In Vitro Genotoxicity of Settat Town Landfill Leachate, Morocco

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In Vitro Genotoxicity of Settat Town Landfill Leachate, Morocco

With the increasing use of landfill sites, leachates produced by uncontrolled waste disposal have became a serious threat for the aquatic environment. The aim of this study was to evaluate the genotoxicity of leachate and of well water sampled close to the town of Settat in Morocco using the micronucleus test and proliferation kinetics of human peripheral blood lymphocytes in vitro. We also analysed a number of physical and chemical parameters, including pH, % O2, chemical oxygen demand (COD), HCO3-, Ca2+, Mg2+, Cl-, and conductivity.

The analysis showed much higher levels of nearly all parameters than the Moroccan standard. Increased micronucleus frequencies were also found for both leachate and well water. Preliminary results indicate that both types of water are genotoxic and pose environmental and human health risk.

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  • Cabrera GL Rodriguez DMG. Genotoxicity of leachates from a landfill using three bioassays. Mutat Res 1999;426:207--10.

  • Jupsin H Praet E Vasel J-L. Caractérisation des lixiviats de CET et modélisation de leur évolution [Sanitary landfill characterization and modelisation of their evolution in French]. In: Proceedings of the International Symposium on Environmental Pollution Control and Waste Management 7--10 January 2002 Tunis (EPCOWM'2002) [displayed 25 May 2009]. Available at

  • Christensen H Kjeldsen P Albrechtsen H Heron G Nielsen PH Bjerg PL Holm PE. Attenuation of landfill leachate pollutants in ground water. Crit Rev Environ Sci Technol 1994;24:119--202.

  • Halim CE Amal R Beydoun D Scott JA Low G. Evaluating the applicability of regulatory leaching tests for assessing the hazards of Pb-contaminated soils. J Hazard Mater 2005;B120:101--11.

  • Omura M Inamasu T Ishinishi N. Mutagenic activity of the leachate of municipal solid waste landfill. Mutat Res 1992;298:125--9.

  • Schrab GE Brown KW Donnelly KC. Acute and genetic toxicity of municipal landfill leachate. Water Air Soil Poll 1993;69:99--112.

  • Gonsebatt ME Salazar AM Montero R Díaz Barriga F Yáñez L Gómez H Ostrosky-Wegman P. Genotoxic monitoring of workers at a hazardous waste disposal site in Mexico. Environ Health Persp 1995;103(Suppl 1):111--3.

  • Fang M Liu D Kuang Y. Micronucleus effects of refuse leachate on root tip cells of Vicia faba.Chin J Appl Environ Biol 1999;5:574--7.

  • Li G Sang N Zhao Y. Micronuclei induced by municipal landfill leachate in mouse bone marrow cells in vivo. Environ Res 2004;95:77--81.

  • Li G Yun Y Li H Sang N. Effect of landfill leachate on cell cycle micronucleus and sister chromatid exchange in Triticum saestivum.J Hazard Mater 2008;155:10--6.

  • Chandra S Chauhan LK Pande PN Gupta SK. Cytogenetic effects of leachates from tannery solid waste on the somatic cells of Vicia faba.Environ Toxicol 2004;19:129--33.

  • Lah N Vidic T Glasencnik E Cepeljnik T Gorjanc G Marinsek-Logar R. Genotoxicity evaluation of water soil leachates by Ames test comet assay and preliminary Tradescantia micronucleus assay. Environ Monit Assess 2008;139:107--18.

  • Brown KW Donnelly KC. Mutagenic activity of runoff and leachate water from hazardous waste land treatment. Environ Poll (Series A) 1984;35:229--46.

  • Picado A Mendonça E Silva L Paixão SM Brito F Cunha MA Leitão S Moura I Hernan R. Ecotoxicological assessment of industrial wastewaters in Trancão river basin (Portugal). Environ Toxicol 2008;23:466--72.

  • Association Française de Normalisation (AFNOR). Qualité de l'eau. Tome 1: Terminologie échantillonnage et évaluation des méthodes [Water quality. Volume 1: Terminology sampling and evaluation methods; in French]. 6th ed. Paris: AFNOR; 2001.

  • Rodier J Bazin C Broutin JP Chambon P Champsaur H Rodi L editors. L'analyse de l'eau: Eaux naturelles eaux résiduaires eaux de mer: chimie physico-chimie microbiologie biologie interprétation des résultats [An analysis of water: natural waters wastewater water wednesday in French]. 8th ed. Paris: DUNOD; 1996.

  • Fenech M Morley AA. Measurement of micronuclei in lymphocytes. Mutat Res 1985;147:29--36.

  • Fenech M. The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations. Mutat Res 1993;285:35--44.

  • Titenko-Holland N Windhan G Kolachana P Reinish F Paravatham S Osorio AM. Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: a study of malathion-exposed workers. Mutat Res 1997;388:85--95.

  • Sang N Li G. Genotoxicity of municipal landfill leachate on root tips of Vicia faba.Mutat Res 2004;560:159--65.

  • Kjeldsen P. Groundwater pollution source characterization of an old landfill. J Hydrol 1993;142:349--71.

  • Bonassi S Znaor A Ceppi M Lando C Chang WP Holland N Kirsch-Volders M Zeiger E Ban S Barale R Bigatti MP Bolognesi C Cebulska-Wasilewska A Fabianova E Fucic A Hagmar L Joksic G Martelli A Migliore L Mirkova E Scarfi MR Zijno A Norppa H Fenech M. An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 2007;28:625--31.

  • Durgo K Oreščanin V Luliċ S Kopjar N Želježić D Franekić Čolić J. The assessment of genotoxicity effects of wastewater from a fertilized factory. J Appl Toxicol 2008;29:42--51.

  • Fenech M. Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability mitotic dysfunction and cell death. Mutat Res 2006;600:58--66.

  • Roser S Pool-Zobel BL Rechkemmer G. Contribution of apoptosis to responses in the comet assay. Mutat Res 2001;497:169--75.

  • Sang N Li G Xin X. Municipal landfill leachate induces cytogenetic damage in root tips of Hordeum vulgare.Ecotox Environ Safety 2006;63:469--73.

  • Baun A Ledin A Reitzel LA Bjerg PL Christensen TH. Xenobiotic organic compounds in leachates from ten Danish MSW landfills - chemical analysis and toxicity tests. Water Res 2004;38:3845--58.

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