Bagged Aronia Melanocarpa Tea: Phenolic Profileand Antioxidant Activity / Aronia Melanocarpa Filter Čaj: Fenolni Profil I Antioksidativna Aktivnost

Open access

Summary

While there is a large number of scientific papers reporting chemical composition and biological activities of Aronia melanocarpa, there is a lack information regarding the commercially available bagged tea. In order to supply new information on the antioxidant activity of the Aronia melanocarpa tea infusions, the aim of this study was to evaluate individual phenolic compounds which could be responsible for antioxidant activities of these beverages.

Selected anthocyanins (cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin- 3-O-arabinoside, and cyanidin-3-O-xyloside), gallic acid, caffeic acid, rutin, morin, and protocatechuic acid were simultaneously detected from commercially available tea infusions using a High Performance Liquid Chromatographic (HPLC) method. The antioxidant activity was measured using five in vitro spectrophotometric methods: 1,1-diphenyl- 2-picrylhydrazyl radical scavenging activity (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid) radical cation scavenging activity (ABTS), ferric reducing-antioxidant power (FRAP) and reduction power (RP)Fe(III) to Fe(II).

Obtained results showed that anthocyanins, predominantly of cyanidin-3-O-galactoside, are the major class of polyphenolic compounds in tea infusions. Among phenolic acids the most abundant is caffeic acid. A significant correlation between DPPH and ABTS and FRAP and RP suggested that antioxidant components in these beverages were capable scavenging free radicals and reducing oxidants.

Generally, these beverages had relatively high antioxidant capacities and could be important dietary sources of antioxidant phenolics for the prevention of diseases caused by oxidative stress.

1. Jeppsson N. The effectsof fertilizer rate on vegetative growth, yield and fruit quality, with special respect to pigments, in black chokeberry (Aronia melanocarpa) cv. “Viking”. SciHort Amsterdam 2000; 83: 127-37. http://dx.doi.org/10.1016/S0304-4238(99)00070-9

2. Oszmiański J, Sapis JC. Anthocyanins in fruits of Aronia melanocarpa (chokeberry). J Food Sci 1988; 53: 1241 -2. http://dx.doi.org/10.1111/j.1365-2621.1988.tb13577.x

3. Slimestad R, Torskangerpoll K, Nateland HS, Johannessen T, Giske NH. Flavonoids from black chokeberries, Aronia melanocarpa. J Food Compost Anal 2005; 18:61-8. http://dx.doi.org/10.1016/j.jfca.2003.12.003

4. Oszmianski J, Wojdylo A. Aronia Melanocarpaphenolics and their antioxidant activity. Eur Food Res Technol 2005; 221: 809-13. http://dx.doi.org/10.1007/s00217-005-0002-5

5. Szajdek A, Borowska EJ. Bioactive Compounds and Health-Promoting Properties of Berry Fruits: A Review. Plant Foods Hum Nutr 2008; 63:147-56. http://dx.doi.org/10.1007/s11130-008-0097-5

6. Kokotkiewicz A, Jaremicz Z, Luczkiewicz M. Aronia plants: A Review of traditional use, biological activities and perspectives for modern medicine. J Med Food 2010; 13: 255-69. http://dx.doi.org/10.1089/jmf.2009.0062

7. Ara V. The black chokeberry: a healthy fruit that will soonbe “on all tongues”? Fruit Process 2002; 12: 500-6.

8. Šavikin K, Zdunić G, Janković T, Gođevac D, Stanojković T, Pljevljakušić D. Berry fruit tea: Phenolic composition and cytotoxic activity. Food Res Int 2014; 62: 677-83. http://dx.doi.org/10.1016/j.foodres.2014.04.017

9. Shahidi F. Antioxidants in food and food antioxidants. Nahrung. 2000; 44: 158-63. http://dx.doi.org/10.1002/1521-3803(20000501) 44:3<158:: AID-FOOD158>3.0.CO;2-L

10. SRPS EN 1131: 2005, Sokovi od voća i povrća, Određivanje relativne gustine

11. AOAC method 930.15, Loss on drying (moisture) for feeds (at 135ºC for 2 hours) dry metter on oven drying for feeds (at 135ºC for 2 hours) In: Official Methods of Analysis, Association of Official Analytical Chemists, Washington, D.C., 1990: 69-70.

12. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWTFood SciTechnol 1995; 28: 25-30. http://dx.doi.org/10.1016/S0023-6438(95)80008-5

13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolonization assay. Free Radical BioMed1999; 26: 1231-7. http://dx.doi.org/10.1016/S0891-5849(98)00315-3

14. Arts MJTJ, Haenen GRMM, VossHP, Bast A. Antioxidant capacity of reaction products limit the applicability of the trolox equivalent antioxidant capacity (TEAC) assay. FoodChem Toxicol2004; 42: 45-9. http://dx.doi.org/10.1016/j.fct.2003.08.004

15. Benzie IFF, Strain JJ. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999; 299: 15-27. http://dx.doi.org/10.1016/S0076-6879(99)99005-5

16. Oyaizu M. Studies on products of browning reaction: antioxidative activity of products of browning reaction prepared from glucosamine. Jpn JNutr 1986; 44: 307-15. http://dx.doi.org/10.5264/eiyogakuzashi.44.307

17. Naczk M, Shahidi F. Extraction and analysis of phenolics in food. JChromatogr A,2004; 1054: 95-111. http://dx.doi.org/10.1016/j.chroma.2004.08.059

18. Miller JN, Miller JC. Statistics and Chemometrics for Analytical Chemistry. Pearson Education, London, 2005: 288.

19. Pravilnik o kvalitetu čaja, biljnog čaja i njihovih proizvoda, Službeni glasnik Republike Srbije br. 4/2012.

20. Apak R, Gorinstein S, Bohm V, Schaich KM, Ozyurek M, Guclu K. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure ApplChem 2013; 85: 957-98. http://dx.doi.org/10.1351/PAC-REP-12-07-15

21. Hengst C, Werner S, Muller L, Frohlich K, Bohm V. Determination of the antioxidant capacity: influence of the sample concentration on the measured values. Eur Food Res Technol 2009; 230: 249-54. http://dx.doi.org/10.1007/s00217-009-1166-1

22. Campos AM, Lissi EA. Kinetics of the reaction between 2,2-azinobis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) derived radical cation and phenols. Int J Chem Kinet 1996; 29: 219-24. http://dx.doi.org/10.1002/(SICI)1097-4601(1997)29:3<219:: AID-KIN9>3.0.CO;2-X

23. Piljac-Žegarac J, Belščak A, Piljac A. (2009). Antioxidant capacity and polyphenolic content of blueberry (Vaccinium corymbosum L.) leafinfusions. J Med Food 2009; 12:608-14. http://dx.doi.org/10.1089/jmf.2008.0081

24. Veljković JN, Pavlović AN, Mitić SS, Tošić SB, Stojanović GS, Kaličanin BM, Stanković DM, Stojković MB, Mitić MN, Brcanović JM. Evaluation of individual phenolic compounds and antioxidant properties of black, green, herbal and fruit tea infusions consumed in Serbia: spectrophotometrical and electrochemical approaches. J Food Nutr Res 2013; 52: 12-24.

25. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plantderived polyphenolic flavonoids. Free Radical Res 1995; 22: 375-83. http://dx.doi.org/10.3109/10715769509145649

26. Zheng W, Wang SY. Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries and lingonberries. J Agric Food Chem 2003; 51: 502-9. http://dx.doi.org/10.1021/jf020728u

27. Maatta-Riihinen KR, Kamal-Eldin A, Mattila PH, Gonzalez- Paramas AM, Torronen AR. Distribution and content of phenolic compounds in eighteen Scandinavian berry species. J Agric Food Chem 2004; 52: 4477-86. http://dx.doi.org/10.1021/jf049595y

28. Wu X, Gu L, Prior RL, McKay S. Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia and Sambucus and their antioxidant capacity. J Agric Food Chem 2004; 52: 7846-56. http://dx.doi.org/10.1021/jf0486850

29. Slimestad R, Torskangeroll K, Nateland HS, Johannessen T, Giske NH. Flavonoids from black chokeberries, Aronia melanocarpa. J Food Comp Anal 2005; 18: 61-8. http://dx.doi.org/10.1016/j.jfca.2003.12.003

30. Kulling SE, Rawel HM. Chokeberry (Aronia melanocarpa) - A review on the characteristic components and potential health effects. Planta Med 2008; 74: 1625-34. http://dx.doi.org/10.1055/s-0028-1088306

31. Jakobek L, Šeruga M, Krivak P. The influence of interaction among phenolic compounds on the antiradical activity of chokeberries (Aronia melanocarpa). 2011; 62: 345-52.

Acta Facultatis Medicae Naissensis

The Journal of Faculty of Medicine in Nis

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