The knee joint is not only the most important and complex structure to present during anatomy lectures, but also a significant region for radiologists and orthopedicians. The objectives of this paper are: 1) to evaluate the efficacy of plastinated specimens by measuring colorimetric differentiation and 2) to compare the anatomical structures of the images obtained from computed tomography (CT) and silicone plastination cross-sections and perform three-dimensional (3D) reconstructed models of the specimens. A total of 16 knee joints selected from the 10% formalin fixed/dissected group (n=6), non-fixed/dissected group (n=6) and non-dissected whole knee joint group (n=4) were scanned by CT and plastinated by using the S10B silicone technique. The color changes of the structures were quantitatively determined with the colorimeter device. Plastinated whole knee joints were cut transversally with a band saw and compared with CT images. Finally, 3D reconstruction of the knee joints was performed from CT images with the 3D reconstruction program. There were statistically significant differences between the fixed and non-fixed groups in terms of color changes (p<0.001). The anatomical structures were identified and matched in the cross-sections of plastinates and corresponding CT images. It was observed that plastinated samples were elastic, durable and non-hazardous specimens to use in the veterinary orthopedics and anatomy courses and lectures. It has been found that fixation was useful for the final product morphology. It is thought that the 3D images obtained from this study will support comprehension of the relationships between the bones, muscles, and ligaments.
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1. Baird DK, Hathcock JT, Rumph PF, Kincaid SA, Visco DM: Low-field magnetic resonance imaging of the canine stifle joint: normal anatomy. Vet Radiol Ultrasoun 1998, 39:87-97.
2. Soler M, Murciano J, Latorre R, Belda E, Rodríguez MJ, Agut A: Ultrasonographic, computed tomographic and magnetic resonance imaging anatomy of the normal canine stifle joint. Vet J 2007, 174:351-361.
3. Estai M, Bunt S: Best teaching practices in anatomy education: A critical review. Ann Anat 2016, 208:151-157.
4. Samii VF, Dyce J: Computed tomographic arthrography of the normal canine stifle. Vet Radiol Ultrasoun 2004, 45:402-406.
5. Latorre RM, Rodriguez LM: In search of clinical truths: equine and comparative studies of anatomy. Equine Vet J 2007. 39:263-268.
6. Neha, Lalwani S, Dhingra R: Plastinated knee specimens: a novel educational tool. J Clin Diagn Res 2013, 7:1-5.
7. Deruddere KJ, Milne ME, Wilson KM, Snelling SR: Magnetic resonance imaging, computed tomography, and gross anatomy of the canine tarsus. Vet Surg 2014, 43:912-919.
8. Ober CP, Freeman LE: Computed tomographic, magnetic resonance imaging, and cross-sectional anatomic features of the manus in cadavers of dogs without forelimb disease. Am J Vet Res 2009, 70:1450-1458.
9. Rycke LM, Gielen IM, Bree H, Simoens PJ: Computed tomography of the elbow joint in clinically normal dogs. Am J Vet Res 2002, 63:1400-1407.
10. Vekens EV, Bergman EHJ, Vanderperren K, Raes EV, Puchalski SM, Bree HJ, Saunders JH: Computed tomographic anatomy of the equine stifle joint. Am J Vet Res 2011, 72:512-521.
11. Sora MC, Genser-Strobl B, Radu J, Lozanoff S: Three-dimensional reconstruction of the ankle by means of ultrathin slice plastination. Clin Anat 2007, 20:196-200.
12. Sora MC, Matusz P: General considerations regarding the thin slice plastination technique. Clin Anat 2010, 23:734-736.
13. Oliveria AC, Balaban MO: Comparison of a colorimeter with a machine vision system in measuring color of gulf of Mexico sturgeon fillets. Appl Eng Agric 2006, 22:583-587.
14. Turan E, Gules O, Kilimci FS, Kara ME, Dilek OG, Sabanci SS, Tatar M: The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy. Ann Anat 2017, 209:11-17.
15. Shigue DA, Rahal SC, Schimming BC, Santos RR, Vulcano LC, Linardi JL, Teixeira CR: Evaluation of the marsh deer stifle joint by imaging studies and gross anatomy. Anat Histol Embryol 2015, 44:468-474.
16. Latorre RM, Garcia-Sanz MP, Moreno M, Hernández F, Gil F, López O, Ayala MD, Ramírez G, Vázquez JM, Arencibia A, Henry RW: How useful is plastination in learning anatomy. J Vet Med Educ 2007, 34:172-176.
17. Bakıcı C, Akgun RO, Ekim O, Oto Ç, Özen D, Bilsay M: Is kaiserling solution a convenient fixative for mammalian organ specimens? evaluation of morphometric, colorimetric and volumetric properties. Bulg J Vet Med 2017, 20:62-67.
18. Riederer BM: Plastination and its importance in teaching anatomy. Critical points for long-term preservation of human tissue. J Anat 2014, 224:309-315.
19. Ekim O, Hazıroğlu RM, İnsal B, Bakıcı C, Akgün RO, Tunalı S: A modified S10B silicone plastination method for preparation and preservation of scaled reptile specimens. Vet J Ankara Univ 2017, 64:155-160.