The influence of a Copper-Containing Funcicide on the Gametophyte of Some Non-Target Pteridophyte Species

Open access

Abstract

The aim of this study was to assess the effects of a fungicide with 20% metallic copper content on spore germination and gametophyte development in two non-target pteridophyte species: Asplenium scolopendrium and Athyrium filix-femina. The experimental variants were: V1 - 0.1% fungicide in Knop solution, V2 - 0.5% fungicide in Knop solution, V3 - 0.7% fungicide in Knop solution, and the Control variant (C) - Knop solution. The fungicide used affected spore germination in all the tested variants. The lowest germination percentages were registered in the species Asplenium scolopendrium: V1 - 69.33%, V2 - 65.66%, V3 - 51.33%. In terms of gametophyte differentiation, the experiments led to delays in developmental stages, absence of rhizoids and necrosis of prothallic cells. The results of the study can be used to assess the impact of the fungicide on the ecosystems in which it is applied, ecosystems where pteridophytes are also present or ecosystems adjacent to them.

References
  • Cassanego, M. B. B., Droste, A. & Windisch, P. G. (2010). Effects of 2,4-D on the germination of megaspores and initial development of Regnellidium diphyllum Lindm. (Monilophyta, Marsileaceae), Braz. J. Biol., 70(2), 361-366.

  • Catalá, M., Esteban, M. & Quintanilla, L. G. (2011). Mitochondrial Activity of Fern Spores for the Evaluation of Acute Toxicity in Higher Plant Development. In H. Fernández, A. Kumar & M.A. Revilla (Eds). Working with Ferns, Issues andApplications (pp. 237-247). New-York: Springer Science+Business Media Springer LLC.

  • Droste, A., Cassanego, M. B. B. & Windisch P. G. (2010). Germination and sporophytic development of Regnellidium diphyllum Lindm. (Marsileaceae) in the presence of a glyphosate-based herbicide. Braz. J. Biosc., 8(2), 174-178.

  • Ehrendorfer, F. (1999). Pteridophyta. In P. Sitte, H. Ziegler, F. Ehrendorfer, A. Bresinsky (Eds). Strasburger - Lehrbuch der botanik (pp. 652-684). Berlin: Spektrum Akademischer Verlag Heidelberg.

  • Evans, L. S. & Bozzone, D. M. (1978). Effect of buffered solutions and various anions on vegetative development and sexual development in gametophytes of Pteridium aquilinum. Can. J. Bot., 56, 779-785.

  • Fernández, H., Bertrand, A. M. & Sánchez-Tamés, R. (1999). Biological and nutritional aspect involved in fern multiplication. Plant Cell Tiss. Org., 56, 211-214.

  • Fernández, H. & Revilla, M. A. (2003). In vitro culture of ornamental ferns. Plant CellTiss. Org., 73, 1-13.

  • Gumaelius, L., Lahner, B., Salt, D. E. & Banks, J. A. (2004). Arsenic hyperaccumulation in gametophytes of Pteris vittata: A new model system for analysis of arsenic hyperaccumulation. Plant Physiol., 136, 1-11.

  • Kamachi, H., Komori, I., Tamura, H., Sawa, Y., Karahara, I., Honma, Y., Wada, N., Kawabata, T., Matsuda, K., Ikeno, S., Noguchi, M. & Inoue, H. (2005). Lead tolerance and accumulation in the gametophytes of the fern Athyriumyokoscense. J. Plant Res., 118, 137-145.

  • Keary, P. I., Thomas, C. & Sheffield, E. (2000). The effect of the herbicide Asulam on the gametophytes of Pteridium aquilinum, Cryptogramma crispa and Dryopterisfilix-mas. Ann. of Bot., 85, 47-51.

  • Knopp, W. (1865). Quantitative Untersuchugen uber die Ernahrungsprozesse der Pflanzen. Landwintsch Vers Stn., 7, 93-107.

  • Luo, X-Y. & Ikeda, H. (2007). Effects of four rice herbicides on the growth of an aquatic fern, Marsilea quadrifolia L.. Weed Biol. and Manag., 7(4), 237-241.

  • Mohr, H. (1963). The influence of visible radiation on the germination of archegoniate spores and the growth of the fern protonema. J. Linn. Soc. (Bot.), 58(373), 287-296.

  • Muccifora, S. (2008). Effects of copper on spore germination, growth and ultrastructure of Polypodium cambricum L. gametophytes. Environ. Pollut., 153(2), 369-375.

  • Nishizono, H., Suzuki, S. & Ishii, F. (1987a). Accumulation of heavy metals in the metal-tolerant fern Athyrium yokoscense, growing on various environments. Plant and Soil, 102(1), 65-70.

  • Nishizono, H., Ichikawa, H., Suziki, S. & Ishii, F. (1987b). The role of the root cell wall in the heavy metal tolerance of Athyrium yokoscense. Plant and Soil, 101(1), 15-20.

  • Pangua, E., Lindasay, S., Dyer, A. (1994). Spore germination and gametophyte development in three species of Asplenium. Ann. of Bot., 73(6), 587-593.

  • Pangua, E., Belmonte, R. & Pajaron S. (2009). Germination and reproductive biology in salty conditions of Asplenium marianum (Aspleniaceae), a european coastal fern. Flora, 204, 673-684.

  • Sela, M., Jacob, G. & Tel-Or, E. (1989). The accumulation and the effect of the heavy metals on the water fern Azolla filiculoides. New Phytol., 112(1), 7-12.

  • Sheffield, E. (2002). Effects of Asulam on non-target pteridophytes. Fern. Gaz., 16, 377-382.

  • Sugai, M., Tomizava, K., Watanabe, M. & Furuya, M. (1984). Action spectrum between 250 and 800 nanometters for the photoinduced inhibition of spore germination in Pteris vittata. Plant Cell Physiol., 25, 205-212.

  • Tomizawa, K., Sugai, M. & Manake, K. (1983). Relationship between germination and Pfr level in spores of the fern Lygodium japonicum. Plant Cell Physiol., 24, 1043-1048.

Acta Horti Botanici Bucurestiensis

The Journal of University of Bucharest

Journal Information

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 10 10 10
PDF Downloads 2 2 2