Blood profile and gut microbial load of broilers fed siam weed (Chromolaena odorata) leaf meal in their diets

Open access


The responses of 96 day old broilers fed Chromolaena odorata leaf meal (COLM) on blood profile and gut microbial load were studied for 49 days. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The birds were fed four experimental diets formulated at 0%, 2%, 4% and 6% for the starter phase and 0%, 4%, 8% and 12% of COLM at the finisher phase for T1, T2, T3 and T4 respectively. All the haematological parameters were similar (P>0.05) across the treatment groups except MCH and WBC which were significantly (P< 0.05) higher and better in the treatment groups than the control group. The results on serum chemistry showed significant (P<0.05) difference for total protein, albumin, creatinine and cholesterol across the treatment groups. Cholesterol was significantly (P<0.05) reduced with increasing levels of COLM. Results showed that coliforms and E.coli were consistently higher (P<0.05) among the T1 birds than those fed T2, T3 and T4. It was concluded that, COLM enhanced adequate haematocrit and immune status, hypoglycaemic ability, suppresses the growth of gut pathogenic microorganisms and enhanced the growth of beneficial microorganism in broilers.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Adedapo AA Ogunmiluyi IO Adeoye AT Ofuegbe SO Emikpe BO. Evaluation of the medicinal potential of the methanol leaf extract of Chromolaena odorata in some laboratory animals. J. of Med. Plants Stud. 2016;4:29-37.

  • 2. Akinmoladun AC Ibukun EO Dan-Ologe IA. Phytochemical constituents and antioxidant properties of extracts from the leaves of Chromolaena odorata. Sci. Res. Essay 2007;2:191-194.

  • 3. Akinmutumi AH Akufo A. The effect of graded levels of dietary inclusion of Siam weed Chlomolaena odorata leaf meal in grower rabbits diet in a Tropical environment. J. of Anim. Vet. Advan. 2006;5:700-711.

  • 4. Anon. Guide to the care and use of experimental animals. Vol.1. Canadian Council on Animal Care. Ottawa Ontario Canada 1980;85-90.

  • 5. AOAC. Association of Official Analytical Chemists: Official Methods of Analysis. 6th Edition. Washington DC USA. 2000.

  • 6. Apori SO Long RJ Castro FB Orskov ER. Chemical composition and nutritive value of leaves and stems of tropical weed Chromolaena odorata. Grass Forage Sci. 2000;55:77-81.

  • 7. Bonsu FRK Kagya-Agyemang JK Kwenin WKJ Hope KN Sekyere FO. Growth Performance haematological indices and carcass characteristics of broilers fed diet containing different levels of Chromolaena odorata leaf meal. Egerton J. Sci. Technol. 2013;13:115-125.

  • 8. Bryant MP Burkey LA. Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. of Dairy Sci. 1953;36:205-217.

  • 9. Ekenyem BU Obih TKO Ohanaka AO Nwajiuba CU. Effect of partial replacement of Chromolaena odorata for soybean on haematological and serum chemistry of laying birds. Int. J. of Tropical Agric. Food Syst. 2009;33:228-232.

  • 10. Fasuyi AO Fajemilehin KSO Aro SO. Nutritional potentials of Siam weed Chromolaena odorata) leaf meal SWLM) on laying hens: biochemical and haematological implications. Pakist. J. Nutrit. 2005;45:336-341.

  • 11. Igboh MN Ikewuchi CJ Ikewuchi CC. Chemical Profile of Chromolaena odorata L. King and Robinson) Leaves. Pakist. J. Nutrit. 2009;8:521-524.

  • 12. Ikewuchi JC Ikewuchi CC. Anti-cholesterolemic Effect of Aqueous Extract of the Leaves of Chromolaena odorata L) King and Robinson Asteraceae. Potential for the Reduction of Cardiovascular Risk. The Pacific J. Sci. Technol. 2011;122:385-391.

  • 13. Jensen BB Hojberg O Mikkelsen LL Hedermann MS Cannibe N. Enhancing intestinal function to treat and prevent intestinal disease. Proceedings of 9th International Symposium Dig. Physiol. In Pigs Banff AB. Canada 20031:103-119.

  • 14. Jin LZ Ho YW Abdulla N Jalaludin S. Growth performance intestinal microbial populations and serum cholesterol of broilers fed diets containing Lactobacillus culture. Poultry Sci. 1998;77:1259-1265.

  • 15. Jiwuba PC Dauda E Ezenwaka LC Eluagu CJ. Replacement Value of Maize with Sweet Potato (Ipomoea batata) Root Meal on Growth Performance and Haematogical Characteristics of Broiler Starter Birds. Arch. Curr. Res. Int. 2016a;53:1-7.

  • 16. Jiwuba PC Ikwunze K Ume SI Nsidinanya NO. Performance apparent nutrient digestibility and cost benefit of West African dwarf goats fed dietary levels of Moringa oleifera leaf meal. J. Advan. Biol. Biotech. 2016b;8:1-9.

  • 17. Jiwuba PC Ikwunze K Dauda E Ugwu DO. Haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. Brit. Biotech. J. 2016c;15:1-7.

  • 18. Jiwuba PC Kuka TT Kadurumba OE Nwachukwu CO. Evaluation of haematological and serum biochemical characteristics of Annona muricata (Sour sop) leaf extract on broiler birds. International J. Res. Stud. Microb. and Biotech. 2017;3:6-11.

  • 19. Moses SO Akintayo O Kamil OY Labunmi L Heather EV Jessika AT William N. Chemical composition and bioactivity of the essential oil of Chromolaena odorata from Nigeria. Records Nat. Prod. 2010;41:134-137.

  • 20. NRC. Nutrient requirements for poultry. 9th Rev. Edn. National Academy Press Washington DC 1994.

  • 21. Nwokolo E. Leaf meals of cassava Manihot esculenta) and Siam weed Eupatorium odoratum) as nutrient sources in poultry diets. Nutri. Report Int. 1987;36:819-826.

  • 22. Ragab HI Abdel Ati KA Kijora C Ibrahim S. Effect of Different Levels of the Processed Lablab purpureus Seeds on Laying Performance Egg Quality and Serum Parameters. Int. J. of Poult. Sci. 2012;11:131-137.

  • 23. Sajise JH Madrid JA Kohler DD. Biology of Chromolaena odorata. Philippine J. Weed Sci. 1974;1:83-87.

  • 24. Simaraks S Chinrasri O Aengwanich W. Hematological electrolyte and serum biochemical values of the Thai indigenous chickens (Gallus domesticus) in northeastern Thailand. Songklanakarin J. Sci. Technol. 2004;26:426-430.

  • 25. Uhegbu FO Chibuzo CI Onwuegbuchulam H. Lipid lowering hypoglycemic and antioxidant activities of Chromolaena odorata L) and Ageratum conyzoides L) ethanolic leaf extracts in albino rats. J. Med. Plants Stud. 2016;42:155-159.

  • 26. Vital PG Windell LR. Antimicrobial activity and cytotoxicity of Chromolaena odorata L. f.) King and Robinson and Uncaria perrottetii A. Rich) Merr. Extracts. J. Med. Plants Res. 2009;37:511-518.

Journal information
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 283 166 11
PDF Downloads 200 115 13