The effect of temperature on the efficiency of industrial wastewater nitrification and its (geno)toxicity

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The paper deals with the problem of the determination of the effects of temperature on the efficiency of the nitrification process of industrial wastewater, as well as its toxicity to the test organisms. The study on nitrification efficiency was performed using wastewater from one of Polish chemical factories. The chemical factory produces nitrogen fertilizers and various chemicals. The investigated wastewater was taken from the influent to the industrial mechanical-biological wastewater treatment plant (WWTP). The WWTP guaranteed high removal efficiency of organic compounds defined as chemical oxygen demand (COD) but periodical failure of nitrification performance was noted in last years of the WWTP operation. The research aim was to establish the cause of recurring failures of nitrification process in the above mentioned WWTP. The tested wastewater was not acutely toxic to activated sludge microorganisms. However, the wastewater was genotoxic to activated sludge microorganisms and the genotoxicity was greater in winter than in spring time. Analysis of almost 3 years’ period of the WWTP operation data and laboratory batch tests showed that activated sludge from the WWTP under study is very sensitive to temperature changes and the nitrification efficiency collapses rapidly under 16°C. Additionally, it was calculated that in order to provide the stable nitrification, in winter period the sludge age (SRT) in the WWTP should be higher than 35 days.

Adamczyk, J., Hesselsoe, M., Iversen, N., Horn, M., Lehner, A., Nielsen, P.H., Schloter, M., Roslev, P. & Wagner, M. (2003). The isotope array, a new tool that employs substrate-mediated labeling of rRNA for determination of microbial community structure and function, Applied and Environmental Microbiology, 69, pp. 6875–6887.

Anneser, B. (2004). Biodiversity and ecophysiology of nitrifying bacteria in wastewater treatment plants. Diploma thesis, Technical University of Munich, Germany. (in German)

Anthonisen, A.C., Loehr, R.C., Prakasam, T.& Stinath, E.G. (1976). Inhibition of nitrification by ammonia and nitric acids, Journal of the Water Pollution Control Federation, 48, pp. 835–852.

Cotelle, S., Masfaraud, J.F. & Ferard, J.F. (1999). Assessment of the genotoxicity of contaminated soil with the Allium/Vicia micronucleus and the Tradescantia-micronucleus assays, Mutation Research, 426, pp. 167–171.

Daims, H., Stoecker, K. & Wagner, M. (2005). Fluorescence in situ hybridization for the detection of procaryotes, in: Advanced methods in molecular microbial ecology, Osborn, A.M., Smith, C.J. (Eds.), Bios-Garland, Abingdon, UK, pp. 213–239.

Daims, H., Nielsen, P.H., Nielsen, P.H., Schleifer, K.H. & Wagner, M. (2001). In situ characterization of Nitrospira-like nitrite oxidizing bacteria active in wastewater treatment plants, Applied and Environmental Microbiology, 67(11), pp. 5271–5284.

Dokianakis, S.N., Kornaros, M. & Lyberatos, G. (2006). Impact of five selected xenobiotics on isolated ammonium oxidizers and on nitrifying activated sludge, Environmental Toxicology, 21 (4), pp. 310–316.

El Hajjouji, H., Pinelli, E., Guiresse, M., Merlina G., Revel, J.-C. & Hafidi, M. (2007). Assessment of the genotoxicity of olive mill waste water (OMWW) with the Vicia faba micronucleus test, Mutation Research, 634, pp. 25–31.

Ghyoot, W. & Verstraete, W. (2000). Reduced sludge production in a two-stage membrane-assisted bioreactor, Water Research, 34(1), pp. 205–215.

Ghyoot, W. & Verstraete, W. (1999). Nitrogen removal from sludge reject water with a membrane – assisted bioreactor, Water Research, 33(1), pp. 23–32.

Grant, W. (1994). The present status of higher plant bioassay for the detection of environmental mutagens, Mutation Research, 310, pp.175–185.

Greuter, D., Loy, A., Horn, M. & Rattei, T. (2016). ProbeBase – an online resource for rRNA-targeted oligonucleotide probes and primers: new features 2016, Nucleic Acids Research 10.1093/nar/gkv1232.

Wen, J., Jia, X., Pan, L., Wang C. & Mao, G. (2005). Nitrifying treatment of wastewater from fertilizer production in a multiple airlift loop bioreactor, Biochemical Engineering Journal, 25, pp. 33–37.

Juretschko, S., Timmermann, G., Schmid, M., Schleifer, K.H., Pommerening-Roser, A. & Wagner, M. (1998). Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations, Applied and Environmental Microbiology, 64, 3042–3051.

Hagopian, D.S. & Riley, J. (1998). A closer look at the bacteriology of nitrification, Aquacultural Engineering, 18, pp. 223–244.

Henglong, X., Weibo, S. & Alan, W. (2004). An investigation on the tolerance to ammonia of the marine ciliate Euplotes vannus (Protozoa, Ciliphora), Hydrobiologia, 519(1–3), pp. 189–195.

Kishino, H., Ishida, H., Iwabu, H. & Nakano, I. (1996). Domestic wastewaters reuse using a submerged membrane bioreactor, Desalination, 106, pp. 115–119.

Mobarry, B.K., Wagner, M., Urbain, V., Rittmann, B.E. & Stahl, D.A. (1996). Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria, Applied and Environmental Microbiology, 62, pp. 2156–2162.

Nagaoka, H. (1999). Nitrogen removal by submerged membrane separation activated sludge process, Water, Science and Technology, 39(8), pp. 107–114.

OSPAR Commission, 2002: Survey on Genotoxicity Test Methods for the Evaluation of Waste Water within Whole Effluent Assessment, ISBN 1-904426-02-6.

Popel, H.J. & Fischer, A. (1998). Combined influence of temperature and process loading in the effluent concentration of biological treatment, Water Science and Technology, 38(8–9), pp. 129–136.

Popel, H.J. & Kristeller, W. (1996). Post-denitrification at the Frankfurt-Niederrad wastewater treatment plant by fluidizedbed technology, in: Advanced Wastewater Treatment – nutrient removal and anaerobic processes – IAWQ-NVA-Conference, September 23–25, Amsterdam, pp. 153–162.

Puigagut, J., Salvado H. & Garcia, J. (2005). Short-term harmful effects of ammonia nitrogen on activated sludge microfauna, Water Research, 39, pp. 4397–4404.

Ukropec, R., Kuster, B.F.M., Schouten, J.C. & van Santen, R.A. (1999). Low temperature oxidation of ammonia to nitrogen in liquid phase, Applied Catalysis B: Environmental, 23, pp. 45–57.

Wagner, M., Rath, G., Amann, R., Koops H.-P. & Schleifer, K.-H. (1995). In situ identification of ammonia-oxidizing bacteria, Systematic and Applied Microbiology, 18, pp. 251–264.

Archives of Environmental Protection

The Journal of Institute of Environmental Engineering and Committee of Environmental Engineering of Polish Academy of Sciences

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