Identification of hypothetical duplicate accessions of plums (Prunus domestica L.) within the slovene plant gene bank collection using molecular markers

Open access

Abstract

The main goal of the Slovene Plant Gene Bank is preservation, maintenance and evaluation of traditional cultivars and other useful genotypes. The Faculty of Agriculture and Life Sciences houses among other plant materials also numerous accessions of plums (Prunus domestica L.). Duplicates among 15 accessions were studied using six microsatellite primer pairs. These microsatellite markers revealed an average of 7.67 alleles per locus, and a range of 4 to 10 different alleles per locus. The genetic distances between studied accessions were calculated using the Dice coefficient to form a dendrogram. The six SSRs were found to be adequate for differentiating among genotypes within the collection. Among the analysed accessions no duplicates were found.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Cervera MT Cabezas JA Sancha JC Martinez de Toda F Martinez-Zapater JM. Application of AFLPs to the characterisation of grapevine Vitis vinifera L. genetic resources. A case study with accessions from Rioja (Spain). Theor. Appl. Genet. 1998; 97:51-59.

  • 2. Clarke JB Sargent DJ Bošković RI Belaj A Tobutt KR. A cherry map from the interspecific cross Prunus avium “Napoleon” × P. nipponica based on microsatellite genespecific and isoenzyme markers. Tree Genet. Genomes. 2009;5:41-51.

  • 3. Clarke JB Tobutt KR. Development and characterisation of polymorphic microsatellites from Prunus avium “Napoleon” Mol. Ecol. Notes. 2003;578-580.

  • 4. Decroocq V Hagen LS Fave MG Eyquard JP Pierronnet A. Microsatellite markers in the hexaploid Prunus domestica species and parentage lineage of three European plum cultivars using nuclear and chloroplast simple-sequence repeats. Mol. Breeding. 2004;13:135-142.

  • 5. Dice LR. Measures of the amount of ecologic association between species. Ecology. 1945;297-302.

  • 6. Donoso JM Aros D Meneses C Narvaez C Infante R. Genetic relationships in apricot (Prunus armeniaca L.) using SSR markers and their implications for breeding. J. Food Agric. Environ. 2008;6(3&4):378-382.

  • 7. Doyle JJ Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987;19(1):11-15.

  • 8. Gross BL Volk GM Richards CM Forsline PL Fazio G Chao CT. Identification of “duplicate” accessions within the USDA-ARS National Plant Germplasm System Malus collection. J. Amer. Soc. Hort. Sci. 2012;137(5):333-342.

  • 9. Guarino C Santoro S Simone LD Cipriani G. Prunus avium: nuclear DNA study in wild population and sweet cherry cultivars. Genome. 2009;52:320-337.

  • 10. Koehmstedt A Aradhya M Soleri D Smith J Polito V. Molecular characterization of genetic diversity structure and differentiation in the olive (Olea europea L.) germplasm collection of the United States Department of Agriculture. Genet. Resources Crop Evol. 2011;58:519-531.

  • 11. Laucou V Lacombe T Dechesne F Siret R Bruno JP Dessup M Dessup T Ortigosa P Parra P Roux C Santoni S Vares D Peros JP Boursiquot JM This P. High throughput analysis of grape genetic diversity as a tool for germplasm collection management. Theor. Appl. Genet. 2011;122:1233-1245.

  • 12. Lund B. Ortiz R. Skovgaard IM Waugh R. Andersen SB. Analysis of potential duplicates in barley gene bank collections using re-sampling of microsatellite data. Theor. Appl. Genet. 2003;106:1129-1138.

  • 13. Meglič V Šuštar Vozlič J Luthar Z Ferant N Čerenak A Šiško M Cvelbar J. Slovene plant gene bank and genetic resources programme. In: Meglič V. (ed.). Seednet the way ahead: book of abstracts. Ljubljana Kmetijski inštitut Slovenije p. 14 2014.

  • 14. Perrier X Jacquemoud-Collet JP. DARwin-5.0. Dissimilarity analysis and representation for Windows. CIRAD Montpellier 2005.

  • 15. Saitou N Nei M. The Neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol.Biol. Evol. 1989; 4:406-425.

  • 16. Struss S Ahmad R Southwick SM Boritzki M. Analysis of sweet cherry (Prunus avium L.) cultivars using SSR and AFLP markers. J. Amer. Soc. Hort. Sci. 2003;128(6)904-909.

  • 17. Tessier C David J This P Boursiquot JM Charrier A. Optimisation of the choice of molecular markers for varietal identification in Vitis vinifera L. Theor. Appl. Genet. 1999;98:171-177.

  • 18. Wagner HW Sefc KM. IDENTITY 1.0 Centre for Applied Genetics University of Agricultural Sciences Vienna 1999.

  • 19. Zulini L Russo M Peterlunger E. Genotyping wine and table grape cultivars from Apulia (Southern Italy) using microsatellite markers. Vitis. 2002;41:183-187.

Search
Journal information
Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 365 206 8
PDF Downloads 130 91 8