF, Otoboni JA Filho. Permeability of the hard tissue bridge formed after pulpotomy with calcium hydroxide: a histologic study. J Am Dent Assoc 1979;99:472-5. PMID: 11213410.14219/jada.archive.1979.0317Search in Google Scholar

37. Goldberg F, Massone EJ, Spielberg C. Evaluation of the dentinal bridge after pulpotomy and calcium hydroxide dressing. J Endod 1984;10:318-20. doi: 10.1016/S0099-2399(84)80186-710.1016/S0099-2399(84)80186-7Search in Google Scholar

38. Asgary, S, Eghbal MJ, Parirokh M, Ghanavati F, Rahimi H. A comparative study of histologic response to different pulp capping materials and a novel endodontic cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:609-14. doi: 10.1016/j.tripleo.2008.06.00610.1016/j.tripleo.2008.06.00618718783Search in Google Scholar

39. Min KS, Kim HI, Park HJ, Pi SH, Hong CU, Kim EC. Human pulp cells response to Portland cement in vitro. J Endod 2007;33:163-6. doi: 10.1016/j.joen.2006.07.02210.1016/j.joen.2006.07.02217258637Search in Google Scholar

40. Zeferino EG, Bueno CE, Oyama LM, Ribeiro DA. Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide. Int Endod J 2010;43:843-8. doi: 10.1111/j.1365-2591.2010.01747.x 10.1111/j.1365-2591.2010.01747.x20722754Search in Google Scholar

41. Tabarsi B, Parirokh M, Eghbal MJ, Haghdoost AA, Torabzadeh H, Asgary S. A comparative study of dental pulp response to several pulpotomy agents. Int Endod J 2010;43:565-71. doi: 10.1111/j.1365-2591.2010.01711.x10.1111/j.1365-2591.2010.01711.x20456516Search in Google Scholar

42. Eskandarizadeh A, Shahpasandzadeh MH, Shahpasandzadeh M, Torabi M, Parirokh M. A comparative study on dental pulp response to calcium hydroxide, white and grey mineral trioxide aggregate as pulp capping agents. J Conserv Dent 2011;14:351-5. doi: 10.4103/0972 0707.87196Search in Google Scholar

43. Atmeh AR, Chong EZ, Richard G, Festy F, Watson TF. Dentin-cement interfacial interaction: calcium silicates and polyalkenoates. J Dent Res 2012;91:454-9. doi: 10.1177/002203451244306810.1177/0022034512443068407752722436906Search in Google Scholar

44. Grech L, Mallia B, Camilleri J. Characterization of set Intermediate Restorative Material, Biodentine, Bioaggregate and a prototype calcium silicate cement for use as root-end filling materials. Int Endod J 2013,46:632-41. doi: 10.1111/ iej.1203910.1111/iej.1203923289940Search in Google Scholar

45. About I. Dentin regeneration in vitro: the pivotal role of supportive cells. Adv Dent Res 2011;23:320-4. doi: 10.1177/002203451140532410.1177/002203451140532421677086Search in Google Scholar

46. Novicka A, Lipski M, Parafiniuk M, Sporniak-Tutak K, Lichota D, Kosierkiewicz A, Kaczmarek W, Buczkowska- Radlińska J. Response of human dental pulp capped with biodentine and mineral trioxide aggregate. J Endod 2013;39:743-7. doi: 10.1016/j.joen.2013.01.00510.1016/j.joen.2013.01.00523683272Search in Google Scholar

47. Zhou HM, Shen Y, Wang ZJ, Li L, Zheng YF, Häkkinen L, Haapasalo M. In vitro cytotoxicity evaluation of a novel root repair material. J Endod 2013;39:478-83. doi: 10.1016/j. joen.2012.11.026 Search in Google Scholar