Secondary Metabolites of Hypericum L. Species as Xanthine Oxidase Inhibitors

Open access


Nine Hypericum species (H. barbatum, H. hirsutum, H. linarioides, H. olympicum, H. perforatum, H. rochelii, H. rumeliacum, H. tetrapterum and H. umbellatum) collected in Serbia were assayed for inhibitory potential against xanthine oxidase in vitro, on the commercial enzyme, and compared with allopurinol. Seven studied Hypericum species (H. barbatum, H. rochelii, H. rumeliacum, H. umbellatum, H. perforatum, H. tetrapterum and H. olympicum) inhibit commercial xanthine oxidase with an IC50 below 100 μg/mL. H. barbatum exerted the most potent inhibitory effect (IC50 = 31.84 ± 6.64 μg/mL), followed closely by H. perforatum (IC50 = 37.12 ± 4.06 μg/mL).

1. Lü JM, Yao Q, Chen C. 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB) is a potent inhibitor of xanthine oxidase: a potential therapeutic agent for treatment of hyperuricemia and gout. Biochem Pharmacol 2013; 86(9): 1328-37.

2. Radi R, Rubbo H, Bush K, Freeman B. Xanthine oxidase binding to glycosaminoglycans: kinetics and superoxide dismutase interactions of immobilized xanthine oxidase-heparin complexes. Arch Biochem Biophys 1997; 339: 125-35.

3. Hufsky F, Scheubert K, Böcker S. New kids on the block: novel informatics methods for natural product discovery. Nat Prod Rep 2014; 31: 807-17.

4. Yazaki K, Okuda T. Medicinal and aromatic plants VI. In: Bajaj, Y.P.S. (ed), Biotechnology in agriculture and forestry. Springer-Verlag, Berlin, 1994: 167-78.

5. Stojanović G, Đorđević A, Šmelcerović A. Do other Hypericum species have medical potential as St. John's Wort (Hypericum perforatum)? Curr Med Chem 2013; 20: 2273-95.

6. Smelcerovic A, Spiteller M. Phytochemical analysis of nine Hypericum L. species from Serbia and the F.Y.R. Macedonia. Pharmazie 2006; 61: 251-2.

7. Smelcerovic A, Verma V, Spiteller M, et al. Phytochemical analysis and genetic characterisation of six Hypericum species from Serbia. Phytochemistry 2006; 67: 171-7.

8. Smelcerovic A, Spiteller M, Ligon AP, et al. Essential oil composition of Hypericum L. species from Southeastern Serbia and their chemotaxonomy. Biochem Syst Ecol 2007; 35: 99-113.

9. Verma V, Smelcerovic A, Zuehlke S, et al. Phenolic constituents and genetic profile of Hypericum perforatum L. from India. Biochem Syst Ecol 2008; 36: 201-6.

10. Smelcerovic A, Zuehlke S, Spiteller M, et al. Phenolic constituents of 17 Hypericum species from Turkey. Biochem Syst Ecol 2008; 36: 316-9.

11. Đorđević A, Lazarević J, Šmelcerović A, Stojanović G. The case of Hypericum rochelii Griseb. & Schenk and Hypericum umbellatum A. Kern. essential oils: Chemical composition and antimicrobial activity. J Pharm Biomed Anal 2013; 77: 145-8.

12. Radulović N, Stankov-Jovanović V, Stojanović G, et al. Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from the Balkans. Food Chem 2007; 103: 15-21.

13. Bonkanka CX, Smelcerovic A, Zuehlke S, et al. HPLC-MS analysis and anti-oedematogenic activity of Hypericum grandifolium Choisy (Hypericaceae). Planta Med 2008; 74: 719-25.

14. Spiteller M, Özen T, Smelcerovic A, et al. Phenolic constituents and the in vitro antioxidant activity of the flowers of Hypericum venustum. Fitoterapia 2008; 79: 191-3.

15. Robson NKB. Studies in the genus Hypericum L. (Guttiferae) I. Infrageneric classification. Bull Br Mus Nat Hist (Botany) 1977; 5: 293-355.

16. Benedí J, Arroyo R, Romero C, et al. Antioxidant properties and protective effects of a standardized extract of Hypericum perforatum on hydrogen peroxide-induced oxidative damage in PC12 cells. Life Sci 2004; 75: 1263-76.

17. Havlik J, Gonzalez de la Huebra R, Hejtmankova K, et al. Xantine oxidase inhibitory properties of Czech medical plants. J Ethnopharmacol 2010; 132: 461-5.

18. Kitanov GM Hypericin and pseudohypericin in some Hypericum species. Biochem Syst Ecol 2001; 29: 171-8.

Acta Facultatis Medicae Naissensis

The Journal of Faculty of Medicine in Nis

Journal Information

Cite Score 2017: 0.27

SCImago Journal Rank (SJR) 2017: 0.140
Source Normalized Impact per Paper (SNIP) 2017: 0.240


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 78 78 24
PDF Downloads 24 24 7