Methods for determination of labile soil organic matter: An overview
Soil organic matter (SOM) can be divided into three main pools: labile, stable and inert. Research over recent years has focused on the labile fraction (LF), as it is considered a quickly reactive indicator of soil productivity and health, and important as a supply of energy for soil micro-organisms. A wide spectrum of analytical methods has been used to determine and/or evaluate LF, based on physical, chemical and biochemical principles. The advantages and disadvantages of each technique are explored in this work, but none of the methods can determine LF sufficiently, either because a part of the LF is not involved or because further characterisation is missing. Although analytical methods are widely used to evaluate changes in soil management or organic carbon turnover, the practical question of the quantity and quality of SOM cannot be answered completely. It is also suggested that future research should focus on the interactions among SOM fractions and their better chemical and functional characterisation. It is possible to use a combination of the analytical methods reviewed here in order to accomplish this objective.
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Adamczyk B Kitunen V Smolander A (2009): Polyphenol oxidase tannase and proteolytic activity in relation to tannin concentration in the soil organic horizon under silver birch and Norway spruce. Soil Biol Biochem 41: 2085-2093.
Alexandrovová LN (1970): About nomenclature of soil humus matter. In Soil humus (Its genesis properties and importance for pedogenesis and soil productivity). Biol Nauki Zap LSCHI p. 91-99 (in Russian).
Alvarez CR Alvarez R Grigera S Lavado RS (1998): Associations between organic matter fractions and the active soil microbial biomass. Soil Biol Biochem 30: 767-773.
Balaria A Johnson CE Xu ZH (2009): Molecularscale characterization of hot-water-extractable organic matter in organic horizons of a forest soil. Soil Sci Soc Am J 73: 812-821.
Baldock JA (2007): Composition and cycling of organic carbon in soil. In Marschner P Rengel Z (eds.): Nutrient ycling in terrestrial ecosystems. London Springer pp. 1-35.
Barriuso E Portal JM Andreux F (1987): Kinetics and mechanisms of the acid-hydrolysis of organic-matter in a humic-rich mountain soil. Can J Soil Sci 67: 647-658.
Berg B Johansson MB Nilsson A (2009): Sequestration of carbon in the humus layer of Swedish forests - direct measurements. Can J For Res 39: 962-975.
Biederbeck VO Janzen HH Campbell CA Zentner RP (1994): Labile soil organic-matter as influenced by cropping practices in an arid environment. Soil Biol Biochem 26: 1647-1656.
Blair GJ Lefroy RDB Lise L (1995): Oxidation and the development of a carbon management index for agricultural systems. Aust J Agr Res 46: 1459-1466.
Blair N (2006): Long-term management impacts on soil C N and physical fertility - Part II: Bad Lauchstadt static and extreme FYM experiments. Soil Tillage Res 91: 39-47.
Bongiovanni MD Lobartini JC (2006): Particulate organic matter carbohydrate humic acid contents in soil macro- and microaggregates as affected by cultivation. Geoderma 136: 660-665.
Broos K Macdonald LM Warne MSJ Heemsbergen DA Barnes MB Bell M McLaughlin MJ (2007): Limitations of soil microbial biomass carbon as an indicator of soil pollution in the field. Soil Biol Biochem 39: 2693-2695.
Cambardella CA Elliot ET (1993): Methods for physical separation and characterization of soil organic-matter fractions. Geoderma 56: 449-457.
Capriel P (1997): Hydrophobicity of organic matter in arable soils: Influence of management. Eur J Soil Sci 48: 457-462.
Carter MR (1986): Microbial biomass as an index for tillage-induced changes in soil biological properties. Soil Tillage Res 7: 29-40.
Carter MR (2002): Soil quality for sustainable land management: Organic matter and aggregation interactions that maintain soil functions. Agron J 94: 38-47.
Chan KY Bowman A Oates A (2001): Oxidizable organic carbon fractions and soil quality changes in an oxic Paleustalf under different pature leys. Soil Sci 166: 61-67.
Chatterjee A Lal R (2009): On farm assessment of tillage impact on soil carbon and associated soil quality parameters. Soil Tillage Res 104: 270-277.
Dell CJ (2009): Potential for quantification of biologically active soil carbon with potassium permanganate. Commun Soil Sci Plant Anal 40: 1604-1609.
Flaig W Beutelspacher H Rietz E (1975): Chemical composition and physical properties of humic substances. In Gieseking JE (ed.): Soil Components Vol. 1: Organic Components Berlin Springer pp. 1-211.
Ghani A Dexter M Perrott KW (2003): Hot-water extractable carbon in soils; a sensitive measurment for determning impacts of fertilisation grazing and cultivation. Soil Biol Biochem 35: 1231-1243.
Giesler R Lundström U (1993): Soil solution chemistry: Effects of bulking soil samples. Soil Sci Soc Am J 57: 1283-1288.
Gregorich EG Ellert BH (1993): Light fraction and macroorganic matter in mineral soils. In Carter MR (ed.): Soil Sampling and Methods of Analysis. Ottawa Lewis Publisher pp. 397-406.
Gregorich EG Beare MH Stoklas V St Georges P (2003): Biodegrability of soluble organic matter in maize-cropped soils. Geoderma 113: 237-252.
Gregorich EG Beare MH Mckim UF Skjemstad JO (2006): Chemical and biological characteristics of physically uncomplexed organic matter. Soil Sci Soc Am J 70: 975-985.
Haynes RJ (2005): Labile organic matter fractions as central components of the quality of agricultural soils. Adv Agron 85: 221-268.
Heitkamp F Raupp J Ludwig B (2009): Impact of fertilizer type and rate on carbon and nitrogen pools in a sandy cambisol. Plant Soil 319: 259-275.
Helfrich M Flessa H Dreves A Ludwig B (2009): Is thermal oxidation at different temperatures suitable to isolate soil organic carbon fractions with different turnover? J Plant Nutr Soil Sci 173: 61-66.
Hilli S Stark S Derome J (2008): Water-extractable organic compounds in different components of the litter layer of boreal coniferous forest soils along a climatic gradient. Boreal Env Res 13: 92-106.
Jabro JD Sainju U Stevens WB (2008): Carbon dioxide flux as affected by tillage and irrigation in soil converted from perennial forages to annual crops. J Environ Manage 88: 1478-1484.
Jones DL Willett WB (2005): Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil. Soil Biol Biochem 38: 991-999.
Kasozi GN Nkedi-Kizza P Harris WG (2009): Varied carbon content of organic matter in histosols spodosols and carbonatic soils. Soil Sci Soc Am J 73: 1313-1318.
Keeney DR Bremner JM (1966): Comparison and evaluation of laboratory methods of obtaining an index of soil nitrogen availability. Argon J 58: 498-503.
King JA Bradley RI Harrison R (2005): Current trends of soil organic carbon in English arable soils. Soil Use Manage 21: 189-195.
Kolář L Klimeš F Ledvina R Kužel S (2003): A method to determine mineralization kinetics of decomposable part of soil organic matter in the soil. Plant Soil Environ 49: 8-11.
Kolář L Ledvina R Kužel S Klimeš F Štindl P (2006): Soil organic matter and its Stability in aerobic and anaerobic conditions. Soil Water Res 1: 57-64.
Kolář L Kužel S Horáček J Čechová V Borová-Batt J Peterka J (2009): Labile fractions of soil organic matter their quantity and quality. Plant Soil Environ 55: 245-251.
Kononovová MM (1963): Soil organic matter. Its nature properties and research methods. Moskva Izd. Nauka 314 p (in Russian).
Kononovová MM Belčiková NP (1961): Prompt methods for identification of humus composition in mineral soils. Počvovedenije 10: 75-87 (in Russian).
Körschens M (1980): Relations between the share of fine particles Ct and Nt Contents in the soil. Arch Acker Pflanzenb Bodenkunde 24: 585-592 (in German).
Körschens M Schulz E Behm R (1990): Hot water extractable carbon and nitrogen of soils as a criterion for their ability of N-release. Zbl Mikrobiol 145: 305-311 (in German).
Krull ES Swanston CW Skjemstad JO (2006): Importance of charcoal in determining the age and chemistry of organic carbon in surface soils. J Geophys Res Biogeosci 111: G04001 doi:10.1029/2006JG000194.
Kubát J Nováková J Mikanová O Apfelthaler R (1999): Organic carbon cycle incidence of microorganisms and respiration activity in long-term field experiment. Rostl Výr 45: 389-395.
Laik R Kumar K Das DK Chaturvedi OP (2009): Labile soil organic matter pools in a calciorthent after 18 years of afforestation by different plantations. Appl Soil Ecol 42: 71-78.
Lee SB Lee CH Jung KY Do Park K Lee D Kim PJ (2009): Changes of soil organic carbon and its fractions in relation to soil physical properties in a long-term fertilized paddy. Soil Tillage Res 104: 227-232.
Leifeld J Kogel-Knabner I (2001): Organic carbon and nitrogen in fine soil fractions after treatment with hydrogen peroxide. Soil Biol Biochem 33: 2155-2158.
Lopez-Fando C Pardo MT (2009): Changes in soil chemical characteristics with different tillage practices in a semi-arid environment. Soil Tillage Res 104: 278-284.
Loveland P Webb J (2003): Is there a critical level of organic matter in the agricultural soils of temperate regions: a review. Soil Tillage Res 70: 1-18.
Majumder B Mandal B Bandyopadhyay PK Chandhury J (2007): Soil organic carbon pools and productivity relationship for a 34 year old rice - wheat - jute agroecosystem under different fertilizer treatments. Plant Soil 297: 53-67.
Majumder B Mandal B Bandyopadhyay PK (2008): Soil organic carbon pools and productivity in relation to nutrient management in a 20-year-old rice-berseem agrosystem. Biol Fertil Soils 44: 451-461.
Melero S Lopez-Garrido R Murillo JM Moreno F (2009): Conservation tillage: Short- and long-term effects on soil carbon fractions and enzymatic activities under Mediterranean conditions. Soil Tillage Res 104: 292-298.
Mendham DS O'Connell AM Grove TS (2002): Organic matter characteristics under native forest log-term pasture and recent conversion to Eucalyptus plantations in Western Australia: microbial biomass soil respiration and permanganate oxidation. Aust J Soil Res 40: 859-872.
Mills AJ Fey MV (2004): Frequent fires intensify soil crusting: physicochemical feedback in the pedoderm of long-term burn experiments in South Africa. Geoderma 121: 45-64.
Mullen RW Thomason WE Raun WR (1999): Estimated increase in atmospheric carbon dioxide due to worldwide decrease in soil organic matter. Commun Soil Sci Plant Anal 30: 1713-1719.
Najmr S (1958): System of soil organic matter classification. Rostl Výr 31: 661-692 (in Czech).
Novák B (1965): The use of biochemical tests in soil microbiology. Doctoral thesis VŠZ Praha (in Czech).
Novák B (1966): The relation between composition- and energy-turnover of organic matter during humification. Rostl Výr 12: 709-711 (in Czech).
Novák B Apfelthaler R (1964): Contribution to methodology of respiration determination as an indicator of microbial processes in soil. Rostl Výr 10: 145-150 (in Czech).
Oades JM (1984): Soil organic matter and structural stability: mechanisms and implications for management. Plant Soil 76: 319-337.
Pajares S Gallardo JF Masciandaro G (2009): Biochemical indicators of carbon dynamic in an Acrisol cultivated under different management practices in the central Mexican highlands. Soil Tillage Res 105: 156-163.
Paul EA (1984): Dynamics of organic matter in soils. Plant Soil 76: 275-285.
Piccolo A (2002): The supramolecular structure of humic substances: A novel understanding of humus chemistry and implications in soil science. Adv Agron 75: 57-134.
Plante AF Conant RT Paul EA Paustian K Six J (2006): Acid hydrolysis of easily dispersed and microaggregate-derived silt- and claysized fractions to isolate resistant soil organic matter. Eur J Soil Sci 57: 456-467.
Poirier N Sohi SP Gaunt JL Mahieu N Randall EW Powlson DS Evershed RP (2005): The chemical composition of measurable soil organic matter pools. Org Geochem 36: 1174-1189.
Prechtel A von Lutzow M Schneider BU (2009): Organic carbon in soils of Germany: Status quo and the need for new data to evaluate potentials and trends of soil carbon sequestration. J Plant Nutr Soil Sci 172: 601-614.
Rovira P Vallejo VR (2000): Examination of thermal and acid hydrolysis procedures in characterization of soil organic matter. Commun Soil Sci Plant Anal 31: 81-100.
Rovira P Vallejo VR (2003): Physical protection and biochemical quality of organic matter in Mediterranean calcareous forest soils: a density fractionation approach. Soil Biol Biochem 35: 245-261.
Scheffer F Ulrich B (1960): Humus and organic manuring. Morphology biology chemistry and dynamic of humus. Enke Stuttgart (in German).
Schulten HR Leinweber P (1999): Thermal stability and composition of mineral-bound organic matter in density fractions of soil. Eur J Soil Sci 50: 237-248.
Schulz E Klimanek BM (1988): Transformation of organic nitrogen during decomposition of primary organic-matter (POM) in soil using N-15-tracer technique in incubation experiments and 1st results on C/N-transformation during decomposition of POM. Zbl Mikrobiol 143: 435-439.
Shirato Y Yokozawa M (2006): Acid hydrolysis to partition plant material into decomposable and resistant fractions for use in the Rothamsted carbon model. Soil Biol Biochem 38: 812-816.
Siewert C (1989): Mineralization dynamics of humus acids of a humic podzol. Arch Agron Soil Sci 33: 261-266.
Silveira ML Comerford NB Reddy KR Cooper WT El-Rifai H (2008): Characterization of soil organic carbon pools by acid hydrolysis. Geoderma 144: 405-414.
Skjemstad JO Swift RS McGowan JA (2006): Comparison of the particulate organic carbon and permanganate oxidation methods for estimating labile soil organic carbon. Aust J Soil Res 44: 255-263.
Sparling GP (1992): Ratio of microbial biomass carbon to soil organic-carbon as a sensitive indicator of changes in soil organic-matter. Aust J Soil Res 30: 195-207.
Stevenson FJ (1982): Humus Chemistry. New York Wiley.
Stevenson FJ (1994): Humus Chemistry: Genesis Composition Reactions. New York Wiley.
Stewart CE Paustian K Conant RT Plante AF Six J (2008): Soil carbon saturation: Evaluation and corroboration by long-term incubations. Soil Biol Biochem 21: 189-195.
Strosser E (2008): Soil organic matter evaluation system based on hydrophilic fractionation and characterization of the fractions with differential thermic analysis. Dipoloma thesis ZF JU České Budějovice (in Czech).
Tirol-Padre A Ladha J K (2004): Assessing the reliability of permanganate-oxidizable carbon as an index of soil labile carbon. Soil Sci Soc Am J 68: 969-978.
Tjurin IV (1937): Soil organic matter and its role in pedogenesis and soil productivity. Study of soil humus. Moskva Seľskozgiz (in Russian).
Tjurin IV (1951): Several results of study comparing humus composition in USSR soils. Trudy Počv Inst 38: 22-32 (in Russian).
Vance ED Brookes PC Jenkinson DS (1987): An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19: 703-707.
Walkley A Black IA (1934): An examination of Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37: 29-38.
Wang XL Jia Y Li XG (2009): Effects of land use on soil total and light fraction organic and microbial biomass C and N in a semi-arid ecosystem of northwest China. Geoderma 153: 285-290.
Xavier FAD Maia SMF Oliveira TS (2009): Soil organic carbon and nitrogen stocks under tropical organic and conventional cropping systems in Northeastern Brazil. Commun Soil Sci Plant Anal 40: 2975-2994.
Zimmermann M Leifeld J Abiven S Schmidt MWI Fuhrer J (2007): Sodium hypochlorite separates an older soil organic matter fraction than acid hydrolysis. Geoderma 139: 171-179.
Zsolnay A Gorlitz H (1994): Water-extractable organic-matter in arable soils - effects of drought and long-term fertilization. Soil Biol Biochem 26: 1257-1261.