Removal of copper from copper-contaminated river water and aqueous solutions using Methylobacterium extorquens modified Erzurum clayey soil

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The objective of this study was to investigate the possibility of using natural and bacteria-modified Erzurum clayey soil with Methylobacterium extorquens as an alternative to high cost commercial adsorbent materials for the removal of copper from aqueous solution. The copper concentrations in the samples of the polluted river water and CuCl2 solutions treated by the natural and bacteria-modified Erzurum clayey soil (ECS) have been determined by spectrophotometric method. Firstly, the surface of ECS was modified with M. extorquens and surface functionality was increased. Then, the adsorption of Cu (II) from solution phases was studied with respect to varying metal concentration, pH, and temperature and agitation time. The maximum adsorption of Cu (II) for natural and bacteria-modified Erzurum clayey soil was observed at pH: 5.0. At different copper concentrations, copper adsorption analysis was performed on 1 g using clay soil or modified clay soil. Maximum adsorption of Cu (II) was obtained as 45.7 and 48.1 mg g-1 at initial concentration (50 mg/50 mL) and optimal conditions by natural and bacteria-modified clay soil, respectively. The copper concentration was decreased in the substantial amount of the leachates solutions of natural and bacteria-modified clay soil. Langmuir and Freundlich isotherms were used to describe the adsorption behavior of Cu (II) ions. The results showed that modified clay soil had a high level of adsorption capacity for copper ion. The various thermodynamic parameters such as ΔG°, ΔH° and ΔS° were analyzed to observe the nature of adsorption. The structural properties of the natural and bacteria-modified-ECS have been characterized by SEM, FTIR and XRD techniques. Consequently, it was concluded that the bacteria-modified clay soil could be successfully used for the removal of the copper ions from the aqueous solutions.

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  • Ahmad A. Rafatullah M. Sulaiman O. Ibrahim M.H. Chii Y.Y. & Siddique B.M. (2009). Removal of Cu (II) and Pb (II) ions from aqueous solutions by adsorption on sawdust of Meranti wood Desalination 247 pp. 636-646.

  • Aksu Z. (2001). Equilibrium and kinetic modelling of cadmium (II) biosorption by C. vulgaris in a batch system: effect of temperature Separation and Purification Technology 21 pp. 285-294.

  • Aksu Z. & Isoglu I.A. (2005). Equilibrium and kinetic modelling of cadmium (II) biosorption by C. vulgaris in a batch system: effect of temperature Process Biochemistry 40 pp. 3031-3044.

  • Al-Asheh S. & Banat F. (2001). Adsorption of Zn (II) and Cu (II) ions by the solid waste of the olive oil industry Adsorption Science and Technology 19 pp. 117-129.

  • Amuda O.S. Giwa A.A. & Bello I.A. (2007). Removal of heavy metal from industrial wastewater using modified activated coconut shell carbon Biochemical Engineering Journal 36 pp. 174-181.

  • Bansal M. Singh D. Garg V.K. & Rose P. (2009). Use of agricultural waste for the removal of nickel ions from aqueous solutions: equilibrium and kinetics studies World Academy of Science Engineering and Technology 51 pp. 431-437.

  • Bansal R.C. & Goyal M. (2005). Activated carbon adsorption CRC Press Taylor & Francis Group LCC ISBN 0-8247-5344-5 Boca Raton Florida USA 2005.

  • Baraka A. Hall P.J. & Heslop M.J.(2007). Preparation and characterization of amine-formaldehyde-DTPA chelating resin and its use as an adsorbent for heavy metals removal from wastewater Reactive and Functional Polymers 67 pp. 585-600.

  • Benaissa H. & Elouchdi M.A. (2007). Removal of copper ions from aqueous solutions by dried sunflower leaves Chemical Engineering and Processing 46 pp. 614-622.

  • Bhatnagara A. Minocha A.K. & Sillanpaa M. (2010). Adsorptive removal of cobalt from aqueous solution by utilizing lemon peel as biosorbent Biochemical Engineering Journal 48 pp. 181-186.

  • Bhattacharyya K.G. & Gupta S.S. (2008). Adsorption of a few heavy metals on natural and modified kaolinite and montmorillonite: A review Advances in Colloid and Interface Science 140 pp. 114-131.

  • Chen X. Chen G. Chen L. Chen Y. Lehmann J. McBride M.B. & Hay A.G. (2011). Adsorption of copper and zinc by biochars produced from pyrolysis of hardwood and corn straw in aqueous solution Bioresource Technology 102 pp. 8877-8884.

  • Demirbas D. Karadag A. Aklan M. & Dogan M. (2008). Removal of copper ions from aqueous solutions by hazelnut shell Journal of Hazardous Materials 153 pp. 677-684.

  • Dhabab J.M. (2011). Removal of some heavy metal ions from their aqueous solutions by duckweed Journal of Toxicology and Environmental Health Sciences 3 6 pp. 164-170.

  • Dias J.M. Alvim-Ferraz M.C.M. Almeida M.F. Rivera-Utrilla J. & Sanchez-Polo M. (2007). Waste materials for activated carbon preparation and its use in aqueous-phase treatment: a review Journal of Environmental Management 85 pp. 833-846.

  • Elouear Z. Bouzid J. Boujelben N. Feki M. Jamoussi F. & Montiel A. (2008). Heavy metal removal from aqueous solutions by activated phosphate rock Journal of Hazardous Materials 156 pp. 412-420.

  • Freundlich H. & Hatfield H. (1926). Colloid and Capillary Chemistry Methuen and Co Ltd London 1926.

  • Gangoli N. Markey D.C. & Thodos G. (1975). In Proceedings of the Second National Conference on Complete Water Reuse: Water’s Interface with Energy Air and Solids Chicago IL AIChE. 3 pp. 270-275.

  • Ghazy S.E. & Ragab A.H. (2007). Removal of copper from water samples by sorption onto powdered limestone Indian Journal of Chemical Technology 14 pp. 507-514.

  • Gupta V.K. Jain C.K. Ali I. Sharma M. & Saini V.K. (2003). Removal of cadmium and nickel from wastewater using bagasse fly ash - a sugar industry waste Water Research 37 pp. 4038-4044.

  • Ibrahim M.N.M. Ngah W.S.W. Norliyana M.S. & Daud W.R.W. (2009). Copper (II) biosorption on soda lignin from oil palm empty fruit bunches (EFB) Clean - Soil Air Water 37 pp. 80-85.

  • Jaman H. Chakraborty D. & Saha P. (2009). A study of the thermodynamics and kinetics of copper adsorption using chemically modifi ed rice husk Clean - Soil Air Water 37 pp. 704-711.

  • Jan S. Roblot C. Courtois J. Courtois B. Barbotin J.N. & Seguin J.P. (1996). H-NMR spectroscopic determinatin of poly 3-hydroxybutyrate extracted from microbial biomass Enzyme and Microbial Technology 18 pp. 195-201.

  • Kakitani T. Hata T. Kajimoto T. Koyanaka H. & Imamura Y. (2009). Characteristics of a bioxalate chelating extraction process for removal of chromium copper and arsenic from treated wood Journal of Environmental Management 90 pp. 1918-1923.

  • Kalkan E. & Bayraktutan M.S. (2008). Geotechnical evaluation of Turkish clay deposits: a case study in Northern Turkey Environmental Geology 55 pp. 937-950.

  • Kalkan E. Nadaroglu H. & Demir N. (2012). Experimental study on the nickel (II) removal from aqueous solutions using silica fume with/without apocarbonic anhydrase Desalination and Water Treatment 44 pp. 180-189.

  • Kalkan E. & Yarbaşı N. (2013). Use of marble dust waste material for stabilization of compacted clayey soils Jokull Journal 63 5 pp. 322-344.

  • Kumar P.S. Ramalingam S. Sathyaselvabala V. Kirupha S.D. & Sivanesan S. (2011). Removal of copper (II) ions from aqueous solution by adsorption using cashew nut shell Desalination 266 pp. 63-71.

  • Langmuir I. (1918). The adsorption of gases on plane surfaces of glass mica and platinum Journal of the American Chemical Society 40 8 pp. 1361-1403.

  • Laraous S. Meniai A.H. & Bencheikh Lehocine M. (2005). Experimental study of the removal of copper from aqueous solutions by adsorption using sawdust Desalination 185 pp. 483-490.

  • Li X. Yanfeng Li Y. & Ye Z. (2011). Preparation of macroporous bead adsorbents based on poly(vinyl alcohol)/chitosan and their adsorption properties for heavy metals from aqueous solution Chemical Engineering Journal 178 pp. 60-68.

  • Nadaroglu H. & Kalkan E. (2014). Removal of copper from aqueous solution using silica fume with/without apocarbonic anhydrase Indian Journal of Chemical Technology 21 pp. 249-256.

  • Nadaroglu H. & Kalkan E. (2012). Alternative absorbent industrial red mud waste material for cobalt removal from aqueous solution International Journal of Physical Sciences 7 9 pp. 1386-1394.

  • Nadaroglu H. Kalkan E. & Celebi N. (2014). Removal of copper from aqueous solutions by using Berriasiyen-Aptian aged micritic limestone Carpathian Journal of Earth And Environmental Sciences 9 1 pp. 69-80.

  • Nadaroglu H. Kalkan E. & Celik H. (2015). Equilibrium studies of copper ion adsorption onto modified and powdered kernel of date (Fructus dactylus) International Journal of Environmental Science and Technology 12 pp. 2079-2090.

  • Nadaroglu H Kalkan E. & Demir N. (2010). Removal of copper from aqueous solution using red mud Desalination 153 pp. 90-95.

  • Najua D.T. Luqman C.A. Zawani Z. & Suraya A.R. (2008). Adsorption of copper from aqueous solution by Elais Guineensis kernel activated carbon Journal of Engineering Science and Technology 3 pp. 180-189.

  • Nohut S. Karabocek S. Guner S. & Gok Y. (1999). Extraction and spectrophotometric determination of copper (II) with S S′-bis(2-aminophenyl)oxalate Journal of Pharmaceutical and Biomedical Analysis 20 pp. 309-314.

  • Onundi Y.B. Mamun A.A. Al Khatib M.F. & Ahmed Y.M. (2010). Adsorption of copper nickel and lead ions from synthetic semiconductor industrial wastewater by palm shell activated carbon International Journal of Science and Technology 7 pp. 751-758.

  • Polowczyk I. Bastrzyk A. Kozlecki T. Rudnicki P. Sawinski W. & Sadowski Z. (2007). Application of fl y ash agglomerates in the sorption of arsenic Polish Journal of Chemical Technology 9 pp. 37-41.

  • Pons M.P. & Fuste C.M. (1993). Uranium uptake by immobilized cells of Pseudomonas strain EPS 5028 Applied Microbiology and Biotechnology 39 pp. 661-665.

  • Qadeer R. & Akhtar S. (2005). Kinetics study of lead ion adsorption on active carbon Turkish Journal of Chemistry 29 pp. 95-99.

  • Quan H. Bai H. Han Y. Kang Y. & Sun J. (2013). Removal of Cu(II) and Fe(III) from aqueous solutions by dead sulfate reducing bacteria Frontiers of Chemical Science and Engineering 7 2 pp. 177-184.

  • Ramesh A. Lee D.J. & Wong J.W.C. (2005). Thermodynamic parameters for adsorption equilibrium of heavy metals and dyes from wastewater with low-cost adsorbents Journal of Colloid and Interface Science 291 pp. 588-592.

  • Rashad M.M. Hessien M.M. Abdel-Aal E.A. El-Barawy K. & Singh R.K. (2011). Transformation of silica fume into chemical mechanical polishing (CMP) nano-slurries for advanced semiconductor manufacturing Powder Technology 205 pp. 149-154.

  • Sahan T. Ceylan H. Sahiner N. & Aktas N. (2010). Optimization of removal conditions of copper ions from aqueous solutions by Trametes versicolor Bioresource Technology 101 pp. 4520-4526.

  • Sarioglu M. Guler U.A. & Beyazit N. (2009). Removal of copper from aqueous solutions by phosphate rock Desalination 239 pp. 167-174.

  • Sarioglu M. May O.A. & Cebeci Y. (2005). Removal of copper from aqueous solutions by phosphate rock Desalination 181 pp. 303-311.

  • Seifullina I.I. & Skorokhod L.S. (1991). Spectrophotometric study of the reaction of copper (II) nickel (II) and cobalt (II) salts with 1-amino-2-hydroxy-4-naphthalenesulfonic acid Zhurnal Obshchei Khimii Russia Journal of General Chemistry 61 pp. 2005-2008.

  • Sekher K.C. Subramanian S. Modak J.M. & Natarajan K.A. (1998). Removal of metal ions using an industrial biomass with reference to environmental control International Journal of Mineral Processing 53 pp. 107-120.

  • Unlu N. & Ersoz M. (2007). Removal of heavy metal ions by using dithiocarbamatedsporopollenin Separation and Purification Technology 52 pp. 461-469.

  • Wang R. Men J. & Gao B. (2012). The adsorption behavior of functional particles modifi ed by polyvinylimidazole for Cu (II) ion Clean - Soil Air Water 40 pp. 278-284.

  • Wang S. Boyjoo Y. Choueib A. & Zhu Z.H. (2005). Removal of dyes from aqueous solutionusing fl y ash and red mud Water Research 39 pp. 129-138.

  • Yang Z. Zhang Z. Chai L. Wang Y. Liu Y. & Xiao R. (2016). Bioleaching remediation of heavy metal-contaminated soils using Burkholderia sp. Z-90 Journal of Hazardous Materials 301 pp. 145-152.

  • Zhu C.S. Wang L.P. & Chen W.B. (2009). Removal of Cu (II) from aqueous solution by agricultural by-product: Peanut hull Journal of Hazardous Materials 168 pp. 739-746.

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