Some of the genotoxic/carcinogenic substances or metabolites in cigarette smoke are capable of passing through the placenta and harming a newborn’s health. Smoking is also known as a factor in the formation of oxidative damage and the main mechanism involved in the carcinogenic process. Predetermining this genotoxic risk can be successfully achieved by measuring certain parameters of oxidative stress. The comet assay is considered an important biomarker for the evaluation of genotoxic substances and is effective for detecting DNA damage caused by smoking. This study examined third trimester bloods and the cord blood of 28 actively smoking and 22 non-smoking mothers in terms of DNA damage and oxidative stress parameters. Cu/Zn superoxide dismutase (CuZn-SOD), malondialdehyde (MDA), catalase (CAT), plasma nitrite/nitrates (NO2 -/NO3 -), selenium-dependent glutathione peroxidase (Se-GPx), Cu, and Zn levels were measured as indicators of oxidative damage. There were no significant increases in DNA damage of the actively smoking pregnant group in comparison with the non-smoking pregnant group, either in the third trimester or cord blood. Oxidative stress parameters of smoker and non-smoker groups were statistically different for MDA (p<0.05), CuZn-SOD (p<0.01), Se-GPx (p<0.05) values while the difference was not significant for NO2 -/NO3 -, CAT, Zn, and Cu values. The same values were also investigated in cord blood, and only NO2/NO3 -(p<0.01), Se-GPx (p<0.01 and CAT (p<0.001) values were found statistically different. Smoking mothers may have been exposed to more oxidative stress than non-smoking mothers.
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1. Katić J Fučıć A Gamulin M. Prenatal early life and childhood exposure to genotoxicants in the living environment. Arh Hig Rada Toksikol 2010;61:455-64.
2. Boffetta P Tredaniel J Greco A. Risk of childhood cancer and adult lung cancer after childhood exposure to passive smoke: A Meta-Analysis. Environ Health Perspect 2000;108:73-82.
3. de Assis KR Ladeira MS Bueno RC Dos Santos BF Dalben I Salvadori DM. Genotoxicity of cigarette smoking in maternal and newborn lymphocytes. Mutat Res 2009;679:72-8.
4. Shea AK Steiner M. Cigarette smoking during pregnancy. Nicotine Tob Res 2008;10:267-78.
5. Fowles J Dybing E. Application of toxicological risk assessment principles to the chemical constituents of cigarette smoke. Tob Control 2003;12:424-30.
6. Tsui H Wu HI Lin C Wang R Chiu H Cheng Y Chiu T Wu F. Prenatal smoking exposure and neonatal DNA damage in relation to birth outcomes. Pediatr Res 2008;64:131-4.
7. Adenuga D Rahman I. Oxidative stress histone deacetylase and corticosteroid resistance in severe asthma and COPD. Curr Res Med Rev 2007;3:57-68.
8. Aycicek A Ipek A. Maternal active or passive smoking causes oxidative stress in cord blood. Eur J Pediatr 2008;167:81-5.
9. Ermis B Ors R Yildirim A Tastekin A Kardas F Akcay F. Influence of smoking on maternal and neonatal serum malondialdehyde superoxide dismutase and glutathione peroxide levels. Ann Clin Lab Sci 2004;34:405-9.
10. Orhon FS Ulukol B Kahya D Cengiz B Başka S Tezcan S. The influence of maternal smoking on maternal and newborn oxidant and antioxidant status. Eur J Pediatr 2009;168:975-81.
11. Northrop-Clewes CA Thurnham DI. Monitoring micronutrients in cigarette smokers. Clin Chim Acta 2007;377:14-38.
12. Songul Şahinli A Marakoğlu K Kiyici A. Evaluation of the levels of oxidative stress factors and ischemia modified albumin in the cord blood of smoker and non-smoker pregnant women. J Matern Fetal Neonatal Med 2012;25:1064-8.
13. Lee BM Lee SK Kim HS. Inhibition of oxidative DNA damage 8-OHdG and carbonyl contents in smokers treated with antioxidants (vitamin E vitamin C beta-carotene and red ginseng). Cancer Lett 1998;132:219-27.
14. Bruin JE Gerstein HC Holloway AC. Long-term consequences of fetal and neonatal nicotine exposure: a critical review. Toxicol Sci 2010;116:364-74.
15. DeMarini DM. Genotoxicity of tobacco smoke and tobacco smoke condensate: a review. Mutat Res 2004;567:447-74.
16. Yang Q Hergenhahn M Weninger A Bartsch H. Cigarette smoke induces direct DNA damage in the human B-lymphoid cell line Raji. Carcinogenesis 1999;20:1769-75.
17. Fielding S Short C Davies K Wald N Bridges BA Waters R. Studies on the ability of smoke from different types of cigarettes to induce single-strand breaks in cultured human cells. Mutat Res 1989;214:147-51.
18. Nakayama T Kaneko M Kodama M Nagata C. Cigarette smoke induces DNA single-strand breaks in human cells. Nature 1985;314:462-4.
19. Leanderson P Tagesson C. Cigarette smoke-induced DNA damage in cultured human cells: role of hydroxyl radicals and endonuclease activation. Chem Biol Interact 1992;81:197-208.
20. Hoffmann H Hogel J Speit G. The effect of smoking on DNA effects in the comet assay: a meta-analysis. Mutagenesis 2005;20:455-66.
21. Ellahuene MF Perez-Alzola LP Farfan-Urzua M Gonzalez- Hormazabal P Garay M Olmedo MI Last JA. Preliminary evaluation of DNA damage related with the smoking habit measured by the comet assay in whole blood cells. Cancer Epidemiol Biomarkers Prev 2004;13:1223-9.
22. Singh NP Mccoy MT Tice RR Schneider EL. A simple technique for the quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1998;175:184-91.
23. Tice RR Agurell E Anderson D Burlinson B Hartmann A Kobayashi H Miyamae Y Rojas E Ryu JC Sasaki YF. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000;35:206-21.
24. Arsova-Sarafinovska Z Eken A Matevska N Erdem O Sayal A Savaşer A Banev S Petrovski D Dzikova S Georgiev V Sikole A Ozgok Y Suturkova L Dimovski AJ Aydın A. Increased oxidative/nitrosative stress and decreased antioxidant enzyme activities in prostate cancer. Clin Biochem 2009;42:1228-35.
25. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
26. Tracey WR Tse J Carter G. Lipopolysaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther 1995;272:1011-5.
27. Mucci LA Granath F Cnattingius S. Maternal smoking and childhood leukemia and lymphoma risk among 1440542 Swedish children. Cancer Epidemiol Biomarkers Prev 2004;13:1528-33.
28. Sasco AJ Vainio H. From in utero and childhood exposure to parental smoking to childhood cancer: a possible link and the need for action. Hum Exp Toxicol 1999;18:192-201.
29. Filippini G Farinotti M Ferrarini M. Active and passive smoking during pregnancy and risk of central nervous system tumors in children. Paediatr Perinat Epidemiol 2000;14:78-84.
30. Wu FY Wu HD Yang HL Kuo HW Ying JC Lin CJ Yang CC Lin LY Chiu TH Lai JS. Associations among genetic susceptibility DNA damage and pregnancy outcomes of expectant mothers exposed to environmental tobacco smoke. Sci Total Environ 2007;386:124-33.
31. DeMarini DM Preston RJ. Smoking while pregnant: transplacental mutagenesis of the fetus by tobacco smoke. JAMA 2005;293:1264-5.
32. Hoffmann H Isner C Hogel J Speit G. Genetic polymorphisms and the effect of cigarette smoking in the comet assay. Mutagenesis 2005;20:359-64.
33. Collins AR. The comet assay for DNA damage and repair: principles applications and limitations. Mol Biotechnol 2004;26:249-61.
34. Moller P Knudsen LE S Loft Wallin H. The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol Biomarkers Prev 2000;9:1005-15.
35. Baykal Y Yilmaz MI Celik T Gok F Rehber H Akay C Kocar IH. Effects of antihtpertensive agents alpha receptor blockers beta blockers angiotensin-converting enzyme inhibitors angiotensin receptor blockers and calcium channel blockers on oxidative stres. J Hypertens 2003;21:1207-11.
36. Gumus S Yucal O Gamsizkan M Eken A Deniz O Tozkoparan E Genc O Bilgic H. The role of oxidative stres and effect of alpha-lipoic acid in reexpansion pulmonary edema-an experimental study. Arch Med Sci 2010;6:848-53.
37. Yasar M Yıldız S Mas R Dundar K Yıldırım A Korkmaz A Akay C Kaymakcıoglu N Ozısık T Sen D. The effect of hyperbaric oxygen treatment on oxidative stres in experimental acute necrotizing pancreatitis. Physiol Res 2003;52:111-6.
38. Gitto E Reiter RJ Karbownik M. Causes of oxidative stress in the pre-and perinatal period. Biol Neonate 2002;81:146-57
39. Yorbik O Sayal A Akay C Akbiyik DI Sohmen T. Investigation of antioxidant enzymes in children with autistic disorder. Prostaglandins Leukot Essent Fatty Acids 2002;67:341-3.
40. Bolcal C Yıldırım V Doğancı S Sargın M Aydın A Eken A Ozal E Kuralay E Demirkılıc U Tatar H. Protective effects of antioxidant medications on limb ischemia reperfusion injury. J Surg Res 2007;139:274-9.
41. Bolisetty S Naidoo D Lui K Koh TH Watson D Montgomery R Whitehall J. Postnatal changes in maternal and neonatal plasma antioxidant vitamins and the influence of smoking. Arch Dis Child Fetal Neonatal Ed 2002;86: F36-40.
42. Chelchowska M Laskowska-Klita T Niemiec KT. [Activities of superoxide dismutase glutathione peroxidase and catalase in erythrocytes of women smoking during pregnancy in Polish]. Przegl Lek 2005;62:1039-42.
43. Chelchowska M Laskowska-Klita T Leibschang J. [Activities of superoxide dismutase catalase glutathione peroxidase and reductase in umbilical cord blood of newborn from mothers smoking during pregnancy in Polish]. Przegl Lek 2006;63:970-3.
44. Schwarz KB Cox JM Sharma S Clement L Witter F Abbey H Sehnert SS Risby TH. Prooxidant effects of maternal smoking and formula in newborn infants. J Pediatr Gastroenterol Nutr 1997;24:68-74.
45. Serdar Z Dirican M Serdar A Sarandol E Yeşilbursa D Tokullugil A. Investigation of the effects of smoking on lipid peroxidation and antioxidants in patients with coronary artery disease. Turkiye Klinikleri J Med Sci 1999;19:266-74.
46. Walsh SW Wang Y. Secretion of lipid peroxides by the human placenta. Am J Obstet Gynecol 1993;169:1462-6.
47. Hulea SA Olinescu R Nita S Crocnan D Kummerow FA. Cigarette smoking causes biochemical changes in blood that are suggestive of oxidative stress: a case-control study. J Environ Pathol Toxicol Oncol 1995;14:173-80.
48. Kim SH Kim JS Shin HS Keen CL. Influence of smoking on markers of oxidative stress and serum mineral concentrations in teenage girls in Korea. Nutrition 2003;19:240-3.
49. Wurzel H Yeh CC Gairola C Chow CK. Oxidative damage and antioxidant status in the lungs and bronchoalveolar lavage fluid of rats exposed chronically to cigarette smoke. J Biochem Toxicol 1995;10:11-7.
50. Gupta MP Khanduja KL Sharma RR. Effect of cigarette smoke inhalation on antioxidant enzymes and lipid peroxidation in the rat. Toxicol Lett 1998;41:107-14.
51. Abou-Seif MA. Blood antioxidant status and urine sulfate and thiocyanate levels in smokers. J Biochem Toxicol 1996;11:133-8.
52. Hilbert J Mohsenin V. Adaptation of lung antioxidants to cigarette smoking in humans. Chest 1996;110:916-20.
53. Toda N Toda H. Nitric oxide-mediated blood flow regulation as affected by smoking and nicotine. Eur J Pharm 2010;649:1-13.
54. Wei T Chen C Hou J Xin W Mori A. Nitric oxide induces oxidative stress and apoptosis in neuronal cells. Biochim Biophys Acta 2000;1498:72-9.
55. Aydın A Orhan H Sayal A Ozata M Şahin G Işımer A. Oxidative stress and nitric oxide related parameters in type II diabetes mellitus: effects of glycemic control. Clin Biochem 2001;34:65-70.
56. Andersen MR Walker LR Stender S. Reduced endothelial nitric oxide synthase activity and concentration in fetal umbilical veins from maternal cigarette smoking. Am J Obstet Gynecol 2004;191:346-51.
57. Andersen MR Simonsen U Uldbjerg N Aalkjaer C Stender S. Smoking cessation early in pregnancy and birth weight length head circumstance and endothelial nitric oxide synthase activity in umbilical and chorionic vessels: An observational study of healthy singleton pregnancies. Circulation 2009;119:857-64.
58. Dani C Giannini L Bertini G Pratesi S Corsini I Longini M Buonocore G Masini E Rubaltelli FF. Changes of nitric oxide carbon monoxide and oxidative stress in term infants at birth. Free Radic Res 2007;41:1358-63.
59. Keen CL Clegg MS Ferrell F Hunter GC Dubick MA. Hypertension induced alterations in copper and zinc metabolism: a link to vascular disease? In: Sorenson JRJ editors. Biology of copper complexes. Clifton (NJ): Humana Press; 1987. p. 141-50.
60. Dubick MA Keen CL. Influence of nicotine on tissue trace element concentrations and tissue antioxidant defense. Biol Trace Elem Res 1991;31:97-109.