An assessment of adaptive and antagonistic properties of Trichoderma sp. strains in vegetable waste composts

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Abstract

The experiment consisted in monitoring the count of moulds and three selected Trichoderma sp. isolates (T1 - Trichoderma atroviride, T2 - Trichoderma harzianum, T3 - Trichoderma harzianum) in vegetable (onion and tomato) waste composted with additives (straw, pig manure). Additionally, the aim of the study was to determine the type of interaction occurring between autochthonous fungi isolated from composts after the end of the thermophilic phase and Trichoderma sp. strains applied in the experiment. Number of microorganisms was determined by the plate method, next the identification was confirmed. The rating scale developed by Mańka was used to determine the type of interactions occurring between microorganisms. The greatest count of moulds in onion waste composts was noted in the object which had simultaneously been inoculated with two strains T1 - T. atroviride and T3 - T. harzianum. The greatest count of moulds was noted in the tomato waste composts inoculated with T2 - T. harzianum strain. Microscope identification revealed that Penicillum sp., Rhizopus sp., Alternaria sp. and Mucor sp. strains were predominant in onion waste composts. In tomato waste composts Penicillium was the predominant genus, followed by Rhizopus. The test of antagonism revealed the inhibitory effect of Trichoderma isolates on most autochthonous strains of moulds. Tomato waste composts proved to be better substrates for the growth and development of Trichoderma sp. isolates. The results of the study show that vegetable waste can be used in agriculture as carriers of antagonistic microorganisms.

Amann, R.I., Krumholz, L. & Stahl, D.A. (1990). Fluorescent- -oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology, Journal of Bacteriology, 172, 2, pp. 762-770.

Bal, U. & Altintas, S. (2006). A positive side effect from Trichoderma harzianum, the biological control agent: Increased yield in vegetable crops, Journal of Environmental Protection and Ecology, 7, 2, pp. 383-387.

Busko, M., Chełkowski, J., Popiel, D. & Perkowski, J. (2008). Solid substrate bioassay to evaluate impact of Trichoderma on trichothecene mycotoxin production by Fusarium species, Journal of the Science of Food and Agriculture, 88, 3, pp. 536-541.

Cavalcante, R.S., Lima, H.L.S., Pinto, G.A.S., Gava, C.A.T. & Rodrigues, S. (2008). Effect of moisture on Trichoderma conidia production on corn and wheat bran by solid state fermentation, Food and Bioprocess Technology, 1, 1, pp. 100-104. Crop Protection Products Act of 8 March 2013, Offi cial Journal 2013 Pos. 455, pp. 1-53. (in Polish)

Dłużniewska, J. (2004). The infl uence of incubation temperature on the development and biotic relations between Trichoderma spp. and pathogenic fungi, Acta Scientiarum Polonorum, Agricultura, 3, 2, pp. 257-262. (in Polish)

Domsch, K.H., Gams, W. & Anderson, T.H. (1993). Compendium of soil fungi, ed., Academic Press, San Francisco 1993.

EU Council Directive 1999/31/CE of 26 April 1999 on Waste Storage, EU Offi cial Journal 1999, L182/1, pp. 228-246.

FAO Statistics,(2008). Productions, crops. (http://faostat.fao.org/site/567/default.aspx#ancor (15.02.2008)).

Harman, G.E., Howell, C.R., Viterbo, A., Chet, I. & Lorito, M. (2004). Trichoderma species-opportunistic, avirulent plant symbionts, Nature Reviews Microbiology, 2, 1, pp. 43-56.

Howell, C.R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts, Plant Disease, 87, 1 pp. 4-10.

Malińska, K., Zabochnicka-Świątek, M. & Dach, J. (2014). Effects of biochar amendment on ammonia emission during composting of sewage sludge, Ecological Engineering, 7, pp. 474-478.

Mańka, K. (1974). Fungal communities as criterion for estimating the effect of the environment of plant disease in Poland, Zeszyty Problemowe Postępów Nauk Rolniczych, 160, pp. 9-23. (in Polish)

Martin, J.P. (1950). Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi, Soil Science, 69, pp. 215-232.

Perek, A., Krzymińska, J. & Świerczyńska, I. (2013). Comparison of the antagonistic effect of Trichoderma spp. and yeasts on pathogenic Fusarium spp. in in vitro conditions, Journal of Research and Applications in Agricultural Engineering, 58, 4, pp. 99-103. (in Polish)

Phay, N., Higashiyama, T., Tsuji, M., Matsuura, H., Fukushi, Y., Yokota, A. & Tomita, F. (1999). An antifungal compound from roots of Welsh onion, Phytochemistry, 52, pp. 271-274.

Piegza, M., Stolaś, J., Kancelista, A. & Witkowska, D. (2009). The infl uence of fungi of the Trichoderma genus on the growth of pathogenic fi lamentous fungi in biotic tests on atypical sources of carbon, Acta Scientiarum Polonorum, Biotechnologia, 8, 1, pp. 3-14. (in Polish)

Regulation of the Minister of the Environment on R10 recovery, issued on 20 January 2015, Pos. 132. (in Polish)

Rosalak, M. & Gworek, B. (2006). The state and assessment of waste handling in Poland, Ochrona Środowiska i Zasobów Naturalnych, 29, pp. 71-84. (in Polish)

Siddiquee, S., Yusof, N.A., Salleh, A.B., Tan, S.G., Bakar, F.A. & Heng, L.Y. (2010). DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modifi ed gold electrode, Sensors and Actuators B: Chemical, 147, pp. 198-205.

Skowron, K., Olszewska, H. & Pawłowska, A. (2011). The effect of composting process on changes in quantity of yeast and mould fungi population, Rocznik Ochrona Środowiska, 3, 1, pp. 1485-1506. (in Polish)

Smolińska, U., Gołębiewska, E., Kowalska, B., Kowalczyk, W. & Szczech, M. (2014). Waste materials as carriers of antagonistic Trichoderma fungi, Inżynieria i Ochrona Środowiska, 17, 1, pp. 5-20. (in Polish)

Smolińska, U., Kowalska, B., Kowalczyk, W. & Szczech, M. (2014a). The use of agro-industrial wastes as carriers of Trichoderma fungi in the parsley cultivation, Original Scientia Horticulturae, 179, 24, pp.1-8.

Sołowiej, P. (2007) An example of using a compost prism as a low-temperature source of heat. Department of electrical and power engineering, University of Warmia and Mazury in Olsztyn, Inżynieria Rolnicza, 8, 96, pp. 247-249. (in Polish)

Szczech, M., Staniszek, M., Habdas, H., Uliński, Z. & Szymański, J. (2008). Trichoderma spp. The cause of green mold on polish mushroom farms, Vegetable Crops Research Bulletin, 69, pp. 105-114.

Wang, H.X. & Ng, T.B. (2004). Isolation of allicepin, a novel antifungal peptide from onion (Allium cepa) bulbs, Journal of Peptide Science, 10, 3, pp. 173-177.

Wawrzyniak, J. & Waśkiewicz, A. (2014). Ochratoxin A and citrinin production by Penicillium verrucosum on cereal solid substrates, Food Additives and Contaminants: Part A, 31, 1, pp. 139-148.

Witkowska, D. & Maj, A. (2002). Production of lytic enzymes by Trichoderma spp. and their effect on the growth of phytopathogenic fungi, Folia Microbiologica, 47, 3, pp. 279-282.

Wojtkowiak-Gębarowska, E. (2006). Mechanisms of controlling soil phytopathogens with fungi of the Trichoderma genus, Postępy Mikrobiologii, 45, 4, pp. 261-273. (in Polish)

Wolna-Maruwka, A., Mocek-Płóciniak, A., Głuchowska, K., Schroeter-Zakrzewska, A., Borowiak, K., Niewiadomska, A., Starzyk, J. & Dach, J. (2015). Assessment of the infl uence of composts on microbiological and biochemical parameters of substrates and the morphological traits of scarlet sage, Archives of Environmental Protection, 41(3), pp. 28-38.

Yedidia, I., Srivastava, A.K., Kupalnik, Y. & Chet, I. (2001). Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants, Plant and Soil, 235, pp. 235-242.

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The Journal of Institute of Environmental Engineering and Committee of Environmental Engineering of Polish Academy of Sciences

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